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目的应用超声心动图多参数评估特发性室颤患者的超声表型特征，为心律失常高危患者的早期预警提供超声影像学的支持。方法纳入2019年1月至2023年12月在中山大学孙逸仙纪念医院首次诊断为特发性心室颤动（IVF）的患者，同时纳入年龄和性别匹配的健康个体作为对照。基于二维灰阶、M型、双平面、频谱多普勒、组织多普勒、斑点追踪、压力-应变环等技术，对纳入人群进行超声心动图多参数分析。超声指标包括传统参数、心肌机械运动参数、心肌电-机械活动参数以及心肌做功参数。使用t检验或Mann-Whitney U检验进行两组独立样本之间的比较。进行受试者工作特征（ROC）曲线分析，以评估超声心动图多参数对特发性室颤的诊断价值。使用组内相关系数（ICC）评估各超声参数观察者内部和观察者之间的变异性。结果本研究共纳入IVF患者9例，健康对照个体30例。共计702个左室节段和117个右室游离壁节段纳入心肌功能的分析。与健康对照组相比，IVF组左室整体纵向应变（LV-GLS）相对较低【（18.8±2.7）% vs.（21.2±1.9）%，P=0.004】，而左室机械离散度（LV-MD）相对较高【（44±13）ms vs.（36±9）ms，P=0.022】。心肌做功结果显示，IVF组的整体做功指数（GWI）低于健康对照组【（1991±365）mmHg% vs.（2319±408）mmHg%，P=0.037】。ROC曲线显示LV-GLS、LV-MD以及GWI对特发性室颤的诊断性能较好，曲线下面积（AUC）分别为0.748、0.737以及0.722，截断值分别为19.5%、39.5ms以及2049mmHg%。上述三项指标组成的综合诊断体系，其AUC为0.800。超声心动图各参数在观察者内部和观察者之间均呈现出较好的可重复性，ICC值均大于0.75。结论特发性室颤患者超声心动图多参数表现出不同程度的左室功能异常。LV-GLS降低、LV-MD增加以及GWI降低，是恶性心律失常高危的超声心动图特征。

目的本研究通过测定心脏淀粉样变（CA）患者与高血压左室肥厚（HLVH）患者的左室长轴心肌应变相关指标，并比较其差异性，进一步明确左室长轴心肌应变在CA患者早期诊断的价值。方法选出确诊为CA的患者41例，按患者既往是否合并原发性高血压分为CA并高血压组14例与单纯CA组27例，同时按性别、年龄及体表面积匹配HLVH患者20例及健康对照组20例。收集患者的临床资料，获取常规超声心动图指标包括室壁厚度（IVST、LVPWT、RWT）、心腔大小（LVEDD、LAVI）、收缩及舒张功能指标（LVEF、平均E/e'），以及左室长轴应变相关指标（GLS、RELAPS、EFSR）。比较4组研究对象的组内差异并进行组间两两比较，进一步测定LVEF保留时各超声心动图指标预测CA的敏感性与特异性。结果①超声心动图的常规指标中CA并高血压组和单纯CA组的LVEDD和LVEF均低于HLVH组；而CA并高血压组和单纯CA组的LVPWT、RWT及平均E/e'均高于HLVH组（P均＜0.05）。②应变指标中CA并高血压组和单纯CA组的GLS绝对值最低，HLVH组次之，健康对照组最高；同时，CA并高血压组和单纯CA组的RELAPS与EFSR值最高，HLVH次之，健康对照组最低（P均＜0.05）。CA并高血压组和单纯CA组的所有超声心动图指标差异均无统计学意义（P均＞0.05）。③ROC曲线分析显示在LVEF保留时，GLS绝对值＜14.35%或EFSR＞4.28时可较好地鉴别CA和HLVH（AUC均＞0.9，P均＜0.05）；GLS诊断的敏感性最高（100%），而EFSR诊断的特异性最高（95%）。结论无论CA是否合并高血压，与HLVH相比，其超声心动图的常规及应变指标上均有显著的差异；尤其是应变指标GLS和EFSR在左室肥厚并LVEF保留患者中预测CA的准确性更高。

伴随着数字经济的快速发展，品牌传播有利于提升数字品牌影响力，助力企业数字转型。基于交互情感评估地标产品品牌传播效果，能够助力县级政府更好地打造地标产品品牌。首先，提出基于交互情感的品牌数字化传播评估模型，发现并解决现有地标产品品牌数字化传播体系化缺失的问题，以系统化动力学机制提升数字品牌影响力；然后以浙江省地标产品为背景，采用微博数据，分析了地标产品品牌传播效果层级模型的可用性。实际应用结果表明，提出的基于交互情感的品牌数字化传播评估模型具备指引地标产品品牌数字化建设的普适性。

目的探讨器质性二尖瓣脱垂合并瓣叶重度关闭不全患者的二尖瓣瓣环随心动周期变化的形态特征，为术前评估脱垂的二尖瓣提供更多准确信息。方法使用配有经食管三维探头X7-2t的Philips Epic7C 超声心动图仪以及QLAB的MVN软件，收集并分析18例二尖瓣脱垂并重度关闭不全患者和36例正常二尖瓣患者的二尖瓣瓣环相关参数。结果各个时相二尖瓣脱垂合并重度关闭不全组患者的二尖瓣瓣环前外侧与后内侧直径、前后径、瓣环周长及瓣环面积均大于正常对照组（PPP>0.05）。结论二尖瓣瓣环的立体形态和功能参数随着心动周期不同时相而发生动态改变，三维食道超声技术为器质性二尖瓣脱垂提供了准确详尽的信息。

目的研究血管平滑肌自噬对小鼠主动脉夹层形成的影响。方法①构建小鼠主动脉夹层模型：60只C57BL/6小鼠被随机分为三组：阴性对照组、实验（AD）组、氯喹（CQ）干预组，实验组与CQ组使用β-氨基丙腈（BAPN）喂养联合人血管紧张素-Ⅱ（Ang-Ⅱ）皮下注射16 d构建小鼠主动脉夹层的模型，同时干预组每天腹腔注射CQ，对照组给予生理盐水。②Yes相关蛋白（YAP）、自噬相关蛋白及血管平滑肌细胞相关标志蛋白的检测：采用蛋白质免疫印迹法、免疫组织化学染色法对小鼠主动脉样本中YAP、微管相关蛋白1轻链3（LC3）、P62及血管平滑肌细胞不同表型相关标志蛋白进行检测。结果①实验组主动脉夹层的发生率为60%，干预组小鼠未见明显主动脉夹层形成征象。②与对照组相比，实验组与干预组小鼠主动脉中合成型血管平滑肌细胞（VSMC）标志蛋白骨桥蛋白（OPN）、LC3含量明显增高，同时，收缩型VSMC标志蛋白α-SMA及YAP蛋白的表达下降（P

【目的】运用心脏三维全自动左心定量技术（HM）评价心脏起搏器植入患者的左心室容积及射血分数。【方法】对 50 例心脏起搏器植入患者行超声心动图检查，采集其二维及三维图像，采用双平面Simpson 法、HM 法及传统三维手动测量（3D-manual）法分别测出左心室舒张末容积（LVEDV）、左心室收缩末容积（LVESV）、左室射血分数（LVEF）。对三种方法的测量结果及后处理时间进行比较。同时评价 HM 方法组内及组间的重复性。【结果】Simpson、HM、3D-manual 法测量 LVEDV 和 LVEF 值总体差异均有统计学意义（均 P0.05）。HM 与 3D-manual 的测值有极高的相关性和更好的一致性。HM 法的后处理时间最少，且观察者内和观察者间的重复性高。【结论】HM 法自动评价心脏起搏器植入患者左心室容积及射血分数快速、可行，且具有高度重复性。

[目的]观察一个肢带型肌营养不良伴心肌损害家系患者的临床病理特点。[方法]行家系调查,家系成员行心肌酶及肌钙蛋白T、心电图、肌电图、彩超检查;4位患者取肌肉标本进行组织化学的病理观察。[结果]系谱显示4代共有9位患者,每代都出现,男女均受累及。健在5位患者,均出现进行性肌肉无力、萎缩,以肩带及盆带部明显;心肌酶及肌钙蛋白T检查正常;部分患者出现心脏扩大、心律失常、心力衰竭。病理检查见明显肌萎缩、核内移、肌裂等现象,符合肌营养不良的典型表现,Dystrophin免疫组化结果为（＋）。[结论]该家系符合常染色体显性遗传的特点;根据患者临床及病理特点,诊断为肢带型肌营养不良症伴心肌损害,以肢带型肌营养不良症ⅠB型（LGMD-ⅠB）可能性大;进一步明确其基因分型需行分子遗传学检查。

【目的】观察不同剂量血管紧张素-（1-7）对慢性心力衰竭大鼠心室有效不应期及心室颤动阈值的影响。【方法】40只Wistar大鼠成功建立慢性心力衰竭模型，随机分为心力衰竭组（n=10）、心力衰竭并血管紧张素-（1-7）处理3个剂量组（每组n=10）。另取10只Wistar大鼠行假手术，为正常对照组。用心电生理研究方法测定心室有效不应期（VERP）、心室颤动阈值（VFT）的变化，比较心力衰竭并血管紧张素-（1-7）处理的3个剂量组、心力衰竭组及正常对照组各项指标。【结果】心力衰竭大鼠VERP（73．5ms±2．4ms）较正常对照VERP（60．0ms±4．1ms）延长，VFT（6．7mA±1．4mA）较正常对照（30．4mA±1．3mA）降低。血管紧张素-（1-7）5μg·kg^-1处理心力衰竭大鼠VERP及VFT较心力衰竭大鼠无显著性差异（P〉0．05）。血管紧张素-（1-7）15μg·k^-1处理心力衰竭大鼠VERP（63．0ms±2．6ms）较心力衰竭大鼠VERP（73．5ms±2．4ms）缩短，VFT（17．1mA±3．1mA）较心力衰竭大鼠（6．7mA±1．4mA）增高。血管紧张素-（1-7）25μg·k^-1处理心力衰竭大鼠VERP（62．5ms±2．6ms）较心力衰竭大鼠VERP（73．5ms±2．4ms）缩短，VFr（19．0mA±2．4mA）较心力衰竭大鼠（6．7mA±1．4mA）增高，且较血管紧张素-（1-7）15μg·k^-1。处理心力衰竭大鼠VFT（17．1mA±3．1mA）增高。相关分析显示：VERP与VFT负相关：血管紧张素-（1-7）的3个不同处理剂量与VERP负相关，与VFT正相关。【结论】血管紧张素-（1-7）能改善心力衰竭大鼠心电生理不稳定状态，提高心室颤动阈值，并具有剂量效应关系。

【目的】观察充血性心力衰竭大鼠肺组织水通道蛋白(Aquaporins, AQP)表达的变化及选择性内皮素受体A(endo thelin recepto r A , ETA)拮抗剂FR139317 对其的影响。【方法】40 只行左冠状动脉结扎致心肌梗死的大鼠随机分为心衰组 和心衰治疗组, 心衰治疗组予以FR139317 每天1 mg/kg 皮下注射, 共6 周, 另7 只接受假手术的大鼠组成空白对照组。测定 血液动力学参数, 放射免疫法(RIA)测血浆内皮素-1 浓度, 逆转录-聚合酶链反应(RT-PCR)法定量检测肺组织AQP4 和AQP5 m RNA 的表达。【结果】空白对照组、心衰组和心衰治疗组肺组织AQP4 m RNA 的相对水平为(0.56 ±0.12)、(0.20 ±0.08)及 (0.36 ±0.14), P

【目的】观察支架植入后对血管中膜平滑肌细胞增殖和凋亡的影响, 探讨支架植入后再狭窄的发生机制。【方 法】将雄性新西兰兔50 只随机分为球囊组和支架组, 设正常对照。分别于术后第3 、7、14 、28、56 天取材研究:①用免疫组化 检测血管中膜平滑肌细胞中增殖细胞核抗原(PCNA)和细胞周期蛋白E(Cy clin E)的表达;②用TUNEL 染色法测定血管中膜 平滑肌细胞的凋亡。【结果】与对照组比较, 支架组和球囊组PCNA 、Cyclin E 表达及细胞凋亡均有显著增加。支架组与球囊 组比较, ①PCNA 和Cy clin E 表达明显增加, 术后第7 天, 支架组和球囊组PCNA 阳性率为24.36% ±0.55% vs 18.74 %± 1.09% (P

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Interlayer characterization in sparse well area of offshore oilfield is a difficult problem in reservoir characterization. Taking the H3 sand group of X oilfield in Xihu sag, East China Sea Basin as an example. Based on the analysis of the genetic type of the interlayer, the identification standard of the electrical characteristics of the interlayer was established, and the spatial distribution of the interlayer was semi-quantitatively characterized by the method of geological and seismic coupling. The results show that the H3 sand group develops six interlayers: flood type, abandoned channel type, silt type, channel retention type, fine-grained sedimentary type and diagenesis type. According to the permeability of the interlayer, the flood type, the abandoned channel type and the silt fall are classified as the muddy interlayer, the channel retention type and the fine-grained sedimentary type are classified as the physical interlayer, and the genetic type is classified as the calcareous interlayer. The interlayer between H3a and H3b in H3 sand group is very developed, with large thickness, good continuity, stable distribution in the whole area and good sealing effect. the interlayer between H3b and H3c is unevenly developed, and the thickness is thin in the north of X10, west of X7-X8, X4-X5 area and the southwest of X1, resulting in weak connectivity between H3b and H3c sand bodies. The research results better characterize the distribution of interlayers in the H3 sand group, and provide a geological basis for remaining oil analysis. The method of geological and seismic coupling can effectively describe the interlayers in the thin well pattern area of offshore oilfield, which has a good reference for the fine characterization of reservoirs in similar areas. [ABSTRACT FROM AUTHOR]

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BACKGROUND: Additive manufacturing technology provides strong technical support for personalized and precise medical treatment due to its rapid prototyping, digitalization, and personalization. Therefore, its medical devices have developed rapidly in orthopedics, oral, preoperative planning, craniomaxillofacial, and other clinical applications. OBJECTIVE: To briefly introduce the progress of additive manufacturing technology and its commercial application in the clinic in and outside China. METHODS: Wanfang Data, China National Knowledge Infrastructure (CNKI), PubMed, Elsevier, Springer Link databases, and the websites of National Medical Products Administration and FDA were searched using “additive manufacturing, medical device, clinical application, 3D printing” as Chinese search terms and “additive manufacturing (AM), additive manufacturing technology, medical device, clinical application, 3D printing” as English search terms. The search time ranged from January 2010 to March 2023, and a few classical long-term articles were included. RESULTS AND CONCLUSION: Additive manufacturing technology is classified by the form of raw materials (liquid-based, solid-based, and powder-based). At present, there are seven kinds of additive manufacturing processes, including fused deposition molding, stereo-curing molding, powder bed melting, directed energy deposition, binder jet 3D printing, inkjet printing, and layered solid manufacturing. The marketed additive manufacturing products include orthopedic products, dental products, preoperative planning guide plate/bone model products, and craniomaxillofacial products. The orthopedic products include matching long-segment bone defect restoration, interbody fusion cage, knee joint orthosis, vertebral prosthesis, thoracolumbar fusion matching prosthesis system, knee prosthesis, and hip prosthesis. Dental products include light-curing resin for additive manufacturing and BB Base 3D Printing Resin for Denture Base. The products of preoperative planning guide plates/bone models include bone models, medical orthopedic surgical guide plates, customized dental implant guide plates, and human organ models. The craniomaxillofacial products include polycaprolactone scaffolds, patient-specific skull implants, OsteoFab patient-specific face device, SpinFab VBR prosthesis, and TruMatch CMF titanium alloy 3D printing prosthesis system. [ABSTRACT FROM AUTHOR]

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