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羽毛是鸟类研究、监测和管理中常用的非损伤性材料，可从中提取DNA和激素进行遗传和生理分析。然而，作为高度角化的组织，羽翈中的DNA和激素含量极低，提取较为困难。本研究开发一种能够从羽翈中同时提取总DNA和糖皮质激素的方法，命名为DH-CoEx。以丹顶鹤（Grus japonensis）、绿孔雀（Pavo muticus）为代表的大型鸟类廓羽、尾上覆羽和以栗鹀（Emberiza rutila）、小鹀（E.pusilla）为代表的小型鸟类飞羽、绒羽进行测试和评估。结果显示：DH-CoEx能有效从完全角化的羽翈中同时提取出总DNA及糖皮质激素（皮质醇）；从5 mg样本中提取的线粒体DNA（mtDNA）拷贝数达到104量级，可支持长达1 350 bp的片段扩增；所提取的核DNA（nuDNA）质量足以进行长达200 bp的微卫星等位基因分型，成功率为98.11% ~ 99.60%；提取的皮质醇含量达到了传统单一提取激素方法的水平，足以进行相关生理学分析。以上结果说明，DH-CoEx是一种简易、高效和适用性广泛的方法，实现了角化材料总DNA和糖皮质激素的共提取，可以支持mtDNA和微卫星等分子标记的分析和应激激素的分析，极大地提高了羽毛的利用率，具有广泛的应用潜力。

Most existing recommendation models focus on explicitly constructing the relationship between users and items, neglecting to model the high-order global features of graph structure and under-mining the implicit interests of users. There- fore, this paper proposed a social knowledge recommendation based on graph reconstruction (SKRGR), which introduced graph reconstruction technology to decompose the user-item knowledge graph into multiple subgraphs, and utilized relationship- based graph attention networks and three-layer graph neural networks to encode them independently. By implementing the neighborhood enhancement strategy, it deeply explored users' implicit interests and facilitated the modeling of neighborhood local features. To further improve the quality of the node representations, this paper introduced a global contrastive learning mechanism to unify the node representations of the global interaction graph and the local collaboration graph, and used a gate fusion strategy to control the aggregation of global and local information. Experimental results on the Ciao and Epinions social datasets indicate that the SKRGR model achieves an average improvement of 8.77% in recall and 13.40% in NDCG. Com- pared to baseline models such as DSL and CLDS, SKRGR demonstrates significant performance advantages, validating its effectiveness in capturing users implicit interests and modeling global features. [ABSTRACT FROM AUTHOR]

Copyright of Mountain Research (10082786) is the property of Mountain Research Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Mountain Research (10082786) is the property of Mountain Research Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

绿孔雀(Pavo muticus)是备受关注的濒危物种。我国南方曾广泛分布的云南亚种(P.m.imperator)目前收缩到中部、南部和西部的局部地区,近年来陆续开展了野外种群调查,但遗传多样性的分析和评估尚未开展。本研究利用从玉溪市新平和峨山交界处采集的野外绿孔雀自然脱落的羽毛,采用11对微卫星标记分析遗传多样性和结构,并与国内主要圈养种群(建群者来自瑞丽地区和其他未确定的地点)进行比较。结果表明:该野生种群的遗传多样性较低,受到近交的影响,遗传多样性进一步丢失的风险较高。遗传结构上,该野生种群和圈养种群的个体被划分为3个亚群,野生种群作为独立的1个亚群,在遗传上有别于圈养种群所含的2个遗传谱系,说明云南省分布的绿孔雀有着显著而复杂的地理分化。当前需要开展系统的谱系地理学研究,以加强族群之间的基因交流为目标,通过生态廊道或个体转移等方式增加遗传交流的机会,扭转遗传多样性随近交的深化而不断丢失的趋势。

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Copyright of Journal of Jilin University (Science Edition) / Jilin Daxue Xuebao (Lixue Ban) is the property of Zhongguo Xue shu qi Kan (Guang Pan Ban) Dian zi Za zhi She and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Contemporary Pediatrics is the property of Xiangya Medical Periodical Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Journal of Engineering Management / Gongcheng Guanli Xuebao is the property of Journal of Engineering Management Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Wildlife / Yesheng Dongwu Xuebao is the property of Chinese Journal of Wildlife Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The aqueous zinc ion batteries (AZIB) have attracted attention due to their low cost, high safety, and environmental friendliness. Currently, the cathodes of AZIB are mainly inorganic materials, but their sluggish ionic diffusion kinetics, low Coulombic efficiency, poor cycling stability, and environmental harmfulness severely limit their practical applications. Covalent organic framework materials (COF) have gained attention as a new type of cathode materials due to their high specific surface area, high porosity, good electrochemical performance, and environmental friendliness. This review summarizes the progress of COF serving as cathode materials of AZIB, including the design strategies and performance of carbonyl-containing, nitrogen-containing and other cathodes, and also discusses the current challenges and future development opportunities. [ABSTRACT FROM AUTHOR]

Objective To investigate the influence pathway of the level of discrimination experienced by diabetic patients on psychological insulin resistance. Methods A multi-stage stratified random sampling method was used to select diabetic patients from 27 villages in Shandong Province for a questionnaire survey, with 660 valid responses received. The survey employed a general information questionnaire, the World Health Organization-5 Well-Being Index (WHO-5), and a scale assessing attitudes towards insulin. Results The score for psychological insulin resistance among diabetic patients was (72.97±11.38), and the WHO-5 score was (17.93±4.68). There were correlations among the level of discrimination experienced by patients, their physical and mental health, and psychological insulin resistance. The direct effect value of discrimination on psychological insulin resistance was 2.245 (bias-corrected 95%CI: 1.023-3.403), and the indirect effect value was 0.368 (bias-corrected 95%CI: 0.065-0.758). Conclusion Diabetic patients exhibit moderate to high levels of physical and mental health, with significant psychological insulin resistance. Physical and mental health plays a mediating role between the level of discrimination experienced by patients and psychological insulin resistance. [ABSTRACT FROM AUTHOR]

Objective To investigate the spatial and temporal evolution patterns of pension resource allocation across various provinces in China and to explore the spatial adaptability relationship and influencing factors between pension resource allocation and new urbanization development, providing recommendations for their balanced development. Methods An evaluation index system for pension resource allocation and new urbanization development levels in China was established. The coupling coordination model and Tobit model were employed to analyze the spatial adaptability and influencing factors of pension resources and new urbanization development. Results Between 2012 and 2021, there was a significant improvement in the levels of pension resource allocation and urbanization across 31 provinces in China; however, both exhibited notable regional disparities. The spatial adaptability between pension resource allocation and new urbanization levels required enhancement, with evident regional differences. Technological promotion and government guidance significantly impacted the spatial adaptability of pension resource allocation and new urbanization. Conclusion Macro-control should leverage government policy guidance and financial support. Technological development should facilitate the efficient utilization and effective scheduling of resources, and regional radiative effects should be harnessed to ensure unobstructed resource circulation channels and coordinated regional development. [ABSTRACT FROM AUTHOR]

Objective To evaluate the clinical effect of using a new cross buccal tube (XBT) to close the extraction gap of the maxillary second premolar and control the anchorage. Methods Fifteen adolescent patients with moderate to severe crowding, skeletal Class Ⅰ, missing maxillary second premolars or difficult to retain during clinical examination were selected. PASS orthodontic technique was used to reduce the number of maxillary second premolars to relieve crowding and retract the anterior teeth. Before and after treatment, head lateral images were taken and cephalometric measurements were taken. Changes of bone and dental indexes related to molar anchorage and anterior adduction were observed. Results After treatment, teeth of all patients were arranged neatly, the occlusion relationship was good and the profile was improved. Before and after treatment, there were no significant changes in bone indicators such as ∠SNA, ∠SNB and ∠ANB (P＞0.05). There was no significant change in vertical height ∠MP-SN (P＞0.05). The angle of the upper and lower central incisors, ∠U1-SN, ∠U1-NA, ∠ L1-MP and ∠L1-NB decreased significantly, while ∠U1-L1 increased significantly (P＜0.05). The upper incisor margin point moving towards the distal (P＜0.05), and there was no significant change in the position of the upper incisor root apex and first molar (P＞0.05). Conclusion The application of the new cross buccal tube is beneficial for adolescent patients to control the occlusion of the extraction gap of the maxillary second premolar. [ABSTRACT FROM AUTHOR]

Copyright of Modern Preventive Medicine is the property of Modern Preventive Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of China Plastics / Zhongguo Suliao is the property of Journal Office of CHINA PLASTICS and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Anhui Agricultural Sciences is the property of Journal of Anhui Agricultural Sciences Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Liaoning Manchu embroidery is an embroidery craft of ethnic minorities in Liaoning region, and it is also an art blending Manchu and Han cultures. It is unique with simple patterns and a rough and bold northern style. Its intangible cultural heritage has played an important role in Liaoning cultural inheritance. Liaoning Xiuyan and Jinzhou Manchu folk embroidery were inscribed onto the second batch of national intangible cultural heritage list in 2008. The intangible cultural heritage characteristics of Liaoning Manchu embroidery have played an important role in the cultural inheritance of Liaoning, promoting the development of Liaoning's textile and clothing industry. Through investigation, research, and interviews of Manchu embroidery in various parts of Liaoning province, this study analyzes the intangible cultural heritage characteristics of Manchu embroidery in Liaoning province, including its history and inheritance, regional and ethnic characteristics, culture and artistry, folk customs and practicality, and intangible cultural heritage inheritors and skills. Based on field research and graphic analysis, this study summarizes the intangible cultural heritage value of Manchu embroidery in Liaoning province, and takes its future inheritance methods and diversified development paths into full consideration. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Chongqing University of Technology (Natural Science) is the property of Chongqing University of Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of South Architecture / Nanfang Jianzhu is the property of South Architecture Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Functional Materials / Gongneng Cailiao is the property of Chongqing Functional Materials Periodical Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Plastics / Zhongguo Suliao is the property of Journal Office of CHINA PLASTICS and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of New Media Research is the property of New Media Research Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To analyze policy documents related to healthy city initiatives, assess the current state and shortcomings of policy tools, and provide a basis for adjusting and optimizing healthy city policies. Methods Using Rothwell & Ziegfeld’s policy tools framework, a two-dimensional analysis was conducted on 26 collected policy texts. These texts were coded, classified, and subjected to frequency statistics regarding the utilization of policy tools. Results In terms of policy tools, environmental tools accounted for 57.18%, supply tools for 38.11%, and demand tools for 4.72% of the policies analyzed. Across dimensions of health action, omnidirectional interventions were the most frequently utilized at 50.98%. Notably, actions promoting a healthy environment were prevalent, constituting 13.80% of the specific policy implementations. Conclusion The application of policy tools in China’s healthy cities exhibits uneven distribution, with an overemphasis on environmental tools, inadequate utilization of demand-side tools, and overall imbalance among policy tools. It is recommended that localities adjust their use of policy tools based on local conditions, leverage the distinct roles of various tools in healthy city initiatives, and ensure effective implementation of these policies. [ABSTRACT FROM AUTHOR]

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Ligularia virgaurea is one of the original plants of the Tibetan medicine “Rixiao” for the treatment of clearing heat and removing yellow water. In order to study the chemical constituents and anti-inflammatory activity of L. virgaurea, the compounds were separated by silica gel, Sephadex LH-20 gel, ODS gel column chromatography and other column chromatography technologies. The structures of all isolates were identified by spectroscopic methods(NMR and HR-ESI-MS). Their inhibitory activity of the compounds on nitric oxide(NO)was determined by lipopolysaccharide(LPS)-induced RAW264.7 cell model. The results were as follows:(1)Twenty-one compounds were separated and identified from petroleum ether and n-butanol extracts of L. virgaurea, including spiroeuryolide(1), cacalol acetate(2), oplopenone(3), 8-ethyl-palmosalide A(4), 1-hydroxy-3,7-dimethyl-2-(pent-3-enyl)benzofuran(5), syringaresinol-O-β-D-glucopyranoside(6), pinoresinol-O-β-D-glucopyranoside(7), isoeucommin A(8), eucommin A(9), 6,7-dimethoxycoumarin(10), ferulic acid(11), ethyl caffeate(12), methyl caffeate(13), methyl ferulate(14), ethyl ferulate(15), caffeic acid(16), 2-[(2'E)-3',7'-dimethyl-2',6'-octadienyl]-4-methoxy-6-methylphenol(17), 2,8-dimethyl-6-methoxy-2-(4'-methylpent-3'-enyl)-chromene(18), β-sitosterol(19), dodecyl(Z)-9-hexadecenoate(20)and hexacosanal(21). Compounds 1-4, 6, 11-16, 18, 20, 21 were isolated from the whole herbs of L. virgaurea for the first time.(2)The anti-inflammatory activity in vitro showed that compounds 1-3, 6, 11-16, 17, 19 could significantly inhibited releases of NO at concentration ranging from 1.56 to 50.00 μmol·L-1(P<0.05 or P<0.01), Compound 5 had no inhibitory effect on the release of NO at a concentration of 50.00 μmol·L-1, but it could inhibit releases of NO at concentrations of 12.50, 25.00 μmol·L-1(P<0.05). This finding enriches the chemical constituent and biological activity research of L. virgaurea and provides a certain theoretical reference for the future development and utilization of its anti-inflammatory activity. [ABSTRACT FROM AUTHOR]

This paper examines the challenges faced by graduate students pursuing professional degrees in materials and chemical engineering., It proposes a "project-based" training model, featuring collaborative training bases, reformed admission processes, “dual-platform” teaching, and integrated “professional practice-scientific innovation practice-dissertation” approaches. The “dual mentor system” and “enterprise-school evaluation” integration are key components. These methods aim to significantly enhance the training of high-level innovative versatile talents in the materials and chemical industry. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Clinical Nursing in Practice is the property of Journal of Clinical Nursing in Practice (Editorial Board, Shanghai Jiao Tong University Press) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Objective: To investigate the effects of methionine restriction on the proliferation, apoptosis, and ferroptosis of osteosarcoma cells. Methods: Three human osteosarcoma cell lines, MNNG-HOS, U2OS and Saos-2, were treated with normal or methionine-restricted medium, and the effects of methionine restriction on cell growth were assessed by cell counting; High-throughput sequencing (RNA-seq) was employed to analyze the molecular changes in osteosarcoma cells following methionine restriction; Flow cytometry was used to evaluate the cell cycle, cell apoptosis, reactive oxygen species (ROS), and lipid ROS levels; Western blot analysis was conducted to determine the protein expression of key ferroptosis biomarkers GPX4 and COX2. Results: Methionine restriction significantly inhibited the proliferation of osteosarcoma cells (P<0.001); RNA-seq analysis identified 1719 differentially expressed genes. The enrichment analysis indicated that the Ferroptosis pathway was significantly activate, and cell cycle pathway were significantly inhibited (P<0.001); Cytological experiments showed that methionine restriction induced G2/M Cell Cycle arrest and apoptosis in osteosarcoma cells; Besides, methionine restriction could induce ROS, lipid ROS level, and decrease GPX4 protein expression, upregulate COX2 protein expression in osteosarcoma cells. Moreover, methionine restriction Induced ferroptosis could be partially rescued by ferrostatin-1. Conclusion: Our findings provide potential novel therapeutic approaches or combination therapies for treating osteosarcoma while offering a theoretical basis for utilizing methionine restriction therapy against osteosarcoma. [ABSTRACT FROM AUTHOR]

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Copyright of Journal of Northwest A & F University - Natural Science Edition is the property of Editorial Department of Journal of Northwest A&F University (Natural Science Edition) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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