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Cilj ovog rada bio je pronaći cpDNA RFLP biljege podesne za usporedbu vrsta roda Hypericum. Vrste H. perforatum, H. maculatum i H. hirsutum uspoređene su na temelju restrikcijskih profila triju međugenskih regija smještenih na velikoj jednostrukoj kopiji cpDNA. Uspoređene su regije između gena psbC - trnS (CS), trnC - trnD (CD), psaA - trnS (AS), a cijepane su sa tri restrikcijske endonukleaze: Alu I, Hae III i Rsa I. Sve tri regije kloroplastnog genoma uspješno su amplificirane lančanom reakcijom polimerazom, dok je restrikcija davala različite rezultate ovisno o kombinaciji fragment - enzim.Najpodesniji cpDNA RFLP biljezi dobiveni su cijepanjem CD enzimom Alu I jer taj restrikcijski profil pokazuje najviši stupanj polimorfizma. Podesne su i regija CS cijepana enzimima Alu I i Hae III, kao i regija AS cijepana enzimom Alu I.

The restriction fragment length polymorphism (RFLP) analysis of three non-coding regions within a large single copy of chloroplast genome are used to examine the phylogenetic relationships among 9 species of genus Hypericum. The PCR-amplified products of regions psaA-trnS, trnC-trnD and psbC-trnS were digested separately with five restriction endonucleases. Gels were scored in a binary format. Wagner parsimony analysis has been performed on the obtained data matrix. A high level of the interspecific variation was detected while intraspecific was not observed. The obtained results indicate discrimination of H. calycinum L. and H. androsaemum L. as the most primitive species in this sample as well as grouping of two related species H. perforatum L. and H. tetrapterum Fries. Also, relation between H. olympicum L. and H. adenotrichum Spach. is closer in terms of the cpDNA analysis than on morphological grounds, while H. richeri subsp. grisebachii (Boiss.) Nyman. is more distant. H. montanum L. is more related to advanced species while relationships of H. hirsutum L. with the other taxa was not resolved completely. The obtained results identifies three intragenic chloroplast DNA regions useful for phylogenetic or phylogeographical analysis in Hypericum.