骨髓间充质干细胞改善 APP/ PS1 模型小鼠认知功能的机制.

BACKGROUND: Studies have shown that bone marrow mesenchymal stem cell therapy can effectively fill the neurons lost in Alzheimer’s disease patients, but the specific mechanism of action needs to be further clarified. OBJECTIVE: To explore the therapeutic effects of bone marrow mesenchymal stem cells in APP/PS1 double transgenic mice with Alzheimer’s disease and its mechanism. METHODS: APP/PS1 double transgenic mice were selected as the research objects and were randomly divided into model and bone marrow mesenchymal stem cell groups (n=10 per group). Ten wild-type C57BL/6 mice of the same month and the same sex were selected as the normal control group. The mice were treated with bone marrow mesenchymal stem cells (1×106 /time) or normal saline, and repeated administration once a week, for 2 months via nasal cavity. Morris water maze test was applied to examine cognitive function of mice. Western blot assay was used to detect the expression of related factors of pathological markers, inflammatory molecules and oxidative stress proteins in the brain tissues of mice. RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cells effectively improved the cognitive dysfunction of Alzheimer’s disease mice, increased the expression of APP protein and decreased expression of phosphorylated tau protein, reduced the expression of Toll like receptor 2, inducible nitric oxide synthase, nuclear transcription factor kappa B and increased the expression of nuclear factor erythroid 2-related factor 2 and heme oxygenase-1. It is concluded that bone marrow mesenchymal stem cells have a potential role in the treatment of Alzheimer’s disease; the mechanism exerts the effects of the anti-inflammation and antioxidation through nuclear factor erythroid 2-related factor 2/heme oxygenase-1 signaling pathway. [ABSTRACT FROM AUTHOR]