Back to Search
Start Over
[The effects of cholecystokinin octapeptide on PKC activity in rat cerebral cortex neurocytes].
- Source :
-
Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao [Hua Xi Yi Ke Da Xue Xue Bao] 2002 Jan; Vol. 33 (1), pp. 72-4, 120. - Publication Year :
- 2002
-
Abstract
- Objective: To investigate the effects of CCK8 on protein kinase C activity in rat cerebral cortex.<br />Methods: The cerebral cortex neurocytes were isolated and used as a model. The effects of CCK8, L-364, 718 and L-365, 260 on PKC activities were detected by using a non-radioactive method.<br />Results: CCK8 caused a detectable increase in PKC activity at 10(-11) mol/L, and a peak increase of PKC activity was observed at 10(-5) mol/L (about 4.5 U/mg protein). PKC activity was increased in dose-dependent manner by CCK8(10(-11)-10(-6) mol/L). The CCKB-selective receptor antagonist L-365, 260 with a higher efficiency, and the CCKA-selective receptor antagonist L-364, 718 with a lower efficiency were able to block a maximal effect of CCK8-induced PKC activation.<br />Conclusions: CCK8 may regulate PKC activities in rat cerebral cortex through CCKB receptor.
- Subjects :
- Animals
Benzodiazepinones pharmacology
Cerebral Cortex cytology
Devazepide pharmacology
Dose-Response Relationship, Drug
Female
Male
Neurons cytology
Neurons enzymology
Phenylurea Compounds pharmacology
Rats
Rats, Wistar
Receptors, Cholecystokinin antagonists & inhibitors
Cerebral Cortex enzymology
Protein Kinase C metabolism
Sincalide pharmacology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0257-7712
- Volume :
- 33
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao
- Publication Type :
- Academic Journal
- Accession number :
- 12599433