Fungal community composition and diversity are important indicators for soil health. In order to explore the effects of biochar on the soil fungal community of Panax notoginseng under continuous cropping, biochar was applied at 0, 12, and 15 t/ha(T0, T1, and T2)to the soil which has cultivated P. notoginseng for 10 years. After transplanting for 18 months, soil fungal diversity and the abundance of pathogenic Fusarium oxysporum in the rhizosphere of P. notoginseng under different treatments were analyzed using high-throughput sequencing and qPCR technology. Moreover, the correlations between soil physicochemical factors and changes in fungal community, and the effects of biochar on the survival rate of P. notoginseng were explored. The results showed that soil pH, NO3--N, and available potassium(AK)contents in the rhizosphere soil increased by 6.5%, 13.6%, and 40.3% respectively under T2 treatment, however, soil NH4+-N content decreased by 21.2%. Biochar application significantly increased fungal α diversity and altered fungal community composition. At the phylum level, the relative abundance of Mortierellomycota significantly increased under biochar treatment. At the genus level, while the relative abundance of Fusarium decreased, the relative abundance of Mortierella increased significantly(P<0.05), qPCR analysis showed that the abundance of pathogenic F. oxysporum significantly decreased with biochar application(P<0.05). The survival rate of P. notoginseng increased by 24.0% under T2 treatment, which was positively correlated with soil pH, fungal α diversity, and relative abundance of Mortierella, but negatively correlated with NH4+-N content and F. oxysporum abundance(P<0.05). Therefore, the application of biochar improved soil physicochemical properties, changed soil fungal community, increased the abundance of beneficial fungi, and decreased the abundance of pathogenic F. oxysporum, which effectively increased the survival rate of P. notoginseng under continuous cropping. Our data indicated that biochar was effective to reduce the replant problem of P. notoginseng. [ABSTRACT FROM AUTHOR]