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(Objective) Amylose content is a key trait affecting the quality of glutinous rice. The present paper aimed to analyze the important significance of its genetic basis for rice quality improvement. (Method) In the study, we constructed the BC2F4 and BC2F5 backcross genetic generation populations of Pinnuo R191/Jinguisimiao//Jinguisimiao///Jinguisimiao' in a genetic background carrying the wxwx gene, and used the group segregation analysis method to SNP-type both parents and the high and low pooled populations using the GSR40K rice Gene- Chip, candidate genes affecting amylose content in rice were anchored according to the analysis results. (Result) Two candidate gene segments controlling amylose content were located on chromosome 5 from 0.04 to 0.37 Mb and chromosome 12 from 17.53 to 24.09 Mb. 14 pairs of polymorphic SSR markers and SNP markers were screened from the candidate intervals of chromosome 12, and the genetic linkage map was constructed by using QTL IciMapping software. By using Inclusive Composite Interval Mapping (ICIM), QTL qAC12 affecting amy- lose content was detected in the interval of N21774-A24633 with an interval distance of 3.46 Mb. The mean contribution rate to amylose content was 14. 10%, and the additive effect value was -0.11. For the analysis of candidate QTL at the end of chromosome 5, based on the genotyping results of the predicted interval marker Zc15_167, combined with the phenotype estimation, the contribution rate of QTL qAC5 to the phenotype of amylose content was 9.83%, and the additive effect value was 0. 156. 【Conlusion】 The amylose content of rice is not only regulated by Wx gene, but also closely related to other QTLs genes. It is of great significance for the breeding of new varieties of high quality rice to excavate and analyze its genetic basis. In the study, the micropotent QTL on chromosome 12 and chromosome 5 affecting amylose content of glutinous rice is identified, which can be helpful for molecular marker-assisted breeding of amylose content of glutinous rice, and has good practical value for reducing amylose content of glutinous rice and improving the quality of glutinous rice. [ABSTRACT FROM AUTHOR]

Strategic evaluation is the key link of military strategic system and capacity building. Selecting and evaluating the system construction scheme can optimize strategic decision-making and improve strategic management level to a certain extent. This paper constructs an intelligent selection analysis framework of "prospect cost" system construction scheme based on MATE, and uses NSGA-Ⅱ intelligent optimization algorithm to find the Pareto front of alternative scheme in multi-dimensional tradeoff space, which improves the problem of large data volume. Taking a joint operation system as an example, the effectiveness of the proposed method is verified, providing theoretical support for the optimization and evaluation of system construction scheme. [ABSTRACT FROM AUTHOR]

Objective: To analyze the expression and diagnostic value of plasma irisin and growth differentiation factor-8 in elderly patients with sarcopenia. Methods: A total of 620 elderly patients who received physical examination in our hospital from April 2020 to April 2022 were selected as the study subjects, including 238 patients with sarcopenia as the observation group and 382 patients without sarcopenia as the control group. The expression levels of plasma irisin and growth differentiation factor-8 in the two groups were detected to observe the differences of plasma irisin and growth differentiation factor-8 in elderly patients with different degrees of sarcopenia. Pearson correlation was used to analyze the relationship between plasma irisin, growth differentiation factor-8 and total skeletal muscle mass, limb skeletal muscle mass, relative skeletal muscle index,grip strength and walking speed, and the diagnostic efficacy of plasma irisin and growth differentiation factor-8 in senile sarcopenia was evaluated by area under receiver operating characteristic curve (AUC). Results: The expression level of plasma irisin in the observation group was lower than that in the control group, and the expression level of growth differentiation factor-8 was higher than that in the control group (P<0.05). The expression level of plasma irisin in patients with pre-sarcopenia, sarcopenia and severe sarcopenia was decreased successively, and the expression level of growth differentiation factor-8 was increased successively (P<0.05). Pearson correlation analysis showed that body skeletal muscle mass, limb skeletal muscle mass, relative skeletal muscle index, grip strength and walking speed were positively correlated with irisin in senile sarcopenia patients (P<0.05), and negatively correlated with growth differentiation factor-8 (P<0.05). ROC curve analysis showed that the sensitivity, specificity and AUC of plasma irisin and growth differentiation factor-8 for the diagnosis of senility sarcopenia were 78.49 %, 90.67 % and 0.914. Conclusion: The expression of plasma irisin and growth differentiation factor-8 in senile patients with sarcopenia is significantly down-regulated, which are closely related to the severity of the disease, the combined diagnosis of this disease is effective and worthy of clinical attention. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of TRB3 gene knockout on skeletal muscle atrophy and fibrosis in diabetic mice.Methods: Thirty TRB3 knockout(TRB3-/-) mice and 30 C57/BL6J mice were randomly divided into 4 groups, including TRB3 knockout diabetes model group(group A), normal TRB3 knockout control group(group B), normal control group(group C) and diabetes model group(group D). Normal control group mice were fed standard rat feed, and diabetic mice were injected intraperitoneally with low dose of streptozotocin to establish diabetic mice model. The forelimb grip strength test, suspension grid test and muscle function test were performed. Meanwhile, the levels of bone microstructure, anti-type I collagen and anti-type III collagen, atrophy gene MuRF1 and atrophy gene atrophy 1 were determined. Results: The hand grip strength of group A, B and D was significantly lower than that of group C, and the hand grip strength of group D was the lowest(P＜0.05). The inverted hanging time of mice in groups A, B and D was significantly lower than that in group C, and the inverted hanging time of mice in group D was the shortest(P＜0.05). CSA value of muscle function in groups A, B and D was significantly lower than that in group C, and CSA value of muscle function in group D was the lowest(P＜0.05). The bone microstructure indexes BMD, BV/TV, Tb.N and Tb.Th in groups A, B and D were significantly lower than those in group C, and the lowest values were in group D(P＜0.05). The values of Tb.Sp and SMI in group C were significantly higher than those in group C, and the values in group D were the highest(P＜0.05). The levels of anti-type I collagen and anti-type III collagen in groups A, B and D were significantly higher than those in group C, and the levels of anti-type I collagen and anti-type III collagen in group D were the highest(P＜0.05).The levels of atrophy gene MuRF1 and Atrogin-1 in groups A, B and D were significantly higher than those in group C, and the levels of Mu RF1 and Atrogin-1 in group D were the highest(P＜0.05). Conclusion: TRB3 gene knockout significantly reduced skeletal muscle atrophy and fibrosis and increased bone strength in diabetic mice. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effects of exosome miR-338 on bone metabolism, trabecular microstructure and bone biodynamics in osteoporosis rats. Methods: Bone marrow mesenchymal stem cells (BMSCs) were isolated from healthy adult SPF SD male rats. A rat model of osteoporosis was established by bilateral ovariectomy. The expression level of miR-338 was detected by qRT-PCR. Bone mineral density, trabecular microstructure and bone biomechanics were measured. Results: Compared with blank control group, the expression level of miR-338 was higher in OP model group, OP+ ExoBMSCs, inhibition group and overexpression group (P<0.05). The expression level of miR-338 in inhibition group was lower than that in OP model group, OP+ ExoBMSCs and overexpression group (P-0.05). Compared with blank control group, the expression levels of OC, PINP and BALP in OP model group, OP+ ExoBMSCs, inhibition group and overexpression group were lower (P<0.05). The expression levels of OC, PINP and BALP in inhibition group were higher than those in OP model group, OP+ ExoBMSCs and overexpression group (P<0.05). Compared with blank control group, the levels of BV/TV, Th.N, Tb.Th and Conn. D in OP model group, OP+ ExoBMSCs, inhibition group and overexpression group were lower, Tb.Sp and SMI were higher (P<0.05). The levels of BV/TV, Th.N, Tb.Th and Conn.D in inhibition group were higher than those in OP model group, OP+ ExoBMSCs and overexpression group (P<0.05), Tb.Sp and SMI were lower (P<0.05). Compared with blank control group, the levels of BMD, maximum load, maximum stress, maximum displacement and stiffness in OP model group, OP+ ExoBMSCs, inhibition group and overexpression group were lower (P<0.05). The levels of BMD, maximum load, maximum stress, maximum displacement and stiffness in inhibition group were higher than those in OP model group, OP+ ExoBMSCs and overexpression group (P<0.05). Conclusion: BMSCS-derived miR-338 can affect bone metabolism, trabecular microstructure and bone biomechanical status in osteoporosis rats. [ABSTRACT FROM AUTHOR]

目的：探讨焦虑障碍患者童年创伤经历与成人依恋之间的关系。方法：选取某精神专科医院 住院及门诊焦虑障碍患者１６６人（焦虑障碍组） 和专科医院及综合医院医务人员２９２人（对照组），运用 儿童期创伤问卷（ＣＴＱ）、亲密关系体验问卷（ＥＣＲ）进行测评。结果：焦虑障碍组的ＣＴＱ总分及各维度 得分、ＥＣＲ依恋回避及依恋焦虑维度得分均高于对照组（均Ｐ＜００１）。回归分析结果显示，所有被试（ｎ ＝４５８）ＣＴＱ总分正向预测依恋回避和依恋焦虑得分（β＝０６７、０６６，均Ｐ＜００１）；焦虑障碍组ＣＴＱ性 虐待得分正向预测依恋回避得分（β＝０１４，Ｐ＜００５），ＣＴＱ躯体忽视得分正向预测依恋焦虑得分（β＝ ０２１，Ｐ＜００５）。结论：童年创伤经历中的情感忽视与躯体忽视是依恋回避与焦虑出现的危险因素，创伤 经历对成人依恋的影响存在组别差异。 [ABSTRACT FROM AUTHOR]

Objective To investigate the relationship between the slimming of young women and the occurrence of senile osteoporosis, by studying the correlation between peak bone mass (PBM) of lumbar vertebrae and body fat distribution in young women. Methods A group of 25-35 year-old female volunteers were divided into two groups according to body mass index (BMI) ; lean type (BMI < 21. 4) and fat type (BMI ≥ 21. 4). General information including height, weight, waist circumference (WC) and hip circumference (HC) of each volunteer was collected to calculate BMI and waist-hip ratio (WHR). The abdominal visceral fat area (VFA) and subcutaneous fat area ( SFA) at the umbilicus level, and the BMD of lumbar vertebrae 2-4 were measured by Mindways quantitative CT (QCT) Pro analysis system. The ratio of VF/SF, and the average lumbar BMD (vBMD) were calculated. The differences in WHR, VFA, SFA, VF/SF and lumbar spine BMD between groups were compared using independent sample t test, and the correlation between vBMD and anthropometric parameters was analyzed using Pearson correlation test. Results A total of 95 volunteers were included, of which 43 were thin and 52 were fat. There were significant differences in VFA and SFA between the two groups, with P values of 0.01 and 0.003, respectively. There was no significant difference in WHR, VF/SF and vBMD between the two groups. There was significant correlation between vBMD and VF/SF in young women (r=0.550, P = 0.001). Conclusion The PBM of lumbar vertebrae was not correlated with BMI, WC, HC, VFA and SFA in young women, but significantly correlated with the ratio of VF / SF. Scientific slimming in young women would not reduce the BMD reserve. [ABSTRACT FROM AUTHOR]

Aiming at the problems that the image processing system in the field of machine vision has high real-time requirement, and the traditional FPGA design needs long development cycle, but high level synthesis (11LS) provides a new way to accelerate the machine vision algorithm. Based on ZC706 development platform and SDSoC development environment ofXilinx, this paper uses some optimization methods such as pipeline, loop unrolling, parallelism, line buffer and window buffer co-processing to realize hardware acceleration of the Sobel edge detection and Harris corner detection algorithm, which are widely used in the machine vision field. The experimental results show that the speed-up ratios of FPGA implementation based on 1ILS method for Sobel and Harris can reach 7. 37 and 19.41 , respectively, compared with software implementation in ARM platform. It can meet the requirements of real-time performance in the filed of machine vision. [ABSTRACT FROM AUTHOR]

Objective: To explore the clinical effect of hysteroscope in treatment of intrauterine adhesion(IUA) and analyze the risk factors for IUA. Methods: A total of 213 patients, who underwent abortion in University City Affiliated Hospital of Medical University Of Chongqing from June 2014 to June 2015, were selected and divided into adhesion group(n=103) and no adhesion group(n=110) according to the status of IUA, and the adhesion group underwent surgery under hysteroscope. The basic information and relevant disease history of the two groups were recorded. The risk factors of IUA and the curative effectof adhesion group were analyzed. Results: The single factor analysis showed that there were significant differences in the gravidity times, negative pressure of suck palace and time of suck palace between the two groups(P<0.05). Multiple factor analysis showed negative pressure of suck palace and time of suck palace were the risk factors for IUA(P<0.05). The total effective rates of mild, moderate and severe IUA patients were 100. 00%, 88. 24% and75. 00% respectively after operation under hysteroscopy, the differences were statistically significant(x2=13. 870, P=0.001). Conclusion:Negative pressure of suck palace and time of suck palace are the risk factors of IUA, and hysteroscope has a good effect in treatment of IUA, which is worthy of clinical application. [ABSTRACT FROM AUTHOR]

BACKGROUND: For non-traumatic osteonecrosis of the femoral head, if the femoral head collapses, it will have a great impact on the normal life of the patients. Thus, it is necessary to use an appropriate way to evaluate the risk of femoral head collapse and then to take targeted measures to delay the process of femoral head collapse. OBJECTIVE: To analyze the natural course of early osteonecrosis of the femoral head (without collapse) under different locations of necrotic lesions. METHODS: 121 patients (191 hips) with early non-traumatic osteonecrosis of the femoral head who were treated in the Outpatient Department of Honghui Hospital Affiliated to Xi’an Jiaotong University from October 2016 to October 2017 were enrolled in this study. The clinical data of all patients were followed up for 5 years to observe the collapse of osteonecrosis of the femoral head and the risk coefficient of femoral head collapse among different JIC types. The collapse rate of osteonecrosis of the femoral head was calculated during the follow-up. RESULTS AND CONCLUSION: (1) A total of 191 hips were included in this study. The femoral head collapsed in 86 hips during follow-up, with a total collapse rate of 45.0%. Among the influencing factors, age, ARCO stage and JIC classification were the main influencing factors of femoral head collapse (P < 0.05), but body mass index, sex, incidence side and pathogenic factors were not the main influencing factors (P > 0.05). (2) Among 191 hips, in JIC classification, the total collapse rates of type A, type B, type C1 and type C2 were 11.1%(2/18), 30.2%(16/53), 52.4%(43/82), and 65.8%(25/38), respectively. There were significant differences in the total collapse rate of the femoral head among all types (P < 0.05). The collapse risk results showed that the collapse risk of type B, type C1 and type C2 was 2.41, 5.22 and 7.89 times higher than that of type A, respectively. (3) Both JIC classification and ARCO stage were correlated with femoral head collapse (P < 0.01). There was no significant difference in the collapse rate of the femoral head among all JIC types in ARCO I stage hips (P > 0.05). In the hips with ARCO II stage, the collapse rates of the femoral head of JIC types A, B, C1 and C2 were 1.2%, 19.5%, 50.0% and 29.3%, respectively, and there were significant differences in the collapse rates among different types (P < 0.05). (4) During follow-up, the collapse rates of the femoral head in the first to fifth years were 29.3%, 7.9%, 4.7%, 2.6% and 0.5%, respectively. (5) Results showed that for early non-traumatic osteonecrosis of the femoral head, the risk of collapse of osteonecrosis of the femoral head is high within one year, and the location of the focus of osteonecrosis affects the risk of collapse of the femoral head. The effect of the location of the focus on the prognosis of the disease should be considered in clinical treatment. [ABSTRACT FROM AUTHOR]

Artemisia argyi is a kind of perennial plant of Artemisia in composite family, which contains volatile oils, flavonoids, triterpenes, eucalyptans and other active substances, and has biological functions such as antibacterial, antioxidant, digestive, improving immunity. As a common traditional Chinese herbal medicine in China, Artemisia argyi leaf has significant pharmacological functions. Products with multiple effects have been developed by using Artemisia argyi powder, Artemisia argyi stem and Artemisia argyi extract, and are widely used in animal husbandry as feed additives. The paper reviews the active components and biological functions of Artemisia argyi leavesan its application of Artemisia argyi leaves in livestock and poultry breeding and production, to provide reference for further development and utilization of Artemisia argyi leaves. [ABSTRACT FROM AUTHOR]

To solve the problem that there are fewer types of GSlVl-R mobile terminals so that specifications of China railway communication cannot be satisfierl, this paper proposes a low cost implementation scheme, where STM32 microprocessor is used as the main control unit, Siemens MC55i tri-band module as the communication module, and Texas Instruments TLV320AIC3204 audio codec chip as the audio module. The terminal realizes the cluster scheduling service function based on GSlVl-R network, and the call synchronization recording function requested by China Railway Corporation. Especially, the low power consumption design makes the terminal have better endurance ability. The test results of model machine show that the power consumption in standby mode is 36 mW, it can achieve more than 390 h standby time. [ABSTRACT FROM AUTHOR]

The residue and dissipation of 50 pesticides such as carbendazim, imidacloprid, and pyridaben during cucumber vines fermentation were determined by gas chromatography and ultra highperformance liquid chromatography-tandem mass spectrometry in view of the pesticide residue conditions of vegetable waste. The effect of compost heights on pesticide degradation was evaluated. The results shows that: nine pesticides, including procymidone, carbendazim, azoxystrobin, difenoconazole, diemthomorph, chlorfenapyr, imidacloprid, acetamiprid and pyridaben, were detected in the waste of vegetable. Carbendazim was found with highest average residue concentration of 11.2 mg/kg， and those of other pesticides ranged from 0.05 to 0.89 mg/kg. During the fermentation processes (24 days) under conditions with various compost heights, the concentrations of 9 pesticides degraded gradually and results of the dissipation of pesticides residues fitted to first-order kinetics. However，the half-lives varied between different pesticides. The longest half-life of 28.9 d was found in the case of imidacloprid. And the shortest half-life of 10.2 d was found in the case of carbendazim. The degradation of pesticides also varied with different compost heights. In general, the highest degradation rate and the shortest half-life were obtained using 2.5 m compost height. The results of this study provide insights into the parameter optimization and quality control during the vegetable waste fermentation process and a new strategy for the high efficiency utilization of agricultural wastes for the fertilizer preparation in the organic vegetable production. [ABSTRACT FROM AUTHOR]

Objective: Exploring the relationship between the amplitude of mismatch negativity(MMN) and the stimulus deviation in interaural phase differences (IPD) to find out the IPD with the greatest amplitude, and lay a theoretical foundation for improving the effect size of MMN. Methods: The average amplitude in Fz, FCz and Cz was obtained from 25 randomly selected college students and analyzed with repeated measurement analysis of variance. The stimuli was present by the rule of auditory oddball paradigm, in which the IPD of standard stimuli was 0° , and that of the deviant stimuli was 2.63° , 45° , 90° , 135° , 180° respectively. Results: There were 24 subjects left after outlier rejection. The main effect of stimuli deviation was significant (P＜ 0.01). The average amplitude of the IPD of 180° , 135° and 90° was significantly larger than that of 2.63° and 45° (P＜ 0.05) . But the difference between 2.63° and 45° was not statistically significant（P＞ 0.05）. The difference between 180° , 135° and 90° was not statistically significant again （P＞ 0.05）. Conclusion: The amplitude of IPD MMN mounts up with increasing stimuli deviation in the range of 0° to 90° , and then plateaus out. 180° is likely to be the optimal IPD of deviant stimulus for its greatest amplitude. [ABSTRACT FROM AUTHOR]

In this paper, the matrix effects on the residue analysis of 200 pesticides under gas chromatography-tandem mass spectrometry (GC-MS/MS) and ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) conditions were investigated. 17 different vegetable matrices based on the QuEChERS preparation were studied. The study is aimed to find the typical vegetables as blank matrices to compensate the matrix effects in pesticide residue analysis. The results showed that differences in matrix interferences and pesticides characteristics lead to varied matrix effects in and between GC-MS/MS and UPLC-MS/MS analysis. In GC-MS/MS analysis, the majority of the 150 pesticides exhibited matrix enhancement effects. The matrix effects of green onion, ginger and garlic were strong. Radish exhibited weak matrix effect. The matrix effects of the majority of vegetables could be well compensated by spinach, celery, cowpea and lettuce, and these four typical vegetables can be used in daily analysis as blank matrices. In the UPLC-MS/MS analysis, most of the 105 pesticides exhibited matrix depression effects. Garlic, ginger, green onion, leek, celery and spinach exhibited strong matrix effects and zucchini exhibited weak matrix effect. The matrix effects of different vegetables could be compensated by the four typical vegetables, including cucumber, Chinese cabbage, tomato and lettuce. However the compensation effects were less effective than that in the GC-MS/MS analysis. The results in this study have provided typical vegetables as blank matrices for reducing the matrix effects to obtain accurate results. [ABSTRACT FROM AUTHOR]

Objective: This study was designed to research the contributions of synovial cells whose Wnt/β-catenin signal pathway are activated to the cartilage cells, to discuss the ways and molecular mechanism of Osteoarthritis (OA). Methods: β-catenin over expressing lentivirus vector was constructed to transfect synovial cells of Human and fluorescence microscope was used to observe viral transfection efficiency. Expression of β-catenin protein of the synovial cells was detected by Western blot. Synovial cells and chondrocytes were cultured in transwell chamber and the influences of synovial cells to chondrocytes were observed under optical inverted microscope. Changes of MMP-7 in culture supernatant of chondrocytes was tested by Enzyme linked immunosorbent assay (ELISA) method at different time points to find the action time that synovial cells influence the chondrocytes best. Moreover, ELISA method was used to test CTX-II, COMP levels in the culture supernatant of chondrocytes at optimal time point. Results: After been transfected by lentivirus vector, fluorescent microscopy showed that most of the synovial cells were arise fluorescence. Expression of β-catenin increased significantly in the cytoplasm and nucleus of synovial cells (P<0.01). The growth morphology of chondrocytes did not change significantly under light microscope after co-cultured with synovial cells which Wnt/β-catenin signal pathway are activated. But chondrocytes' growth and adhesion force was reduced with the prolongation of the culture time, this may be related to the degradation of extracellular matrix. ELISA results showed that MMP-7 expression of chondrocytes supernatant was significantly increased, there was a large difference compared with the normal group (P<0.01). MMP-7 expression of chondrocytes culture supernatant were time-dependent. It increased gradually with the time prolonging at 2 h, 6 h and 24 h, but decreased slightly at 24 h. The expression of MMP-7 changed significantly after 6 hours'co-culture. ELISA results showed that the level of COMP, CTX-II in the supernatant of chondrocytes within singal pathway activated group was significantly higher than that in normal control group at 6 hours'co-culture (P<0. 01). Conclusions: β-catenin over expressing lentivirus can activate Wnt/β-catenin signaling pathway. The grouth of chondrocytes can be affected by synovial cells whose cause the abnormal of internal environment within synovial- cartilage system,promote cartilage matrix degradation at same time. This may be exist molecular interactions between two cells as a possible mechanism of synovitis promoting OA. [ABSTRACT FROM AUTHOR]

Objective: This study was designed to research the contributions of synovial cells whose Wnt/β-catenin signal pathway are activated to the cartilage cells, to discuss the ways and molecular mechanism of Osteoarthritis (OA). Methods: β-catenin over expressing lentivirus vector was constructed to transfect synovial cells of Human and fluorescence microscope was used to observe viral transfection efficiency. Expression of β-catenin protein of the synovial cells was detected by Western blot. Synovial cells and chondrocytes were cultured in transwell chamber and the influences of synovial cells to chondrocytes were observed under optical inverted microscope. Changes of MMP-7 in culture supernatant of chondrocytes was tested by Enzyme linked immunosorbent assay (ELISA) method at different time points to find the action time that synovial cells influence the chondrocytes best. Moreover, ELISA method was used to test CTX-Ⅱ, COMP levels in the culture supernatant of chondrocytes at optimal time point. Results: After been transfected by lentivirus vector, fluorescent microscopy showed that most of the synovial cells were arise fluorescence. Expression of β-catenin increased significantly in the cytoplasm and nucleus of synovial cells (P<0.01). The growth morphology of chondrocytes did not change significantly under light microscope after co-cultured with synovial cells which Wnt/β-catenin signal pathway are activated. But chondrocytes' growth and adhesion force was reduced with the prolongation of the culture time, this may be related to the degradation of extracellular matrix. ELISA results showed that MMP-7 expression of chondrocytes supernatant was significantly increased, there was a large difference compared with the normal group (P<0.01). MMP-7 expression of chondrocytes culture supernatant were time-dependent. It increased gradually with the time prolonging at 2 h, 6 h and 24 h, but decreased slightly at 24 h. The expression of MMP-7 changed significantly after 6 hours' co-culture. ELISA results showed that the level of COMP, CTX-Ⅱ in the supernatant of chondrocytes within singal pathway activated group was significantly higher than that in normal control group at 6 hours' co-culture(P<0.01). Conclusions: β-catenin over expressing lentivirus can activate Wnt/β-catenin signaling pathway. The growth of chondrocytes can be affected by synovial cells whose cause the abnormal of internal environment within synovial-cartilage system,promote cartilage matrix degradation at same time.This may be exist molecular interactions between two cells as a possible mechanism of synovitis promoting OA. [ABSTRACT FROM AUTHOR]

Objective: To explore the clinical efficacy of endoscopy and medication in treatment of patients with cirrhosis and esophageal variceal bleeding(EVB). Methods: A total of 70 patients with cirrhosis and EVB, who were treated in the Affiliated University-Town Hospital of Chongqing Medical University from January 2012 to August 2013, were selected and randomly divided into control group(n=35) and observation group(n=35). The two groups were treated with correcting anemia and expanding blood volume after admission. After the condition was stable, the observation group was treated with esophageal variceal ligation(EVL) under endoscopy in the case of pharyngeal anesthesia, while the control group was treated with somatostatin 3 mg+50 m L saline pumping and lansoprazole 40 mg+100 m L saline intravenous infusion. The clinical total effective rates, recurrence rates and the incidence of adverse reactions in the two groups were compared. Results: The total effective rate(91.43%) of observation group was significantly higher than that(71.43%) of control group(P<0.05); the bleeding recurrence rate(5.71%) in observation group was lower than that(22.86%) of control group(P<0.05); the incidence of esophageal ulcers, fever and retrosternal pain in the observation group were lower than those in the control group, but the differences had no significance(P<0.05). Conclusion: EVL is effective in the treatment of patients with cirrhosis and EVB, it can reduce the recurrence rate and the incidence of adverse reaction,and its effect is better than medication treatment,which is worthy of clinical promotion. [ABSTRACT FROM AUTHOR]