To elucidate the function and reproductive regulation mechanism of the Vitellogenin(GmVg) gene in Grapholita molesta, a significant pest of fruit trees, and to provide a theoretical basis for further research on the function of the GmVg gene, in this study, GmVg was cloned and identified and a comprehensive bioinformatics analysis was performed. The expression patterns of GmVg at various developmental stages, across sexes, and in different tissues were examined using quantitative reverse transcription PCR(qRT-PCR). The results revealed that the full-length of GmVg gene spanned 5 573 base pairs(bp), with an open reading frame(ORF) of 5 364 bp that encoded 1 787 amino acids. The signal peptide consisted of 15 amino acids, and the molecular formula was C9041H14090N2544O2743S57. The percentage of the amino acids alanine(Ala), glutamine(Gln), and serine (Ser) was large and they comprised 8.2%, 7.9%, and 7.8% of the protein, respectively. The protein contained 187 acidic residues(Asp+Glu) and 204 basic residues(Arg+Lys). Bioinformatics predictions indicated that the molecular weight of the GmVg protein was 204.1 ku, with an isoelectric point of 8.79, and it featured three characteristic conserved domains: Vitellogenin-N, DUF1943, and VWD. The expression of GmVg was markedly higher in adults compared to that in eggs, larvae, and pupae, reaching its peak 24 h post-emergence. The gene displayed sex-specific expression, with negligible levels in males. Within female adults, tissue-specific patterns were noted in head, ovary, and fat body; the expression in the fat body was substantially higher than in the head and ovary-21.49-fold and 16.4-fold greater, respectively. [ABSTRACT FROM AUTHOR]