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Messenger RNA(mRNA)delivery technology refers to the synthesis of mRNA by in vitro transcription and delivery to cells, and the expression of corresponding proteins to exert important biological functions.In recent years, mRNA delivery technology has become a research frontier and hotspot in vaccine and gene therapy.mRNA is generally packaged with nanoparticles or non-nanoparticles for delivery.Despite many advances, the lack of effective and non-toxic delivery methods remains a major factor limiting the application of mRNA.The use of viral systems(such as lentiviruses and adeno-associated viruses)has been widely recognized as an effective way to deliver nucleic acids, but an unwanted immune response is a fundamental barrier to its development.As a result, more and more non-viral vectors(including lipids, polymers, nano-based carriers or functional carriers)have attracted attention as safe and effective delivery vehicles.This paper reviewed the progress, challenges and future directions of various mRNA delivery materials in recent years. [ABSTRACT FROM AUTHOR]

PX domain containing serine/threonine kinase (PXK) is a multitissue, broad-spectrum expressed gene that plays an important role in immune-related diseases. The objective of this study was to analyze the relationship between PXK and the development of acute myelocytic leukemia (AML) by using bioinformatics methods in multiple databases. The UALCAN database was utilized to conduct an online analysis of PXK expression in various subtypes of AML. LinkedOmics was utilized to analyze the correlation between the expression level of PXK and the clinicopathologic features of AML. The Kaplan-Meier Plotter database was used to analyze the mRNA expression of PXK in AML and to evaluate the effect of PXK on survival in AML. PXK interacting protein network and expression correlation were analyzed by String and GEPIA databases. The correlation between PXK gene and tumor infiltrating immune cells was analyzed by quanTIseq algorithm, and" xCell" R package was used to analyze the correlation between PXK gene expression and multiple immune checkpoints. PXK and its co-expressed genes were functionally enriched in the Metascape database to anticipate their biological roles. The findings revealed that PXK was highly expressed in various cancer tumor tissues when compared to normal tissues, and the expression level of PXK in AML was significantly up-regulated, which had an effect on the clinicopathological features, survival rate, and prognosis of AML patients, whereas the prognosis of AML patients with high PXK expression was better. PXK is highly expressed in AML tissues and is closely related to the initiation and progression of AML, which may be one of the important indicators for the diagnosis, treatment and prognosis evaluation of AML. [ABSTRACT FROM AUTHOR]