The combined pollution of heavy metals and pesticides is ubiquitous in the environment, however,researches on their joint toxicity on organisms are still insufficient. Toxicity tests were conducted in our study to evaluate acute toxicity effects of single and mixed samples contaminated by cartap, lead(Pb)and chromium(Cr)(cartap-Pb and cartap-Cr mixed at the ratios of 1:1, 1:2 and 2:1, cartap-Pb-Cr mixed at the ratios of 1:1:1, 1:1:2, 1:2:1 and 2:1:1)on Vibrio fischeri under 15 min and 30 min, respectively. The half maximal effective concentration(ρ(EC50))of the single and mixed contaminants were calculated. ρ(EC50)values of cartap, Pb and Cr for 15 min tests were 386.23, 5.03, 62.85 mg/L, and that for 30 min tests are 205.48, 4.98, 50.58 mg/L, respectively. Toxicity unit method, mixtures toxicity index method, and additive index method were used to evaluate the joint toxicity of cartap, Pb and Cr on Vibrio fischeri. The results indicated that the cartap-Pb binary system was mainly characterized by synergism, antagonism, and partial additive effects. The ternary combination of cartap-Cr binary and cartap-Pb-Cr were combined, and the main manifestations were synergistic effects. [ABSTRACT FROM AUTHOR]