蚕豆自噬基因鉴定及响应干旱胁迫分析.

【Objective】The present paper aimed to identify the augophagy(ATG) gene of Vicia faba L. based on the results of transcriptional sequencing, and analyze its response to drought stress, which provides theoretical reference for further study on the mechanism of these genes under drought stress.【Method】According to the annotation of Swiss port, NCBI,ensemble and other databases, the ATG gene of V. faba L. was screened and identified based on RNA sewuencing data. The conserved domain, subcellular localization prediction, phylogenetic tree and motif were analyzed by SMART,MEGA-X and MEME software. Based on transcriptome data, the expression differences of ATG gene in V. faba L. varieties CDAS105(drought tolerant) and ECAN No.1(sensitive) were analyzed under drought stress.【Result】 Twelve ATG genes from V.faba L. were screened and annotated, all of which contained autophagy domain. According to the different C-terminal domains, they were divided into five subfamilies: ATG3,ATG6,ATG8,ATG18 and ATG26. In the phylogenetic tree constructed by faba bean ATG gene and Arabidopsis,maize, rice and yeast, it was found that genes with the same C-terminal domain were clustered together. Prediction of subcellular localization indicated the elevent genes and one ATG gene were located in extracellular and cytoplasm, respectively. Gene structure analysis showed that there were no introns in the DNA sequences of ATG family genes in V. faba L.,and they were composed of UTR and CDs regions. Conserved motif analysis identified that these proteins contained 20 mortifs. Analysis of ATG expression showed that ATG18 b,ATG18 c and ATG8 a were up-regulated in drought resistant varieties after 16 hours or 64 hours drought treatment, and the expression of ATG8 a in drought resistant varieties was further enhanced with the increase of drought treatment time. 【Conclusion】Twelve ATG genes were identified and distributed in five subfamilies. ATG8 a, ATG18 b and ATG18 c responded to drought stress, which could be used as potential gene resources to improve the drought tolerance during seed germinating in faba bean. [ABSTRACT FROM AUTHOR]