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Copyright of Journal of International Reproductive Health/Family Planning is the property of Editorial Board of the Journal of International Reproductive Health/Family Planning and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To explore the potential mechanism of Schisandrae chinensis fructus on the treatment of missed abortion based on a network pharmacology approach and verified experimentally. Methods: The system pharmacology approach was used to find and screen the active components and targets of Schisandrae chinensis from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP, https://old.tcmsp-e.com/tcmsp.php), find the genes related to missed abortion, and identify the targets of Schisandra chinensis for prevention and treatment of missed abortion. The "drug component-target" network was constructed by using Cytoscape to screen key compounds. The protein-protein interaction (PPI) network was established by String, and the core targets were screened by the Cytoscape -CytoNCA topology analysis. Gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) analysis were performed on the target genes to predict their possible signal pathways and explore their molecular mechanisms. Finally, the mechanism was validated with the help of human chorionic villous trophoblast cell line (HTR-8/SVneo) using real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) detection and Western blotting assays. Results: Seven active compounds of Schisandra chinensis were identified from TCMSP database. Schisandrae chinensis has five potential targets for the prevention and treatment of missed abortion. The PPI network shows that androgen receptor (AR), estrogen receptor (ER), cyclooxygenase -2 (COX -2), nuclear receptor coactivator 2 (NCOA2) and acetylcholine esterase (ACHE) may be the core targets of Schisandrae chinensis for the prevention and treatment of missed abortion. Forty-four cell biological processes were obtained by GO enrichment analysis, and two related signal pathways were obtained by KEGG pathway enrichment analysis, mainly including thyroid hormone signal pathway and estrogen signal pathway. During the process of validating the mechanism using a cell model, it was found that the protein and/or mRNA levels of AR, ER, and COX-2 in cells treated with benzo(a)pyren-7, 8-dihydrodiol-9, 10-epoxide (BPDE) were higher than those of normal cells, while the protein and/or mRNA levels of AR, ER, and COX-2 were reduced in cells pretreated with Schisandrin B (Sch B) first and then tainted with BPDE compared with those not pretreated with Sch B. The NCOA2 and ACHE protein and/or mRNA levels did not change significantly. Conclusions: Schisandrae chinensis may exert its preventive and therapeutic effects on missed abortion through anti-inflammatory and hormonal receptor regulation, and this protective effect may be achieved through the estrogen signaling pathway. [ABSTRACT FROM AUTHOR]

Copyright of Occupational Health & Emergency Rescue is the property of Occupational Health & Emergency Rescue Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Kunming Medical University / Kunming Yike Daxue Xuebao is the property of Kunming Medical Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Architecture & Civil Engineering is the property of Chang'an Daxue Zazhishe and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

To observe the application value of Azithromycin combined with Danhuang Quyu capsule in patients with chronic pelvic inflammatory disease. 127 patients with chronic pelvic inflammatory disease who were treated in our hospital from June 2019 to June 2021 were selected, they were randomly divided into control group (63 cases, azithromycin treatment) and observation group (64 cases, Danhuang Quyu capsule combined with azithromycin treatment), both groups were treated for 2 weeks. The curative effect, disappearance time of clinical symptoms, hemorheology and changes of serum inflammatory factors were compared between the two groups. The occurrence of adverse reactions in the two groups were observed and recorded. The total effective rate of the observation group was higher than that of the control group (P<0.05). The disappearance time of lumbosacral pain, lower abdominal pain and abnormal band in the observation group were shorter than those in the control group (P<0.05). The levels ofinterleukin-6 (IL-6), interleukin-1 β（IL-1 β）, C-reactive protein (CRP) in the observation group at 2 weeks after treatment were lower than those in the control group (P<0.05). The whole blood high shear viscosity, whole blood low shear viscosity, fibrinogen and erythrocyte sedimentation rate in the observation group at 2 weeks after treatment were lower than those in the control group(P<0.05).There was no significant difference in the incidence of adverse reactions between the two groups (P>0.05). On the basis of azithromycin treatment, combined with Danhuang Quyu capsule, the symptoms of chronic pelvic inflammatory disease can be significantly alleviated. At the same time, it can also improve the level of Hemorheology and inflammatory factors. It has good clinical application value. [ABSTRACT FROM AUTHOR]

Objective: To investigate the relationship between serum homocysteine (Hcy) level and renal function and carotid atherosclerosis in patients with type 2 diabetes mellitus (T2DM) complicated with hypertension. Methods: The clinical data of 168 patients with T2DM who were admitted to Anhui Second People's Hospital from July 2018 to May 2021 were retrospectively analyzed. According to whether they were complicated with hypertension or not, they were divided into T2DM complicated with hypertension group (combined group) 87 cases and simple T2DM group (T2DM group) 81 cases, another 87 healthy subjects were selected as the control group during the same period. The serum Hcy level and renal function indexes [serum creatinine(Scr), urine creatinine(CR), urine microalbumin/creatinine(ACR), urine immunoglobulin G/creatinine (IGU/CR), urine transferrin/creatinine (TRU/CR), urine α1-microglobulin/creatinine(α1/CR), Glomerular filtration rate (eGFR)] and left and right carotid intima-media thickness (IMT), Crouse integral of each group were compared. The correlation between Hcy level and renal function indexes, IMT, Crouse integral were analyzed by Pearson correlation analysis. Results: The level of the serum Hcy, Scr, CR, ACR, IGU/CR, TRU/CR, α1/CR and left IMT, right IMT, Crouse integral in the combined group and T2DM group were higher than those in the control group, and those in the combined group were higher than those in T2DM group (P<0.05). The level of eGFR in the combined group and T2DM group was lower than that in the control group, and that in the combined group was lower than that in the T2DM group (P<0.05). Pearson correlation analysis showed that the serum Hcy level was positively correlated with Scr, CR, ACR, IGU/CR, TRU/CR, α1/CR, left IMT, right IMT and Crouse integral in T2DM patients with hypertension (P<0.05), and negatively correlated with eGFR level (P<0.05). Conclusion: The serum Hcy level in T2DM patients with hypertension is abnormally increased, which is related to renal function injury and carotid atherosclerosis. [ABSTRACT FROM AUTHOR]

Objective To observe the clinical effect of vital pulp therapy in permanent molars with cariously exposed pulp that can not complete root canal therapy. Methods Thirty patients with carious exposed pulp permanent molars who could not compete root canal therapy were treated by partial pulpotomy (12 cases) or pulpotomy (18 cases). The bioceramic material iRoot BP Plus was used to preserve the pulp. The patients were followed up regularly after operation, and the treatment effect was observed by clinical and imaging examination. Results After 12-36 month follow-up, 26 cases showed a good clinical effect, no pain and the root and periapical were normal. Two cases had cold stimulation sensitivity after operation and developed to acute pulpitis. Two cases had buccal sinus. The pulp vitality test showed that the pulp was dead and the teeth were extracted. Conclusion The vital pulp therapy in permanent molar with cariously exposed pulp has good clinical effect. The operation of vital pulp therapy is easier and the treatment time was shorter than that of root canal therapy. [ABSTRACT FROM AUTHOR]

Objective: To explore the feasibility and acceptability of the online eating disorder care skills training in China. Methods: Totally 84 caregivers of eating disorder patients who met DSM-5 diagnostic criteria were invited to participate skills lectures and group discussion interventions. The Eating Disorders Symptom Impact Scale(EDSIS) and General Health Questionnaire-12(GHQ12) were undertaken at baseline, at the end of the six-week intervention and after 3 months. At the end of the training, the training satisfaction questionnaire was added. Results: Compared with baseline, at the end of the intervention and after 3 months the scores of EDSIS-guilt and EDSIS-dysregulated behavior decreased, the scores of EDSIS-nutrition increased, and the scores of GHQ-12 decreased(Ps <0. 05). The caregivers came from all over the country, 92. 8% of them participated in the training more than 75%, and 89. 3% of them were satisfied with the effectiveness of skill training with 10 points. Conclusion:The online skills training has good feasibility and acceptability in China. [ABSTRACT FROM AUTHOR]

Objective：To explore the cytotoxicity of dihydrodiol benzobenzopyrene [7, 8 dihydrodiol 9,10 epoxidebenzo(a) pyrene , BPDE], a metabolite of Benzo(a) pyrene (BaP), in human choriotrophoblastic cell line (HTR-8/SVneo cell). The protective effect of Schisandrin B (Sch B) on the cell injury induced by BPDE was also investigated. Methods: HTR-8/SVneo cells were used to construct a model of BPDE cytotoxity and a model of Sch B protection. The cell proliferation was tested by MTS in two groups: one group was treated by different concentrations of BPDE alone; another was treated by Sch B combined with BPDE administration. Results: 5 μmol/L of BPDE significantly reduced the cell viability, and the number of living cells was only 38.23% of the total cells. The cells were pretreated with different concentrations of Schisandrin B (0.625, 1.25, 2.5, 5, 10 and 20 μmol/L), and followed by BPDE. The cell viability increased significantly (40.11%, 42.68%, 46.47%, 47.34%, 61.37% and 49.87%, respectively), which suggested a dose-dependent manner of protective effect of Sch B from 0.625 μmol/L to 10 μmol/L. Conclusions: BPDE has a toxic effect on HTR-8/SVneo cells in a dose-dependent manner; while Sch B (0.625-10 μ mol/L) has a protective effect on the cell injury induced by BPDE. [ABSTRACT FROM AUTHOR]

Objective：To establish a high performance liquid chromatography with fluorescence detection (HPLC-FLD) for the detection of Benzo(a)pyrene in the embryonic tissues of rat early pregnancy, so as to provide experimental data for future clinical application. Methods：The level of Benzo(a)pyrene in the embryonic tissues of rats was determined by Shimadzu HPLC with Shimadzu RF -535 fluorescence detector. The samples were extracted by acetonitrile, without cleaned-up. The chromatographic separation was performed using a column of Kromasil C18 (250 mm×4.6 mm, 5 μL) with a mobile phase consisting of methanol and water (95∶5, V/V) at the flow rate of 1.0 mL/min. Excitation wavelength (Ex) of fluorescence detection was 364 nm, the emission wavelength (Em) 427 nm, and the injection volume 20 μL. Results：The level of Benzo (a)pyrene in the tissues of rats was linear in the range of 0.002 μg/mL to 0.5 μg/mL. The regression equation of standard curve of extraction was Y=426 174x－ 316.95 with a correlation coefficient of 0.999 9. The average recovery for Benzo(a)pyrene standard spiked was more than 95% while the inter-day precision RSD was 3.38%, the intra-day precision RSD was 2.80%, and the stability (n=6) was 2.58%. The benzo(a)pyrene concentration of samples was in the range of 0-0.5837 μg/g. Conclusions： The HPLC-FLD method has the advantages of simple operation, low experiment cost, and coincident veracityand sensitivity. The process of sample extraction is simple and time-saving. We conclude that the HPLC-FLD may be a suitable way for the detection of benzo(a)pyrene in samples such as embryonic tissues in the clinical practice and research projects. [ABSTRACT FROM AUTHOR]

Objective：To study the effect of Benzo[a]pyrene (BaP) on the early pregnancy and its mechanism, as well as the protective effect of Schisandra chinesis extrac (SCE) on the Bap-induced damage of early embryo. Methods: Female SD rats were randomly divided into 5 groups (15 rats/group): control group, model (BaP 2 mg· kg-1·d-1) group, low-dose SCE (BaP plus SCE 40 mg·kg-1·d-1) group, middle-dose SCE (BaP plus SCE 200 mg· kg-1·d-1) group and high-dose SCE (BaP plus SCE 1 000 mg·kg-1·d-1) group. After 15 days of treatment, those female rats were mated with normal male rats to get the pregnancy. On the ninth day of gestation, all female rats were sacrificed. The ultrastructural change of embryo was observed by transmission electron microscope. The levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) in the embryonic tissues were measured by the oxidant-injury testing Kits; the level of 8-hydroxy-2 deoxyguanosine (8-OhdG) was also measured by a ELISA Kit. Results: There were no obvious abnormalities on the surface and ultrastructure in the control group and three SCE groups. The embryos in the model group were of uneven size and abnormal blood stasis on surface and abnormal ultrastructure, as well as abnormal apoptosis. The levels of SOD, GSH-Px were significantly decreased in the model group when compared with the control group (P＜0.05 or P＜0.01), while the level of MDA was increased significantly (P＜0.01). The levels of SOD in three SCE groups were significantly increased when compared with the model group (P＜0.01 or P＜0.05). The level of GSH-Px was significantly increased, and the level of MDA decreased in the high-does SCE group when compared with the model group (both P＜0.05). Compared with the control group, the model group had significantly lower level of 8-OHdG (P＜0.01). Interestingly, the levels of MDA were significantly decreased in the high-dose and middle-does SCE groups when compared with the model group (P＜0.01 or P＜0.05). Conclusions: Benzo [a] pyrene exposure before pregnancy has the embryonic toxicity in rats, including oxidative damage, DNA damage and inducted apoptosis. SCE play a protective role in the early embryo through antioxidant, anti-DNA damage and anti-apoptosis. [ABSTRACT FROM AUTHOR]

Copyright of Tianjin Medical Journal is the property of Tianjin Medical Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: Using human trophoblast HTR-8/SVneo (HTR) cell lines for the study of the carrier to study Schisandrin B (Sch B)′s action of benzo[a]pyrene (BaP) damage effect on HTR cell lines aggressivity. Methods:Experiment is divided into the control group, the BaP infected group, different dose Sch B (0.5, 1, 2 μmol/L), intervention infected groupcell proliferation by MTS experimental detection, clear BaP canister and Sch B intervention dose, by Transwell invasion experiment after the detection of BaP infected cells and Sch B cell invasion force change after intervention. Results: ①The MTS test cell proliferation, compared with control group, 20 μmol/L BaP concentration cell proliferation significantly decreased after effect (P<0.01), after Sch B (0.5, 1, 2 μmol/L) intervention the cell proliferation is obviously better than BaP infected group (P< 0.05). ②Transwell cell invasion experiment, compared with control group, the BaP cells infected group was much less aggressive (P<0.05). Sch B intervention group cell invasion force also declined (P<0.05). Compared with the infected group, Sch B cells of aggressive intervention group was obviously ascending (P<0.05). Conclusions:①A certain concentrations of BaP damaged HTR cell proliferation and invasion force. ②Sch B can prevent the damage of BaP of HTR cell proliferation, and at the same time improve cell aggressivity. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of International Obstetrics & Gynecology is the property of TianJin Medical Information Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)