301. [The effects of cholecystokinin octapeptide on PKC activity in rat cerebral cortex neurocytes].
- Author
-
Xiang P, Qiu M, Du Z, Chen M, and Wu Z
- Subjects
- Animals, Benzodiazepinones pharmacology, Cerebral Cortex cytology, Devazepide pharmacology, Dose-Response Relationship, Drug, Female, Male, Neurons cytology, Neurons enzymology, Phenylurea Compounds pharmacology, Rats, Rats, Wistar, Receptors, Cholecystokinin antagonists & inhibitors, Cerebral Cortex enzymology, Protein Kinase C metabolism, Sincalide pharmacology
- Abstract
Objective: To investigate the effects of CCK8 on protein kinase C activity in rat cerebral cortex., Methods: The cerebral cortex neurocytes were isolated and used as a model. The effects of CCK8, L-364, 718 and L-365, 260 on PKC activities were detected by using a non-radioactive method., Results: CCK8 caused a detectable increase in PKC activity at 10(-11) mol/L, and a peak increase of PKC activity was observed at 10(-5) mol/L (about 4.5 U/mg protein). PKC activity was increased in dose-dependent manner by CCK8(10(-11)-10(-6) mol/L). The CCKB-selective receptor antagonist L-365, 260 with a higher efficiency, and the CCKA-selective receptor antagonist L-364, 718 with a lower efficiency were able to block a maximal effect of CCK8-induced PKC activation., Conclusions: CCK8 may regulate PKC activities in rat cerebral cortex through CCKB receptor.
- Published
- 2002