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【Objective】To analyze genetic diversity of wild Cymbidium goeringii germplasm resources in Leye County, Guangxi and to construct DNA fingerprint of the plant, so as to provide a scientific basis for the classification and identification of wild C. goeringii germplasm resources. 【Method】Percentage of polymorphic loci (PPL), polymorphism information content (PIC), observed number of alleles (Na), effective number of alleles (Ne), the Nei's gene diversity (He) and Shannon's information index (I) of 81 samples of 4 wild C. goeringii cultivars in Leye County, Guangxi were analyzed by iPBS markers. Primers which were clear, of high polymorphism and good reproducibility were screened from 83 iPBS primers for PCR amplification of 85 C. goeringii germplasms and gel electrophoresis characteristic bands were recorded. POPGEN 1.32 software was used to analyze each genetic diversity index. UPGMA cluster analysis was conducted based on their genetic similarity coefficient. Digital fingerprint of C. goeringii germplasm was constructed according to polymorphism sites of primer amplification band. 【Result】Through amplification of 6 primer, 105 clear bands were obtained, in which 95 bands were polymorphic, accounting for 90.48%. The average values of PIC, Na, Ne, He were I 0.8050, 1.7923, 1.2204, 0.2765 and 0.4590, respectively. The genetic similarity coefficient among the germplasms ranged from 0.690 to 0.981. When genetic similarity coefficient was 0.70485, germplasms could be divided into 4 groups, of which 79 germplasms of Leye were mainly distributed in group Ⅰ, 4 cultivars were distributed in group Ⅱ, while only one specie from Leye was distributed in groups Ⅲ and Ⅳ respectively. The digital fingerprint map constructing showed that, 85 C. goeringii accessions were fully distinguished using the combined polymorphic sites amplified with 2 pairs of iPBS primers (2249 and 2076), thus the digital fingerprint was constructed, which could provide a scientific basis for the identification of wild C. goeringii germplasms. 【Conclusion】Great genetic diversity was found among 81 C. goeringii germplasms in Guangxi with frequent gene communication and similar genetic conditions. iPBS can be applied for C. goeringii germplasm identification and genetic polymorphism. [ABSTRACT FROM AUTHOR]

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[Objective] In order to use Phalaenopsis ge mi plasm resourees and perfeet the systematic classification of Phalaenopsis, stomal al characteristics were studied, then the indexes were evaluated by correlation analysis and cluster analysis of Phalaenopsis leaves. [MethodJ 25 cultivars of Phalaenopsis were used as test materials, their 13 stomatal characteristics index (The number of upper epidermis cells, the stomatal number of upper epidermis, the number of lower epidermis cells, the stomatal number of lower epidermis, the stomatal density, the stomatal index, the outer longitudinal diameter, the outer transveise diameter, the inner longitudinal diameter, the inner transveise diameter, the stomatal opening, the stomatal area and the stomatal ratio) were measured and analyzed. [Result] (i) The cell shape of the upper and lower epidermis of the Phalaenopsis leaf was polygonal irregular, and the arrangement of stomata was irregular. (ii) There were significant differences in the stomatal charactal of Phalaenopsis. The stomatal characteristics of 20 Phalaenopsis cultivais were more than that of the lower epidermis, accounting for 80% of the total. No stomatal apparatus was found in the lower epidermis of R7. The outer longitudinal diameter, inner longitudinal diameter, inner transverse diameter, stomatal opening, stomatal area and stomatal ratio of R7 were the smallest a-mong the tested varieties, which were 18.48 mm, 8.3 mm, 9.99 mm, 84.6, 375.92 mm2 and 0.72, respectively. From 0.72 to 1.35, the shape of stomatal oigans of Phalaenopsis species was mainly round or oblate, which was consistent w ith the visual observation. (iii) Correlation analysis showed that there was a high correlation between stomatal indexes of the 13 leaves. (hr) Through the principal component analysis, 13 stomatal characteristics could be synthesized into4 principal components, and the cumulative contribution rate of the fiist four principal components was 91.062%. (v) The result of cluster analvsis showed that 25 Phalaawpsis could be divided into 3 categories at Euclidean distance of 15, category I included 2 species of large flower with purple-red lines, cat eg or)-1(included 1 specie of small flower with purple-red lines, and category III included 22 species of flower with unline or unpuiple-red lines. [Conclusion] The stomatal characteristics could be used as one of the criteria for classification of Phaloenopsis. [ABSTRACT FROM AUTHOR]

Copyright of Southwest China Journal of Agricultural Sciences is the property of Editorial Department of Southwest China Journal of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Guihaia is the property of Guihaia Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Southwest China Journal of Agricultural Sciences is the property of Editorial Department of Southwest China Journal of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Southwest China Journal of Agricultural Sciences is the property of Editorial Department of Southwest China Journal of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In order to reduce the production cost and short the period of Phalaenopsis spp. seedlings，using technology of rooting out of testtube ，the effect of different varieties，different culture stage，different kind and concentration of hormones were studied on rooting out of testtube. The results showed that different varieties of rooting out of test-tube was different. The effects of 3 varieties of Phalaenopsis spp. which rooted out of test-tube were ranked ： ‘ Liulin black rose ' > ' Wenjing Angel ' > ‘ pepper ' ； tissue cultured seedlings which after strong seedling culture was suitable for rooting out of test-tube . With 100 mg/L IBA solution treatment，outside the bottle rooting effect was the best， the rooting rate was 94. 80 %. The average root number was 3. 72 and the average number of leaves was 4. 24， the rooting time was 38. 00 days. With 100 mg/L N A A solution treatment，bottle rooting had better effect，the rooting rate was 86. 80 % ，average root number was 3. 84，average leaf number was 3. 30，rooting time was 36. 40 days. [ABSTRACT FROM AUTHOR]

Genetic variation characteristics and important quantitative traits of phalaenopsis flower diameter were studied to provide reicrences for rationally evaluating, exploring and utilizing the existing germplasm resources. IS varieties of Phalaenopsis were used as materials. 10 quantitative traits and flower diameter were measured. The data were statistically processed using correlation, path, and stepwise regression analyses , and multivariate regression equations were calculated. The results were as follows. (i) The correlation coefficients were difierent between Phalaenopsis flower diameter and each quantitative trait, and the correlation coefficient of nr3 (sepal length),( sepal width) , *7 (petal length) and % (petal width) was over 0.9. (ii)The path coefficients of %s for flower length were the largest, 1.805, and was significant different ({ =0. 05) ; (iii) The multivariate regression equation was j\ — 3. 685 t 2. 546ut5 for flower length, and -4. 884 t 0. 395yj +0.017*I +0.062 x2 +0.074 -0.03 -r4 +0.45 x5 -0. 14 x6 -0.252 r7 +0.65 r8 +0.392 x9 -1.067 vLf} for flower width. It can be concluded that the highest correlation is between phalaenopsis flower diameter and sepal length. Sepal length is the main factor affecting flower diameter, so sepal length can be used as the top breeding parameter for increasing flower diameter. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Southern Agriculture is the property of Journal of Southern Agriculture and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Southwest China Journal of Agricultural Sciences is the property of Editorial Department of Southwest China Journal of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Southern Agriculture is the property of Journal of Southern Agriculture and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)