189 results on '"Schaffrath, Raffael"'
Search Results
152. Shiga toxins and their mechanisms of cell entry
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Sandvig, Kirsten, Wälchli, Sébastien, Lauvrak, Silje U., Schmitt, Manfred J., editor, and Schaffrath, Raffael, editor
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- 2005
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153. Cholera toxin: mechanisms of entry into host cells
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Saslowsky, David E., Kothe, Michael, Lencer, Wayne I., Schmitt, Manfred J., editor, and Schaffrath, Raffael, editor
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- 2005
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154. Ricin: structure, synthesis, and mode of action
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Lord, J. Michael, Roberts, Lynne M., Schmitt, Manfred J., editor, and Schaffrath, Raffael, editor
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- 2005
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155. The Ustilago maydis killer toxins
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Bruenn, Jeremy, Schmitt, Manfred J., editor, and Schaffrath, Raffael, editor
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- 2005
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156. S. cerevisiae K28 toxin – a secreted virus toxin of the A/B family of protein toxins
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Leis, Susanne, Spindler, Jenny, Reiter, Jochen, Breinig, Frank, Schmitt, Manfred J., Schmitt, Manfred J., editor, and Schaffrath, Raffael, editor
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- 2005
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157. ExoU: A cytotoxin delivered by the type III secretion system of Pseudomonas aeruginosa
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Rabin, Shira D.P., Hauser, Alan R., Schmitt, Manfred J., editor, and Schaffrath, Raffael, editor
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- 2005
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158. Molecular analysis of the LTR retrotransposon Ylt1 from the genome of dimorphic fungus Yarrowia lipolytica
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Kovalchuk, Andriy, Barth, Gerold, Hyman, Anthony, and Schaffrath, Raffael
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Tyl6, Yarrowia lipolytica, Ylt1, retrotransposon, transposable elements ,Tyl6, Yarrowia lipolytica, Ylt1, mobile genetische Elemente, retrotransposon ,ddc:570 ,Molekularbiologie ,Retrotransposon ,Transponierbares Element ,Yarrowia lipolytica - Abstract
The retrotransposon Ylt1 was described previously from the genome of the dimorphic fungus Yarrowia lipolytica. Remarkably, Ylt1 is currently the largest LTR retrotransposon reported from fungal genomes. However, little was known about its biology and its interactions with host genome. So, the aim of this work was the characterization of properties of Ylt1.Analysis of proteins encoded by Ylt1 (Gag protein and integrase) was carried out during this work. To enable their detection, both proteins were tagged with HA epitopes. The sizes of Gag protein and putative precursors of Gag protein and integrase were estimated, and a model for the proteolytic processing of the polyprotein of Ylt1 was proposed. It was shown that Gag protein of Ylt1 is about 2-fold larger than Gag proteins of other studied yeast retrotransposons. An analysis of Ylt1 expression was also performed. Production of the Ylt1 Gag protein under different conditions was analyzed by Western blotting. Expression of Ylt1 occurred on all tested carbon sources. The amount of Ylt1 decreased rapidly upon transition to stationary growth phase, in the presence of copper sulfate and under heat shock conditions. It is suggested that Ylt1 is expressed in actively growing cells, whereas stress conditions have a negative impact on its expression. Such expression pattern was not previously reported for other yeast retrotransposons. Activity of Ylt1 in vivo was characterized using an Ylt1 elements tagged with SUC2 gene of Saccharomyces cerevisiae. Mobilization of the marked Ylt1 element and its transposition from autonomous plasmid into host genome was observed in performed experiments. Obtained results strongly support the idea that Ylt1 is transpositionally active. Formation of tandem repeats by newly inserted Ylt1 elements was observed in several cases. It is suggested that integrase function was affected in this case, and that the integration was mediated by homologous recombination instead. Analysis of the Ylt1 insertion specificity and of the Ylt1 distribution in the genome of Y. lipolytica E150 was done. The remarkable sequence specificity of Ylt1 insertions, which is unusual for LTR retrotransposons, was revealed during this analysis. Also, it was shown that Ylt1 insertions are found mainly in intergenic regions, often at a significant distance (>500 bp) from the next reading frame. No association of Ylt1 insertions with tRNA genes was observed. Searches for Ylt1-related elements in the Y. lipolytica genome database were performed. The novel Ty3/gypsy element Tyl6 was found in the genome of Y. lipolytica E150. The sequence analysis of this element was carried out. It was shown that structural properties of Tyl6 resemble the properties of the Ty3 element of S. cerevisiae. However, two reading frames of Tyl6 (gag and pol) are separated by -1 frame-shift, which was not previously reported for retrotransposons of hemiascomycetous yeasts. Phylogenetic analysis placed Tyl6 within chromoviruses, and the Tse3 element of S. exiguus was shown to be the closest relative of Tyl6. The distribution of Tyl6 among Y. lipolytica strains was analyzed. Interestingly, the novel element was found only in strains derived from the strain YB423-12. The strains of independent origin included in the analysis were shown to be Tyl6-free. The same distribution was previously reported for the retrotransposon Ylt1 and for the DNA transposon Mutyl. Two models of the evolution of transposable elements in Y. lipolytica genome were proposed based on these results.
- Published
- 2005
159. AtELP4 a subunit of the Elongator complex in Arabidopsis , mediates cell proliferation and dorsoventral polarity during leaf morphogenesis.
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Jun SE, Cho KH, Manzoor MA, Hwang TY, Kim YS, Schaffrath R, and Kim GT
- Abstract
The Elongator complex in eukaryotes has conserved tRNA modification functions and contributes to various physiological processes such as transcriptional control, DNA replication and repair, and chromatin accessibility. ARABIDOPSIS ELONGATOR PROTEIN 4 (AtELP4) is one of the six subunits (AtELP1-AtELP6) in Arabidopsis Elongator. In addition, there is an Elongator-associated protein, DEFORMED ROOTS AND LEAVES 1 (DRL1), whose homolog in yeast (Kti12) binds tRNAs. In this study, we explored the functions of AtELP4 in plant-specific aspects such as leaf morphogenesis and evolutionarily conserved ones between yeast and Arabidopsis . ELP4 comparison between yeast and Arabidopsis revealed that plant ELP4 possesses not only a highly conserved P-loop ATPase domain but also unknown plant-specific motifs. ELP4 function is partially conserved between Arabidopsis and yeast in the growth sensitivity toward caffeine and elevated cultivation temperature. Either single Atelp4 or drl1-102 mutants and double Atelp4 drl1-102 mutants exhibited a reduction in cell proliferation and changed the adaxial-abaxial polarity of leaves. In addition, the single Atelp4 and double Atelp4 drl1-102 mutants showed remarkable downward curling at the whole part of leaf blades in contrast to wild-type leaf blades. Furthermore, our genetic study revealed that AtELP4 might epistatically act on DRL1 in the regulation of cell proliferation and dorsoventral polarity in leaves. Taken together, we suggest that AtELP4 as part of the plant Elongator complex may act upstream of a regulatory pathway for adaxial-abaxial polarity and cell proliferation during leaf development., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Jun, Cho, Manzoor, Hwang, Kim, Schaffrath and Kim.)
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- 2022
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160. E2/E3-independent ubiquitin-like protein conjugation by Urm1 is directly coupled to cysteine persulfidation.
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Ravichandran KE, Kaduhr L, Skupien-Rabian B, Shvetsova E, Sokołowski M, Krutyhołowa R, Kwasna D, Brachmann C, Lin S, Guzman Perez S, Wilk P, Kösters M, Grudnik P, Jankowska U, Leidel SA, Schaffrath R, and Glatt S
- Subjects
- Anticodon, Carrier Proteins metabolism, Cysteine, Peroxiredoxins, Sulfur metabolism, Ubiquitin metabolism, Ubiquitins metabolism
- Abstract
Post-translational modifications by ubiquitin-like proteins (UBLs) are essential for nearly all cellular processes. Ubiquitin-related modifier 1 (Urm1) is a unique UBL, which plays a key role in tRNA anticodon thiolation as a sulfur carrier protein (SCP) and is linked to the noncanonical E1 enzyme Uba4 (ubiquitin-like protein activator 4). While Urm1 has also been observed to conjugate to target proteins like other UBLs, the molecular mechanism of its attachment remains unknown. Here, we reconstitute the covalent attachment of thiocarboxylated Urm1 to various cellular target proteins in vitro, revealing that, unlike other known UBLs, this process is E2/E3-independent and requires oxidative stress. Furthermore, we present the crystal structures of the peroxiredoxin Ahp1 before and after the covalent attachment of Urm1. Surprisingly, we show that urmylation is accompanied by the transfer of sulfur to cysteine residues in the target proteins, also known as cysteine persulfidation. Our results illustrate the role of the Uba4-Urm1 system as a key evolutionary link between prokaryotic SCPs and the UBL modifications observed in modern eukaryotes., (© 2022 The Authors. Published under the terms of the CC BY NC ND 4.0 license.)
- Published
- 2022
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161. A novel DPH5-related diphthamide-deficiency syndrome causing embryonic lethality or profound neurodevelopmental disorder.
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Shankar SP, Grimsrud K, Lanoue L, Egense A, Willis B, Hörberg J, AlAbdi L, Mayer K, Ütkür K, Monaghan KG, Krier J, Stoler J, Alnemer M, Shankar PR, Schaffrath R, Alkuraya FS, Brinkmann U, Eriksson LA, Lloyd K, and Rauen KA
- Published
- 2022
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162. Translational fidelity and growth of Arabidopsis require stress-sensitive diphthamide biosynthesis.
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Zhang H, Quintana J, Ütkür K, Adrian L, Hawer H, Mayer K, Gong X, Castanedo L, Schulten A, Janina N, Peters M, Wirtz M, Brinkmann U, Schaffrath R, and Krämer U
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- Animals, Histidine analogs & derivatives, Histidine metabolism, Humans, Mammals metabolism, Mice, Proteins metabolism, Saccharomyces cerevisiae metabolism, Arabidopsis genetics, Arabidopsis metabolism, Saccharomyces cerevisiae Proteins metabolism
- Abstract
Diphthamide, a post-translationally modified histidine residue of eukaryotic TRANSLATION ELONGATION FACTOR2 (eEF2), is the human host cell-sensitizing target of diphtheria toxin. Diphthamide biosynthesis depends on the 4Fe-4S-cluster protein Dph1 catalyzing the first committed step, as well as Dph2 to Dph7, in yeast and mammals. Here we show that diphthamide modification of eEF2 is conserved in Arabidopsis thaliana and requires AtDPH1. Ribosomal -1 frameshifting-error rates are increased in Arabidopsis dph1 mutants, similar to yeast and mice. Compared to the wild type, shorter roots and smaller rosettes of dph1 mutants result from fewer formed cells. TARGET OF RAPAMYCIN (TOR) kinase activity is attenuated, and autophagy is activated, in dph1 mutants. Under abiotic stress diphthamide-unmodified eEF2 accumulates in wild-type seedlings, most strongly upon heavy metal excess, which is conserved in human cells. In summary, our results suggest that diphthamide contributes to the functionality of the translational machinery monitored by plants to regulate growth., (© 2022. The Author(s).)
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- 2022
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163. Identifying Interaction Partners of Yeast Protein Disulfide Isomerases Using a Small Thiol-Reactive Cross-Linker: Implications for Secretory Pathway Proteostasis.
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Freije BJ, Freije WM, Do TU, Adkins GE, Bruch A, Hurtig JE, Morano KA, Schaffrath R, and West JD
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- Cross-Linking Reagents chemistry, Endoplasmic Reticulum drug effects, Endoplasmic Reticulum metabolism, Molecular Structure, Protein Disulfide-Isomerases antagonists & inhibitors, Protein Disulfide-Isomerases chemistry, Proteolysis drug effects, Proteostasis drug effects, Sulfhydryl Compounds chemistry, Sulfones pharmacology, Cross-Linking Reagents metabolism, Protein Disulfide-Isomerases metabolism, Saccharomyces cerevisiae enzymology, Sulfhydryl Compounds metabolism
- Abstract
Protein disulfide isomerases (PDIs) function in forming the correct disulfide bonds in client proteins, thereby aiding the folding of proteins that enter the secretory pathway. Recently, several PDIs have been identified as targets of organic electrophiles, yet the client proteins of specific PDIs remain largely undefined. Here, we report that PDIs expressed in Saccharomyces cerevisiae are targets of divinyl sulfone (DVSF) and other thiol-reactive protein cross-linkers. Using DVSF, we identified the interaction partners that were cross-linked to Pdi1 and Eug1, finding that both proteins form cross-linked complexes with other PDIs, as well as vacuolar hydrolases, proteins involved in cell wall biosynthesis and maintenance, and many ER proteostasis factors involved ER stress signaling and ER-associated protein degradation (ERAD). The latter discovery prompted us to examine the effects of DVSF on ER quality control, where we found that DVSF inhibits the degradation of the ERAD substrate CPY*, in addition to covalently modifying Ire1 and blocking the activation of the unfolded protein response. Our results reveal that DVSF targets many proteins within the ER proteostasis network and suggest that these proteins may be suitable targets for covalent therapeutic development in the future.
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- 2022
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164. Urm1, not quite a ubiquitin-like modifier?
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Kaduhr L, Brachmann C, Ravichandran KE, West JD, Glatt S, and Schaffrath R
- Abstract
Ubiquitin related modifier 1 (Urm1) is a unique eukaryotic member of the ubiquitin-fold (UbF) protein family and conserved from yeast to humans. Urm1 is dual-functional, acting both as a sulfur carrier for thiolation of tRNA anticodons and as a protein modifier in a lysine-directed Ub-like conjugation also known as urmylation. Although Urm1 conjugation coincides with oxidative stress and targets proteins like 2-Cys peroxiredoxins from yeast (Ahp1) and fly (Prx5), it was unclear how urmylation proceeds molecularly and whether it is affected by the activity of these antioxidant enzymes. An in-depth study of Ahp1 urmylation in yeast from our laboratory (Brachmann et al. , 2020) uncovered that promiscuous lysine target sites and specific redox requirements determine the Urm1 acceptor activity of the peroxiredoxin. The results clearly show that the dimer interface and the 2-Cys based redox-active centers of Ahp1 are affecting the Urm1 conjugation reaction. Together with in vivo assays demonstrating that high organic peroxide concentrations can prevent Ahp1 from being urmylated, Brachmann et al. provide insights into a potential link between Urm1 utilization and oxidant defense of cells. Here, we highlight these major findings and discuss wider implications with regards to an emerging link between Urm1 conjugation and redox biology. Moreover, from these studies we propose to redefine our perspective on Urm1 and the molecular nature of urmylation, a post-translational conjugation that may not be that ubiquitin-like after all., Competing Interests: Conflict of Interest: The authors declare no conflict of interest with respect to this work., (Copyright: © 2021 Kaduhr et al.)
- Published
- 2021
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165. Role of SSD1 in Phenotypic Variation of Saccharomyces cerevisiae Strains Lacking DEG1 -Dependent Pseudouridylation.
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Khonsari B, Klassen R, and Schaffrath R
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- Biological Variation, Population, Intramolecular Transferases genetics, Intramolecular Transferases deficiency, RNA Processing, Post-Transcriptional genetics, RNA, Fungal genetics, RNA, Fungal metabolism, RNA, Transfer genetics, RNA, Transfer metabolism, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae metabolism, Saccharomyces cerevisiae Proteins genetics, Saccharomyces cerevisiae Proteins metabolism
- Abstract
Yeast phenotypes associated with the lack of wobble uridine (U
34 ) modifications in tRNA were shown to be modulated by an allelic variation of SSD1 , a gene encoding an mRNA-binding protein. We demonstrate that phenotypes caused by the loss of Deg1-dependent tRNA pseudouridylation are similarly affected by SSD1 allelic status. Temperature sensitivity and protein aggregation are elevated in deg1 mutants and further increased in the presence of the ssd1-d allele, which encodes a truncated form of Ssd1. In addition, chronological lifespan is reduced in a deg1 ssd1-d mutant, and the negative genetic interactions of the U34 modifier genes ELP3 and URM1 with DEG1 are aggravated by ssd1-d . A loss of function mutation in SSD1 , ELP3 , and DEG1 induces pleiotropic and overlapping phenotypes, including sensitivity against target of rapamycin (TOR) inhibitor drug and cell wall stress by calcofluor white. Additivity in ssd1 deg1 double mutant phenotypes suggests independent roles of Ssd1 and tRNA modifications in TOR signaling and cell wall integrity. However, other tRNA modification defects cause growth and drug sensitivity phenotypes, which are not further intensified in tandem with ssd1-d . Thus, we observed a modification-specific rather than general effect of SSD1 status on phenotypic variation in tRNA modification mutants. Our results highlight how the cellular consequences of tRNA modification loss can be influenced by protein targeting specific mRNAs.- Published
- 2021
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166. Induction of protein aggregation and starvation response by tRNA modification defects.
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Klassen R, Bruch A, and Schaffrath R
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- Anticodon genetics, Codon genetics, Nucleic Acid Conformation, Protein Biosynthesis genetics, RNA Processing, Post-Transcriptional genetics, Protein Aggregates genetics, RNA, Transfer genetics, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae Proteins genetics
- Abstract
Posttranscriptional modifications of anticodon loops contribute to the decoding efficiency of tRNAs by supporting codon recognition and loop stability. Consistently, strong synthetic growth defects are observed in yeast strains simultaneously lacking distinct anticodon loop modifications. These phenotypes are accompanied by translational inefficiency of certain mRNAs and disturbed protein homeostasis resulting in accumulation of protein aggregates. Different combinations of anticodon loop modification defects were shown to affect distinct tRNAs but provoke common transcriptional changes that are reminiscent of the cellular response to nutrient starvation. Multiple mechanisms may be involved in mediating inadequate starvation response upon loss of critical tRNA modifications. Recent evidence suggests protein aggregate induction to represent one such trigger.
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- 2020
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167. Diphthamide-deficiency syndrome: a novel human developmental disorder and ribosomopathy.
- Author
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Hawer H, Mendelsohn BA, Mayer K, Kung A, Malhotra A, Tuupanen S, Schleit J, Brinkmann U, and Schaffrath R
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- Cell Line, Developmental Disabilities metabolism, Developmental Disabilities pathology, Heart Defects, Congenital metabolism, Heart Defects, Congenital pathology, Histidine deficiency, Histidine metabolism, Humans, Infant, Male, Megalencephaly metabolism, Megalencephaly pathology, Proteins metabolism, Saccharomyces cerevisiae, Syndrome, Developmental Disabilities genetics, Heart Defects, Congenital genetics, Histidine analogs & derivatives, Loss of Function Mutation, Megalencephaly genetics, Proteins genetics, Ribosomes metabolism
- Abstract
We describe a novel type of ribosomopathy that is defined by deficiency in diphthamidylation of translation elongation factor 2. The ribosomopathy was identified by correlating phenotypes and biochemical properties of previously described patients with diphthamide biosynthesis gene 1 (DPH1) deficiencies with a new patient that carried inactivating mutations in both alleles of the human diphthamide biosynthesis gene 2 (DPH2). The human DPH1 syndrome is an autosomal recessive disorder associated with developmental delay, abnormal head circumference (microcephaly or macrocephaly), short stature, and congenital heart disease. It is defined by variants with reduced functionality of the DPH1 gene observed so far predominantly in consanguineous homozygous patients carrying identical mutant alleles of DPH1. Here we report a child with a very similar phenotype carrying biallelic variants of the human DPH2. The gene products DPH1 and DPH2 are components of a heterodimeric enzyme complex that mediates the first step of the posttranslational diphthamide modification on the nonredundant eukaryotic translation elongation factor 2 (eEF2). Diphthamide deficiency was shown to reduce the accuracy of ribosomal protein biosynthesis. Both DPH2 variants described here severely impair diphthamide biosynthesis as demonstrated in human and yeast cells. This is the first report of a patient carrying compound heterozygous DPH2 loss-of-function variants with a DPH1 syndrome-like phenotype and implicates diphthamide deficiency as the root cause of this patient's clinical phenotype as well as of DPH1-syndrome. These findings define "diphthamide-deficiency syndrome" as a special ribosomopathy due to reduced functionality of components of the cellular machinery for eEF2-diphthamide synthesis.
- Published
- 2020
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168. Eukaryotic life without tQCUG: the role of Elongator-dependent tRNA modifications in Dictyostelium discoideum.
- Author
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Schäck MA, Jablonski KP, Gräf S, Klassen R, Schaffrath R, Kellner S, and Hammann C
- Subjects
- Anticodon chemistry, Anticodon metabolism, Codon, Dictyostelium metabolism, Gene Deletion, Glutamine, Histone Acetyltransferases genetics, Histone Acetyltransferases metabolism, Mutation, Nucleosides chemistry, Phylogeny, Protein Biosynthesis, Protozoan Proteins classification, Protozoan Proteins genetics, Protozoan Proteins metabolism, Uridine metabolism, Dictyostelium genetics, RNA, Transfer metabolism
- Abstract
In the Elongator-dependent modification pathway, chemical modifications are introduced at the wobble uridines at position 34 in transfer RNAs (tRNAs), which serve to optimize codon translation rates. Here, we show that this three-step modification pathway exists in Dictyostelium discoideum, model of the evolutionary superfamily Amoebozoa. Not only are previously established modifications observable by mass spectrometry in strains with the most conserved genes of each step deleted, but also additional modifications are detected, indicating a certain plasticity of the pathway in the amoeba. Unlike described for yeast, D. discoideum allows for an unconditional deletion of the single tQCUG gene, as long as the Elongator-dependent modification pathway is intact. In gene deletion strains of the modification pathway, protein amounts are significantly reduced as shown by flow cytometry and Western blotting, using strains expressing different glutamine leader constructs fused to GFP. Most dramatic are these effects, when the tQCUG gene is deleted, or Elp3, the catalytic component of the Elongator complex is missing. In addition, Elp3 is the most strongly conserved protein of the modification pathway, as our phylogenetic analysis reveals. The implications of this observation are discussed with respect to the evolutionary age of the components acting in the Elongator-dependent modification pathway., (© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.)
- Published
- 2020
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169. Misactivation of multiple starvation responses in yeast by loss of tRNA modifications.
- Author
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Bruch A, Laguna T, Butter F, Schaffrath R, and Klassen R
- Subjects
- Autophagy, Glucose metabolism, Mutation, Nitrogen metabolism, RNA, Transfer genetics, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins genetics, Saccharomyces cerevisiae Proteins metabolism, Gene Expression Regulation, Fungal, Glucose deficiency, Nitrogen deficiency, RNA, Transfer metabolism
- Abstract
Previously, combined loss of different anticodon loop modifications was shown to impair the function of distinct tRNAs in Saccharomyces cerevisiae. Surprisingly, each scenario resulted in shared cellular phenotypes, the basis of which is unclear. Since loss of tRNA modification may evoke transcriptional responses, we characterized global transcription patterns of modification mutants with defects in either tRNAGlnUUG or tRNALysUUU function. We observe that the mutants share inappropriate induction of multiple starvation responses in exponential growth phase, including derepression of glucose and nitrogen catabolite-repressed genes. In addition, autophagy is prematurely and inadequately activated in the mutants. We further demonstrate that improper induction of individual starvation genes as well as the propensity of the tRNA modification mutants to form protein aggregates are diminished upon overexpression of tRNAGlnUUG or tRNALysUUU, the tRNA species that lack the modifications of interest. Hence, our data suggest that global alterations in mRNA translation and proteostasis account for the transcriptional stress signatures that are commonly triggered by loss of anticodon modifications in different tRNAs., (© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.)
- Published
- 2020
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170. Loss of Elongator- and KEOPS-Dependent tRNA Modifications Leads to Severe Growth Phenotypes and Protein Aggregation in Yeast.
- Author
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Pollo-Oliveira L, Klassen R, Davis N, Ciftci A, Bacusmo JM, Martinelli M, DeMott MS, Begley TJ, Dedon PC, Schaffrath R, and de Crécy-Lagard V
- Subjects
- Anticodon genetics, Anticodon metabolism, DNA-Binding Proteins metabolism, Histone Acetyltransferases metabolism, Nucleic Acid Conformation, Phenotype, Protein Aggregates physiology, Protein Biosynthesis genetics, Protein Biosynthesis physiology, Proteins genetics, Proteomics methods, RNA, Transfer genetics, Saccharomyces cerevisiae growth & development, Saccharomyces cerevisiae metabolism, Saccharomyces cerevisiae Proteins metabolism, Thermodynamics, Thiouridine analogs & derivatives, Thiouridine chemistry, DNA-Binding Proteins genetics, Histone Acetyltransferases genetics, RNA, Transfer metabolism, Saccharomyces cerevisiae Proteins genetics
- Abstract
Modifications found in the Anticodon Stem Loop (ASL) of tRNAs play important roles in regulating translational speed and accuracy. Threonylcarbamoyl adenosine (t
6 A37) and 5-methoxycarbonyl methyl-2-thiouridine (mcm5 s2 U34) are critical ASL modifications that have been linked to several human diseases. The model yeast Saccharomyces cerevisiae is viable despite the absence of both modifications, growth is however greatly impaired. The major observed consequence is a subsequent increase in protein aggregates and aberrant morphology. Proteomic analysis of the t6 A-deficient strain ( sua5 mutant) revealed a global mistranslation leading to protein aggregation without regard to physicochemical properties or t6 A-dependent or biased codon usage in parent genes. However, loss of sua5 led to increased expression of soluble proteins for mitochondrial function, protein quality processing/trafficking, oxidative stress response, and energy homeostasis. These results point to a global function for t6 A in protein homeostasis very similar to mcm5 /s2 U modifications.- Published
- 2020
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171. Redox requirements for ubiquitin-like urmylation of Ahp1, a 2-Cys peroxiredoxin from yeast.
- Author
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Brachmann C, Kaduhr L, Jüdes A, Ravichandran KE, West JD, Glatt S, and Schaffrath R
- Subjects
- Catalytic Domain, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Fungal, Models, Molecular, Oxidation-Reduction, Peroxides metabolism, Peroxiredoxins chemistry, Peroxiredoxins genetics, Protein Conformation, Protein Multimerization, Saccharomyces cerevisiae genetics, Mutation, Peroxiredoxins metabolism, Saccharomyces cerevisiae enzymology, Saccharomyces cerevisiae Proteins metabolism
- Abstract
The yeast peroxiredoxin Ahp1, like related anti-oxidant enzymes in other species, undergoes urmylation, a lysine-directed conjugation to ubiquitin-like modifier Urm1. Ahp1 assembles into a homodimer that detoxifies peroxides via forming intersubunit disulfides between peroxidatic and resolving cysteines that are subsequently reduced by the thioredoxin system. Although urmylation coincides with oxidative stress, it is unclear how this modification happens on a molecular level and whether it affects peroxiredoxin activity. Here, we report that thioredoxin mutants decrease Ahp1 urmylation in yeast and each subunit of the oxidized Ahp1 dimer is modified by Urm1 suggesting coupling of urmylation to dimerization. Consistently, Ahp1 mutants unable to form dimers, fail to be urmylated as do mutants that lack the peroxidatic cysteine. Moreover, Ahp1 urmylation involves at least two lysine residues close to the catalytic cysteines and can be prevented in yeast cells exposed to high organic peroxide concentrations. Our results elucidate redox requirements and molecular determinants critical for Ahp1 urmylation, thus providing insights into a potential link between oxidant defense and Urm1 utilization in cells., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2020
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172. Absolute Quantification of Noncoding RNA by Microscale Thermophoresis.
- Author
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Jacob D, Thüring K, Galliot A, Marchand V, Galvanin A, Ciftci A, Scharmann K, Stock M, Roignant JY, Leidel SA, Motorin Y, Schaffrath R, Klassen R, and Helm M
- Subjects
- Fluorescence, RNA, Untranslated chemistry
- Abstract
Accurate quantification of the copy numbers of noncoding RNA has recently emerged as an urgent problem, with impact on fields such as RNA modification research, tissue differentiation, and others. Herein, we present a hybridization-based approach that uses microscale thermophoresis (MST) as a very fast and highly precise readout to quantify, for example, single tRNA species with a turnaround time of about one hour. We developed MST to quantify the effect of tRNA toxins and of heat stress and RNA modification on single tRNA species. A comparative analysis also revealed significant differences to RNA-Seq-based quantification approaches, strongly suggesting a bias due to tRNA modifications in the latter. Further applications include the quantification of rRNA as well as of polyA levels in cellular RNA., (© 2019 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.)
- Published
- 2019
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173. Collaboration of tRNA modifications and elongation factor eEF1A in decoding and nonsense suppression.
- Author
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Klassen R and Schaffrath R
- Subjects
- Epistasis, Genetic, Genes, Fungal, Genes, Reporter, Hot Temperature, Luciferases metabolism, Nonsense Mediated mRNA Decay genetics, Saccharomyces cerevisiae growth & development, Saccharomyces cerevisiae metabolism, Saccharomyces cerevisiae Proteins genetics, Codon, Nonsense genetics, Peptide Elongation Factor 1 metabolism, RNA, Transfer genetics, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae Proteins metabolism, Suppression, Genetic
- Abstract
Transfer RNA (tRNA) from all domains of life contains multiple modified nucleosides, the functions of which remain incompletely understood. Genetic interactions between tRNA modification genes in Saccharomyces cerevisiae suggest that different tRNA modifications collaborate to maintain translational efficiency. Here we characterize such collaborative functions in the ochre suppressor tRNA SUP4. We quantified ochre read-through efficiency in mutants lacking either of the 7 known modifications in the extended anticodon stem loop (G26-C48). Absence of U34, U35, A37, U47 and C48 modifications partially impaired SUP4 function. We systematically combined modification defects and scored additive or synergistic negative effects on SUP4 performance. Our data reveal different degrees of functional redundancy between specific modifications, the strongest of which was demonstrated for those occurring at positions U34 and A37. SUP4 activity in the absence of critical modifications, however, can be rescued in a gene dosage dependent fashion by TEF1 which encodes elongation factor eEF1A required for tRNA delivery to the ribosome. Strikingly, the rescue ability of higher-than-normal eEF1A levels extends to tRNA modification defects in natural non-suppressor tRNAs suggesting that elevated eEF1A abundance can partially compensate for functional defects induced by loss of tRNA modifications.
- Published
- 2018
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174. Sulfur transfer and activation by ubiquitin-like modifier system Uba4•Urm1 link protein urmylation and tRNA thiolation in yeast.
- Author
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Jüdes A, Bruch A, Klassen R, Helm M, and Schaffrath R
- Abstract
Urm1 is a unique dual-function member of the ubiquitin protein family and conserved from yeast to man. It acts both as a protein modifier in ubiquitin-like urmylation and as a sulfur donor for tRNA thiolation, which in concert with the Elongator pathway forms 5-methoxy-carbonyl-methyl-2-thio (mcm
5 s2 ) modified wobble uridines (U34) in anticodons. Using Saccharomyces cerevisiae as a model to study a relationship between these two functions, we examined whether cultivation temperature and sulfur supply previously implicated in the tRNA thiolation branch of the URM1 pathway also contribute to proper urmylation. Monitoring Urm1 conjugation, we found urmylation of the peroxiredoxin Ahp1 is suppressed either at elevated cultivation temperatures or under sulfur starvation. In line with this, mutants with sulfur transfer defects that are linked to enzymes (Tum1, Uba4) required for Urm1 activation by thiocarboxylation (Urm1-COSH) were found to maintain drastically reduced levels of Ahp1 urmylation and mcm5 s2 U34 modification. Moreover, as revealed by site specific mutagenesis, the S-transfer rhodanese domain (RHD) in the E1-like activator (Uba4) crucial for Urm1-COSH formation is critical but not essential for protein urmylation and tRNA thiolation. In sum, sulfur supply, transfer and activation chemically link protein urmylation and tRNA thiolation. These are features that distinguish the ubiquitin-like modifier system Uba4•Urm1 from canonical ubiquitin family members and will help elucidate whether, in addition to their mechanistic links, the protein and tRNA modification branches of the URM1 pathway may also relate in function to one another., Competing Interests: Conflict of interest: The authors declare there is no conflict of interest.- Published
- 2016
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175. Glutaredoxin GRXS17 Associates with the Cytosolic Iron-Sulfur Cluster Assembly Pathway.
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Iñigo S, Durand AN, Ritter A, Le Gall S, Termathe M, Klassen R, Tohge T, De Coninck B, Van Leene J, De Clercq R, Cammue BP, Fernie AR, Gevaert K, De Jaeger G, Leidel SA, Schaffrath R, Van Lijsebettens M, Pauwels L, and Goossens A
- Subjects
- Arabidopsis genetics, Arabidopsis metabolism, Arabidopsis Proteins genetics, DNA Damage, Gene Expression Regulation, Plant, Glutaredoxins genetics, Immunoblotting, Mutation, Plant Leaves genetics, Plant Leaves metabolism, Plant Roots genetics, Plant Roots metabolism, Plants, Genetically Modified, Protein Binding, Reverse Transcriptase Polymerase Chain Reaction, Xanthine Dehydrogenase genetics, Xanthine Dehydrogenase metabolism, Arabidopsis Proteins metabolism, Biosynthetic Pathways, Cytosol metabolism, Glutaredoxins metabolism, Iron-Sulfur Proteins metabolism
- Abstract
Cytosolic monothiol glutaredoxins (GRXs) are required in iron-sulfur (Fe-S) cluster delivery and iron sensing in yeast and mammals. In plants, it is unclear whether they have similar functions. Arabidopsis (Arabidopsis thaliana) has a sole class II cytosolic monothiol GRX encoded by GRXS17 Here, we used tandem affinity purification to establish that Arabidopsis GRXS17 associates with most known cytosolic Fe-S assembly (CIA) components. Similar to mutant plants with defective CIA components, grxs17 loss-of-function mutants showed some degree of hypersensitivity to DNA damage and elevated expression of DNA damage marker genes. We also found that several putative Fe-S client proteins directly bind to GRXS17, such as XANTHINE DEHYDROGENASE1 (XDH1), involved in the purine salvage pathway, and CYTOSOLIC THIOURIDYLASE SUBUNIT1 and CYTOSOLIC THIOURIDYLASE SUBUNIT2, both essential for the 2-thiolation step of 5-methoxycarbonylmethyl-2-thiouridine (mcm
5 s2 U) modification of tRNAs. Correspondingly, profiling of the grxs17-1 mutant pointed to a perturbed flux through the purine degradation pathway and revealed that it phenocopied mutants in the elongator subunit ELO3, essential for the mcm5 tRNA modification step, although we did not find XDH1 activity or tRNA thiolation to be markedly reduced in the grxs17-1 mutant. Taken together, our data suggest that plant cytosolic monothiol GRXs associate with the CIA complex, as in other eukaryotes, and contribute to, but are not essential for, the correct functioning of client Fe-S proteins in unchallenged conditions., (© 2016 American Society of Plant Biologists. All Rights Reserved.)- Published
- 2016
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176. Comparative analysis of the conserved functions of Arabidopsis DRL1 and yeast KTI12.
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Jun SE, Cho KH, Hwang JY, Abdel-Fattah W, Hammermeister A, Schaffrath R, Bowman JL, and Kim GT
- Subjects
- Adaptor Proteins, Signal Transducing chemistry, Amino Acid Sequence, Arabidopsis growth & development, Arabidopsis metabolism, Arabidopsis Proteins chemistry, Caffeine pharmacology, Conserved Sequence, GTP-Binding Proteins chemistry, Genetic Complementation Test, Molecular Sequence Data, Saccharomyces cerevisiae drug effects, Saccharomyces cerevisiae metabolism, Saccharomyces cerevisiae Proteins chemistry, Stress, Physiological, Adaptor Proteins, Signal Transducing physiology, Arabidopsis genetics, Arabidopsis Proteins physiology, GTP-Binding Proteins physiology, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae Proteins physiology
- Abstract
Patterning of the polar axis during the early leaf developmental stage is established by cell-to-cell communication between the shoot apical meristem (SAM) and the leaf primordia. In a previous study, we showed that the DRL1 gene, which encodes a homolog of the Elongator-associated protein KTI12 of yeast, acts as a positive regulator of adaxial leaf patterning and shoot meristem activity. To determine the evolutionally conserved functions of DRL1, we performed a comparison of the deduced amino acid sequence of DRL1 and its yeast homolog, KTI12, and found that while overall homology was low, well-conserved domains were presented. DRL1 contained two conserved plant-specific domains. Expression of the DRL1 gene in a yeast KTI12-deficient yeast mutant suppressed the growth retardation phenotype, but did not rescue the caffeine sensitivity, indicating that the role of Arabidopsis Elongator-associated protein is partially conserved with yeast KTI12, but may have changed between yeast and plants in response to caffeine during the course of evolution. In addition, elevated expression of DRL1 gene triggered zymocin sensitivity, while overexpression of KTI12 maintained zymocin resistance, indicating that the function of Arabidopsis DRL1 may not overlap with yeast KTI12 with regards to toxin sensitivity. In this study, expression analysis showed that class-I KNOX genes were downregulated in the shoot apex, and that YAB and KAN were upregulated in leaves of the Arabidopsis drl1-101 mutant. Our results provide insight into the communication network between the SAM and leaf primordia required for the establishment of leaf polarity by mediating histone acetylation or through other mechanisms.
- Published
- 2015
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177. Loss of wobble uridine modification in tRNA anticodons interferes with TOR pathway signaling.
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Scheidt V, Jüdes A, Bär C, Klassen R, and Schaffrath R
- Abstract
Previous work in yeast has suggested that modification of tRNAs, in particular uridine bases in the anticodon wobble position (U34), is linked to TOR (target of rapamycin) signaling. Hence, U34 modification mutants were found to be hypersensitive to TOR inhibition by rapamycin. To study whether this involves inappropriate TOR signaling, we examined interaction between mutations in TOR pathway genes ( tip41 ∆, sap190 ∆, ppm1 ∆, rrd1 ∆) and U34 modification defects ( elp3 ∆, kti 12∆, urm1 ∆, ncs2 ∆) and found the rapamycin hypersensitivity in the latter is epistatic to drug resistance of the former. Epistasis, however, is abolished in tandem with a gln3 ∆ deletion, which inactivates transcription factor Gln3 required for TOR-sensitive activation of NCR (nitrogen catabolite repression) genes. In line with nuclear import of Gln3 being under control of TOR and dephosphorylation by the Sit4 phosphatase, we identify novel TOR-sensitive sit4 mutations that confer rapamycin resistance and importantly, mislocalise Gln3 when TOR is inhibited. This is similar to gln3 ∆ cells, which abolish the rapamycin hypersensitivity of U34 modification mutants, and suggests TOR deregulation due to tRNA undermodification operates through Gln3. In line with this, loss of U34 modifications ( elp3 ∆, urm1 ∆) enhances nuclear import of and NCR gene activation ( MEP2 , GAP1 ) by Gln3 when TOR activity is low. Strikingly, this stimulatory effect onto Gln3 is suppressed by overexpression of tRNAs that usually carry the U34 modifications. Collectively, our data suggest that proper TOR signaling requires intact tRNA modifications and that loss of U34 modifications impinges on the TOR-sensitive NCR branch via Gln3 misregulation., Competing Interests: Conflict of interest: The authors declare there is no conflict of interest.
- Published
- 2014
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178. Decoding the biosynthesis and function of diphthamide, an enigmatic modification of translation elongation factor 2 (EF2)
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Schaffrath R and Stark MJ
- Abstract
Diphthamide is a highly conserved modification of archaeal and eukaryal translation elongation factor 2 (EF2) and yet why cells need EF2 to contain diphthamide is unclear. In yeast, the first steps of diphthamide synthesis and the genes ( DPH1-DPH5 ) required to form the intermediate diphthine are well-documented. However, the last step, amidation of diphthine to diphthamide, had largely been ill-defined. Remarkably, through mining genome-wide synthetic gene array (SGA) and chemical genomics databases, recent studies by Uthman et al. [PLoS Genetics (2013) 9, e1003334] and Su et al. [Proc. Natl. Acad. Sci. USA (2012) 109, 19983-19987] have identified two more diphthamide players, DPH6 and DPH7 . Consistent with roles in the amidation step, dph6 and dph7 deletion strains fail to complete diphthamide synthesis and accumulate diphthine-modified EF2. In contrast to Dph6, the catalytically relevant amidase, Dph7 appears to be regulatory. As shown by Uthman et al. , it promotes dissociation of diphthine synthase (Dph5) from EF2, allowing diphthine amidation by Dph6 to occur and thereby coupling diphthine synthesis to the terminal step in the pathway. Remarkably, the study by Uthman et al . suggests that Dph5 has a novel role as an EF2 inhibitor that affects cell growth when diphthamide synthesis is blocked or incomplete and, importantly, shows that diphthamide promotes the accuracy of EF2 performance during translation., Competing Interests: Conflict of interest: The authors declare they have no competing financial interests or other conflict of interests.
- Published
- 2014
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179. Determinants of eukaryal cell killing by the bacterial ribotoxin PrrC.
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Meineke B, Schwer B, Schaffrath R, and Shuman S
- Subjects
- Alanine genetics, Amino Acid Sequence, Bacterial Proteins metabolism, Bacterial Toxins genetics, Escherichia coli Proteins genetics, Molecular Sequence Data, Mutagenesis, Protein Structure, Tertiary, RNA, Transfer chemistry, RNA, Transfer, Lys metabolism, Ribonucleases genetics, Saccharomyces cerevisiae metabolism, Sequence Homology, Amino Acid, Structure-Activity Relationship, Bacterial Toxins chemistry, Bacterial Toxins metabolism, Escherichia coli Proteins chemistry, Escherichia coli Proteins metabolism, Ribonucleases chemistry, Ribonucleases metabolism
- Abstract
tRNA damage inflicted by the Escherichia coli anticodon nuclease PrrC (EcoPrrC) underlies an antiviral response to phage T4 infection. PrrC homologs are present in many bacterial proteomes, though their biological activities are uncharted. PrrCs consist of two domains: an N-terminal NTPase module related to the ABC family and a distinctive C-terminal ribonuclease module. In this article, we report that the expression of EcoPrrC in budding yeast is fungicidal, signifying that PrrC is toxic in a eukaryon in the absence of other bacterial or viral proteins. Whereas Streptococcus PrrC is also toxic in yeast, Neisseria and Xanthomonas PrrCs are not. Via analysis of the effects of 118 mutations on EcoPrrC toxicity in yeast, we identified 22 essential residues in the NTPase domain and 11 in the nuclease domain. Overexpressing PrrCs with mutations in the NTPase active site ameliorated the toxicity of wild-type EcoPrrC. Our findings support a model in which EcoPrrC toxicity is contingent on head-to-tail dimerization of the NTPase domains to form two composite NTP phosphohydrolase sites. Comparisons of EcoPrrC activity in a variety of yeast genetic backgrounds, and the rescuing effects of tRNA overexpression, implicate tRNA(Lys(UUU)) as a target of EcoPrrC toxicity in yeast.
- Published
- 2011
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180. Elongator function in tRNA wobble uridine modification is conserved between yeast and plants.
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Mehlgarten C, Jablonowski D, Wrackmeyer U, Tschitschmann S, Sondermann D, Jäger G, Gong Z, Byström AS, Schaffrath R, and Breunig KD
- Subjects
- Acetyltransferases genetics, Acetyltransferases metabolism, Animals, Arabidopsis enzymology, Arabidopsis genetics, Arabidopsis Proteins genetics, Genetic Complementation Test, Histone Acetyltransferases genetics, Membrane Proteins genetics, Membrane Proteins metabolism, Molecular Structure, RNA, Transfer chemistry, RNA, Transfer genetics, Recombinant Fusion Proteins genetics, Recombinant Fusion Proteins metabolism, Saccharomyces cerevisiae enzymology, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae Proteins genetics, Uridine chemistry, Uridine metabolism, Arabidopsis Proteins metabolism, Histone Acetyltransferases metabolism, RNA, Transfer metabolism, Saccharomyces cerevisiae Proteins metabolism, Uridine genetics
- Abstract
Based on studies in yeast and mammalian cells the Elongator complex has been implicated in functions as diverse as histone acetylation, polarized protein trafficking and tRNA modification. Here we show that Arabidopsis mutants lacking the Elongator subunit AtELP3/ELO3 have a defect in tRNA wobble uridine modification. Moreover, we demonstrate that yeast elp3 and elp1 mutants expressing the respective Arabidopsis Elongator homologues AtELP3/ELO3 and AtELP1/ELO2 assemble integer Elongator complexes indicating a high degree of structural conservation. Surprisingly, in vivo complementation studies based on Elongator-dependent tRNA nonsense suppression and zymocin tRNase toxin assays indicated that while AtELP1 rescued defects of a yeast elp1 mutant, the most conserved Elongator gene AtELP3, failed to complement an elp3 mutant. This lack of complementation is due to incompatibility with yeast ELP1 as coexpression of both plant genes in an elp1 elp3 yeast mutant restored Elongator's tRNA modification function in vivo. Similarly, AtELP1, not ScELP1 also supported partial complementation by yeast-plant Elp3 hybrids suggesting that AtElp1 has less stringent sequence requirements for Elp3 than ScElp1. We conclude that yeast and plant Elongator share tRNA modification roles and propose that this function might be conserved in Elongator from all eukaryotic kingdoms of life.
- Published
- 2010
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181. Elongator function depends on antagonistic regulation by casein kinase Hrr25 and protein phosphatase Sit4.
- Author
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Mehlgarten C, Jablonowski D, Breunig KD, Stark MJ, and Schaffrath R
- Subjects
- Antifungal Agents pharmacology, Casein Kinase I genetics, Codon, Nonsense, Drug Resistance, Fungal, Genes, Suppressor, Killer Factors, Yeast pharmacology, Mutation, Phosphorylation, Protein Phosphatase 2 genetics, Saccharomyces cerevisiae drug effects, Saccharomyces cerevisiae Proteins genetics, Suppression, Genetic, Casein Kinase I metabolism, Gene Expression Regulation, Histone Acetyltransferases metabolism, Peptide Elongation Factors metabolism, Protein Biosynthesis, Protein Phosphatase 2 metabolism, Saccharomyces cerevisiae physiology, Saccharomyces cerevisiae Proteins metabolism
- Abstract
In yeast, the role for the Elongator complex in tRNA anticodon modification is affected by phosphorylation of Elongator subunit Elp1. Thus, hyperphosphorylation of Elp1 due to inactivation of protein phosphatase Sit4 correlates with Elongator-minus phenotypes including resistance towards zymocin, a tRNase cleaving anticodons of Elongator-dependent tRNAs. Here we show that zymocin resistance of casein kinase hrr25 mutants associates with hypophosphorylation of Elp1 and that nonsense suppression by the Elongator-dependent SUP4 tRNA is abolished in hrr25 or sit4 mutants. Thus changes that perturb the evenly balanced ratio between hyper- and hypophosphorylated Elp1 forms present in wild-type cells lead to Elongator inactivation. Antagonistic roles for Hrr25 and Sit4 in Elongator function are further supported by our data that Sit4 inactivation is capable of restoring both zymocin sensitivity and normal ratios between the two Elp1 forms in hrr25 mutants. Hrr25 binds to Elongator in a fashion dependent on Elongator partner Kti12. Like sit4 mutants, overexpression of Kti12 triggers Elp1 hyperphosphorylation. Intriguingly, this effect of Kti12 is blocked by hrr25 mutations, which also show enhanced binding of Kti12 to Elongator. Collectively, our data suggest that rather than directly targeting Elp1, the Hrr25 kinase indirectly affects Elp1 phosphorylation states through control of Sit4-dependent dephosphorylation of Elp1.
- Published
- 2009
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182. tRNA and protein methylase complexes mediate zymocin toxicity in yeast.
- Author
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Studte P, Zink S, Jablonowski D, Bär C, von der Haar T, Tuite MF, and Schaffrath R
- Subjects
- Anticodon genetics, Anticodon metabolism, Drug Resistance, Fungal, Killer Factors, Yeast, Kluyveromyces metabolism, Mutation, Peptide Termination Factors genetics, Peptide Termination Factors metabolism, RNA, Transfer metabolism, Saccharomyces cerevisiae metabolism, Saccharomyces cerevisiae Proteins chemistry, Saccharomyces cerevisiae Proteins genetics, Suppression, Genetic, Uridine genetics, Uridine metabolism, tRNA Methyltransferases chemistry, tRNA Methyltransferases genetics, Gene Expression Regulation, Fungal, Mycotoxins pharmacology, RNA, Transfer genetics, Saccharomyces cerevisiae drug effects, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae Proteins metabolism, tRNA Methyltransferases metabolism
- Abstract
Modification of Saccharomyces cerevisiae tRNA anticodons at the wobble uridine (U34) position is required for tRNA cleavage by the zymocin tRNase killer toxin from Kluyveromyces lactis. Hence, U34 modification defects including lack of the U34 tRNA methyltransferase Trm9 protect against tRNA cleavage and zymocin. Using zymocin as a tool, we have identified toxin-resistant mutations in TRM9 that are likely to affect the U34 methylation reaction. Most strikingly, C-terminal truncations in Trm9 abolish interaction with Trm112, a protein shown to individually purify with Lys9 and two more methylases, Trm11 and Mtq2. Downregulation of a GAL1-TRM112 allele protects against zymocin whereas LYS9, TRM11 and MTQ2 are dosage suppressors of zymocin. Based on immune precipitation studies, the latter scenario correlates with competition for Trm112 and in excess, some of these Trm112 partners interfere with formation of the toxin-relevant Trm9.Trm112 complex. In contrast to trm11Delta or lys9Delta cells, trm112Delta and mtq2Delta null mutants are zymocin resistant. In line with the identified role that methylation of Sup45 by Mtq2 has for translation termination by the release factor dimer Sup45.Sup35, we observe that SUP45 overexpression and sup45 mutants suppress zymocin. Intriguingly, this suppression correlates with upregulated levels of tRNA species targeted by zymocin's tRNase activity.
- Published
- 2008
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183. A versatile partner of eukaryotic protein complexes that is involved in multiple biological processes: Kti11/Dph3.
- Author
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Bär C, Zabel R, Liu S, Stark MJ, and Schaffrath R
- Subjects
- Drug Resistance, Fungal, Histidine analogs & derivatives, Histidine pharmacology, Indenes pharmacology, Killer Factors, Yeast, Methyltransferases genetics, Methyltransferases metabolism, Mutation, Mycotoxins pharmacology, Peptide Elongation Factors genetics, Peptide Elongation Factors metabolism, Protein Binding, Protein Transport, RNA, Transfer genetics, RNA, Transfer metabolism, Repressor Proteins chemistry, Repressor Proteins genetics, Saccharomyces cerevisiae drug effects, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae Proteins chemistry, Saccharomyces cerevisiae Proteins genetics, Gene Expression Regulation, Fungal, Repressor Proteins metabolism, Saccharomyces cerevisiae metabolism, Saccharomyces cerevisiae Proteins metabolism
- Abstract
The Kluyveromyces lactis killer toxin zymocin insensitive 11 (KTI11) gene from Saccharomyces cerevisiae is allelic with the diphthamide synthesis 3 (DPH3) locus. Here, we present evidence that the KTI11 gene product is a versatile partner of proteins and operates in multiple biological processes. Notably, Kti11 immune precipitates contain Elp2 and Elp5, two subunits of the Elongator complex which is involved in transcription, tRNA modification and zymocin toxicity. KTI11 deletion phenocopies Elongator-minus cells and causes antisuppression of nonsense and missense suppressor tRNAs (SUP4, SOE1), zymocin resistance and protection against the tRNase attack of zymocin. In addition and unlike Elongator mutants, kti11 mutants resist diphtheria toxin (DT), protect against ADP-ribosylation of eukaryotic translation elongation factor 2 (eEF2) by DT and induce resistance against sordarin, an eEF2 poisoning antifungal. The latter phenotype applies to all diphthamide mutants (dph1-dph5) tested and Kti11/Dph3 physically interacts with diphthamide synthesis factors Dph1 and Dph2, presumably as part of a trimeric complex. Moreover, we present a separation of function mutation in KTI11, kti11-1, which dissociates zymocin resistance from DT sensitivity. It encodes a C-terminal Kti11 truncation that almost entirely abolishes Elongator interaction without affecting association with Kti13, another Kti11 partner protein.
- Published
- 2008
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184. Yeast alpha-tubulin suppressor Ats1/Kti13 relates to the Elongator complex and interacts with Elongator partner protein Kti11.
- Author
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Zabel R, Bär C, Mehlgarten C, and Schaffrath R
- Subjects
- Biological Transport, Cell Cycle Proteins genetics, Cell Cycle Proteins metabolism, Cytoplasm chemistry, Cytoplasm genetics, Cytoplasm metabolism, Gene Expression Regulation, Fungal, Killer Factors, Yeast, Kluyveromyces metabolism, Mutation, Mycotoxins pharmacology, Nuclear Proteins genetics, Nuclear Proteins metabolism, Nucleosome Assembly Protein 1, Peptide Elongation Factors genetics, Protein Binding, RNA, Transfer metabolism, Repressor Proteins analysis, Repressor Proteins genetics, Saccharomyces cerevisiae chemistry, Saccharomyces cerevisiae drug effects, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae Proteins analysis, Saccharomyces cerevisiae Proteins genetics, Suppression, Genetic, Peptide Elongation Factors metabolism, Repressor Proteins metabolism, Saccharomyces cerevisiae metabolism, Saccharomyces cerevisiae Proteins metabolism
- Abstract
The alpha-tubulin suppressor 1 (ATS1) gene and the killer toxin-insensitive 13 (KTI13) locus from Saccharomyces cerevisiae are allelic. The Ats1/Kti13 gene product interacts with the cell polarity factor Nap1 and promotes growth inhibition of S. cerevisiae by zymocin, a tRNAse toxin complex from Kluyveromyces lactis. Kti13 removal causes zymocin resistance, a trait that is typical of defects in the Elongator complex. Here, we show that Kti13 co-purifies with the Elongator partner protein Kti11 and that the Kti11 interaction, not the Nap1 partnership, requires the C-terminus of Kti13. Moreover, Kti13 functionally relates to roles of the Elongator complex in tRNA wobble uridine modification, tRNA suppression of nonsense (SUP4) and missense (SOE1) mutations and tRNA restriction by zymocin. Also, inactivation of Kti13 or Elongator rescues the thermosensitive growth defect of secretory mutants (sec2-59(ts), sec12-4(ts)), suggesting that Kti13 and Elongator affect secretion processes that depend on the GTP exchange factors Sec2 and Sec12 respectively. Distinct from tandem deletions in KTI13 and Elongator genes, a kti13Delta kti11Delta double deletion induces synthetic sickness or lethality. In sum, our data suggest that Kti13 and Kti11 support Elongator functions and that they both share Elongator-independent role(s) that are important for cell viability.
- Published
- 2008
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185. tRNAGlu wobble uridine methylation by Trm9 identifies Elongator's key role for zymocin-induced cell death in yeast.
- Author
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Jablonowski D, Zink S, Mehlgarten C, Daum G, and Schaffrath R
- Subjects
- Base Sequence, DNA Primers, Epistasis, Genetic, Killer Factors, Yeast, Methylation, Reverse Transcriptase Polymerase Chain Reaction, Saccharomyces cerevisiae cytology, Cell Death drug effects, Mycotoxins pharmacology, RNA, Transfer, Glu metabolism, Saccharomyces cerevisiae drug effects, Saccharomyces cerevisiae Proteins metabolism, Uridine metabolism, tRNA Methyltransferases metabolism
- Abstract
Zymocin-induced cell death in Saccharomyces cerevisiae requires the toxin-target (TOT) effector Elongator, a protein complex with functions in transcription, exocytosis and tRNA modification. In line with the latter, trm9Delta cells lacking a tRNA methylase specific for wobble uridine (U(34)) residues survive zymocin and in excess, the Trm9 substrate tRNA(Glu) copies zymocin protection of Elongator mutants. Phenotypes typical of a tot3/elp3Delta Elongator mutant are absent from trm9Delta cells but copied in a tot3Deltatrm9Delta double mutant suggesting that Elongator acts upstream of Trm9. Consistent with Elongator-dependent tRNA modification being more important to mRNA decoding than Trm9, SUP4 and SOE1TRNA suppressors are highly sensitive to loss of Elongator and tRNA U(34) hypomodification. As Trm9 overexpression counteracts the effect of high-copy tRNA(Glu), zymocin suppression by high-copy tRNA(Glu) may reflect tRNA hypomethylation of trm9Delta cells. Thus, Trm9 methylation may enable recognition of tRNA by zymocin, a notion supported by a dramatic reduction of tRNA(Glu) levels in zymocin-treated cells and by cytotoxic zymocin residues conserved between bacterial nucleases and a tRNA modifying GTPase. In sum, Trm9 is a bona fideTOT pathway component whose methylation may be hijacked by zymocin to target tRNA function and eventually, mRNA translation.
- Published
- 2006
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186. Elongator's toxin-target (TOT) function is nuclear localization sequence dependent and suppressed by post-translational modification.
- Author
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Fichtner L, Jablonowski D, Schierhorn A, Kitamoto HK, Stark MJ, and Schaffrath R
- Subjects
- Active Transport, Cell Nucleus, Fungal Proteins genetics, Fungal Proteins metabolism, G1 Phase, Gene Deletion, Genes, Fungal, Genes, Reporter, Green Fluorescent Proteins, Histone Acetyltransferases, Karyopherins metabolism, Killer Factors, Yeast, Kluyveromyces metabolism, Luminescent Proteins genetics, Luminescent Proteins metabolism, Nuclear Proteins metabolism, Peptide Elongation Factors metabolism, Protein Interaction Mapping, Protein Processing, Post-Translational, RNA Polymerase II metabolism, Repressor Proteins genetics, Repressor Proteins metabolism, Ribonucleoproteins, Small Nucleolar metabolism, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae growth & development, Saccharomyces cerevisiae Proteins genetics, Saccharomyces cerevisiae Proteins metabolism, Mycotoxins metabolism, Nuclear Localization Signals metabolism, Saccharomyces cerevisiae metabolism
- Abstract
The toxin target (TOT) function of the Saccharomyces cerevisiae Elongator complex enables Kluyveromyces lactis zymocin to induce a G1 cell cycle arrest. Loss of a ubiquitin-related system (URM1-UBA4 ) and KTI11 enhances post-translational modification/proteolysis of Elongator subunit Tot1p (Elp1p) and abrogates its TOT function. Using TAP tagging, Kti11p contacts Elongator and translational proteins (Rps7Ap, Rps19Ap Eft2p, Yil103wp, Dph2p). Loss of YIL103w and DPH2 (involved in diphtheria toxicity) suppresses zymocicity implying that both toxins overlap in a manner mediated by Kti11p. Among the pool that co-fractionates with RNA polymerase II (pol II) and nucleolin, Nop1p, unmodified Tot1p dominates. Thus, modification/proteolysis may affect association of Elongator with pol II or its localization. Consistently, an Elongator-nuclear localization sequence (NLS) targets green fluorescent protein (GFP) to the nucleus, and its truncation yields TOT deficiency. Similarly, KAP120 deletion rescues cells from zymocin, suggesting that Elongator's TOT function requires NLS- and karyopherin-dependent nuclear import.
- Published
- 2003
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187. Subunit communications crucial for the functional integrity of the yeast RNA polymerase II elongator (gamma-toxin target (TOT)) complex.
- Author
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Frohloff F, Jablonowski D, Fichtner L, and Schaffrath R
- Subjects
- Acetyltransferases chemistry, Histone Acetyltransferases, I-kappa B Kinase, Killer Factors, Yeast, Protein Serine-Threonine Kinases metabolism, Protein Subunits, Saccharomyces cerevisiae Proteins chemistry, Acetyltransferases metabolism, Mycotoxins metabolism, RNA Polymerase II metabolism, Saccharomyces cerevisiae Proteins metabolism
- Abstract
In response to the Kluyveromyces lactis zymocin, the gamma-toxin target (TOT) function of the Saccharomyces cerevisiae RNA polymerase II (pol II) Elongator complex prevents sensitive strains from cell cycle progression. Studying Elongator subunit communications, Tot1p (Elp1p), the yeast homologue of human IKK-associated protein, was found to be essentially involved in maintaining the structural integrity of Elongator. Thus, the ability of Tot2p (Elp2p) to interact with the HAT subunit Tot3p (Elp3p) of Elongator and with subunit Tot5p (Elp5p) is dependent on Tot1p (Elp1p). Also, the association of core-Elongator (Tot1-3p/Elp1-3p) with HAP (Elp4-6p/Tot5-7p), the second three-subunit subcomplex of Elongator, was found to be sensitive to loss of TOT1 (ELP1) gene function. Structural integrity of the HAP complex itself requires the ELP4/TOT7, ELP5/TOT5, and ELP6/TOT6 genes, and elp6Delta/tot6Delta as well as elp4Delta/tot7Delta cells can no longer promote interaction between Tot5p (Elp5p) and Tot2p (Elp2p). The association between Elongator and Tot4p (Kti12p), a factor that may modulate the TOT activity of Elongator, requires Tot1-3p (Elp1-3p) and Tot5p (Elp5p), indicating that this contact requires a preassembled holo-Elongator complex. Tot4p also binds pol II hyperphosphorylated at its C-terminal domain Ser(5) raising the possibility that Tot4p bridges the contact between Elongator and pol II.
- Published
- 2003
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188. Protein interactions within Saccharomyces cerevisiae Elongator, a complex essential for Kluyveromyces lactis zymocicity.
- Author
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Fichtner L, Frohloff F, Jablonowski D, Stark MJ, and Schaffrath R
- Subjects
- Adaptor Proteins, Signal Transducing, Killer Factors, Yeast, Mutagenesis, Insertional, Mutation, Phenotype, RNA Polymerase II genetics, RNA Polymerase II metabolism, RNA, Messenger metabolism, Saccharomyces cerevisiae Proteins biosynthesis, Saccharomyces cerevisiae Proteins genetics, Transcription, Genetic, Two-Hybrid System Techniques, Kluyveromyces metabolism, Mycotoxins metabolism, Saccharomyces cerevisiae metabolism, Saccharomyces cerevisiae Proteins metabolism, Saccharomyces cerevisiae Proteins physiology
- Abstract
mTn3-tagging identified Kluyveromyces lactis zymocin target genes from Saccharomyces cerevisiae as TOT1-3/ELP1-3 coding for the RNA polymerase II (pol II) Elongator histone acetyltransferase (HAT) complex. tot phenotypes resulting from mTn3 tagging were similar to totDelta null alleles, suggesting loss of Elongator's integrity. Consistently, the Tot1-3/Elp1-3 proteins expressed from the mTn3-tagged genes were all predicted to be C-terminally truncated, lacking approximately 80% of Tot1p, five WD40 Tot2p repeats and two HAT motifs of Tot3p. Besides its role as a HAT, Tot3p assists subunit communication within Elongator by mediating Tot2-Tot4, Tot2-Tot5, Tot2-Tot1 and Tot4-Tot5 protein-protein interactions. TOT1 and TOT2 are essential for Tot4-Tot2 and Tot4-Tot3 interactions respectively. The latter was lost with a C-terminal Tot2p truncation; the former was affected by progressively truncating TOT1. Despite being dispensable for Tot4-Tot2 interaction, the extreme C-terminus of Tot1p may play a role in TOT/Elongator function, as its truncation confers zymocin resistance. Tot4p/Kti12p, an Elongator-associated factor, also interacted with pol II and could be immunoprecipitated while being bound to the ADH1 promoter. Two-hybrid analysis showed that Tot4p also interacts with Cdc19p, suggesting that Tot4p plays an additional role in concert with Cdc19p, perhaps co-ordinating cell growth with carbon source metabolism.
- Published
- 2002
- Full Text
- View/download PDF
189. Molecular analysis of KTI12/TOT4, a Saccharomyces cerevisiae gene required for Kluyveromyces lactis zymocin action.
- Author
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Fichtner L, Frohloff F, Bürkner K, Larsen M, Breunig KD, and Schaffrath R
- Subjects
- Adaptor Proteins, Signal Transducing, Amino Acid Sequence, Fungal Proteins metabolism, Gene Dosage, Histone Methyltransferases, Killer Factors, Yeast, Kluyveromyces genetics, Methyltransferases genetics, Methyltransferases metabolism, Molecular Sequence Data, Mycotoxins genetics, Protein Methyltransferases, RNA Polymerase II genetics, RNA Polymerase II metabolism, Sequence Homology, Amino Acid, Fungal Proteins genetics, Histone-Lysine N-Methyltransferase, Kluyveromyces metabolism, Mycotoxins metabolism, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae Proteins
- Abstract
TOT, the putative Kluyveromyces lactis zymocin target complex from Saccharomyces cerevisiae, is encoded by TOT1-7, six loci of which are isoallelic to RNA polymerase II (RNAPII) Elongator genes (ELP1-6). Unlike TOT1-3 (ELP1-3) and TOT5-7 (ELP5, ELP6 and ELP4 respectively), which display zymocin resistance when deleted, TOT4 (KTI12) also renders cells refractory to zymocin when maintained in multicopy or overexpressed from the GAL10 promoter. Elevated TOT4 copy number results in an intermediate tot phenotype, which includes mild sensitivities towards caffeine, Calcofluor white and elevated growth temperature, suggesting that TOT4 influences TOT/Elongator function. Tot4p interacts with Elongator, as shown by co-immunoprecipitation, and cell fractionation studies demonstrate partial co-migration with RNAPII and Elongator. As Elongator subunit interaction is not affected by either deletion of TOT4 or multicopy TOT4, Tot4p may not be a structural Elongator subunit but, rather, may regulate TOT/Elongator in a fashion that requires transient physical contact with TOT/Elongator. Consistent with a regulatory role, the presence of a potential P-loop motif conserved between yeast and human TOT4 homologues suggests capability of ATP or GTP binding and P-loop deletion renders Tot4p biologically inactive.
- Published
- 2002
- Full Text
- View/download PDF
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