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目的寻找与创伤性颅脑损伤（TBI）预后相关的急性期特征。方法回顾性分析354名TBI患者的人口学、急性期和慢性期特征，使用传统的基于关联分析和预测模型的策略以及一种基于特征解构的创新的研究策略，识别与预后指标-慢性期日常生活能力（ADL）受损相关的急性期特征。特征解构策略通过使用LASSO构建基于其他非ADL的慢性期指标预测ADL的模型，找到解释TBI人群ADL的关键慢性期特征维度，再分析与这些特征维度显著相关的人口学、急性期变量。结果特征解构策略将ADL在TBI人群中解构为“受伤后脑萎缩”“自知力受损程度”“四肢乏力”等慢性期特征维度，同时首次揭示了急性期特征与具体慢性期损伤特征的联系，如TBI患者昏迷时间长和GCS评分低时，慢性期“近记忆受损”的风险最大［scaled coma time OR95%CI = 94.288 （35.095， 273.231）； scaled GCS OR95%CI = 0.068 （0.030， 0.147）］；TBI患者有脑积水时，慢性期“自知力受损”和“定向力障碍”的风险最大［insight impairment OR95%CI = 6.760 （3.653，12.855）； disorientation OR95%CI = 6.538 （3.530， 12.490）］。所有策略均表明ADL受损最大的急性期风险因素为昏迷时间长、GCS评分低和有脑积水。结论本研究提出了一种新的建立TBI急性期特征和预后间关联的研究策略，识别了与预后指标ADL相关的人口学和急性期特征。

目的探讨老年人婚姻状况与轻度认知障碍（MCI）及痴呆患病率的相关性，为老年认知障碍防控提供社会学数据。方法采用整群随机抽样方法选取湖北省城乡65岁及以上老年人，在社区卫生服务中心采用面对面访谈形式完成一般情况调查和神经心理学评估，以及临床体检和实验室检查；由两名神经病学家和两名具有痴呆专业知识的神经心理学家组成诊断专家组，根据Petersen的MCI诊断标准和《中国痴呆与认知障碍诊断指南》对参与者进行MCI和痴呆诊断；采用多因素Logistic回归分析不同年龄段老年人婚姻状况与MCI及痴呆患病的相关性。结果在2018~2020年，共8 221人完成全套神经认知评估和临床体检，平均年龄71.96±5.90岁，有1 953人无配偶，占总人数的23.75%。MCI患病率为25.98%， 95%CI （24.99%，26.98%），痴呆患病率为7.24%， 95%CI （6.68%，7.80%），使用湖北省2010年人口普查数据进行年龄和性别标准化后，MCI和痴呆的患病率分别为26.28%和6.98%，无配偶者MCI及痴呆患病率［28.07%，95%CI （26.08%，30.06%）；12.45%，95%CI （10.99%，13.91%）］明显高于有配偶者［22.85%，95%CI （21.81%，23.89%）；5.62%，95%CI （5.05%，6.19%）］。多因素Logistic回归模型发现有配偶的65~69岁男性老人［P=0.028，OR=0.639，95%CI（0.429，0.952）］和80岁及以上男性老人［P=0.004，OR=0.460，95%CI（0.269，0.785）］的MCI和痴呆患病率显著低于无配偶者，然而女性老年人有无配偶与MCI和痴呆患病的关联性无差异统计性（P>0.05）。结论MCI和痴呆患病率在无配偶老年群体中增高，特别是针对65~69岁和80岁及以上的男性老人，有配偶对保护认知功能至关重要。

Copyright of Journal of Anhui Agricultural Sciences is the property of Journal of Anhui Agricultural Sciences Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Journal of Isotopes is the property of Journal of Isotopes Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In order to deeply investigate the antioxidant potential of essential oil from PK-type Perilla frutescens leaves, an essential oil with a content of perilla ketone higher than 40% was screened out by using the gas chromatographic analysis (GC) technology. Then, the chemical composition of the essential oil was further identified by using the GC-MS technology, and the antioxidant activity in vitro of the essential oil was analyzed, which provided a scientific basis for the development and utilization of the essential oil from PK-type Perilla frutescens leaves in the field of antioxidation. The results showed that there were 17 kinds of chemical components with the content of more than 0.5% in the essential oil from Perilla frutescens, all of which were terpenes and their derivatives, accounting for 89.72% of the total content of essential oil. The components with higher content were perilla ketone (44.98%), isoegomaketone (13.06%) and β-caryophyllene (10.53%). The experimental results of antioxidant activity in vitro showed that: the essential oil from PK-type Perilla frutescens leaves had scavenging ability to both DPPH and ABTS radicals, with IC50 values of 7.00 and 2.66 mg·mL-1, respectively. It also had certain iron reduction ability and total antioxidant capacity, with the RP0.5 AU values of 11.96 and 1.83 mg·mL-1. It was shown that all the above antioxidant activity indexes of the essential oil from PK-type Perilla frutescens leaves were positively correlated with the concentration. [ABSTRACT FROM AUTHOR]

The experiment aimed to screen for high-yielding hybrid combinations of male-sterile alfalfa lines. A total of 10 hybrid combinations were prepared with one alfalfa male sterile line (MS-GN) as the female parent and 10 excellent alfalfa plants (Alfalfa 354-05, Cavalier-02, Gannong NO. 8-04, Alfalfa 440-02, Queen-25, Alfalfa 319-01, Longdong Alfalfa-06, Alfalfa 343-02, Algonkin-19, and Suntory-74) as the male parents, and the numbers were M×1, M×2, M×3, M×4, M×5, M×6, M×7, M×8, M×9, and M×10. The high-yield hybrid combinations were screened by measuring the plant height, stem diameter, primary branch number, fresh yields and hay yields of the F1 generation of the hybrid combination for two consecutive years, combined with the analysis of general compounding ability. The results showed that M×4 had the highest two-year average plant height, which was significantly higher than that of M×7, M×3, M×5, M×8 and M×10 (P<0.05). The two-year average stem diameter of M×6 was the highest, and the two-year average primary branch number of M×1 was the highest. M×1 and M×6 had the highest two-year average hay yield of 2.02 kg/plant, while M×4 and M×1 had the highest two-year average hay yield of 0.60 kg/plant. Plant height, stem diameter, and primary branch number were positively correlated with grass yield, and plant height and primary branch number were significantly or extremely correlated with fresh yield and hay yield (P<0.05 or P<0.01). The cluster analysis of yield traits divided the hybrid combinations into four categories, among which the combinations with better yield traits were M×1, M×6, and M×4, which were consistent with the results of the comprehensive analysis of grey correlation degree. In the general compounding ability (GCA) effect value, M×4 had the highest plant height, M×6 had the highest stem diameter, M×1 had the highest number of first-order branches, M×1 and M×6 had the highest yield, and M×1 and M×4 had the highest hay yield. The results showed that M×1, M×4, and M×6 had better comprehensive performance in yield traits among the hybrid combinations, and they were high-yield hybrid combinations. [ABSTRACT FROM AUTHOR]

Stress is a common risk factor for emotional and cognitive dysfunction during mammalian growth and development. The hippocampal neurogenesis, which regulates the stress response, is highly susceptible to stress stimuli. This review focuses on the pathomechanism underlying how stress stimuli affect hippocampal neurogenesis and explores the impact of aberrant 5-hydroxytryptamine (5-HT) system, including 5-HT, 5-HT receptors and 5-HT neurons, on stressinduced impairment of hippocampal neurogenesis. By summarizing existing research, we aim to provide new ideas for the treatment of post-traumatic stress disorder and depression. [ABSTRACT FROM AUTHOR]

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Copyright of Journal of Agro-Environment Science is the property of Journal of Agro-Environment Science Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Journal of Shenyang Pharmaceutical University is the property of Shenyang Pharmaceutical University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Shenyang Pharmaceutical University is the property of Shenyang Pharmaceutical University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Anhui Agricultural Sciences is the property of Journal of Anhui Agricultural Sciences Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Perilla frutescens was rich in resources and low in cost in China, of which the leaves have been widely used in the field of medicine and spices. The essential oil was one of its main functional components, which was rich in a variety of active ingredients, with various effects such as antioxidation, antibacterial, and anti-corrosion, and also had great potential for development. Since the efficacy of essential oil extracted from the leaves of Perilla frutescens was closely related to its chemical composition, the extraction process, analysis method, chemical composition and chemical type of the essential oil extracted from the leaves of Perilla frutescens were summarized by systematically analyzing the relevant literature, in order to provide valuable reference for the further development and application of di任erent types of essential oil extracted from the leaves of Perilla frutescens in the future. [ABSTRACT FROM AUTHOR]

The experiment was to investigate the effect of N-carbamylglutamate on reproductive performance, serum indexes and hormone secretion of cows. Sixty Angus cows with similar weight, parity and expected delivery were randomly divided into four groups with 15 cows in each group, one cow per replicate. The control group was basal diet, and each cow in test group 1, group 2 and group 3 were fed with basal diet supplemented with 10, 20 and 30 g/(head·d) of NCG, respectively. The experiment lasted for 120 d. The results showed that after cows delivery, the content of GLU and NO of cows in test group 3 were significantly higher than control group (P<0.05), the content of Arg of cows in test group 2 and group 3 were significantly higher than control group (P<0.05), and the content of P of cows in test group 2 were significantly higher than control group (P<0.05),while the content of E2 of cows in test group 3 were significantly lower than control group (P<0.05). Before cows breeding, the content of serum BUN of cows in test group 2 and group 3 were significantly lower than control group (P<0.05), while the content of GLU in test group 2 was significantly higher than control group (P<0.05), the content of Arg and NO in all the test groups were significantly higher than control group (P<0.05), the content of serum E2 in test group 3 was significantly higher than control group (P<0.05). The content of serum P in test group 2 and group 3 were significantly lower than control group (P<0.05). The estrus mating rates of cows in test group 2 and group 3 were higher than control group, and the conception rates of experimental groups were higher than that in control group, but the first postpartum mating days in test group 2 and group 3 were significantly lower than control group (P<0.05). The results indicates that the NCG can improve body metabolism, promote the secretion of reproduction-related hormones, and improve the reproductive performance of breeding cows, and the appropriate supplemental level is 20 and 30 g/(head·d). [ABSTRACT FROM AUTHOR]

Objective: To explore the association between sensory decline and mild cognitive impairment (MCI) in urban and rural community-dwelling older adults. Methods: The data were collected from the 2018 -2021 baseline data of the Hubei Memory and Ageing Cohort Study. The cluster sampling method was used to select 7046 urban and rural residents aged 65 years and above in Dawu County and Wuhan City, Hubei Province. The sensory decline assessment, complete set of standard neuropsychological assessment and clinical physical examination were carried out. The diagnostic expert group referred to Petersen's diagnostic criteria to diagnose MCI. Chi-square test was used to analyze the differences between groups, and multivariate logistic regression model was used to analyze the correlation between sensory decline and MCI. Results: The detection rate of MCI was 21.4% (95% CI: 20.4 % -22.4%), higher in rural areas than in urban areas (39.8% vs. 10.0 %, P <0.05). Compared with those with- out sensory decline, the rural older adults with single sensory decline (OR = 1.26, 95 % CI = 1.03 -1.55) and double sensory decline (OR = 1. 32, 95 % CI: 1.08 -1.63) had a higher risk of MCI, and the urban older adults with double sensory decline (OR = 1.45, 95 % CI: 1. 10 - 1.92) increased the risk of MCI. Conclusion: Sensory decline is associated with MCI in the community-dwelling older adults aged 65 years and over, and the older adults with double sensory decline may be the focus of MCI prevention. [ABSTRACT FROM AUTHOR]

The experiment was conducted to investigate the effect of dietary NCG supplementation on growth performance, serum biochemical indexes and immune indexes of calves in Angus cows during late gestation. Sixty cows in late gestation period with similar weight parity and expected perinatal period were randomly divided into four groups with 15 cows in each group. Cows in control group were fed with a basal diet, cows in experimental groups were fed wtih basal diet supplemented with 10, 20 and 30 g/(head·d) NCG, respectively. The corresponding calves in each group were denoted as group 1, group 2 and group 3, respectively. The experiment starts from 4 w before delivery of cows and ends at 90 d of age of calves. The results showed that compared with control group, the birth weight of calves in group 3 was significantly higher than that in control group (P<0.05). Compared with control group, the contents of TP, ALB and NO in serum of newborn calves in group 3 were significantly increased (P<0.05), and the content of IgA, IgG and IgM in serum were significantly increased (P<0.05). The experiment indicates that the addition of NCG of 30 g/(head·d) to diet of cows in late gestation period can significantly increase birth weight, increase erum protein content of newborn calves, improve immune function, but these effects were lost at 90 days of age. [ABSTRACT FROM AUTHOR]

Objective To analyze the effect of down-regulation of BECN1 on the proliferation,invasion and migration of human leukemia cell line THP-1,and to explore its potential molecular mechanism. Methods The lentivirus expressing shRNA targeting at BECN1 and the negative control were constructed by RNA interference. THP-1 cells were divided into control group(control),negative control group(NC) and BECN1 interference group (BECN1-shRNA). Stable cell strains were selected by puromycin after infection by lentivirus. The down-regulation effect of BECN1 was identified by RT-qPCR and Western blot. MTT and Transwell assays were used respectively to detect the proliferation,invasion and migration of THP-1 cells. The expression of MMP 2 and MMP 9 were detected by Western blot. Results THP-1 stable cell strain expressing GFP was obtained by puromycin selection. Compared with the control and negative control group, the BECN1 mRNA and protein expression in the BECN1-shRNA group were significantly decreased (P<0.05); The proliferation,invasion and migration of cells also decreased significantly(P<0.05);The expression of MMP 2 and MMP 9 decreased (P<0.05). Conclusions Down-regulation of BECN1 may inhibit the proliferation,invasion and migration of THP-1 cells,and its molecular mechanism is related to the down-regulation of MMP 2 and MMP 9. [ABSTRACT FROM AUTHOR]

Data is a very important resource in digital economy and is also an important factor of production that stimulates China's economic development. In China, public data is the largest and most widely covered data type. Therefore, public data has enormous potential commercial and social value, and further development and utilization are urgently needed. At present, public data openness in China develops rapidly, but there are still many problems. Based on the existing literature, this paper summarizes the problems of public data opening in China into three aspects: insufficient platform coverage, poor data availability and regional differences. Based on the dialectical development perspective and the current practical situation, this paper points out that the enthusiasm of local governments plays a key role in the development of public data openness, and the lack of enthusiasm of local governments can comprehensively explain the problems of public data openness in China. Therefore, the lack of enthusiasm from local governments is the main factor hindering public data openness in China. On this basis, this paper points out that establishing a paid-use system of public data can effectively stimulate the enthusiasm of local governments, and this paper responds to the debate on establishing a paid-use system of public data. On the one hand, the paid use system of public data can bring benefits to local governments, allowing them to participate in the distribution of data source benefits, thereby enhancing their enthusiasm. By charging fee, local governments can get benefits when firms or agents use public data even when firms or agents are in other provinces. On the other hand, when local governments get benefits from public data openness, and they would promote the healthy and sustainable development of public data openness. The higher quality and more data attract more firms and agents using them, and bring more benefit to local government. However, establishing a paid use system of public data faces four controversies. Firstly, does the government have the right to charge fees? Secondly, is public data openness a public product? Thirdly, will paid-use system reduce economic efficiency? Fourthly, will paid use system lead to excessive reliance on data transfer fees by local finance? This paper responds to all controversies in the above. Finally, this paper proposes four policy recommendations based on the goal of promoting the enthusiasm of local governments and promoting the sustainable development of public data openness. These four recommendations make up a whole system which can stimulates the enthusiasm of local governments, provides macro economic tools for data market, and establishes a data trading system which is a sample for private data trading. [ABSTRACT FROM AUTHOR]

重症肺炎在儿科中是一种常见的严重疾病，可能导致多器官功能障碍和死亡。基于此，本文简要介绍了 重症肺炎对患儿的危害，分析了重症肺炎的病因与病理机制。并对重症肺炎的治疗方法、治疗效果评估与预后分析进 行了探讨。 [ABSTRACT FROM AUTHOR]

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Objective: To study the correlation between serum homocysteine (Hcy), visfatin (visfatin) and epithelial fatty acid binding protein (E-FABP) levels and glucose and lipid metabolism disorders and inflammatory factors in children with simple obesity. Methods: 70 children with simple obesity who were treated in our hospital from April 2019 to October 2020 were included in the study, and recorded as obesity group. 70 healthy children who underwent physical examination in hospital during the same period were selected as the control group. Serum Hcy, visfatin, E-FABP levels, glucose and lipid metabolism disorders and inflammatory factors were detected and compared between the two groups. Pearson correlation analysis was used to determine the relationship between serum Hcy, visfatin, E-FABP levels and glucose and lipid metabolism disorders and inflammatory factors in children with simple obesity. Results: The serum Hcy, visfatin and E-FABP levels in the obesity group were higher than those in the control group (all P<0.05). The Fasting blood glucose (FPG), fasting insulin (FINS) levels and insulin Resistance Index (HOMA-IR) in the obesity group were higher than those in the control group (all P<0.05). The total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) levels in the obesity group were higher than those in the control group, while the high density lipoprotein cholesterol (HDL-C) level was lower than that in the control group (all P<0.05). The serum interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) levels in the obesity group were higher than those in the control group (all P<0.05). According to Pearson correlation analysis: the serum Hcy, visfatin and E-FABP levels were positively correlated with the FPG, FINS, HOMA-IR, TC, TG, LDL-C, IL-1β, IL-6 and TNF-α levels, but negatively correlated with the HDL-C level in children with simple obesity (all P<0.05). Conclusion: Serum levels of Hcy, Visfatin and E-FABP are all abnormally elevated in children with simple obesity, which are closely related to glucose and lipid metabolism disorder and inflammatory response, and deserved clinical attention. [ABSTRACT FROM AUTHOR]

The study was to identify the suitable feeding oat varieties for planting in the hilly region of western Loess Platea. The experiment carried out the analysis of the yield, quality and other indicators for 13 imported forage oats and Guyuan big oats（CK group） varieties. The results showed that the three-year average of fresh grass yields of Danyan 444,Monte and Qinghai 444 were 21.79%, 25.32% and 28.08% lower than that of CK group, respectively（P<0.05）. The threeyear average hay yield of Danyan 444 and Qinghai 444 were 20.11% and 26.02% lower than CK group, respectively（P<0.05）. The three-year average DM content of Monida, Baylor, Monte, Haiwei, Tianyan 2, Danyan 444, Qinghai 444,Tianyan 1 and Muwang were significantly higher than CK group. CK group had the lowest three-year average of CP content. Monte’s three-year average of NDF content was significantly lower than CK group（P<0.05）, and RFV average was significantly higher than CK group（P<0.05）. The average values of Tianyan 1, Baylor, Tianyan 2 and Haiwei WSC were significantly higher than CK group（P<0.05）. According to quality grading standard of oat hay, 14 varieties were divided into two categories, and there were 13 varieties belonging to type A oat grass. Sweet Yan 2 was the second level,Dan Yan 444, Mu Lesi, Tian Yan 1, Baylor, Muwang, Ota, Qinghai 444, Guyuan Oats, Haiwei, Monte, Monida, Xiyue were the third level. There was one variety belonging to Type B oatgrass, Tianyan No. 1, which was the third grade. The study indicates that after comprehensive evaluation, Tianyan No. 1, Tianyan No. 2 and Baylor varieties are suitable for planting in the hilly region of the western Loess Plateau. [ABSTRACT FROM AUTHOR]

In the study, related indexes of production performance and nutritional quality of 15 alfalfa varieties were measured, and comprehensive evaluation of traits were evaluated by grey relational degree analysis, and the feeding value of varieties were compared. The results showed that the fresh grass yield of Gannong NO.6, Gannong NO.8 and WL298HQ was higher than Zhongmu NO.1. The hay yield of Gannong NO.6 was higher than Zhongmu NO.1, and the plant height of Gannong NO.6 was also better. The average yield of fresh yield and hay yield of Gannong NO.6 were increased 18.43% and 13.28% compared with Zhongmu NO. 1. There was no significant difference in DM content among different varieties (P>0.05). Longmu 801 had the highest CP content (20.76%), followed by Yong Shi, 4030, WL298HQ, 7G3, WL-SQT, 42IQ, Gannong NO.8 and Yan Bao, which were significantly higher than that of Zhongmu NO.1 (P<0.05). The results showed that Gannong NO.6, Longmu 801, 7G3, WL298HQ, Gannong NO.8, 42IQ, Yan Bao and Yong Shi were better by grey relational degree analysis. Therefore, the above eight varieties have high feeding value and were suitable for promotion and utilization in mountainous area of Southern Ningxia. [ABSTRACT FROM AUTHOR]

OBJECTIVE: Schwann cells can promote the regeneration of damaged peripheral nerves and serve as seed cells in the engineering repair of peripheral nerve tissue. The effective culture and purification of Schwann cells are the basis of clinical treatment for peripheral nerve injury. This paper summarized the literature of culture of Schwann cells in vitro in recent ten years and made a descriptive review on the research progress of Schwann cell culture and purification, in order to provide references for culture of Schwann cells in vitro. METHODS: Literature related to Schwann cell isolation, culture and purification was retrieved from PubMed, Web of Science, Medline databases, CNKI, Wanfang, VIP and other databases. The keywords included “Schwann cells; isolation; culture; purification”. All the papers obtained from the search were read, analyzed and judged, and finally 62 papers meeting the standards were included. RESULTS: The classical methods of Schwann cell culture include tissue block culture and enzyme digestion. Purification methods include mitosis resistance, immune selection, specific adhesion, pre degeneration, cold jet, differential adherent, laminin package, low serum, stimulating factor, fluorescence activated cell sorting or magnetic activated cell sorting, immunopanning, and extracorporeal shock wave treatment. CONCLUSION: Schwann cells can be cultured and purified in various ways in vitro, and each method has its advantages and disadvantages. How to obtain highpurity Schwann cells quickly and efficiently is still a challenge. Multiple methods can be combined for purification and affective factors can be controlled for Schwann cell proliferation as much as possible. [ABSTRACT FROM AUTHOR]

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