miR-152-3p 靶蛋白差异表达及调控机制在肝癌复发中的作用.

To investigate the difference in protein expression between hepatocellular carcinoma ( HCC) patients with recurrence and those with good prognosis, the differential expression and regulatory mechanism of miR - 152 - 3 p target proteins, and the role of miR - 152 - 3p in the recurrence of HCC. Methods TMT - labeled proteomic sequencing and RT - PCR were used to measure the expression of proteins and the expression of miR - 152 - 3 p in the HCC tissue of six patients with recurrence at 2 years after HCC resection and six patients with good prognosis at 5 years. Six databases were used to analyze the target genes of miR - 152 - 3p, and Gene Ontology, DAVID, and REACTOME databas es were used to perform target gene screening, enrichment annotation, and signal transduction pathway enrichment analysis. Gene mutation frequency and survival curve analysis were performed for the target genes of miR - 152 - 3p to verify the role of miR - 152 - 3 p target genes in patients with HCC recurrence. The independent samples t - test was used for comparison of continuous data between two groups, and a Kaplan - Meier analysis was performed to investigate the survival rates of liver - related genes. Results Compared with the patients with HCC recurrence, the patients with good prognosis after HCC resection had a significantly higher transcriptional expression level of miR - 152 - 3 p in HCC tissue ( P < 0. 05). The results of protein sequencing showed that there were 365 differentially expressed proteins in HCC tissue between the patients with good prognosis and the patients with recurrence, and the analysis of HCC recurrence databases showed that 17 proteins were regulated by miR - 152 - 3 p. Further analysis of the signaling pathways showed that the function of the 17 target genes regulated by miR -152 - 3p was enriched in the translation and regulation of mitochondria and ribosome, and multiple enrichment revealed that six target genes were closely associated with mitochondrial respiratory chain complex, i. e ., AKAPl, FOXREDl, MRPL28, MRPL50, SHCl, and STAUl. Gene mutation frequency and survival curve analysis showed that the loss or weakening of the function of mitochondrial respiratory chain - related target proteins seriously affected the prognosis and survival rate of patients. Conclusion There is a significant difference in the expression of miR - 152 - 3p in HCC tissue between patients with good prognosis and those with recurrence after HCC resection, and miR - 152 - 3 p may lead to the recurrence of HCC by regulating the target genes AKAPl, FOXREDl, MRP128, MRPL50, SH Cl, and STA Ul, acting on the mitochondrial respiratory chain, and affecting the oxidative respiratory function of cells. [ABSTRACT FROM AUTHOR]