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BACKGROUND: There is still controversy whether human bone marrow mesenchymal stem cells can maintain their biological characteristics, energy metabolism patterns, and multidirectional differentiation potential after long-term expression in vitro. Further comprehensive and systematic research is needed. OBJECTIVE: To evaluate the effects of long-term expansion in vitro on the biological characteristics of mesenchymal stem cells. METHODS: Human bone marrow mesenchymal stem cells cultured to passage 5, 10, and 15 in vitro. MTT assay was used to detect cell proliferation ability. Flow cytometry was used to detect cell cycle. The multi-differentiation potential of mesenchymal stem cells was detected by inducing to adipogenic, osteogenic and chondrogenic differentiation. Cell migration and invasion abilities were detected by scratch test and Transwell assay. The mitochondrial oxidative phosphorylation and glycolysis function were analyzed using energy metabolism analyzer. The cell senescence was detected by senescence-associated β-galactosidase staining. The expression levels of p21, p16, and p53 proteins were detected by western blot assay. RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cells at passages 5, 10, and 15 grew adherently; the volume of passage 15 mesenchymal stem cells increased and its proliferation ability decreased; the percentage of S-phase cells decreased (P < 0.05). With the increase of culture passages, the migration and invasion abilities decreased gradually (P < 0.05). There was no significant difference in the differentiation potential, demonstrated by adipogenic, osteogenic and chondrogenesis induction. The ability of oxidative phosphorylation of mitochondria and glycolysis decreased gradually (P < 0.05). The number of senescence-associated β-galactosidase-positive cells increased with the increase of passages (P < 0.05), and the expression of senescence protein p21, p16, and p53 increased gradually (P < 0.05). The results indicated that the biological characterization of mesenchymal stem cells changed after long-term in vitro expansion. Mesenchymal stem cells cultured over 10 passages may have a reduced activity due to increasing senescence. Therefore, bone marrow mesenchymal stem cells cultured less than 10 passages are suitable for clinical research/therapy. [ABSTRACT FROM AUTHOR]

Copyright of Science & Technology of Food Industry is the property of Science & Technology of Food Industry Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Anhui Agricultural Sciences is the property of Journal of Anhui Agricultural Sciences Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Anhui Agricultural Sciences is the property of Journal of Anhui Agricultural Sciences Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Medicine & Health is the property of Journal of Modern Medicine & Health and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In January 2024, Cancer Statistics, 2024 was published in CA: A Cancer Journal for Clinicians. This report estimated cancer cases and deaths in 2024 and described the secular trends of major cancers over recent decades. We summarized the report and integrated the latest data on the cancer burden in China to compare the prevalence cancer between two countries. We also comprehensively interpreted the underlying reasons for these trends to provide insights for cancer prevention and control strategies in China. [ABSTRACT FROM AUTHOR]

Copyright of Lubrication Engineering (0254-0150) is the property of Editorial Office of LUBRICATION ENGINEERING and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Resources Development & Market is the property of Sichuan Resource Development & Market Magazine Co., Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To explore the application value of Bobath therapy combined with the suspension technique in the rehabilitation treatment of children with cerebral palsy from the aspects of skeletal muscle development, gross motor function and limb balance. Methods A total of 98 children with cerebral palsy in Zigong Maternal and Child Health Hospital were selected from March 2021 to March 2023. According to the 1∶1 control principle, they were randomly divided into a control group and a study group using a computer random number table method, with 49 cases in each group. The control group was treated with Bobath therapy, and the study group was treated with Bobath therapy combined with suspension technology for 3 months. The therapeutic effects of the two groups were compared, as well as the gross motor function measurement scale (GMFM-88), leg skeletal muscle development [thickness of the anterior tibial muscle, medial gastrocnemius muscle, and soleus muscle (MT), muscle bundle length (FL) ], Berg balance scale (BBS), cerebral artery blood flow [Mean blood flow velocity (Vm) in the anterior cerebral artery (ACA), middle cerebral artery (MCA) and posterior cerebral artery (PCA) ], Fwave amplitude and stimulation threshold, daily living ability evaluation scale for children with cerebral palsy (ADL), and modified Ashworth spasticity scale (MAS) before treatment, at 1 month and 3 months after treatment. Results The total effective rate of the study group was 89.80% higher than that of the control group 73.47% (P < 0.05). At the 1st and 3rd month of treatment, MT and FL of the anterior tibial muscle, medial gastrocnemius muscle and soleus muscle in 2 groups were higher than before treatment, and the study group was higher than the control group (P < 0.05). At the 1st and 3rd month of treatment, GMFM-88, BBS and ADL scores in 2 groups were higher than before treatment, and those in study group were higher than those in control group (P < 0.05). At the first and third month of treatment, Vm of ACA, MCA and PCA in 2 groups was higher than before treatment, and that in study group was higher than that in control group (P < 0.05). At the first and third months of treatment, the F wave amplitude of the study group was lower than that of the control group, and the F wave stimulation threshold was higher than that of the control group (P < 0.05). At the first and third month of treatment, MAS scores in the 2 groups were lower than before treatment, and those in the study group were lower than those in the control group (P < 0.05). Conclusion The Bobath therapy combined with suspension technique has a definite effect on children with cerebral palsy, which can promote the development of skeletal muscle in the lower leg, restore gross motor function, improve balance ability, and improve cerebral artery blood flow. [ABSTRACT FROM AUTHOR]

Copyright of China Brewing is the property of China Brewing Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Engineering Geology / Gongcheng Dizhi Xuebao is the property of Journal of Engineering Geology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Laboratory Animal & Comparative Medicine is the property of Laboratory Animal & Comparative Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Electric Power Engineering Technology is the property of Editorial Department of Electric Power Engineering Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Medical Equipment Journal is the property of Chinese Medical Equipment Journal Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Medicine & Health is the property of Journal of Modern Medicine & Health and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Arid Land Geography is the property of Chinese Academy of Sciences, Xinjiang Institute of Ecology & Geography and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To investigate the effect and mechanism of HBV 一 stimulated HTR8 一 S/Vneo mitochondrial antiviral signaling protein (MAVS) signaling pathway on the immune response of peripheral blood mononuclear cell (PBMC) to hepatitis B vaccine. Methods The HTR8 - S/Vneo were co 一 incubated with serum of HBV - DNA positive patients. MAVS siRNA transfection was hereby applied for silencing of MAVS and MAVS plasmid transfection was applied for overexpression of MAVS in HTR8 一 S/Vneo. We then cocultured them with human immune cells assembly PBMC and subsequently stimulated with hepatitis B vaccine. The mRNA and protein expression levels of MAVS, NF - kB, pNF - kB, IRF3 and pIRF3 in HTR8 一 S/Vneo were detected by reverse transcription 一 quantitative real 一 time PCR (RT 一 qPCR) and flow cytometry (FCM). The levels of IL - 2, IL - 6, IFN 一 p and IL 一 17 were detected by enzyme - linked immunosorbent assay (ELISA), and the proportion of CD4+ T cell were detected by flow cytometry. The data were analyzed for t test. Results HBV down 一 regulated the mRNA expression levels of MAVS, NF - kB, IRF3 (£ 二 6. 66, P 二 0. 003; £ 二 14. 18, P < 0. 001; £ 二 3. 70, P 二 0. 021) and the protein expression levels of MAVS, NF - kB, pNF - kB(£ 二 3. 42, P = 0. 042; £ 二 4. 23, P = 0. 013; £ 二 4. 86, P - 0. 008), and inhibited the production of IFN - p(£ 二 9. 83, P = 0. 010). Silencing MAVS down 一 regulated the mRNA and protein expression of MAVS(£ 二 31. 20, P < 0. 001; £ 二 6. 53, P = 0. 023), inhibited the expression of IFN - p(£ 二 5. 46, P =0. 032), and reduced the proportion of CD4+ T cells in PBMC(£ 二 9. 07, P = 0. 001). Overexpression of MAVS induced IFN 一 B production(£ 二一 34. 26, P < 0. 001; t - -5. 11, P = 0, 036), but the proportion of CD4 * T cells did not show significant effect (/二 一 0. 73, P 二 0. 506). Conclusion HBV can inhibit the MAVS signaling pathway in HTR8 一 S/Vneo. Overexpression of MAVS could promote the immune response to hepatitis B vaccine in PBMC. [ABSTRACT FROM AUTHOR]

Copyright of East China Geology / Huadong dizhi is the property of Editorial Department of East China Geology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Applied Entomology is the property of Chinese Journal of Applied Entomology, Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Data Acquisition & Processing / Shu Ju Cai Ji Yu Chu Li is the property of Editorial Department of Journal of Nanjing University of Aeronautics & Astronautics and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Water Conservancy & Electric Power Technology & Application is the property of Omniscient Pte. Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Test & Measurement Technology is the property of Publishing Center of North University of China and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To compare the short⁃term efficacy and safety of low temperature plasma radiofrequence ablation (LA) vs. high voltage long duration pulsed radiofrequency (HL⁃PRF) in the treatment of cervicogenic headache (CEH). Methods Eighty patients with cervicogenic headache were divided two groups： one treated with low temperature plasma radiofrequence ablation group as group L (n = 50) and the other treated with high voltage long duration pulsed radiofrequency group as group H (n = 30). The two groups were compared in terms of the score by the Visual Analog Scale, score by the Range of Motion Scale, postoperative pain relief rate, postoperative complication incidence, 1, 4, 12, and 24 weeks after the operation, as well as the therapeutic effectiveness and safety. Results Both groups showed significantly lower VAS and ROM scores after operation (P < 0.05). Compared with group L, group H had significantly lower VAS (all P < 0.05) but insignificantly lower ROM scores (all P > 0.05) at each time point. At T4, group H had significantly higher therapeutic effectiveness rate (P < 0.05) and higher incidence of scalp numbness at T3, as compared to group L (P < 0.05). Conclusion The low temperature plasma radiofrequency ablation and high voltage long duration pulsed radiofrequency both are effective and safe in the treatment of CEH, the latter approach can achieve better therapeutic effectiveness 24 weeks after the operation than the former. [ABSTRACT FROM AUTHOR]

Copyright of Science & Technology of Food Industry is the property of Science & Technology of Food Industry Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of South China Agricultural University is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Non⁃occlusive mesenteric ischemia (NOMI) is a series of disorders of digestive tract caused by local blood perfusion damage, which is caused by vasospasm. As an extremely dangerous disease, NOMI is also a common intestinal complication in intensive care unit, especially in patients with hemodynamic instability after cardiac surgery, and the mortality is high. Cardiac surgery aims to improve the prognosis of patients, while the complication of NOMI is easy to be ignored, which hindered the recovery of postoperative patients. Due to the rapid development and lack of specific symptoms and signs, NOMI is easily misdiagnosed as other gastrointestinal complications. Therefore, timely and effective diagnosis of NOMI is the most important factor affecting the prognosis of post cardiac surgery. This review systematically summarized the related research on NOMI after cardiac surgery, and comprehensively concluded the cause, mechanism, treatment as well as prognosis of NOMI. Then we emphasized on the latest progresses of diagnostic methods for NOMI, in order to provide directions on the accurate identification and timely intervention of NOMI in clinical work. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Nanjing Medical University: Natural Sciences is the property of Journal of Nanjing Medicial University (NMU) Medical Publications Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Mining & Metallurgy (10057854) is the property of Beijing Research Institute of Mining & Metallurgy Technology Group and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chemistry & Industry of Forest Products is the property of Chemistry & Industry of Forest Products Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Machine Tool & Hydraulics is the property of Guangzhou Mechanical Engineering Research Institute (GMERI) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To investigate the predictive value of D-dimer （D-D）, lactic acid, and soluble platelet endothelial adhesion molecule-1（ sPECAM-1） in patients with sepsis-associated disseminated intravascular coagulation （DIC）. Methods According to the prognosis of patients at 28 days, 300 patients with sepsis-related DIC in the ICU of the Affiliated Hospital of Xuzhou Medical University were gathered and split into death and survival groups. The two groups' general statistics, APACHEⅡ score, serum D-D, lactate, and sPECAM-1 levels were compared. The operating characteristic curve （ROC） was used to evaluate the predictive value of serum D-D, lactate, and sPECAM-1 on the poor prognosis of patients with sepsis-related DIC. Results The 28-day mortality rate of patients with sepsis-related DIC was 22%, and the death group had higher APACHEⅡ scores, serum D-D, lactate, and sPECAM-1 levels than the survival group （P < 0.05）. Multivariate logistic regression results showed that serum D-D, lactic acid and sPECAM-1 were risk factors for 28-day death in patients with sepsis-related DIC. The AUC of serum D-D, lactic acid, and sPECAM-1 was 0.863 （95%CI: 0.768 ~ 0.958）, 0.831 （95%CI: 0.734 ~ 0.928）, 0.774 （95%CI: 0.653 ~ 0.896）, respectively. The AUC of the combination of serum D-D, lactic acid and sPECAM-1 was 0.965（ 95%CI: 0.928 ~ 1.000）, with the sensitivity 0.864 and the specificity 0.974, each of which were greater than the individual predictions. Conclusion In patients with sepsis-related DIC, higher serum D-D, lactate, and sPECAM-1 levels were highly correlated with 28-day death. In patients with sepsis-related DIC, higher serum D-D, lactate, and sPECAM-1 levels were highly correlated with 28-day death. The combination of serum D-D, lactate, and sPECAM-1 levels provided a high predictive value for the prognosis of sepsis-related DIC and might be employed in therapeutic treatment. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of coenzyme Q10 combined with tegrel on unstable angina pectoris of coronary heart disease and its influence on coronary blood flow parameters. 128 patients with unstable angina pectoris of coronary heart disease admitted to our hospital from October 2020 to October 2022 were selected as the study subjects. All patients were divided into observation group and matched group by random number table method, with 64 patients in each group. The patients in the matched group were treated with conventional western medicine. The observation group added coenzyme Q10 combined with tegrel on the basis of the matched group to compare the clinical treatment effect of the two groups, Visual Analogue Pain Scale (VAS) was used to assess the pain of angina pectoris in the two groups, and the daily attack frequency, duration of each time, and daily amount of nitroglycerin were compared. The cardiac function indexes and coronary blood flow parameters of the two groups were measured before and after treatment. Results: The total effective rate of treatment in the observation group was higher than that in the matched group (P<0.05); The pain score, daily attack frequency, duration of each time and daily nitroglycerin dosage in the observation group were significantly lower than those in the matched group (P; There was no difference in the 6-minute walking distance, LVEF and BNP between the two groups before treatment (P>0.05). After treatment, the 6-minute walking distance and LVEF of the two groups increased, and the level of BNP in the observation group was higher than that in the matched group, while the level of BNP in the observation group was lower than that in the matched group (P<0.05); There was no difference between the two groups before treatment in terms of diastolic time velocity integral, left anterior descending systolic peak velocity, left anterior descending diastolic peak velocity, and coronary flow velocity reserve value (P>0.05). After treatment, the two groups increased in terms of diastolic time velocity integral, left anterior descending systolic peak velocity, left anterior descending diastolic peak velocity, and coronary flow velocity reserve value, and the observation group was higher than the matched group (P<0.05). Conclusion: Coenzyme Q10 combined with tegrel has a significant effect on the treatment of unstable angina pectoris of coronary heart disease. It can reduce the degree of angina pectoris, reduce the frequency, duration and nitroglycerin consumption of patients, improve the level of cardiac function, improve coronary blood flow parameters, and is worthy of clinical application and promotion. [ABSTRACT FROM AUTHOR]

AIM: To investigate the effect and possible mechanism of sakuranetin(SK)on 2,4,6-trinitroben-zenesulfonic acid (TNBS) -induced Crohn disease (CD) -like colitis in mice. METHODS: A total of 24 C57BL/6J mice were randomly divided into a control, model (TNBS), and SK (20 mg-kg'-d"1) treatment groups(TNBS+SK), with eight mice in each group. Colitis symptoms were evaluated using the disease activity index(DA1) and body weight change. The colon injury was evaluated based on colon length, histological inflammation score, and levels of intestinal mucosal inflammatory mediators(TNF-ot, IL-6, 1L-17A, and IL-1 p). The intestinal barrier function was assessed by measuring the levels of fluorescein isothiocyanate-dextran 4 kD(FD4)and intestinal fatty acid binding protein (1-FABP)in the peripheral blood, colonic transcpitliclial electric resistance (TEER), and intestinal bacterial translocation rate. (tCiic Ontology ((;O)and Kyoto Encyclopedia of Genes and Genomes(KEG(;) cnrichmciit analyses were performed to predict the potential pathways and SK mechanisms, followed by validation through animal experiments. RESULTS: The DAI score and body weight loss in the l -BS+SK group were significantly lower than those in the group hut higher than those in the control group (P<0. 05). The TMJS+SK group showed a significant reduction in colonic shortening, histological iiiflammatory scores, and levels of colonic mucosal inflammatoiy mediators compared to the TABS group, hut higher than those in the control group (P<0. 05). The levels of FD4 and 1-1ABP in the peripheral blood of the TMiS+SK group were significantly lower than those of the TJSBS group, hut higher than those of the control group(P

Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Food Science & Technology & Economy is the property of Grain Science & Technology & Economy Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)