PCR-RFLP analiza cpDNA roda Hypericum (Clusiaceae)

The restriction fragment length polymorphism (RFLP) analysis of three non-coding regions within a large single copy of chloroplast genome are used to examine the phylogenetic relationships among 9 species of genus Hypericum. The PCR-amplified products of regions psaA-trnS, trnC-trnD and psbC-trnS were digested separately with five restriction endonucleases. Gels were scored in a binary format. Wagner parsimony analysis has been performed on the obtained data matrix. A high level of the interspecific variation was detected while intraspecific was not observed. The obtained results indicate discrimination of H. calycinum L. and H. androsaemum L. as the most primitive species in this sample as well as grouping of two related species H. perforatum L. and H. tetrapterum Fries. Also, relation between H. olympicum L. and H. adenotrichum Spach. is closer in terms of the cpDNA analysis than on morphological grounds, while H. richeri subsp. grisebachii (Boiss.) Nyman. is more distant. H. montanum L. is more related to advanced species while relationships of H. hirsutum L. with the other taxa was not resolved completely. The obtained results identifies three intragenic chloroplast DNA regions useful for phylogenetic or phylogeographical analysis in Hypericum.