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Objective To investigate the differences and significance of three ways of removing glands through transurethral enucleation of the prostate （TUREP） in severe benign prostatic hyperplasia patients. Methods 46 cases of TUREP with large gland （over 100g） were selected as the study subjects.TUREP operation was performed in 16 cases，TUREP with tissue morcellator operation was performed in 15 cases, and TUREP with open operation was performed in another 15 cases.The operation time， lithotomy position time， fluid volume of bladder irrigation during operation and estimated volumes of bleeding during operation were recorded and analyzed. Results There were significant differences in operation time and lithotomy position time among this three different operations （P < 0.05） . The fluid volume of bladder irrigation in TUREP with open surgery was significantly less than that in TUREP and TUREP with tissue morcellator operation. Conclusion TUREP with tissue morcellator and TUREP with open surgery can significantly shorten the operation time and the patient's lithotomy position time， and TUREP with open surgery can also reduce the amount of bladder irrigation fluid during the operation. For patients with severe benign prostatic hyperplasia， the operation mode should be reasonably and individually selected according to the basic conditions of the patients. [ABSTRACT FROM AUTHOR]

A near-infrared region (NIR) ratiometric fluorescent probe Cy-P based on hemicyanine was rationally designed, prepared and utilized for the detection of exogenous and endogenous peroxynitrite (ONOO-) in living cells. The research results show that Cy-P itself displays NIR emission owing to the large π-conjugated system. With the addition of the strong oxidizer ONOO-, the π-conjugated system of Cy-P is destroyed, and a new substance with blue fluorescence is formed, leading to 268 nm blueshift of fluorescent emission. The linear detection range of the probe Cy-P to ONOO- is 0-15 μmol/L, and the detection limit is 13 nmol/L. The probe exhibits superior selectivity for ONOO- over various potentially interfering biological analytes. Due to the low cytotoxicity, the prepared probe is suitable for imaging detection of exogenous and endogenous ONOO- in living cells. [ABSTRACT FROM AUTHOR]

This paper introduces the working principle of SLB technology in detail, and offers a scheme to guarantee the reliability of application system and to reduce the working pressure of single server by using its characteristics. It also gives the detailed configuration commands under different working modes. The simulation test proves scheme is effective and practicable. When the servers run normally, the balanced traffic load should be achieved, and the service requests of the users can be answered respectively. Once a server fails, other servers will continue to respond to the users’ request without any interruption. [ABSTRACT FROM AUTHOR]

Allogeneic hematopoietic stem cell transplantation (Allo-HSCT) is an effective therapy for acute leukemia. But some patients with relapse after transplantation lead to treatment failure. A few patients who have extramedullary relapse after transplantation. The treatment outcome is limited and the overall prognosis is poor. Purely in patients with mammary gland relapse is rare. One case of mammary gland relapse with acute myeloid leukemia after bone marrow transplantation is reported to to increase the understanding of such patients. [ABSTRACT FROM AUTHOR]

Gravity anomaly inversion is commonly used as a way to explore the earth. The density of underground materials can be inferred by observing gravity anomalies on the surface. The following problems are commonly addressed in the traditional gravity anomaly inversion methods: diversification, initial model dependence, and long calculation time. A gravity anomaly inversion method based on a convolutional neural network (CNN) was proposed, the dataset was obtained through numerous forward calculations. Then the data set was applied to train the CNN network. Thereby, the mapping relationship would be established between the gravity anomaly observed on the surface and the density distribution of the underground medium. Finally, the gravity anomaly data was put into the trained convolutional neural network. Through this, the corresponding underground Medium density distribution was obtained. The following results can be observed in this experiment: the density, location, and boundary of underground gravity anomalies are quickly and accurately inversed by this method. A strong generalization ability and effective problem solving for gravity anomaly inversion are also indicated by this experiment. [ABSTRACT FROM AUTHOR]

Owing to the light weight high durability and low costs plastics are ubiquitous in consumer goods worldwide. However with a rapid generation rate and a low recycling rate most plastics are accumulated in landfills and natural environments. In particular polyethylene terephthalate PET is widely used for fiber production in the textile sector. Besides PET suspensions from industrial production waste streams and microplastics from the decomposition of PET waste in the environment have gently entered the human food system. For instance seafood tea and vegetables have posed nonnegligible threat to food safety and agricultural sustainability. What' s worse plastic polymers have been detected in the blood of humans in the latest study in 2022. Unfortunately some common chemical processes for PET degradation such as hydrolysis methanolysis and glycolysis require the use of extensive solvent and degradation agents the reaction pressure is high and oligomeric side products emerging after these processes are often laborious to separate. Recently the utilization of solar light reforming technology has emerged as an attractive strategy to deconstruct PET. To solve the ecological pollution problem of non-degradable PET TiO2 / Pt photocatalysts CdS / CdOx quantum dots and CNx / Ni2 P photocatalysts have been used to convert waste PET into valuable chemicals while hydrolyzing to produce H2 under the driving of sunlight. However these catalysts have defects such as high price toxicity difficulty in recycling and secondary pollution. Therefore in this paper electrostatically spun nanofiber films are used as the substrate. Cheap environmentally friendly efficient and stable and recyclable polycrystalline nickel phosphide / titanium dioxide / carbon Ni2 P-NiP2 / TiO2 / C nanofiber film photocatalysts are prepared by heat treatment hydrothermal method and phosphate treatment. It is shown that the unique fiber structure of the electrostatically spun nanofiber film provides excellent flexibility for the catalyst and also provides a high-quality carrier for polycrystalline nickel phosphide. In addition the photocatalytic system successfully degrades PET fibers into organic small molecules under alkaline aqueous solution conditions while hydrolyzing to produce H2 completing the resource reuse of waste PET. Moreover the catalyst can be easily separated from the solution and recycled and the efficient hydrogen precipitation performance can be maintained after several cycles. The electrostatic spinning nanofiber photocatalysts supply technical support for the reuse of waste PET fibers and also furnish direction and reference for exploring sixpenny green and recyclable photocatalysts. However there is still potential for further improvement in solar light utilization as well as alkali resistance. [ABSTRACT FROM AUTHOR]

Allogeneic hematopoietic stem cell transplantation (Allo-HSCT) is an effective therapy for acute leukemia. But some patients with relapse after transplantation lead to treatment failure. A few patients who have extramedullary relapse after transplantation. The treatment outcome is limited and the overall prognosis is poor. Purely in patients with mammary gland relapse is rare. One case of mammary gland relapse with acute myeloid leukemia after bone marrow transplantation is reported to to increase the understanding of such patients. [ABSTRACT FROM AUTHOR]

Objective: To explore the value of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) combined with serum tumor supplied group of factors (TSGF), type Ⅰ collagen carboxyl pyridine terminal peptide (ICTP) and alkaline phosphatase (ALP) in the identification of benign and malignant bone tumors and the efficacy evaluation of bone malignant tumors after treatment.Methods: 163 patients with bone tumors who were admitted to our hospital from December 2020 to April 2022 were selected as research subjects, and patients were divided into benign group (74 cases) and malignant group (89 cases) according to the benign and malignant bone tumors. Malignant group was divided into good efficacy group and poor efficacy group according to the evaluation criteria of solid tumor efficacy after treatment. The volume transfer constant (Ktrans), rate constant (Kep), extravascular extracellular space volume fraction (Ve), area under initial curve within 1 minute (i AUC), TSGF, ICTP and ALP were compared between benign group and malignant group, good efficacy group and poor efficacy group. The correlation between Ktrans, Kep, Ve, i AUC and serum TSGF, ICTP,ALP was analyzed. The predictive value of each index for benign and malignant bone tumors were analyzed by receiver operating characteristic (ROC) curve. Results: Ktrans, Kep, i AUC, TSGF, ICTP and ALP were compared between different groups, benign group was lower than that of malignant group (P＜0.05), good efficacy group was lower than that of poor efficacy group (P＜0.05) ; Ktrans, Kep and i AUC in DCE-MRI quantitative parameters were positively correlated with serum TSGF, ICTP and ALP (P＜0.05) . The area under the curve (AUC) of Ktrans, Kep, i AUC, TSGF, ICTP, ALP and combined prediction of benign and malignant bone tumors were 0.794,0.804, 0.744, 0.747, 0.773, 0.828 and 0.986 respectively, and the AUC of the combined prediction of benign and malignant bone tumors was greater than that of each index alone. Conclusion: DCE-MRI quantitative parameters Ktrans, Kep, i AUC combine with serum TSGF,ICTP and ALP have high value in differentiating benign and malignant bone tumors, the higher the Ktrans, Kep, i AUC, TSGF, ICTP, and ALP, the worse the clinical efficacy of patients with malignant bone tumors after treatment. [ABSTRACT FROM AUTHOR]

Objective To explore the clinical value of audio-visual response and CNV changes in children with attention deficit hyperactivity disorder (ADHD) . Methods A total of 90 ADHD children aged 7-12 years were selected and 30 healthy children were selected as controls. The Swanson, Nolan and Pelham-IV rating scales (SNAP-IV scale),the integrated visual and auditory continuous performance test (IVA-CPT) and CNV test were performed respectively. The X2 test and independent samples t test were used to verify the regularity of cognitive function in children with ADHD. Results (1) The difference of SNAP-IV scores between the two groups was statistically significant (P<0. 05) . (2) The differences of synthesis, audiovisual attention quotient and control quotient in IVA-CPT between the two groups were statistically significant (P<0. 05),and there were statistically significant differences in the ability of audio-visual attention concentration and retention between the two groups in undertaking assignment tasks (P<0. 05) . (3) There was no significant difference of CNV in the PINV latency between the two groups (P>0. 05) . In terms of PINV amplitude, ADHD group was significantly lower than control group and the difference was statistically significant (P<0. 05) . Conclusion (1) The performance of ADHD patients′ attention deficit/hyperactivity significantly affects their daily activities, and it is obvious to their parents. (2) The degree of concentration and retention of audio-visual attention and retention of ADHD patients in completing daily activities and learning-related homework tasks are significantly reduced. (3) The difference of CNV test in ADHD patients is mainly reflected in the amplitude. [ABSTRACT FROM AUTHOR]

To investigate the difference in protein expression between hepatocellular carcinoma ( HCC) patients with recurrence and those with good prognosis, the differential expression and regulatory mechanism of miR - 152 - 3 p target proteins, and the role of miR - 152 - 3p in the recurrence of HCC. Methods TMT - labeled proteomic sequencing and RT - PCR were used to measure the expression of proteins and the expression of miR - 152 - 3 p in the HCC tissue of six patients with recurrence at 2 years after HCC resection and six patients with good prognosis at 5 years. Six databases were used to analyze the target genes of miR - 152 - 3p, and Gene Ontology, DAVID, and REACTOME databas es were used to perform target gene screening, enrichment annotation, and signal transduction pathway enrichment analysis. Gene mutation frequency and survival curve analysis were performed for the target genes of miR - 152 - 3p to verify the role of miR - 152 - 3 p target genes in patients with HCC recurrence. The independent samples t - test was used for comparison of continuous data between two groups, and a Kaplan - Meier analysis was performed to investigate the survival rates of liver - related genes. Results Compared with the patients with HCC recurrence, the patients with good prognosis after HCC resection had a significantly higher transcriptional expression level of miR - 152 - 3 p in HCC tissue ( P < 0. 05). The results of protein sequencing showed that there were 365 differentially expressed proteins in HCC tissue between the patients with good prognosis and the patients with recurrence, and the analysis of HCC recurrence databases showed that 17 proteins were regulated by miR - 152 - 3 p. Further analysis of the signaling pathways showed that the function of the 17 target genes regulated by miR -152 - 3p was enriched in the translation and regulation of mitochondria and ribosome, and multiple enrichment revealed that six target genes were closely associated with mitochondrial respiratory chain complex, i. e ., AKAPl, FOXREDl, MRPL28, MRPL50, SHCl, and STAUl. Gene mutation frequency and survival curve analysis showed that the loss or weakening of the function of mitochondrial respiratory chain - related target proteins seriously affected the prognosis and survival rate of patients. Conclusion There is a significant difference in the expression of miR - 152 - 3p in HCC tissue between patients with good prognosis and those with recurrence after HCC resection, and miR - 152 - 3 p may lead to the recurrence of HCC by regulating the target genes AKAPl, FOXREDl, MRP128, MRPL50, SH Cl, and STA Ul, acting on the mitochondrial respiratory chain, and affecting the oxidative respiratory function of cells. [ABSTRACT FROM AUTHOR]

ObjectiveTo investigate the mechanism of HCV infection in the pathogenesis of atherosclerosis with a model of human umbilical vein endothelial cells (HUVECs) stimulated by HCV in vitro. MethodsHUVECs were stimulated with 1.0 MOI HCVcc for 48 hours. CCK8 assay was used to measure cell proliferation; flow cytometry was used to measure cell apoptosis and cell cycle; wound healing assay and monocyte-endothelial adhesion assay were used to evaluate the influence of HCV on the migration and adhesion of HUVECs; quantitative real-time PCR and Western blot were used to measure the expression of inflammatory factors and endothelial injury factors in HUVECs stimulated by HCV. The two-independent-samples t test was used for comparison between two groups; an analysis of variance was used for comparison between multiple groups, and the LSD-t test was used for further comparison between two groups. ResultsCompared with the control group, the HCV group had no significant changes in the growth, apoptosis, and cell cycle of HUVECs (all P＞0.05). HCV stimulation inhibited the migration of HUVECs and enhanced their adhesion ability. Compared with the control group, the HCV group had significant increases in the mRNA and protein expression of the inflammatory factors interleukin-6 and interleukin-1β and the chemokines CXCL10 and monocyte chemotactic protein 1 (mRNA expression: t=-10.155, -12.048, -5.025, and -20.116, all P＜0.05; protein expression: F=2541.739, 4806.490, 477.608, and 501.38, all P＜0.001). HCV stimulation significantly upregulated the expression of the endothelial injury factors endothelin-1 and vascular endothelial growth factor and the adhesion molecules intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 in HUVECs (t=-4.530, -4.497, -7.692, and -7.449, all P＜0.05). ConclusionHCV can cause inflammatory changes and dysfunction in endothelial cells and thus affect the development of atherosclerosis. [ABSTRACT FROM AUTHOR]

BACKGROUND: The higher thermal maturity of organic matter and the shortage of terrestrial plants are the main characteristics of Lower Paleozoic marine shale in China, which results in the difficulty of distinguishing macerals. Nowadays the existing methods of vitrinite reflectance, rock eval, Tm/Ts and other biomarker indices cannot be effectively used to evaluate the maturity of organic matter in shale. OBJECTIVES: To evaluate the maturity of organic matter in highly evolved shale. METHODS: The shale of the Silurian Longmaxi Formation of Well Anye-1 in Zheng'an area of Guizhou Province is used in the experiments. Field Emission Scanning Electron Microscopy and Laser Raman Spectroscopy are used to obtain the Raman Spectra of organic matter in different occurrences of highly evolved shale, independent of the type of organic macerals. RESULTS: It can be found that there are four types of organic matter in the Silurian Longmaxi Formation of Well Anye-1, i.e. bio-structural type, vein-like or clump-like type, euhedral and anhedral boundary filling gap shape. The euhdedral boundary filling gap shape has an organic aperture larger than 80nm, whereas the anhedral boundary filling gap shape has an organic aperture less than 60nm. These two types of organic matter are the main reservoir space of rock reservoirs, which account for more than 90%. Four types of organic matter have different thermal evolution degrees. Moreover, the Raman maturity ranges from 2.8% to 3.0%, which is calculated from the laser Raman surface scanning data. The proportion of Raman maturity of various organic matters to the total amount is consistent with the semi-quantitative statistical results observed by Field Emission Scanning Electron Microscopy. CONCLUSIONS: The methods used in the experiments are effective in measuring the maturity of organic matter in highly evolved marine shale in China. [ABSTRACT FROM AUTHOR]

Objective To explore the level of β2-MG and Cys-C in renal injury after interventional therapy and the significance of these changes．Methods The levels of β2-MG and Cys-C in 44 cases of renal injury treated with interventional therapy and 40 normal cases were dynamically tested and compared．Results The levels of β2-MG in the observation group were significantly higher than those in the normal control group one week after operation and one month after operation（ ＜0.05），and there were no significant differences in β2-MG levels between 2 months after operation and 3 months after operation （ ＞0.05） ．The serumlevels of Cys-C in the observation group were significantly higher than those in the normal control group both before and one week after interventional therapy， and the differences were statistically significant（ ＜0.05） . After interventional therapy， the serum Cys-C level in the observation group decreased gradually. There was no significant difference in serum Cys-C levels between the two groups 1 month， 2 months and 3 months after operation （ ＞0.05） ．Conclusion After renal injury， the levels of β2-MG and Cys-C are higher than those of the control group. （ < 0.05），which are indicators of the renal injury changes. After interventional embolization，the levels of β2-MG and Cys-C decreased gradually.The detection and analysis of β2-MG and Cys-C suggest that renal function can recover effectively after interventional therapy. [ABSTRACT FROM AUTHOR]

BACKGROUND: AnYe -1 well is a geological survey parameter well implemented by China Geological Survey. It has achieved shale gas breakthrough in the Willing complex structural area outside the southern basin of China. OBJECTIVES; To quantitatively characterize micro- and nano-pore structure and development characteristics of organic matter, by researching organic matter pore on Longmaxi shale of AnYe - 1 well. METHODS; Field Emission-Scanning Electron Microscope was used to study the reservoir type of the organic-rich shale of the Longmaxi Formation at the periphery of the basin in the Upper Yangtze region, and the PerGeos digital rock treatment system was introduced into the organic pores quantitative analysis. RESULTS; The organic pore is the most important reservoir space of the organic-rich shale of the Longmaxi Formation in Anye - 1 well. It is characterized by organic matter interstitially forming in the authigenic siliceous organic matter with uniform sponge-like pore structure, or organic matter that interacts with clay minerals with a bubble-like pore structure. Pores in two different types of organic matter are extremely developed. The PerGeos digital rock system reveals that the pore sizes of the two organic pores show a himodal distribution, in which the pore size of the organic pores with the sponge-like structure is 5 to 10 nm. and the pore size of the organic pores with the bubble-like structure is 51 to 100 nm. The organic pores are mainly in the mesopore to macropore category. CONCLUSIONS: The abundant development of organic matter pores in the Longmaxi Formation in AnYe - 1 well indicates that the Longmaxi Formation shale in the periphery of the basin has experienced a strong hydrocarbon generation process and has a good storage capacity, showing a potential for development in the future. [ABSTRACT FROM AUTHOR]