下调BECN1抑制人白血病细胞系THP-1增殖、侵袭和迁移.

Objective To analyze the effect of down-regulation of BECN1 on the proliferation,invasion and migration of human leukemia cell line THP-1,and to explore its potential molecular mechanism. Methods The lentivirus expressing shRNA targeting at BECN1 and the negative control were constructed by RNA interference. THP-1 cells were divided into control group(control),negative control group(NC) and BECN1 interference group (BECN1-shRNA). Stable cell strains were selected by puromycin after infection by lentivirus. The down-regulation effect of BECN1 was identified by RT-qPCR and Western blot. MTT and Transwell assays were used respectively to detect the proliferation,invasion and migration of THP-1 cells. The expression of MMP 2 and MMP 9 were detected by Western blot. Results THP-1 stable cell strain expressing GFP was obtained by puromycin selection. Compared with the control and negative control group, the BECN1 mRNA and protein expression in the BECN1-shRNA group were significantly decreased (P<0.05); The proliferation,invasion and migration of cells also decreased significantly(P<0.05);The expression of MMP 2 and MMP 9 decreased (P<0.05). Conclusions Down-regulation of BECN1 may inhibit the proliferation,invasion and migration of THP-1 cells,and its molecular mechanism is related to the down-regulation of MMP 2 and MMP 9. [ABSTRACT FROM AUTHOR]