Search

【Objective】The aim of this paper is to analyze the genetic diversity of faba bean germplasm resources by transcriptome sequencing and SSR molecular marker technology， and to explore the molecular markers associated with bruchid resistance traits in faba bean. So as to provide a reference basis for the conservation and utilization of bruchid resistance germplasm resources and the selection and breeding of new varieties. 【Method】Transcriptome sequencing of two faba bean varieties Y134 and Y078 was performed by PacBio triple full-length sequencing technology combined with RNA-seq.. Based on this， all Unigene of the transcriptome were searched for Unigene containing SSR core motifs using the software MISA， and primers were designed in batch using Primer 3.0. One hundred pairs of SSR primers were randomly selected for genetic diversity analysis of 80 faba bean germplasm resources. 【Result】Fifty of the 100 SSR primer pairs amplified products， with an effective amplification rate of 50%; 32 of the primer pairs showed polymorphism. They accounted for 64% of the effective products and 32% of the total primers. Among the 32 polymorphic primer pairs， each pair amplified 2 to 21 alleles with an average of 6.875; The polymorphic information content （PIC） values ranged from 0.25 to 0.82 with an average of 0.58; The Shannon’s information index varied from 0.34 to 2.31 with an average of 1.21. Based on UPGMA cluster analysis， the 80 faba bean materials were classified into two major groups at a similarity coefficient of 0.70. The first category contained 65 germplasm， including 62 highly resistant （HR） varieties， 2 highly sensitive （HS） varieties and 1 sensitive （S） variety; The second category contained 15 germplasm， 7 highly susceptible （HS） varieties， 2 sensitive （S） varieties， 5 highly resistant （HR） varieties and 1 moderately resistant （MR） variety. 【Conclusion】The SSR markers developed were used to analyze the genetic diversity of 80 faba bean germplasm resources and to lay the theoretical foundation for research on the identification of faba bean germplasm resources resistant to bean weevil and molecular marker-assisted selection for breeding. [ABSTRACT FROM AUTHOR]

【Objective】The present paper aimed to identify the augophagy(ATG) gene of Vicia faba L. based on the results of transcriptional sequencing, and analyze its response to drought stress, which provides theoretical reference for further study on the mechanism of these genes under drought stress.【Method】According to the annotation of Swiss port, NCBI,ensemble and other databases, the ATG gene of V. faba L. was screened and identified based on RNA sewuencing data. The conserved domain, subcellular localization prediction, phylogenetic tree and motif were analyzed by SMART,MEGA-X and MEME software. Based on transcriptome data, the expression differences of ATG gene in V. faba L. varieties CDAS105(drought tolerant) and ECAN No.1(sensitive) were analyzed under drought stress.【Result】 Twelve ATG genes from V.faba L. were screened and annotated, all of which contained autophagy domain. According to the different C-terminal domains, they were divided into five subfamilies: ATG3,ATG6,ATG8,ATG18 and ATG26. In the phylogenetic tree constructed by faba bean ATG gene and Arabidopsis,maize, rice and yeast, it was found that genes with the same C-terminal domain were clustered together. Prediction of subcellular localization indicated the elevent genes and one ATG gene were located in extracellular and cytoplasm, respectively. Gene structure analysis showed that there were no introns in the DNA sequences of ATG family genes in V. faba L.,and they were composed of UTR and CDs regions. Conserved motif analysis identified that these proteins contained 20 mortifs. Analysis of ATG expression showed that ATG18 b,ATG18 c and ATG8 a were up-regulated in drought resistant varieties after 16 hours or 64 hours drought treatment, and the expression of ATG8 a in drought resistant varieties was further enhanced with the increase of drought treatment time. 【Conclusion】Twelve ATG genes were identified and distributed in five subfamilies. ATG8 a, ATG18 b and ATG18 c responded to drought stress, which could be used as potential gene resources to improve the drought tolerance during seed germinating in faba bean. [ABSTRACT FROM AUTHOR]