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BACKGROUND: Disturbances in bone metabolism have a significant association with ferroptosis in steroid-induced osteonecrosis of the femoral head (SONFH). Furthermore, the pathologic process of SONFH is characterized by the presence of cartilage damage and degeneration. However, the specific regulatory targets and the relationship between ferroptosis and cartilage concerning SONFH remain unclear. OBJECTIVE: To employ bioinformatics and machine learning techniques to identify specific genes associated with ferroptosis that target cartilage and to investigate the correlation between ferroptosis and cartilage, thereby providing novel ideas and methodologies for the study and treatment of SONFH. METHODS: Disease datasets pertinent to the study and ferroptosis-related genes were retrieved from the GEO and FerrDb databases. Subsequently, the disease datasets were normalized and differential analysis using the R language to identify ferroptosis-related differential genes (Fe-DEGs). We conducted Gene Ontology (GO) functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathway enrichment analysis of Fe-DEGs. Furthermore, ferroptosis-related signature genes were filtered based on the protein-protein interaction network of Fe-DEGs and machine learning methods. Finally, the rabbits were divided into normal and model groups. The normal group was given the same dose of saline to simulate the modeling drug, and the animal model of SONFH in rabbits was constructed by injection of modified horse serum combined with methylprednisolone. After successful modeling, the expression of signature gene was verified between different groups, and the phenotype of ferroptosis in cartilage was analyzed. RESULTS AND CONCLUSION: Through the normalization and differential analysis of the dataset, a total of 1 315 differentially expressed genes were identified. Additionally, 379 ferroptosis-related genes were obtained from the FerrDb database. After intersecting both gene sets, 19 Fe-DEGs were obtained. The GO analysis revealed that Fe-DEGs were mainly involved in biological processes such as cell migration and cellular response to oxidative stress, cellular components such as kinase complexes, amino acid complexes, and cytoplasmic membranes, as well as molecular functions such as kinase activity, receptor activity, and protein binding. The KEGG analysis revealed that Fe-DEGs were mainly enriched in the FoxO signaling pathway, vascular endothelial growth factor signaling pathway, and FcγR-mediated phagocytosis. Constructing a protein-protein interaction network and using machine learning, we identified the ferroptosis-related signature gene, CA9. The gene set enrichment analysis of the signature gene CA9 revealed an upregulated expression in biological processes such as fatty acid metabolism and O-GlcNAc glycosylation modification, while being inhibited in terms of neural activity and ligand-receptor interactions. RT-PCR and western blot results showed that compared with the normal group, the expressions of ACSL4 and CA9 at mRNA and protein levels were significantly higher in the model group (P < 0.05), while the expressions of SLC7A11 and GPX4 at mRNA and protein levels were significantly lower in the model group (P < 0.05), coinciding with the expression levels of the signature genes in the dataset. These findings indicate that the cartilage of SONFH is closely related to ferroptosis, and targeting the signature gene may provide certain ideas and directions for the study and treatment of SONFH. [ABSTRACT FROM AUTHOR]

目的探讨腹腔镜子宫肌瘤剥除术对子宫肌瘤患者术后生活质量及复发率的影响。方法选取该院 收治的子宫肌瘤患者88 例，按随机数字表法分为对照组和观察组，对照组行常规开腹手术，观察组行腹腔镜子宫肌瘤 剥除术，采用视觉模拟疼痛量表（VAS）及简易健康状况量表（SF-36）对两组疼痛程度及生活质量进行评分，并观察两组 手术时间、术中出血量、术后排气时间及住院时间，术后1 年随访两组妊娠率及复发率。结果治疗后，观察组VAS 评 分低于对照组，差异有统计学意义（P<0.05）；治疗后，观察组SF-36 评分高于对照组，差异有统计学意义（P<0.05）；观察 组手术时间、术后排气时间、住院时间及术中出血量优于对照组，差异有统计学意义（P<0.05）；术后随访1 年，观察组妊 娠率高于对照组，差异有统计学意义（P<0.05）；观察组复发率低于对照组，差异有统计学意义（P<0.05）。结论采用腹 腔镜子宫肌瘤剥除术治疗子宫肌瘤能有效减少术中出血量，缓解患者疼痛程度，缩短手术时间、术后排气时间及住院时 间，降低复发率，提高患者生活质量和术后妊娠率。 [ABSTRACT FROM AUTHOR]

The purpose of the experiment was to study the main nutrient contents of straw and blighted husk roughage, and to provide theoretical basis for the efficient utilization of roughage. The results showed as follows: The order for feeding value of 8 kinds of roughage were soybean hull, peanut straw, wheat hull, rice straw, wheat straw, soybean straw, rice hull, peanut hull, and the feeding value were 159.43, 133.82, 122.57, 112.17, 100.25, 91.64, 56.02, 46.27, respectively. The feeding value, grading index and the contents of dry matter, ether extract, crude ash and crude protein of different roughages were significantly different (P<0.01). The moisture content and feeding value and grading index of peanut straw among straw of roughage were the highest, and the contents of crude ash and crude fat were lower; The moisture content and feeding value and grading index of soybean hull among hull of roughage were the highest, and the contents of crude ash and crude fat were lower. Therefore, soybean hull and peanut straw can be developed and utilized as roughage of high nutritional value. [ABSTRACT FROM AUTHOR]

BACKGROUND: Serum-specific biomarkers between normal healthy individuals and populations with developmental cervical canal stenosis (Qi deficiency and blood stasis syndrome) have not been fully defined. OBJECTIVE: To screen and identify the potential biomarkers of developmental cervical canal stenosis with Qi deficiency and blood stasis. METHODS: Serum samples were collected from nine patients with developmental cervical canal stenosis with Qi deficiency and blood stasis and eight healthy people. Differentially expressed proteins in serum were screened and identified using isotope relative labeling and absolute quantification combined with liquid chromatography tandem mass spectrometry. Western blot was used to verify some significant differentially expressed proteins. RESULTS AND CONCLUSION: A total of 61 differentially expressed proteins (P < 0.05) were identified using tandem mass spectrometry techniques. Compared with the healthy normal population group, 14 differentially expressed proteins such as complement component C1q receptor, apolipoprotein A4, and C-C motif chemokine ligand 18 were significantly upregulated, while 47 differentially expressed proteins such as myosin light chain 3, mitochondrial translation elongation factor, and nucleolar phosphoprotein 1 were significantly downregulated. The results of gene ontology enrichment analysis indicated that these differentially expressed proteins might participate in molecular functions such as regulation of chromosomal tissue, mitochondrial membrane tissue, and muscle system processes. Protein-protein interaction network analysis showed that 38 common differential proteins, including complement component C1q receptor, apolipoprotein A4, C-C motif chemokine ligand 18, myosin light chain 3, mitochondrial translation elongation factor, and nucleolar phosphoprotein 1, were located at functional network nodes between healthy normal individuals and those with developmental cervical canal stenosis (Qi deficiency and blood stasis syndrome), and were closely related to the local energy metabolism of the cervical spine, the production of cervical vertebral osteocytes, and the formation of osteoclasts. The main differentially expressed protein myosin light chain 3 was validated using western blot assay, and the validation results were consistent with the proteomic results. To conclude, the preliminary discovery of differentially expressed proteins in serum between healthy normal individuals and those with developmental cervical canal stenosis (Qi deficiency and blood stasis syndrome) through absolute quantitative technology combined with liquid chromatography tandem mass spectrometry technology suggests that myosin light chain 3 may be a specific serum marker for developmental cervical canal stenosis (Qi deficiency and blood stasis syndrome). [ABSTRACT FROM AUTHOR]

The experiment was to investigate the effect of Clostridium butyricum instead of antibiotics on growth performance and serum biochemical indexes of rabbits. Fifty healthy male rabbits at 90 d of age were randomly divided into five groups, with ten replicates in each group and one rabbit in each replicate. Rabbits in test group â…  (control group) were fed with a basal diet, rabbits in group â…¡ (antibiotic group) were fed basal diet + aureomycin 100 mg/kg, and rabbits in group â…¢, group â…£ and group â…¤ were fed with basal diet supplemented with 100, 250 and 400 mg/kg Clostridium butyricum, respectively. The pre-test lasted for 7 d and the formal experiment lasted for 30 d. The results showed that the final weight of rabbits in group â…¢, group â…£ and group â…¤ were extremely significantly higher than that in control group, and the result in group â…¤ was extremely significantly higher than that in other groups (P<0.01), the average daily gain of rabbits in group â…£ and group â…¤ were extremely higher than those in group â…  and group â…¡ (P<0.01); the average daily feed intake of rabbits in group â…¢, group â…£ and group â…¤ were extremely significantly higher than that in control group (P<0.01);the feed to gain ratio of rabbits in group â…£ and group â…¤ were significantly lower than that in control group (P<0.05). Serum GLU and LDL-C in group â…¢, group â…£ and group â…¤ were extremely lower than those in group â…  and group â…¡ (P<0.01). The content of TC, TP, ALB and GLB in serum of rabbits in group â…¤ were extremely higher than those in group â…  and group â…¡ (P<0.01). The experiment indicates that adding 400 mg/kg Clostridium butyricum in diet can significantly improve the growth performance, promote lipid deposition, and improve immune function of rabbits. The experiment was to investigate the effect of Clostridium butyricum instead of antibiotics on growth performance and serum biochemical indexes of rabbits. Fifty healthy male rabbits at 90 d of age were randomly divided into five groups, with ten replicates in each group and one rabbit in each replicate. Rabbits in test group Ⅰ (control group) were fed with a basal diet, rabbits in group Ⅱ (antibiotic group) were fed basal diet + aureomycin 100 mg/kg, and rabbits in group Ⅲ, group Ⅳ and group Ⅴ were fed with basal diet supplemented with 100, 250 and 400 mg/kg Clostridium butyricum, respectively. The pre-test lasted for 7 d and the formal experiment lasted for 30 d. The results showed that the final weight of rabbits in group Ⅲ, group Ⅳ and group Ⅴ were extremely significantly higher than that in control group, and the result in group Ⅴ was extremely significantly higher than that in other groups (P<0.01), the average daily gain of rabbits in group Ⅳ and group Ⅴ were extremely higher than those in group Ⅰ and group Ⅱ (P<0.01); the average daily feed intake of rabbits in group Ⅲ, group Ⅳ and group Ⅴ were extremely significantly higher than that in control group (P<0.01);the feed to gain ratio of rabbits in group Ⅳ and group Ⅴ were significantly lower than that in control group (P<0.05). Serum GLU and LDL-C in group Ⅲ, group Ⅳ and group Ⅴ were extremely lower than those in group Ⅰ and group Ⅱ (P<0.01). The content of TC, TP, ALB and GLB in serum of rabbits in group Ⅴ were extremely higher than those in group Ⅰ and group Ⅱ (P<0.01). The experiment indicates that adding 400 mg/kg Clostridium butyricum in diet can significantly improve the growth performance, promote lipid deposition, and improve immune function of rabbits. [ABSTRACT FROM AUTHOR]

Objective: To investigate the expression and clinical significance of co-signal molecule B7-H3 in non-small cell lung cancer (NSCLC) cell lines and tissues. Methods: B7-H3 expression level was evaluated by RT-PCR and western blot in five NSCLC cell lines. Specimens from 121 participants were stained immunohistochemically. Staining was quantified using Image-Proplus software, and integrated optical density was measured on each section. Results: The constitutive expression of B7-H3 in all five cell lines was found. The expression of B7-H3 were higher than that in benign lesions and non-cancer tissues respectively (P<0.001). B7-H3 expression in well differentiated NSCLC was Inhigher than that in not well differentiated one (P<0.05).There was a significant association between expression of B7-H3 and lymph node metastasis. Conclusion: B7-H3 is overexpressed in NSCLC tissues and may be an important target for diagnosis or the therapy of the disease. [ABSTRACT FROM AUTHOR]

Feed grade fish oil is one of the commonly used animal energy feed materials in livestock and aquaculture. Fish oil is rich in n-3 series of polyunsaturated fatty acids, which can effectively increase the content of CLA in ruminant milk and meat. The rational development and utilization of fish oil resources in ruminant production can effectively improve the nutritional value. The paper briefly introduces the composition of amino acids in fish oil, reviews the effect of fish oil supplementation on meat quality of ruminants, to provide reference for rational exploitation and utilization of fish oil in ruminants. [ABSTRACT FROM AUTHOR]

Objective　To compare the predictive value of NoSAS score and STOP-Bang questionnaire score， and evaluate the feasibility of NoSAS score screening for OSAHS in physical examination population. Methods　Retrospective analysis of the polysomnography （PSG） monitoring results of 135 patients with suspected OSAHS in our health management center was made from January 2018 to August 2019，including the NOSAS score and the Stop Bang Questionnaire and patient information. Grouped by apnea-hypopnea index （AHI），the sensitivity， specificity， positive predictive value and negative predictive value of NOSAS score and Stop-Bang questionnaire score were calculated， and ROC curves were drawn so as to compare the effectiveness of two scores which screened OSAHS. Result　Of the 135 patients，75.6% were male and 24.4% were female. Using AHI ≥ 5 events/h， AHI ≥ 15 events/h and AHI ≥ 30 events/h as the diagnostic criteria， the sensitivity and specificity of NOSAS scores were： 0.891 and 0.720， 0.910 and 0.587， 0.814， and 0.553； The sensitivity and specificity of STOP-Bang questionnaire were 0.873 and 0.560，0.910 and 0.435，0.949 and 0.329，respectively. When AHI ≥ 5 events/h， AHI ≥ 15 events/h and AHI ≥ 30 events / h were used as diagnostic criteria， the areas under ROC curve of the No SAS score were 0.847，0.773 and 0.693，the difference was statistically significant （ P < 0.001）；the areas under the ROC curve of the STOP-Bang questionnaire were 0.784， 0.711， 0.694 and the differences were statistically significant （P < 0.001）. When AHI is 5 events/h，15 events/h and 30 events/h as the critical points， the area under the NOSAS score ROC curve is greater than or equal to the STOP-Bang questionnaire score. The NOSAS score has good predictive value. Conclusion　As a simple and effective initial screening tool， the NOSAS score can effectively help physicians quickly screen OSAHS patients in physical examination population. [ABSTRACT FROM AUTHOR]

Objective: To investigate the influence of iloprost inhalation on chemosensitivity of non-small cell lung cancer (NSCLC) cells. Method: A total of 40 patients with NSCLC, who were admitted to Affiliated Hospital of Chengdu University of Traditional Chinese Medicine from August 2014 to August 2015, were chosen as the research subjects and randomly divided into treatment group and control group. All the patients were treated with chemotherapy of gemcitabine combined with cisplatin, on the basis of which, the treatment group was added iloprost inhalation. The levels of serum HIF-1琢and VEGF in the two groups were detected by ELISA method before and after treatment, the physical condition of the patients was evaluated by Karnofsky score. The clinical effect and the incidence of side effects in the two group were compared. Results: The response rate (RR) and disease control rate (DCR) in the treatment group were significantly higher than those in the control group(P<0.05). Incidence of side effects in the treatment group was significantly lower than that in the control group (P<0.05). Serum HIF-1琢and VEGF levels in the two groups after treatment were lower than before treatment. The levels of serum HIF-1琢and VEGF in the treatment group 1 day and 21 days after treatment were lower than those in the control group in the same period, furthermore, the levels of serumHIF-1琢and VEGF in the two groups 21 days after treatment were lower than those 1 day after treatment（P<0.05）. Conclusion: Iloprost inhalation can improve the chemosensitivity of NSCLC cells by regulating the serumHIF-1 and VEGF levels, which has a good and safe effect, and satisfied prognosis. [ABSTRACT FROM AUTHOR]

Objective: To investigate the expression of vimentin in non-small cell lung cancer (NSCLC) tissues and the relationship with invasion and metastasis of lung cancer. Methods: Collected 150 cases of NSCLC carcinoma tissues and 79 cases of adjacent normal tissues (>5cm away from carcinoma tissues) from the resection underwent in our hospital from June 2012 to June 2014, then extracted RNA of two groups, detected the expression of vimentin mRNA by real time-polymerase chain reaction (RT-PCR) method, and detected the expression of vimentin protein by immunohistochemical method, finally, analyzed the relationship between the expression of vimentin protein and the lymph node metastasis and TNMstage. Results: The expression of vimentin mRNA expression in NSCLC carcinoma tissues was significantly higher than adjacent normal tissues(P<0.05). The up-regulation expression of vimentin mRNA was related with lymph node metastasis and TNMstage (P<0.05). Conclusions: The expression of vimentin is up-regulation in NSCLC, which is closely related to the occurrence and invasion of NSCLC. [ABSTRACT FROM AUTHOR]

Objective: To explore the diagnostic value of glucose regulated protein 78 (GRP78) of endoplasmic reticulum stress molecules for non small cell lung cancer (NSCLC). Method: A total of postoperative carcinoma tissue specimens of 45 patients with NSCLC were selected, from which 18 cases of adjacent to carcinoma tissues were selected at the same time. The expressions was used to of GRP78 of all the selected tissues were detected by Real-time PCR and analyzed the correlation of the expression of GRP78 in NSCLC tissues with clinical characteristics and the survival periods of the patients. Results: The expression levels of GRP78 in NSCLC tissues were much higher than those in para-cancerous tissues, the differences were statistically significant (p<0.05). The expression levels of GRP78 in the cancer tissues of patients with NSCLC at stage Ⅲ a were much higher than those at stage Ⅰ-Ⅱ, the differences were statistically significant (P<0.05). Kaplan-Meier analysis showed that the survival periods of patients with high expression level of GRP78 were shorter than low expression level of GRP78 (P<0.05). Conclusion: The expression of GRP78 May be related to the occurrence and development of tumor cells and the survival periods of patients, which can be used as an important biomarker of forecasting diagnosis and treatment of NSCLC patients. [ABSTRACT FROM AUTHOR]