体外转录合成 mRNA 递送材料的研究进展.

Messenger RNA(mRNA)delivery technology refers to the synthesis of mRNA by in vitro transcription and delivery to cells, and the expression of corresponding proteins to exert important biological functions.In recent years, mRNA delivery technology has become a research frontier and hotspot in vaccine and gene therapy.mRNA is generally packaged with nanoparticles or non-nanoparticles for delivery.Despite many advances, the lack of effective and non-toxic delivery methods remains a major factor limiting the application of mRNA.The use of viral systems(such as lentiviruses and adeno-associated viruses)has been widely recognized as an effective way to deliver nucleic acids, but an unwanted immune response is a fundamental barrier to its development.As a result, more and more non-viral vectors(including lipids, polymers, nano-based carriers or functional carriers)have attracted attention as safe and effective delivery vehicles.This paper reviewed the progress, challenges and future directions of various mRNA delivery materials in recent years. [ABSTRACT FROM AUTHOR]