To observe the expression levels of serum chorionic gonadotropin (β-HCG), progesterone (P) and estradiol (E2) in pregnant women with early embryo stop development, and analyze the influencing factors of early embryo stop development. From April 2020 to January 2022, 94 patients with early embryo stop development who were admitted to Changsha Fourth Hospital were selected as the study group, and 60 healthy pregnant women who came to Changsha Fourth Hospital for physical examination were randomly selected as the control group. The expression levels of serum β-HCG, P and E2 in the control group and the study group were compared. The correlation between β-HCG, E2 and P was analyzed by Pearson correlation analysis. Multivariate logistic regression were used to analyze the influencing factors of early embryo stop development. The levels of β-hCG, P and E2 in the study group were lower than those in the control group (P<0.05). Pearson correlation analysis showed that β-HCG was positively correlated with E2 and P, and E2 was positively correlated with P (P<0.05). Univariate analysis showed that age, use of emergency contraception during pregnancy, recent exposure to toxic and harmful substances, average monthly family income, previous abortion, folic acid supplementation, sleep duration <6 h/d, TORCH infection and daily exposure to ionizing radiation were associated with early embryo stop development (P<0.05). The results of multivariate regression showed that: low levels of β-HCG, P and E2, use of emergency contraception during pregnancy, older age, regular sleep duration <6 h/d, TORCH infection, recent exposure to toxic and harmful substances and daily exposure to ionizing radiation ≥6 h were risk factors for early embryo stop development. However, folic acid supplementation and high average monthly family income were the protective factors for early embryo stop development (P<0.05). The low levels of β-HCG, P and E2 in pregnant women with early embryo stop development are influenced by age, use of emergency contraception during pregnancy, average monthly family income, TORCH infection, recent exposure to toxic and harmful substances, previous history of abortion, daily exposure to ionizing radiation and sleep duration. Folic acid supplementation is a protective factor against early embryo stop development. [ABSTRACT FROM AUTHOR]