1. Monitoring Urinary Collagen Metabolite Changes Following Collagen Peptide Ingestion and Physical Activity Using ELISA With Anti–Active-Collagen-Oligopeptide Antibody
- Author
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Kaho Nomura, Yoshihiro Osawa, Hiroshi Mano, Aya Kataoka-Matsushita, Seiko Koizumi, Seiji Kushibe, Ken-ichi Takeyama, Masashi Nagao, and Yoshifumi Kimira
- Subjects
0301 basic medicine ,Adult ,Male ,Urinary system ,Metabolite ,Science ,Peptide ,Enzyme-Linked Immunosorbent Assay ,コラーゲン代謝物エライザ ,Pharmacology ,Antibodies ,Article ,collagen metabolite ELISA ,urinary ACOP ,03 medical and health sciences ,chemistry.chemical_compound ,Eating ,0302 clinical medicine ,Endocrinology ,バイオマーカー ,コラーゲン代謝 ,Ingestion ,Humans ,Exercise ,chemistry.chemical_classification ,Oligopeptide ,Multidisciplinary ,Active collagen oligopeptides (ACOP) ,biology ,活性型コラーゲンオリゴペプチド(エーコップ) ,Chemistry ,尿中エーコップ ,Metabolism ,Middle Aged ,collagen turnover ,030104 developmental biology ,030220 oncology & carcinogenesis ,biology.protein ,Biomarker (medicine) ,Medicine ,biomarker ,Collagen ,Antibody ,Oligopeptides ,Biomarkers - Abstract
Active collagen oligopeptides (ACOP) are bioactive collagen-derived peptides detected by a recently-established ELISA. To facilitate studies of the function and metabolism of these products, this study aims to determine which of these peptides is recognized by a novel anti-ACOP antibody used in this ELISA. We then investigate the effect of collagen peptide (CP) ingestion and exercise on urinary ACOP concentrations in a cohort of university student athletes using colorimetric, LC–MS/MS, and ELISA. We observed that the antibody showed strong cross-reactivity to Pro-Hyp and Gly-Pro-Hyp and weak cross-reactivity to commercial CP. CP ingestion increased the urinary level of ACOP over time, which correlated highly with urinary levels of peptide forms of Hyp and Pro-Hyp. Physical activity significantly decreased the urinary ACOP level. This study demonstrates changes in urinary ACOP following oral CP intake and physical activity using ELISA with the novel anti-ACOP antibody. Thus, ACOP may be useful as a new biomarker for collagen metabolism., Article number: 13527 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http:// creat iveco mmons. org/ licen ses/ by/4. 0/.
- Published
- 2021