Background and Objectives: In the past two decades, sustainable biological nanomaterials like nanocellulose, encompassing bacterial nanofibers cellulose and wood cellulose nanofibers, have received considerable attention among researchers for diverse product applications. Key attributes such as biocompatibility, biodegradability, renewability, cost-effectiveness, abundant raw material availability, suitable mechanical properties, and safety stand out as pivotal features of biological nanomaterials. Leveraging these properties has led to the creation of numerous applications within various industries. An intriguing product arising from this realm is nanofilm, which is crafted through hydrogen bond formation between cellulose nanofibers, obviating the need for additives or adhesives during production. When utilized as a reinforcement in layered nanocomposite construction, bacterial cellulose nanofibrils (BCNF) and wood cellulose nanofibers (WCNF) exhibit exceptional reinforcement capabilities owing to the inherent cellulose network structure. The recent surge in nanocomposite production emphasis, property optimization, and utilization of high-tech products underscores the growing significance of nanofibrillated cellulose. Recognizing the technical and practical significance of cellulose-layered wood nanocomposite, cellulose nanofilm layers, and the nanocomposites derived from them, coupled with the imperative to thoroughly investigate and evaluate these materials for prospective applied research, this study aims to assess and compare the physical and mechanical properties of cellulose layered wood nanocomposites and bacterial nanocomposites. Materials and Methods: In this study, wood cellulose nanofiber gel and bacterial cellulose nanofiber film were employed. To create wood cellulose nanofiber layered nanocomposites, an initial wood cellulose nanolayer was fabricated using wood cellulose nanofibers and a vacuum-filtration method. Subsequently, the nanolayers underwent solvent displacement with ethanol and acetone through multiple cycles. A mixture of epoxy resin, hardener, and acetone in a 20:10:70 ratio was prepared and agitated on a magnetic stirrer to eliminate bubbles. The cellulose nanolayer was then coated with this epoxy resin, stacking four layers atop one another to form a layered nanocomposite, which was then subjected to a heat press machine and dried for 3 hours at 60 °C. The manufacturing process for bacterial cellulose layered nanocomposites mirrored that of wood cellulose layered nanocomposites, with the distinction that the bacterial cellulose nanofiber film was dried in a hot press at 70 °C for 3 hours to create the bacterial cellulose nanolayer, followed by the assembly of the respective layered nanocomposite. The properties of the resulting nanocomposites were evaluated using various tests, including scanning electron microscopy (FESEM), X-ray diffraction (XRD), infrared spectroscopy (ATR-FTIR), static tension, and water absorption assessments. Results: The study revealed that the average diameter of bacterial nanocellulose and wood nanocellulose measured 45±15 nm and 38±16 nm, respectively. The water absorption of the layered nanocomposite was lower than that of the nanolayer in both nanostructures. Notably, the XRD peaks of bacterial nanocellulose and wood nanocellulose exhibited distinct patterns, with crystallinity levels calculated at 87% and 69%, respectively. Furthermore, the elastic strength, Young's modulus, and strain of the layered nanocomposite composed of bacterial nanocellulose surpassed those of the equivalent composite made from wood nanocellulose. Conclusion: The layered nanocomposites crafted from cellulose nanofibers showcased commendable physical and mechanical attributes, including minimal water absorption and heightened durability. Given these promising characteristics and the escalating interest in related research, it is anticipated that these materials will soon find expanded applications in diverse sectors such as aerospace, automotive, medical and biomedical engineering, food production, film industry, electronics, and magnetism, among others. [ABSTRACT FROM AUTHOR]