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Monitoring Urinary Collagen Metabolite Changes Following Collagen Peptide Ingestion and Physical Activity Using ELISA With Anti–Active-Collagen-Oligopeptide Antibody
- Source :
- Scientific Reports, Scientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
- Publication Year :
- 2021
- Publisher :
- Nature Research, 2021.
-
Abstract
- Active collagen oligopeptides (ACOP) are bioactive collagen-derived peptides detected by a recently-established ELISA. To facilitate studies of the function and metabolism of these products, this study aims to determine which of these peptides is recognized by a novel anti-ACOP antibody used in this ELISA. We then investigate the effect of collagen peptide (CP) ingestion and exercise on urinary ACOP concentrations in a cohort of university student athletes using colorimetric, LC–MS/MS, and ELISA. We observed that the antibody showed strong cross-reactivity to Pro-Hyp and Gly-Pro-Hyp and weak cross-reactivity to commercial CP. CP ingestion increased the urinary level of ACOP over time, which correlated highly with urinary levels of peptide forms of Hyp and Pro-Hyp. Physical activity significantly decreased the urinary ACOP level. This study demonstrates changes in urinary ACOP following oral CP intake and physical activity using ELISA with the novel anti-ACOP antibody. Thus, ACOP may be useful as a new biomarker for collagen metabolism.<br />Article number: 13527 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http:// creat iveco mmons. org/ licen ses/ by/4. 0/.
- Subjects :
- 0301 basic medicine
Adult
Male
Urinary system
Metabolite
Science
Peptide
Enzyme-Linked Immunosorbent Assay
コラーゲン代謝物エライザ
Pharmacology
Antibodies
Article
collagen metabolite ELISA
urinary ACOP
03 medical and health sciences
chemistry.chemical_compound
Eating
0302 clinical medicine
Endocrinology
バイオマーカー
コラーゲン代謝
Ingestion
Humans
Exercise
chemistry.chemical_classification
Oligopeptide
Multidisciplinary
Active collagen oligopeptides (ACOP)
biology
活性型コラーゲンオリゴペプチド(エーコップ)
Chemistry
尿中エーコップ
Metabolism
Middle Aged
collagen turnover
030104 developmental biology
030220 oncology & carcinogenesis
biology.protein
Biomarker (medicine)
Medicine
biomarker
Collagen
Antibody
Oligopeptides
Biomarkers
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 11
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....0b7d79e46c68ac40a6d6c73d11cf414f