mNGS 在老年社区获得性肺炎病原学诊断的临床价值.

Objective To analyze the clinical value of metagenomic next-generation sequencing (mNGS) in the pathogen detection of elderly patients with community-acquired pneumonia (CAP). Methods A total of 112 elderly CAP patients were included in the study. The detection efficiency of pathogens using mNGS was compared with conventional microbiological tests (CMTs). Statistical analyses were conducted using Fisher′s exact test and ROC curves to evaluate the diagnostic differences between mNGS and CMTs. Results The overall diagnostic rate of mNGS was significantly higher than that of CMTs (94.6% vs. 65.2%, P＜0.001). The detection time for mNGS was significantly shorter than that for CMTs [ (26.3±5.9)h vs. (85.5±6.4)h; t=21.034, P＜0.05]. mNGS detected a higher number of bacterial, viral, fungal, and atypical pathogens compared to CMTs. The detection rate for mixed infections with mNGS was 76.4%, significantly higher than CMTs′ 60.3% (χ²=6.851, P＜0.05). Of the detected pathogens, there were 64 cases of agreement, 35 cases of partial agreement, and 13 cases of complete disagreement between mNGS and CMTs. Conclusion mNGS demonstrates superior capability in detecting a broader spectrum of pathogens in elderly patients with community-acquired pneumonia, highlighting its significant clinical value. [ABSTRACT FROM AUTHOR]