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目的：研究CD4 T淋巴细胞内的基础ATP浓度代替CD4/CD8比值用于评估结核病感染者免疫状态的可行性。方法：本研究通过磁珠法分离相同体积全血中的CD4、CD8细胞，并计算CD4和CD8细胞的基础ATP浓度来评估结核病感染者的免疫状态，以QFM（Quantiferon Monitor）检测法作为对照。结果：研究发现传统的CD4/CD8比值或CD4 T细胞计数方法不适用于结核病患者。采用CD4细胞的基础ATP浓度来监测活动性结核患者和健康人群，在以290.7×10-6 g/L为cut-off值，AUROC为0.8719，其灵敏度为86.21%，特异性为76.19%，差异有统计学意义（P+T淋巴细胞胞内基础ATP浓度来评估结核病感染者的免疫状态。提示CD4+ T淋巴细胞的基础ATP浓度比CD4/CD8比值可以更好用于评估结核病患者的免疫状态。

兴凯湖保护区是我国东北穆棱-三江平原湿地最具代表性、保存最好、面积最大的区域,而且与俄罗斯接壤, 可以说是湿地生物多样性保护的一个热点区域。在当前提倡对湿地、淡水水域和海域加大保护力度的关键时刻,集中相应的人力、物力对它给予更多的关注是十分必要的。本文对其基本特点、有效管理的成就和存在的问题作简要介绍,并提出相应的建议,供有关方面参考。

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