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Peanut is one of important food resources worldwide, and its seed quality should be preserved during storage. Chitosan has antifungal activity and good film-forming ability, and natamycin has antifungal activity. In this study, peanut seeds were treated with three dipping solutions, i.e. purified water + 100 Aspergillus flavus spores/ml (control), 1% chitosan + 100 A. flavus spores/ml (treatment-1) and 1% chitosan + 0.2% natamycin + 100 A. flavus spores/ml (treatment-2), and stored at room temperature for 12 months. All treatments decreased fungal growth, insect damage and aflatoxin B1 and increased germination power and normal seed per cent compared with the control after 12 months of storage (P < 0.05). Results showed that chitosan-based coatings incorporated with natamycin treatment might be a practical method in the preservation of peanut seeds during storage. [ABSTRACT FROM AUTHOR]

Compared to the well-studied model legumes, where symbiosis is established via root hair entry, the peanut is infected by Bradyrhizobium through the crack entry, which is less common and not fully understood. Crack entry is, however, considered a primitive symbiotic infection pathway, which could be potentially utilized for engineering non-legume species with nitrogen fixation ability. We utilized a fluorescence-labeled Bradyrhizobium strain to help in understanding the crack entry process at the cellular level. A modified plasmid pRJPaph-bjGFP, harboring the codon-optimized GFP gene and tetracycline resistance gene, was created and conjugated into Bradyrhizobium strain Lb8, an isolate from peanut nodules, through tri-parental mating. Microscopic observation and peanut inoculation assays confirmed the successful GFP tagging of Lb8, which is capable of generating root nodules. A marking system for peanut root potential infection sites and an optimized sample preparation protocol for cryostat sectioning was developed. The feasibility of using the GFP-tagged Lb8 for observing crack entry was examined. GFP signal was detected at the nodule primordial stage and the following nodule developmental stages with robust GFP signals observed in infected cells in the mature nodules. Spherical bacteroids in the root tissue were visualized at the nodules' inner cortex under higher magnification, reflecting the trace along the rhizobial infection path. The GFP labeled Lb8 can serve as an essential tool for plant-microbe studies between the cultivated peanut and Bradyrhizobium, which could facilitate further study of the crack entry process during the legume-rhizobia symbiosis. [ABSTRACT FROM AUTHOR]

Copyright of Acta Agronomica Sinica is the property of Crop Science Society of China and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Agronomica Sinica is the property of Crop Science Society of China and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background: Peanut (Arachis hypogaea L.) is one of the valuable oilseed crops grown in drought-prone areas worldwide. Drought severely limits peanut production and productivity significantly. Method and results: In order to decipher the drought tolerance mechanism in peanut under drought stress, RNA sequencing was performed in TAG − 24 (drought tolerant genotype) and JL-24 (drought susceptible genotype). Approximately 51 million raw reads were generated from four different libraries of two genotypes subjected to drought stress exerted by 20% PEG 6000 stress and control conditions, of which ~ 41 million (80.87%) filtered reads were mapped to the Arachis hypogaea L. reference genome. The transcriptome analysis detected 1,629 differentially expressed genes (DEGs), 186 genes encoding transcription factors (TFs) and 30,199 SSR among the identified DEGs. Among the differentially expressed TF encoding genes, the highest number of genes were WRKY followed by bZIP, C2H2, and MYB during drought stress. The comparative analysis between the two genotypes revealed that TAG-24 exhibits activation of certain key genes and transcriptional factors that are involved in essential biological processes. Specifically, TAG-24 showed activation of genes involved in the plant hormone signaling pathway such as PYL9, Auxin response receptor gene, and ABA. Additionally, genes related to water deprivation such as LEA protein and those involved in combating oxidative damage such as Glutathione reductase were also found to be activated in TAG-24. Conclusion: This genome-wide transcription map, therefore, provides a valuable tool for future transcript profiling under drought stress and enriches the genetic resources available for this important oilseed crop. [ABSTRACT FROM AUTHOR]

Copyright of Food & Machinery is the property of Food & Machinery Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Packaging & Food Machinery is the property of Packaging & Food Machinery Magazine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The aim of this study was to generate knowledge and technology to increase the efficacy of biological products to improve peanut production. The percentage incidence of plant pathogens, germination responses and seedling growth parameters of Arachis hypogaea seeds subjected to algal extracts were evaluated in order to generate a fungal biological agent for application on peanut seeds. Results of this study demonstrate that the application of algal extracts have a positive effect favouring germination responses of peanut seeds. The fungal load detected was the lowest when the algal extract combined with 50% recommended dose of a commercial fungicide combination was applied, demonstrating that this combination of algal extract would represent a new sustainable agronomic practice for the environment since it considerably reduces the use of chemical fungicide agents. These results suggest that the components of algal extracts, such as salicylic and jasmonic acids, have chemical properties that could be considered for the specific control of fungi. This biological product is a new source of bioactive compounds with potential applications in agriculture, showing activity for the control of fungal load of seeds, as well as a promoter of germination. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Agro-Environment Science is the property of Journal of Agro-Environment Science Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

It is evident that legume root nodules can accommodate rhizobial and non-rhizobial bacterial endophytes. Our recent nodule microbiome study in peanuts described that small nodules can harbor diverse bacterial endophytes. To understand their functional role, we isolated 87 indigenous endophytes from small nodules of field-grown peanut roots and characterized them at molecular, biochemical, and physiological levels. The amplified 16S rRNA genes and phylogenetic analysis of these isolates revealed a wide variety of microorganisms related to the genera Bacillus, Burkholderia, Enterobacter, Herbaspirillum, Mistsuaria, Pantoea, Pseudomonas, and Rhizobia. It was observed that 37% (100% identity) and 56% (>99% identity) of the isolates matched with the amplified sequence variants (ASVs) from our previous microbiome study. All of these isolates were tested for stress tolerance (high temperature, salinity, acidic pH) and phosphate (P) solubilization along with ammonia (NH3), indole-3-acetic acid (IAA), 1-aminocyclopropane-1-carboxylate deaminase (ACCD), and siderophore production. The majority (78%) of the isolates were found to be halotolerant, thermotolerant, and acidophilic, and a few of them showed a significant positive response to the production of IAA, NH3, siderophore, ACCD, and P-solubilization. To evaluate the plant growth promotion (PGP) activity, plant and nodulation assays were performed in the growth chamber conditions for the selected isolates from both the non-rhizobial and rhizobial groups. However, these isolates appeared to be non-nodulating in the tested conditions. Nonetheless, the isolates 2 (Pantoea), 17 (Burkholderia), 21 (Herbaspirillum), 33o (Pseudomonas), and 77 (Rhizobium sp.) showed significant PGP activity in terms of biomass production. Our findings indicate that these isolates have potential for future biotechnological applications through the development of biologicals for sustainable crop improvement. [ABSTRACT FROM AUTHOR]

Root rot caused by Fusarium oxysporum is a serious root disease that affects the growth and yield of peanut crops worldwide. Identifying resistance genes is of great significance for both conventional and molecular breeding. At present, there are a few identified disease resistance genes, and the defense response mechanism against this pathogen remains unclear. Using transcriptome analysis techniques, 21 differentially expressed genes (DEGs) were identified in plants treated with Bacillus thuringiensis TG5 and carbendazim. Among these DEGs, the TIFY10A protein gene, pyruvate decarboxylase, and UDP glycosyltransferase 74B1 genes play an important role in early resistance to root rot in peanut plants, leading to regulatory factors that trigger immunity. Our study provides important information on the response mechanism of peanut plants to F. oxysporum-induced root rot and the protective benefits of colonization by the microbial pesticide, B. thuringiensis. [ABSTRACT FROM AUTHOR]

Background: Peanut is an economically-important oilseed crop and needs a large amount of calcium for its normal growth and development. Calcium deficiency usually leads to embryo abortion and subsequent abnormal pod development. Different tolerance to calcium deficiency has been observed between different cultivars, especially between large and small-seed cultivars. Results: In order to figure out different molecular mechanisms in defensive responses between two cultivars, we treated a sensitive (large-seed) and a tolerant (small-seed) cultivar with different calcium levels. The transcriptome analysis identified a total of 58 and 61 differentially expressed genes (DEGs) within small-seed and large-seed peanut groups under different calcium treatments, and these DEGs were entirely covered by gene modules obtained via weighted gene co-expression network analysis (WGCNA). KEGG enrichment analysis showed that the blue-module genes in the large-seed cultivar were mainly enriched in plant-pathogen attack, phenolic metabolism and MAPK signaling pathway, while the green-module genes in the small-seed cultivar were mainly enriched in lipid metabolism including glycerolipid and glycerophospholipid metabolisms. By integrating DEGs with WGCNA, a total of eight hub-DEGs were finally identified, suggesting that the large-seed cultivar concentrated more on plant defensive responses and antioxidant activities under calcium deficiency, while the small-seed cultivar mainly focused on maintaining membrane features to enable normal photosynthesis and signal transduction. Conclusion: The identified hub genes might give a clue for future gene validation and molecular breeding to improve peanut survivability under calcium deficiency. [ABSTRACT FROM AUTHOR]

Heat shock transcription factors (Hsfs) play important roles in plant developmental regulations and various stress responses. In present study, 46 Hsf genes in peanut (AhHsf) were identified and analyzed. The 46 AhHsf genes were classed into three groups (A, B, and C) and 14 subgroups (A1-A9, B1-B4, and C1) together with their Arabidopsis homologs according to phylogenetic analyses, and 46 AhHsf genes unequally located on 17 chromosomes. Gene structure and protein motif analysis revealed that members from the same subgroup possessed similar exon/intron and motif organization, further supporting the results of phylogenetic analyses. Gene duplication events were found in peanut Hsf gene family via syntenic analysis, which were important in Hsf gene family expansion in peanut. The expression of AhHsf genes were detected in different tissues using published data, implying that AhHsf genes may differ in function. In addition, several AhHsf genes (AhHsf5, AhHsf11, AhHsf20, AhHsf24, AhHsf30, AhHsf35) were induced by drought and salt stresses. Furthermore, the stress-induced member AhHsf20 was found to be located in nucleus. Notably, overexpression of AhHsf20 was able to enhance salt tolerance. These results from this study may provide valuable information for further functional analysis of peanut Hsf genes. [ABSTRACT FROM AUTHOR]

Legumes such as peanut (Arachis hypogea) can fulfill most of their nitrogen requirement by symbiotic association with nitrogen-fixing bacteria, rhizobia. Nutrient availability is largely determined by microbial diversity and activity in the rhizosphere that influences plant health, nutrition, and crop yield, as well as soil quality and soil fertility. However, our understanding of the complex effects of microbial diversity and rhizobia inoculation on crop yields of different peanut cultivars under organic versus conventional farming systems is extremely limited. In this research, we studied the impacts of conventional vs. organic cultivation practices and inoculation with commercial vs. single strain inoculum on peanut yield and soil microbial diversity of five peanut cultivars. The experiment was set up in the field following a split-split-plot design. Our results from the 16 S microbiome sequencing showed considerable variations of microbial composition between the cultivation types and inoculum, indicating a preferential association of microbes to peanut roots with various inoculum and cropping system. Alpha diversity indices (chao1, Shannon diversity, and Simpson index) of soil microbiome were generally higher in plots with organic than conventional inorganic practices. The cultivation type and inoculum explained significant differences among bacterial communities. Taxonomic classification revealed two phyla, TM6 and Firmicutes were significantly represented in inorganic as compared to organic soil, where significant phyla were Armatimonadetes, Gemmatimonadetes, Nitrospirae, Proteobacteria, Verrucomicrobia, and WS3. Yields in the organic cultivation system decreased by 10–93% of the yields in the inorganic cultivation system. Cultivar G06 and T511 consistently showed relative high yields in both organic and inorganic trials. Our results show significant two-way interactions between cultivation type and genotype for most of the trait data collected. Therefore, it is critical for farmers to choose varieties based on their cultivation practices. Our results showed that bacterial structure was more uniform in organic fields and microbial diversity in legumes was reduced in inorganic fields. This research provided guides for farmers and scientists to improve peanut yield while promoting microbial diversity and increasing sustainability. [ABSTRACT FROM AUTHOR]

The suspension velocity is the core of the cleaning and sorting mechanisms that utilize a combination of a fan and vibrating sieve. To investigate this, various experimental subjects, such as peanuts with different kernels and clay-heavy clods in different states, were used. The experiment involved simulating the suspension velocity of materials through numerical calculations using fluid dynamics and particle discrete element coupling. The Eularian model was employed to study the coupled gas-solid two-phase flow. The experiment measured the suspension velocities of single and double kernel peanuts, which were found to be 8.34~9.40 m/s and 8.13~9.51 m/s, respectively. Under 20.4% water content and lumpy conditions, the suspension velocities of smaller clods, side by side clods, and larger clods were 12.61~14.30 m/s, 14.16~15.76 m/s and 16.44~18.72 m/s, respectively; under 20.4% water content and smaller clods, the suspension velocities of lumpy and strip of clods were 12.61~14.30 m/s, 11.90~14.13 m/s, respectively; under lumpy and smaller clods, the suspension velocity at 17.6%, 20.4%, and 23.9% water content ranged from 12.38 to 14.20 m/s, 12.61 to 14.30 m/s, and 12.62 to 14.49 m/s, respectively. The simulations showed that the suspension velocity for different types of peanuts, clod sizes, shapes, and water contents was less different from the actual experiments. Specifically, the relative errors in suspension velocity for single-kernel peanuts, double-kernel peanuts, smaller clods, side-by-side clods, larger clods, lumpy clods, strips of clods, and clods with 17.3%, 20.4%, and 23.9% water content were 1.2%, 4.1%, 0.4%, 2.0%, 4.4%, 0.4%, 5.1%, 5.4%, 0.4%, and 1.9%, respectively, compared to actual experiment measurements. The results indicate a significant difference in the suspension velocity between peanuts and clay-heavy clods, which can be distinguished from each other based on this difference. Furthermore, the simulation results have been found to be consistent with the experimental results, thus verifying the feasibility of measuring the material suspension velocity using CFD-DEM gas-solid coupling. [ABSTRACT FROM AUTHOR]

Peanut seeds are susceptible to Aspergillus flavus infection, which has a severe impact on the peanut industry and human health. However, the molecular mechanism underlying this defense remains poorly understood. The aim of this study was to analyze the changes in differentially expressed genes (DEGs) and differential metabolites during A. flavus infection between Zhonghua 6 and Yuanza 9102 by transcriptomic and metabolomic analysis. A total of 5768 DEGs were detected in the transcriptomic study. Further functional analysis showed that some DEGs were significantly enriched in pectinase catabolism, hydrogen peroxide decomposition and cell wall tissues of resistant varieties at the early stage of infection, while these genes were differentially enriched in the middle and late stages of infection in the nonresponsive variety Yuanza 9102. Some DEGs, such as those encoding transcription factors, disease course-related proteins, peroxidase (POD), chitinase and phenylalanine ammonialyase (PAL), were highly expressed in the infection stage. Metabolomic analysis yielded 349 differential metabolites. Resveratrol, cinnamic acid, coumaric acid, ferulic acid in phenylalanine metabolism and 13S-HPODE in the linolenic acid metabolism pathway play major and active roles in peanut resistance to A. flavus. Combined analysis of the differential metabolites and DEGs showed that they were mainly enriched in phenylpropane metabolism and the linolenic acid metabolism pathway. Transcriptomic and metabolomic analyses further confirmed that peanuts infected with A. flavus activates various defense mechanisms, and the response to A. flavus is more rapid in resistant materials. These results can be used to further elucidate the molecular mechanism of peanut resistance to A. flavus infection and provide directions for early detection of infection and for breeding peanut varieties resistant to aflatoxin contamination. [ABSTRACT FROM AUTHOR]

【Objective】 In order to establish the reasonable planting density under the double-row planting model of large ridges and build a high-yielding peanut group model, the impact of the double-row planting density of large ridges (90 cm) on the characteristics, group structure changes and yield of peanut plants was studied.【Method】 The semi-trailing type peanut variety ‘Silihong’ and the erect type ‘Jihua26’were used as experimental materials. Set 6 planting densities: M1 (31.71×104 plants/hm² ), M2 (27.75×104 plants/hm² ), M3 (24.67×104 plants/hm² ), M4 (22.20×104 plants/hm² ), M5 (18.50×104 plants/hm² ), M6 (15.85×104 plants/hm² ), based on single ridge (60 cm) double-seed sowing (11.93×104 holes/hm² ) as a comparison (CK). Determine the seedling rate, plant growth, SPAD value, dry matter accumulation and yield of each peanut variety under different density, and establish a reasonable group structure of peanuts and the optimal planting density of different varieties.【Result】 The emergence time of different varieties was different under different planting densities, the emergence time of ‘Silihong’ was earlier than ‘Jihua 26’. 25 days after sowing, the seedling rate of ‘Silihong’ was up to 100% at M3 density, 30 days after sowing, the seedling rate of ‘Jihua26’ was up to 100% at M3 density, which were significantly higher than other densities. With the decrease of planting density, the height of the main stem and the length of the side branch gradually increase with the decrease of the number of plants per unit area; the SPAD value tends to increase first and then decrease with the decrease of planting density; ‘Jihua 26’ with the decrease of density, the quality of dry matter showed a tendency to rise first and then decrease, and ‘Silihong’ the accumulated amount showed a trend of gradually increasing. With the reduction of planting density, the number of pods per plant, pod yield per plant and weight of 100-pod increased first and then decreased of the two experimental varieties, the highest value was under M4 density of ‘Jihua26’ and M5 density of ‘Silihong’, which were significantly higher than other densities. The yield of the two experimental varieties continues to increase, Jihua 26 at M4 density had highest yield was 7 956.67 kg/hm² and ‘Silihong’ at M5 density had highest yield was 4 790.73 kg/hm², compare to control increase 96.14% and 65.42% respectively. 【Conclusion】 Compared with the singlerow double-seed planting mode, the large ridge double-row + single-seed planting mode can increase the peanut height, SPAD and dry matter accumulation. At the same time, the yield is improved. Under the condition that the density remains unchanged, peanuts can be changed from double-seed to single-seed precision sowing, which increases the yield. When planting in two rows of large ridges, the appropriate sowing density should be selected according to the type of peanut varieties. The M5 density of the semi-trailing type peanut varieties is the best, and the M4 density of the erect type peanut is the best [ABSTRACT FROM AUTHOR]

In tropical and subtropical areas, peanuts are a very important legume crop that is widely cultivated for food and cooking oil. They are, however, extremely susceptible to a wide range of phytopathogens, particularly soil-borne fungi, which result in low yields and poor seed quality. This study aimed to express three Trichoderma asperellum SH16 42 kDa chitinase-encoding genes in peanut to improve their resistance to some soil-borne fungi. Chi42 is a synthetic, intronless, wild-type gene, whereas syncodChi42-1 and syncodChi42-2 are peanut codon-optimized synthetic genes. These genes were introduced into a local peanut strain through Agrobacterium-mediated transformation. Analysis of the transgenic peanut lines showed that chitinase-specific activities from the syncodChi42-1 and syncodChi42-2 genes were approximately 1.2 and 1.4 times higher than those of the wild-type gene, respectively. The engineered peanut plants also exhibited antifungal activity against the soil-borne pathogenic fungus Sclerotium rolfsii. The transgenic peanut lines transformed with the two synthetic genes have stronger antifungal activities than those transformed with the wild-type version, suggesting that they could be used as novel peanut lines to combat phytopathogenic fungi. [ABSTRACT FROM AUTHOR]

To clarify the improvement effect of organic fertilizer application on peanut continuous cropping obstacles, a field plot experiment was conducted in a plot that had been continuously cropped with peanut for 5 years. Equal amounts of nitrogen were applied to the plot using charcoal-based fertilizer (T2), pig manure compost (T3), and chicken manure compost (T4), while chemical fertilizer (T1) and no fertilizer (CK) were used as controls. The study investigated peanut agronomic traits, yield and its contributing factors, soil physical and chemical properties, and peanut leaf diseases. The results showed that all three types of organic fertilizers could significantly promote peanut growth, with the T3 performing the best. The main stem height, length of the first lateral branch, and number of branches per plant in the T3 were 31.45%, 21.28%, and 26.32% higher than those in the CK, respectively. There was no significant difference between the T2 and T4 s in terms of main stem height and number of branches per plant compared to the T3. In terms of yield and its contributing factors, the performance of organic fertilizers was significantly better than that of the T1 and CK s. Among them, the T3 had the highest yield, which was 36.81% and 22.07% higher than that of the CK and T1 s, respectively. There was no significant difference between the T4 and the T3. The T2 and T4 s had the highest hundred-pod weight, while the T3 and T4 s had the lowest number of pods per kilogram. There was no significant difference in hundredkernel weight among the three types of organic fertilizers. In terms of soil physical and chemical properties, the application of organic fertilizers significantly increased soil pH, porosity, field water holding capacity, and decreased soil bulk density compared to chemical fertilizer and no-fertilizer s. There was no significant difference in these indicators among the organic fertilizer s. In terms of soil nutrient content, all fertilization s significantly increased the content of total nitrogen, alkaline nitrogen, available phosphorus, and available potassium in the soil. Among them, the organic fertilizer treatments were significantly better than the chemical fertilizer in terms of total nitrogen and available phosphorus content. Additionally, organic fertilizer s significantly increased the soil organic carbon content, with the T2 having the highest organic carbon content, which was 20.56% higher than that of the CK. In terms of soil phenolic acid content, organic fertilizer s significantly reduced the content of vanillic acid and coumarin in the soil, with the lowest coumarin content observed in the T3 and T4 s. In terms of peanut leaf diseases, the disease index of peanut leaf spot and brown spot were significantly lower in the organic fertilizer s than in the no-fertilizer and chemical fertilizer s, but there was no significant difference between the different organic fertilizer s. After the comprehensive evaluation of the affiliation function method, it can be found that T4 can alleviate the continuous cropping obstacle to the greatest extent. The conclusion drawn from this study is that biochar-based fertilizer, pig manure compost, and chicken manure compost can all promote growth, increase yield, improve soil environmental quality, and provide certain disease prevention and control effects for peanut plants under continuous cropping obstacles. It is recommended to choose organic fertilizer for application in areas where peanut continuous cropping is more serious in a timely and site-specific manner. [ABSTRACT FROM AUTHOR]

Peanut (Arachis hypogaea) is an important oilseed and cash crop worldwide, contributing an important source of edible oil and protein for human nutrition. However, the incidence of stem rot disease caused by Athelia rolfsii poses a major challenge to peanut cultivation, resulting in significant yield losses. In this study, a panel of 202 peanut accessions was evaluated for their resistance to stem rot by inoculating plants in the field with A. rolfsii-infested oat grains in three environments. The mean disease index value of each environment for accessions in subsp. fasitigiate and subsp. hypogaea showed no significant difference. Accessions from southern China displayed the lowest disease index value compared to those from other ecological regions. We used whole-genome resequencing to analyze the genotypes of the accessions and to identify significant SNPs associated with stem rot resistance through genome-wide association study (GWAS). A total of 121 significant SNPs associated with stem rot resistance in peanut were identified, with phenotypic variation explained (PVE) ranging from 12.23% to 15.51%. A total of 27 candidate genes within 100 kb upstream and downstream of 23 significant SNPs were annotated, which have functions related to recognition, signal transduction, and defense response. These significant SNPs and candidate genes provide valuable information for further validation and molecular breeding to improve stem rot resistance in peanut. [ABSTRACT FROM AUTHOR]

Food challenges (FCs) form the basis for assessing efficacy outcomes in interventional studies of food allergy; however, different studies have used a variety of similar but not identical criteria to define a challenge reaction, including subjective (nonobjective) symptoms occurring in a single-organ system as dose limiting. Our aim was to undertake a secondary analysis of 4 interventional studies to assess the impact of using less objective criteria to determine challenge-stop on reaction thresholds and their reproducibility. We analyzed individual participant data, including individual participant data meta-analysis, by using 3 different published challenge-stop criteria: (1) PRACTALL consesus criteria; (2) Consortium for Food Allergy Research version 3 (CoFAR v3) with at least 1 moderate- or severe-grade symptom; or (3) CoFAR v3 with at least 2 mild symptoms occurring in different organ systems. Reproducibility of challenge threshold was also assessed in participants undergoing subsequent repeat FCs. Four studies, with detailed challenge data from a total of 592 participants, were included. Applying CoFAR v3 definitions for dose-limiting symptoms resulted in an underestimate of reaction thresholds compared with those in PRACTALL (P <.001) that is equivalent to almost a single dosing increment when using a semi-log dosing regimen. Reproducibility was also reduced when applying CoFAR v3 (P <.001 [n = 223]). Using the least conservative interpretation of CoFAR v3 (≥2 mild symptoms occurring in different systems) resulted in a significant overestimate of 15% when assessing oral immunotherapy efficacy. Applying a data-driven minor modification to CoFAR v3 resulted in a new set of challenge-stop criteria with validity similar to that of PRACTALL but one that is simpler to implement and in which significant gastrointestinal discomfort with observable decreased activity remains a dose-limiting symptom. The use of less objective symptoms to define challenge-stop compromises the reproducibility of the FC as a tool to assess efficacy outcomes in interventional studies, and potentially overestimates the efficacy of the intervention tested. [Display omitted] [ABSTRACT FROM AUTHOR]

The article discusses research which described a modified prototype for peanut decorticator fabricated using Autocad software at the laboratory of Sam Higginbottom Institute of Agriculture science and technology. The study discusses the principle in designing the pedal operated peanut decorticator. It details the experimental setup and assembly of the model including the constructional parts consisting of the main frame, hopper, feeder cup, concave, rocker arm, rasp-bar, shaft and bushing.

Copyright of Chinese Journal of Oil Crop Sciences is the property of Oil Crops Research Institute of Chinese Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Oil Crop Sciences is the property of Oil Crops Research Institute of Chinese Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Oil Crop Sciences is the property of Oil Crops Research Institute of Chinese Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Oil Crop Sciences is the property of Oil Crops Research Institute of Chinese Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Gyermekgyógyászat is the property of Semmelweis Kiado and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Cu, Zn, Mn, and B) element contents of the Halisbey peanut variety. The nutrient requirements, specific nutrient management strategies, and adaptation of the variety to the regions where it was grown were also assessed. According to the average results of the N applications, different doses affected the levels of N (240N+B: 1.76%) alongside the content of other macro-elements, as well as sodium (200N: 0.09 mg kg-1), iron (80N: 32.39 mg kg-1), and copper (40N: 14.11 mg kg-1) among the micro-elements. The bacterial application was not found to significantly increase N content (240N+B: 1.76%), calcium content (0.08%) and zinc content (49.68 mg kg-1). At the same time, (240N+B) bacteria and N application increased the N ratio. [ABSTRACT FROM AUTHOR]

Rhizobia form symbiotic relationships with legumes, fixing atmospheric nitrogen into a plant-accessible form within their root nodules. Nitrogen fixation is vital for sustainable soil improvements in agriculture. Peanut (Arachis hypogaea) is a leguminous crop whose nodulation mechanism requires further elucidation. In this study, comprehensive transcriptomic and metabolomic analyses were conducted to assess the differences between a non-nodulating peanut variety and a nodulating peanut variety. Total RNA was extracted from peanut roots, then first-strand and second-strand cDNA were synthesized and purified. After sequencing adaptors were added to the fragments, the cDNA libraries were sequenced. Our transcriptomic analysis identified 3362 differentially expressed genes (DEGs) between the two varieties. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses revealed that the DEGs were mainly involved in metabolic pathways, hormone signal transduction, secondary metabolic biosynthesis, phenylpropanoid biosynthesis, or ABC transport. Further analyses indicated that the biosynthesis of flavonoids, such as isoflavones, flavonols, and flavonoids, was important for peanut nodulation. A lack of flavonoid transport into the rhizosphere (soil) could prevent rhizobial chemotaxis and the activation of their nodulation genes. The downregulation of AUXIN-RESPONSE FACTOR (ARF) genes and lower auxin content could reduce rhizobia's invasion of peanut roots, ultimately reducing nodule formation. Auxin is the major hormone that influences the cell-cycle initiation and progression required for nodule initiation and accumulates during different stages of nodule development. These findings lay the foundation for subsequent research into the nitrogen-fixation efficiency of peanut nodules. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Groundnut productivity and quality have been impeded by rising temperatures in semi-arid environments. Hence, understanding the effects and molecular mechanisms of heat stress tolerance will aid in tackling yield losses. In this context, a recombinant inbred line (RIL) population was developed and phenotyped for eight seasons at three locations for agronomic, phenological, and physiological traits under heat stress. A genetic map was constructed using genotyping-by-sequencing with 478 single-nucleotide polymorphism (SNP) loci spanning a map distance of 1,961.39 cM. Quantitative trait locus (QTL) analysis using phenotypic and genotypic data identified 45 major main-effect QTLs for 21 traits. Intriguingly, three QTL clusters (Cluster-1-Ah03, Cluster-2-Ah12, and Cluster-3-Ah20) harbor more than half of the major QTLs (30/45, 66.6%) for various heat tolerant traits, explaining 10.4%-38.6%, 10.6%-44.6%, and 10.1%-49.5% of phenotypic variance, respectively. Furthermore, important candidate genes encoding DHHC-type zinc finger family protein (arahy.J0Y6Y5), peptide transporter 1 (arahy.8ZMT0C), pentatricopeptide repeat-containing protein (arahy.4A4JE9), Ulp1 protease family (arahy.X568GS), Kelch repeat F-box protein (arahy.I7X4PC), FRIGIDA-like protein (arahy.0C3V8Z), and post-illumination chlorophyll fluorescence increase (arahy.92ZGJC) were the underlying three QTL clusters. The putative functions of these genes suggested their involvement in seed development, regulating plant architecture, yield, genesis and growth of plants, flowering time regulation, and photosynthesis. Our results could provide a platform for further fine mapping, gene discovery, and developing markers for genomics-assisted breeding to develop heat-tolerant groundnut varieties. [ABSTRACT FROM AUTHOR]

Background and Objectives: Rainfed planting of peanuts in Guilan province has a history of more than 120 years. The use of late cultivar NC2 in most of the peanut fields in this region during the last 45 years has provided the basis for the spread of diseases and severe functional fluctuations in the production of this plant. The light soil texture in the fields of this plant also increases the yield fluctuations of this cultivar in the low rainy years by reducing the available moisture for peanut plants. As a result, the introduction of new cultivars adapted to the climatic conditions of the region, which have good pod yield in rainfed conditions, is one of the most important necessity for Guilan province. Therefore, the present study was designed and conducted to investigate and compare physiological indices and radiation use efficiency in 14 new peanut genotypes and compare them with NC2 cultivar. Materials and Methods: This study was conducted as a randomized complete block design with three replications in two years 2016 and 2017 in Astaneh Ashrafiyeh city. 14 peanut genotypes along with NC2 cultivar, which is the dominant cultivar in the region, formed experimental blocks. All genotypes were planted flat and in rainfed conditions. Heat unit throughout the growth period, crop growth rate, pod growth rate, partitioning coefficient, radiation use efficiency, pod yield, grain yield, biological yield and harvest index were studied in all genotypes and NC2 cultivar and were compared with this cultivar. Results: The results of this study indicated that the highest heat units from planting to harvesting maturity were observed in NC2 cultivar and ICGV02317 and ICGV92222 genotypes, respectively, while ICGV00350 and ICGV87846 genotypes had the lowest heat units during the growing season. The highest values of crop growth rate, pod growth rate, radiation use efficiency, pod yield and grain yield were obtained from NC2 cultivar and ICGV00420, ICGV99019 and ICGV92116 genotypes. Conclusion: According to the results, in rainfed conditions, 3 genotypes ICGV00420, ICGV99019 and ICGV92116 reached the harvesting maturity stage faster than NC2 cultivar with a lower heat unit in this area while in many of the measured traits were similar to the mentioned cultivar. The results also indicated that the mentioned genotypes can be considered as an alternative to planting NC2 cultivar in the region's farms according to the calculated physiological indices and radiation use efficiency because these genotypes had suitable radiation use efficiency, pod and grain yield under rainfed cultivation conditions. [ABSTRACT FROM AUTHOR]

Seed treatment against soil pests and soil-borne pathogenic fungi, foliar application of microelement and mepiquat chloride at flowering and after flowering periods promise to increase peanut (Arachis hypogaea L.) yield. A two-year field study was conducted to test this hypothesis by seed fungicide and insecticide treatment, foliar micronutrient and mepiquat chloride (Pix) applications at different combinations and different growth stages of peanut in the eastern Mediterranean region. The treatments were different combinations of Vitavax (200 g/L Carboxin + 200 g/L Thiram) 400 g/L 100 kg seed, Gaucho (600 g/L Imidacloprid) 800 g/L 100 kg seed, Fertilon Combi (Fe, Zn, B, Mn, Cu, Mn and Mo) 1000 g /ha and Pix (150 cc /ha mepiquat chloride). Peanut seeds were treated with fungicide and insecticide before planting. Microelement fertilizer and Pix combinations were applied at the beginning of flowering, after flowering and at the beginning of flowering + after flowering. The best results for seed kernel ratio, 100 seed weight and pod yield were obtained from Pix + Fertilon Combin treatment after flowering. This treatment was followed by Goucho + Vitavax seed treatment before planting + Pix application after flowering. In this study, it was observed that Goucho + Vitavax seed treatment and Pix, Fertilon Combi and Pix + Fertilon Combi applications at flowering, after flowering and at flowering + after flowering applications generally contributed pod yield increases. [ABSTRACT FROM AUTHOR]

Summary: Trans‐resveratrol is a natural polyphenolic compound, belonging to the class of stilbenes. It is known for its various pharmacological properties but limited bioavailability. Our objective was to evaluate for the first time the effect of an extract of Sargassum vulgare on resveratrol, piceatannol and piceid production in peanut (Arachis hypogaea) sprouts. Treatments with rising concentrations of seaweed extract were assessed in 'Trabelsia', 'Chanfakhi' and 'American' peanut varieties after 7 days of incubation. Liquid chromatography‐mass spectrometry was performed to quantify targeted compounds. Total phenols and flavonoids content, PAL and TAL activity and DPPH radical scavenging activity were also determined. A significant genotype effect was demonstrated, as quite exceptionally, in 'Trabelsia' the resveratrol content was increased by a factor 110 by elicitation with 8% extract. Piceatannol, piceid, TPC, TFC, PAL, TAL, and DPPH radical scavenging activity confirmed the same trend. This study provides a new insight into an effective approach to enable the accumulation of resveratrol and its derivatives in peanut sprouts for use as a functional food. [ABSTRACT FROM AUTHOR]

In order to further understand types of peanut diseases in field, the regularity of main diseases and influencing factors in northern Henan province, accurately prevent and control the field diseases during peanut growth period, in this study, peanut diseases in field were investigated in the field plots including the spring sowing continuous cropping(winter fallow) and peanut wheat rotation fields in Nanzhaoshi village, Datun township, Puyangcity from 2019 to 2021. The occurrence regularity of the main diseases was analyzed, and the influencing factors were studied. The investigation found that the diseases at the early stage of peanut growth were mainly stem rot, early leaf spot and scorch spot and so on, the diseases in at the late stage of growth were mainly early leaf spot, late leaf spot, web blotch, southern blight, and so on. Among them, leaf spot disease was a serious disease that occurred every year during the growth of peanut in the tested field. The correlation analysis results showed that the correlations between the disease index of leaf spot and the average relative humidity of ten days as well as average value of average temperature of ten days were 0.373, -0.311, respectively, reached the extremely significant and significant levels, respectively, indicating that the temperature and the relative humidity had significant and extremely significant impacts on the occurrence of leaf spot. From the results of general linear models univariate analysis, the effects of tillage methods, years, and their interaction on the occurrence of leaf spot disease were not significant, the continuous cropping effect and accumulation of pathogenic bacteria in different years were not obvious. With the advance of growth period, the incidence of leaf spot disease increased, which may be related to the peanut leaf spot disease mainly caused by late leaf spot disease in northern China, as well as the accumulation and repeated infection of leaf spot pathogens in the fields in the year. This study clarified the types, occurrence regularity, and influencing factors of peanut field diseases in northern Henan, and provided a theoretical basis for targeted disease prevention and control. [ABSTRACT FROM AUTHOR]

The objective of this study was to evaluate the impact of phosphorus deficiency in the interaction between the biofertilizer Serratia sp. S119 strain and peanut and maize plants. Plant assays were performed under P deficiency and several parameters of plant-bacterial interaction were measured. Bacterial chemotaxis, root colonization and biofilm production were measured as the first steps of the plant-bacteria interaction. Also, the effect of root exudates of plants grown in P deficiency on the ability to solubilize insoluble phosphate of Serratia sp. S119 was analyzed. Results were further correlated with analyzes of the pqqE gene expression and the measurement of the activity of pqq promoter. Finally, the effect of the root exudates on the ACC deaminase activity of S119 strain was measured. Results indicated that plant root exudates obtained under P-deficient conditions positively attract Serratia sp. S119 and stimulate its colonization and biofilm formation. These plant root compounds showed also to produce a stimulating effect of the phosphate solubilization ability and ACC deaminase activity of S119 strain. Finally, root exudates obtained under P-deficient conditions induce pqqE gene expression and the activity of pqq promoter of S119 strain. In conclusion, root exudates contain specific signal molecules that attract phosphate solubilizing bacteria to the rhizosphere of peanut and maize plants growing in P deficient condition. Signal molecules would favor the colonization and establishment of the PSB strain in the surface tissues as well as in inner tissues, stimulate phosphate solubilization ability and enhance ACC deaminase activity to decrease plant's nutritional stress response. [ABSTRACT FROM AUTHOR]

Micronutrient fertilization is usually neglected by producers, especially for peanut, a crop that is frequently grown in crop rotation systems due to its low perceived nutrient requirements. New peanut cultivars are able to achieve high yields when grown under suitable conditions. However, fertilization recommendation tables are dated and do not consider the need for micronutrients. To support improvements in these recommendations, this study quantified the micronutrient demand (B, Cu, Fe, Mn, and Zn) of three runner peanut cultivars (IAC Runner 886, IAC 505, and IAC OL3) during the biological cycle and the transport of these micronutrients to pods and kernels. The experiment was carried out in a randomized complete block with a split-plot design and nine replications. The whole plots consisted of the three peanut cultivars, and the subplots comprised nine plant samplings (at 14, 28, 42, 56, 70, 84, 105, 126, and 147 days after emergence (DAE)). These modern peanut cultivars exhibited high uptake and accumulation of Fe, but the proportion of Fe removed by pods and kernels was lowest among all analyzed micronutrients. The second-most-accumulated micronutrient was Mn. The maximum requirement for micronutrients of peanut occurred around 84 DAE, and IAC 505 had the highest micronutrient uptake and accumulation among the cultivars (especially at later stages), followed by IAC OL3 and IAC Runner 886. Our results provide new insights into micronutrient requirements for peanut and demonstrate the need for new fertilizer recommendation programs for peanut cultivation. [ABSTRACT FROM AUTHOR]

Quantitative Trait Loci (QTL) mapping has been thoroughly used in peanut genetics and breeding in spite of the narrow genetic diversity and the segmental tetraploid nature of the cultivated species. QTL mapping is helpful for identifying the genomic regions that contribute to traits, for estimating the extent of variation and the genetic action (i.e., additive, dominant, or epistatic) underlying this variation, and for pinpointing genetic correlations between traits. The aim of this paper is to review the recently published studies on QTL mapping with a particular emphasis on mapping populations used as well as traits related to kernel quality. We found that several populations have been used for QTL mapping including interspecific populations developed from crosses between synthetic tetraploids and elite varieties. Those populations allowed the broadening of the genetic base of cultivated peanut and helped with the mapping of QTL and identifying beneficial wild alleles for economically important traits. Furthermore, only a few studies reported QTL related to kernel quality. The main quality traits for which QTL have been mapped include oil and protein content as well as fatty acid compositions. QTL for other agronomic traits have also been reported. Among the 1261 QTL reported in this review, and extracted from the most relevant studies on QTL mapping in peanut, 413 (~33%) were related to kernel quality showing the importance of quality in peanut genetics and breeding. Exploiting the QTL information could accelerate breeding to develop highly nutritious superior cultivars in the face of climate change. [ABSTRACT FROM AUTHOR]

Copyright of Food & Machinery is the property of Food & Machinery Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Peanut rust caused by Puccinia arachidis is one of the most important peanut foliar diseases in the world, particularly in Burkina Faso. For sustainable peanut production, ecological disease management strategies are needed to limit the overuse of synthetic fungicides in the control of peanut rust. Thus, the use of Akanthomyces lecanii, a hyperparasite of rust fungi, may be an alternative to control peanut rust. However, investigations of the mechanisms of action of A. lecanii are essential before its development as a biocontrol agent. To do so, peanut leaves bearing rust pustules were detached, and each leaf was sprayed with 500 µl of a suspension of A. lecanii conidia (106 conidia/ml) at the lower surface. Then, each leaf was incubated in a Petri dish on moist blotting paper, and kept in the laboratory at 20 °C, under a 12:12 light/dark photoperiod. After 15 days incubation, the inoculated and uninoculated rust pustules were observed under photonic and electron microscopes Pictures revealed that A. lecanii colonized the urediniospores of inoculated sori. Our results showed that the mechanisms of action of A. lecanii on P. arachidis could involve the following events: (i) attachment of the antagonist to urediniospores mediated by a mucilaginous extracellular matrix; (ii) penetration due to mechanical pressure and enzymes action on the cell-wall; (iii) active growth of the antagonist inside of urediniospores and digestion of the cell content. From our study, the use of suspensions of A. lecanii spores is a promising tool for biocontrol of peanut rust. [ABSTRACT FROM AUTHOR]

Oligo probe staining is a low-cost and efficient chromosome identification technique. In this study, oligo genomic in situ hybridization (Oligo-GISH) technology was established in peanut. Peanut A and B subgenome-specific interspersed repeat (IR) oligo probe sets were developed based on clustering and electronic localization of tandem repeat sequences in the reference genome of Tifrunner. The Oligo-GISH kit was then used to perform staining of 15 Arachis species. The A-subgenome probe set stained the chromosomes of A- and E-genome Arachis species, the B-subgenome probe set stained those of B-, F-, K-, and E-genome species, and neither set stained those of H-genome species. These results indicate the relationships among the genomes of these Arachis species. The Oligo-GISH kit was also used for batch staining of the chromosomes of 389 seedlings from the irradiated M1 generation, allowing 67 translocation and deletion lines to be identified. Subsequent Oligo-FISH karyotyping, FISH using single-copy probe libraries, and trait investigation identified seven homozygous chromosomal variants from the M3 generation and suggested that there may be genes on chromosome 4B controlling seed number per pod. These findings demonstrate that the IR probe sets and method developed in this study can facilitate research on distant hybridization and genetic improvement in peanut.

Plant oils have been applied for numerous purposes. Developing the composition of oils through nanotechnology has become a requirement, whether from a medical or industrial point of view. In this study, nanoemulsions (NEs) of olive and peanut oils were evaluated. GC-MS was used to determine the saturated and unsaturated fatty acids contents in both oils. Based on the area %, cis-8,11,14-eicosatrienoic acid (54.0%), myristic acid (30.7%), and arachidonic acid (23.1%) were the greatest constituents in peanut oil, while arachidonic acid (23.2%), cis-11,14,17-eicosatrienoic acid (22.7%), and cis-11-eicosenoic acid (11.4%) were the greatest constituents in olive oil. TEM examination indicated that the diameter of peanut oil NEs (14.6 nm) was less than that of olive oil NEs (24.5 nm). Olive oil and its NEs exhibited more antioxidant activity than peanut oil and its NEs had IC50 values of 158.6, 102.5, 435.1, and 291.5 µg/mL, respectively. Negligible hemolysis was observed using olive oil, unlike peanut oil, while hemolysis based olive oil NEs was increased compared with hemolysis based peanut oil NEs, particularly at high concentrations of 600 to 1000 µg/mL. Molecular docking investigation offered the structure–activity correlation and binding modes of cis-8,11,14-eicosatrienoic acid with Salmonella typhi (5ZXM) enzymes.

This study was conducted to determine the macro and micro element contents of fourteen peanuts in Turkey. Virginia (NC-7, Halisbey, Arıoğlu-2003, Sultan Flower-22, Osmaniye-2005, Brantley, Wilson, Batem-5025, Batem-Cihangir, NC V 11 and Polen) Runner (Georgia Green) and Spanish (Florispan) market types have been evaluated. The research was conducted for two years (2015 to 2016) under main crop conditions in the trial areas of belonging to the Oil Seed Research Institute. The highest nitrogen content is from the Florispan (4.56%) variety, the highest phosphorus and sodium content is from the Halisbey (0.10%) variety, the highest potassium content is from the Sultan (0.46%) variety, the highest calcium content is from the Flower-22 (0.07%) variety and the highest the magnesium content was taken from Arıoğlu-2003 (0.26%) variety. The highest iron content is in Batem-Cihangir (27.34 mg kg-1) variety, the highest copper content is in Flower-22 (7.08 mg kg-1), the highest zinc content is in Sultan (29.35 mg kg-1), the highest manganese content NC-7 (20.61 mg kg-1) variety, the highest boron content was found in Florispan (26.99 mg kg-1) variety. According to the results of this study, varieties with different chemical compositions can be used in food and breeding studies.

The growth of vision transformer (ViT) methods have been quite enormous since its features provide efficient outcome in image classification, and identification. Inspired of this beneficial, this paper propose an Enhanced vision transformer architecture (EViTA) model for pest identification, segmentation, and classification. The as of late found that, compare to machine learning, Convolutional neural network algorithms the ViT has providing trusted results on image classification. Motivated by this, in this paper, we concentrate on the best way to learn dual barch segment representations in ViT models for image arrangement. Based upon its features, here propose a double layer transformer encoder to integrate pest image segments of various sizes of pest images to create more grounded image highlights. The current study uses, three pest datasets that affects peanut crops such as Aphids (IP102 Dataset), Wireworm (IP102 Dataset), and Gram Caterpillar collected from public available repository. Our methodology processes small segment and huge segment of tokens with two separate parts of various computational intricacy also, these tokens are then combined simply by consideration numerous times to complete one another. The taken datasets’ are preprocessed utilizing the characteristic by using moth flame optimization (MFO), and flatten the images by using linear projector methodology to enhance the missing quality in pest images, and afterward normalization methods are executed to switch it over completely in to mathematical arrangement. This processed information is standardized further utilizing the self attention in StandardScaler procedures are carried out for choosing the ideal highlights in the dataset accordingly having huge effect towards affecting pest image predictions. These ideal highlights are at last taken care of into the EViTA model and the outcomes created are considered in contrast to the cutting edge models which at last legitimize the predominance of the proposed EViTA+PCA+MFO model in pest image prediction with high accuracy rate. Broad trials show that our methodology performs better compared to or on standard with a few simultaneous deals with vision transformer, notwithstanding productive CNN models.

Abstract Background Testa color is an important trait of peanut (Arachis hypogaea L.) which is closely related with the nutritional and commercial value. Pink and red are main color of peanut testa. However, the genetic mechanism of testa color regulation in peanut is not fully understood. To elucidate a clear picture of peanut testa regulatory model, samples of pink cultivar (Y9102), red cultivar (ZH12), and two RNA pools (bulk red and bulk pink) constructed from F4 lines of Y9102 x ZH12 were compared through a bulk RNA-seq approach. Results A total of 2992 differential expressed genes (DEGs) were identified among which 317 and 1334 were up-regulated and 225 and 1116 were down-regulated in the bulk red-vs-bulk pink RNA pools and Y9102-vs-ZH12, respectively. KEGG analysis indicates that these genes were divided into significantly enriched metabolic pathways including phenylpropanoid, flavonoid/anthocyanin, isoflavonoid and lignin biosynthetic pathways. Notably, the expression of the anthocyanin upstream regulatory genes PAL, CHS, and CHI was upregulated in pink and red testa peanuts, indicating that their regulation may occur before to the advent of testa pigmentation. However, the differential expression of down-stream regulatory genes including F3H, DFR, and ANS revealed that deepening of testa color not only depends on their gene expression bias, but also linked with FLS inhibition. In addition, the down-regulation of HCT, IFS, HID, 7-IOMT, and I2’H genes provided an alternative mechanism for promoting anthocyanin accumulation via perturbation of lignin and isoflavone pathways. Furthermore, the co-expression module of MYB, bHLH, and WRKY transcription factors also suggested a fascinating transcriptional activation complex, where MYB-bHLH could utilize WRKY as a co-option during the testa color regulation by augmenting anthocyanin biosynthesis in peanut. Conclusions These findings reveal candidate functional genes and potential strategies for the manipulation of anthocyanin biosynthesis to improve peanut varieties with desirable testa color.

The current study was carried out to assess the antioxidant expression of the seed’s black-testa peanut cultivar namely CNC1 in the artifical drought condition. The oxidative stress with a burst of O2.- and H2O2 in peanut leaves resulted the obviously cellular membrane damage that was illustrated by an increase of lipid peroxidation. A high induced activity of enzymatic antioxidants such as SOD, CAT and POX would detoxify the excess of O2.- and H2O2. An increase in content of amino acid proline protects the stressed cells by adjusting intercellular osmotic potential. The antioxidative expression of CNC1 plants was similar to that in L20 cutivar - a drought tolerant cutivar of peanut.

Migratory locust has been widely reported as a quality protein source. However, there are food safety problems related with the usage of insects as food. In this study, migratory locust powder (MLP) was used as a protein supply alternative to milk powder (MP) in peanut-based RUTFs. Seven formulations were obtained at different substitution levels (0 – 30%) of MP with MLP. Pesticide residues, aflatoxins, triazine and toxic metals were analysed using a GC-MS system and Atomic Absorption Spectrophotometer. Formulation with 30% MLP and without milk have the highest level of organochlorine (3.22 µg/kg), organophosphorus (0.40-4.56µg/kg) amongst others. Aflatoxins of the therapeutic foods increased with increasing levels of MLP. The heavy metals, melamine, and cyanuric acid of the RUTFs were below the standard permissible limits. Migratory locust powder could be used as an alternate protein source in the formulation of peanut based RUTFs without posing health threat.

Abstract Background Polyhalite fertilizer application is an effective way to alleviate a shortage of potassium. This study explored the effects of polyhalite fertilizer application as a total or partial replacement for conventional potassium fertilizer to improve peanut growth and soil quality. Results The index of peanut yield and its economic benefits, the content and distribution of mineral nutrients in different organs, soil chemical properties, and rhizosphere microbial diversity in response to the treatments were examined. The results show that the M4P6T treatment (60% polyhalite fertilizer replacing potassium chloride as the base fertilizer, and 40% potassium chloride fertilizer applied as a topdressing) increased profit by 7.2% without affecting the yield. The M4P6T treatment significantly improved the accumulation and distribution of potassium, calcium and magnesium in the kernels compared with the M10B treatment (no polyhalite fertilizer; potassium chloride fertilizer only as the base fertilizer). Soil treated with polyhalite fertilizer had higher alpha-diversity values and greater relative abundance of microbes at the phylum and genus levels. Conclusions Partial substitution of polyhalite for potassium chloride improved soil quality and peanut growth more than did single applications of polyhalite and potassium chloride. Graphical Abstract

Biochar-based fertilizers, customized to specific soil conditions, have piqued public attention to enhance soil quality and carbon sequestration. The incorporation of biochar into agricultural fields can become a primary factor in the preservation of soil productivity and fertility. However, there is still no conclusive experimental evidence to support this claim. In this study, a split-plot experiment was carried out according to a randomized complete block design (RCBD) using three replications to evaluate the influence of irrigation and varied amounts of biochar-based fertilizer on peanut plants' yield to determine the water use efficiency (WUE). The primary treatment with different irrigation cycles and no irrigation, and the sub-treatment with biochar-based fertilizer of 50, 100, and 150 kg N ha-1 and no fertilizer, were performed in Sulaymaniyah which is located in the east of the Kurdistan Region of Iraq. The results revealed that the seven-day irrigation cycle produces the largest pods and seeds, with 4325 and 2435 kg ha-1 yields, respectively. The 100 kg ha-1 biochar-based fertilizer yielded the highest output, with a pod yield of 3652 kg ha-1 and a seed yield of 1921 kg ha-1. According to the results of the interaction between irrigation management and biochar-based fertilizer, the irrigation treatment had a maximum seed yield of seven-day and the fertilizer treatment had a maximum seed output of 100 kg N ha-1 with an average of 3462 kg ha-1. The variance analysis results indicate that water use efficiency in irrigation management, different levels of biochar-based fertilizer, and their combined effect on total biomass, seed, and pods were significant.