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旨在为辣木籽抗菌肽的储存、加工提供参考，以实验室前期筛选的具有较好抑制金黄色葡萄球菌生长的辣木籽肽F2和F3为原料，测定其在不同储存、加工条件下的相对抑菌率，分析抑菌稳定性。结果表明：低温储存有利于辣木籽抗菌肽F2和F3抑菌活性的保留，且储存温度为-20 ℃时，其抑菌活性受储存时间影响较小；F2、F3随pH增大相对抑菌率先升高后降低，F2、F3适宜的pH分别为6和8；加工温度、超声波功率升高，F2、F3相对抑菌率降低；相比于Na+和K+，Mg2+对F2、F3相对抑菌率的降低作用更大。实际加工和储存过程中应选择合适的条件，以保证辣木籽抗菌肽抑菌活性的保留。 In order to provide reference for the storage and processing of antimicrobial peptides from Moringa oleifera seeds, the Moringa oleifera seed peptide F2 and F3 with good anti-Staphylococcus aureus activity screened in the laboratory were used as raw materials to determine their relative bacterial inhibition rates under different storage and processing conditions and to analyze antimicrobial stability. The results showed that low temperature storage was beneficial to the rentention of antimicrobial acitivity of F2,F3, and the antimicrobial activity was less affected by the storage time when the storage temperature was -20 ℃. The relative bacterial inhibition rate of F2 and F3 increased and then decreased with the increase of pH, and the suitable pH of F2 and F3 were 6 and 8, respectively. The relative bacterial inhibition rate of F2 and F3 decreased with the increase of processing temperature and ultrasonic power. Compared with Na+ and K+, Mg2+ had a greater effect on reducing the relative bacterial inhibition rate of F2 and F3. The suitable conditions should be selected during actual processing and storage to ensure the retention of the antimicrobial activity of Moringa oleifera seeds antimicrobial peptide.

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This paper summarized the multidisciplinary collaboration-based nursing measuers for a patient undergoing percutaneous transluminal coronary angioplasty (PTCA) combined with cerebrovascular interventional surgery. Key issues and consideration of nursing included: Close observation of vital signs, prevention of cardiac tamponade and intracranial hypertension after interventional surgery, monitoring of kidney function and coagulation function, prevention of arterial puncture bleeding and prevention of venous thrombosis of lower limbs, adopting the treatment of integrated Traditional Chinese and Western medicine to improve the symptoms of constipation and insomnia, improving the postoperative quality of life of patients, comprehensive analysis of patient psychological state after major surgery and implementation of corresponding psychological nursing and specific discharge guidance. (本文总结1例心脏血管经皮冠状动脉腔内成形术(PTCA)合并脑血管介入手术患者的护理措施。护理要点包括: 严密观察生命体征, 介入手术后预防心包填塞与颅内高压, 加强肾脏功能和凝血功能的监测, 预防动脉穿刺处出血和预防下肢静脉血栓形成, 通过给予中西医结合护理改善便秘及失眠的症状, 提高患者术后生活质量, 全面分析患者大手术后心理状态并给予相应的心理护理和有针对性的出院指导。)

为进一步提高辣木籽蛋白资源的开发利用，采用盐提法提取辣木籽蛋白，再采用超声波辅助酶法制备辣木籽ACE抑制肽。以水解度和ACE抑制率为评价指标，通过单因素实验探究超声波功率、超声酶解时间、超声酶解温度及料液比对制备ACE抑制肽的影响,采用响应面法对制备工艺条件进行优化。结果表明：超声波辅助酶法制备辣木籽ACE抑制肽的最佳酶解工艺条件为碱性蛋白酶添加量5.5 mg/mL、pH 9、超声波功率500 W、超声酶解时间1.7 h、超声酶解温度55 ℃、料液比1∶ 45，在此条件下制备的酶解物ACE抑制率达到78.32%，水解度为778%。以辣木籽为原料制备ACE抑制肽作为功能性蛋白肽产品，可有效提高辣木籽蛋白资源的开发利用。 To further improve the exploitation of Moringa oleifera seed protein resources, the protein in Moringa oleifera seeds was extracted by salt extraction method, and then Moringa oleifera seeds ACE inhibitory peptide was prepared by ultrasound-assisted enzymatic hydrolysis. With the degree of hydrolysis and ACE inhibition rate as the evaluation indexes, the effects of the ultrasonic power, ultrasonic enzymolysis time, ultrasonic enzymolysis temperature and ratio of material to liquid on the preparation of ACE inhibitory peptide were studied through single factor experiment. The preparation process conditions of ACE inhibitory peptide were optimized by response surface methodology. The results showed that the optimal ultrasound-assisted enzymatic hydrolysis conditions were obtained as follows: Alkaline protease dosage 5.5 mg/mL,pH 9,ultrasonic power 500 W, ultrasonic enzymolysis time 1.7 h, ultrasonic enzymolysis temperature 55 ℃ and ratio of material to liquid 1∶ 45. Under these conditions, the ACE inhibition rate of the prepared product reached 78.32% and the degree of hydrolysis was 7.78%. Using ACE inhibitory peptide prepared from Moringa oleifera seeds as a functional protein peptide product can effectively improve the exploitation of Moringa oleifera seed protein resources.

Copyright of Journal of South China Agricultural University is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of South China Agricultural University is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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"Godo" is a Japanese fermented soybean food. This study evaluated the effect of temperature on the quality of godo during storage using microbiological methods. The sample stored at 25°C exhibited a thinner odor and white film on its surface. Bacillus subtilis and Wickerhamomyces anomalus were isolated from the sample. When a godo sample containing the isolated strain of W. anomalus was stored at 25°C, it reproduced the thinner odor and white film. The growth of the strain was inhibited at low temperatures, but not by NaCl. These results suggest that W. anomalus is the cause of godo spoilage, and that godo should be stored at low temperatures. [ABSTRACT FROM AUTHOR]

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Online games using Scratch were designed to help students acquire knowledge of molecular biology and biochemistry, which are fundamental to biotechnology. The games were created to enhance understanding of RNA codon tables, the structure of amino acid side chains, the names and structures of organic compounds, the overview of metabolic maps, and English vocabulary related to biotechnology. The students played those games during their classes. The effectiveness of the games was evaluated by a questionnaire. The students rated the games as helpful for their understanding of the classes, whereas the English vocabulary games received lower ratings. Overall, the study showed that the lecturers were able to develop learning games in Scratch and utilize them in a classroom setting even without needing to have any previous programming experience. [ABSTRACT FROM AUTHOR]

Copyright of Nishinihon Journal of Urology is the property of West Japan Urological Association and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Fly Ash Comprehensive Utilization is the property of Hebei Fly Ash Comprehensive Utilization Magazine Co., Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Objective To investigate clinical and epidemiological features of pneumocystis jirovecii pneumonia (PJP) in kidney transplant recipients. Methods Clinical data of 68 kidney transplant recipients admitted from July, 2021 to December, 2021 were collected. All patients were divided into the PJP group (n=11), common pulmonary infection group (n=24) and non-pneumonia group (n=33) according to the status of pulmonary infection. The incidence and treatment of PJP after kidney transplantation were analyzed. Basic characteristics and laboratory parameters of the recipients were compared among all groups. The genotyping and transmission map of PJP patients were evaluated. Results Among 64 kidney transplant recipients, 11 cases were definitely diagnosed with PJP. The most common clinical manifestations included elevated body temperature, and dry cough complicated with progressive dyspnea. Chest CT scan showed diffuse interstitial inflammation and ground glass-like lesions of bilateral lungs in all patients. After diagnosis, all patients were orally given with compound sulfamethoxazole for 3-4 weeks. Two patients received non-invasive ventilator-assisted ventilation due to severe lung infection and dyspnea, and the remaining patients were given with nasal cannula oxygenation. One patient experienced elevated serum creatinine level upon discharge, and developed renal allograft failure. The remaining 10 recipients with PJP obtained normal renal allograft function, and no recipient died. Compared with the non-pneumonia group, the rejection rate was higher, the length of hospital stay was longer, the lymphocyte count was less, the lymphocyte proportion was lower, the levels of C-reactive protein, serum creatinine and lactate dehydrogenase were higher, and the levels of serum albumin was lower and CD4+ T cell count was less in the PJP group (all P<0.05). Compared with common pulmonary infection group, the lymphocyte count was less, the lymphocyte proportion was lower, the CD4+ T cell count was less and 1,3-β-D- glucan (BDG) level was higher in the PJP group (all P<0.05). No new genotype was detected in 10 of the 12 testing samples. It was considered that PJP mainly depended on two transmission chains and two independent transmission individuals. Conclusions Kidney transplant recipients are prone to pneumocystis jirovecii (PJ) infection due to impaired cellular immune function. The most common clinical manifestations consist of elevated body temperature and dry cough complicated with progressive dyspnea, accompanied by headache and fatigue in partial patients. Chest CT scan shows diffuse interstitial inflammation and ground glass-like lesion of bilateral lungs. PJ may be transmitted through respiratory tract. Small-scale PJP might occur in the follow-up outpatient of kidney transplant recipients. Preventive measures should be delivered in a timely manner. [ABSTRACT FROM AUTHOR]

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The choice of running shoes contributes to the running economy （RE）, which is one of the important capacities for distance running performance. Recently, the development of running shoes with high compliance and resilience midsoles and integrated carbon-fiber plates has led to improvements in longdistance running performance. This study aimed to compare the biomechanical variables when wearing carbon-fiber plate running shoes （Nike ZoomX Vaporfly Next%: VN） and conventional running shoes （Nike Zoom Streak 7: ST7） and investigate the factors contributing to the improvement in RE. 10 longdistance runners evaluated the RE, lower limb joint angle, vertical ground reaction force, ground contact time, step frequency, and surface electromyogram with wearing two running shoes （VN and ST7）. Notable results were that 1） the VN was a 5.7 % on average better RE than the ST7, 2） the ankle joint was more in a plantar flexion position throughout the support phase in the VN than the ST7. Other biomechanical variables were not significantly different between the running shoes. In addition, the most RE improvement （17.2 %） runner with the VN compared to the ST7 showed >1SD difference in dorsiflexion of the ankle joint, flexion of the hip joint, and an internal rotation of the ankle joint compared to an average of the ten runner’s lower limb joint angle at the support phase. On the other hand, one runner decreased RE （4.3 %） when wearing the VN compared to the ST7. This runner showed >1SD difference in an internal rotation of the knee joint throughout the support phase compared to an average of the ten runner’s lower limb joint angle. These results suggest that the VN provide superior energy returns, which contributes to the improvement of RE, but not all runners can benefit from the improvement of RE by wearing the VN. [ABSTRACT FROM AUTHOR]

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Copyright of Modern Food Science & Technology is the property of Editorial Office of Modern Food Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)