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Determinants of eukaryal cell killing by the bacterial ribotoxin PrrC.
- Source :
-
Nucleic acids research [Nucleic Acids Res] 2011 Jan; Vol. 39 (2), pp. 687-700. Date of Electronic Publication: 2010 Sep 19. - Publication Year :
- 2011
-
Abstract
- tRNA damage inflicted by the Escherichia coli anticodon nuclease PrrC (EcoPrrC) underlies an antiviral response to phage T4 infection. PrrC homologs are present in many bacterial proteomes, though their biological activities are uncharted. PrrCs consist of two domains: an N-terminal NTPase module related to the ABC family and a distinctive C-terminal ribonuclease module. In this article, we report that the expression of EcoPrrC in budding yeast is fungicidal, signifying that PrrC is toxic in a eukaryon in the absence of other bacterial or viral proteins. Whereas Streptococcus PrrC is also toxic in yeast, Neisseria and Xanthomonas PrrCs are not. Via analysis of the effects of 118 mutations on EcoPrrC toxicity in yeast, we identified 22 essential residues in the NTPase domain and 11 in the nuclease domain. Overexpressing PrrCs with mutations in the NTPase active site ameliorated the toxicity of wild-type EcoPrrC. Our findings support a model in which EcoPrrC toxicity is contingent on head-to-tail dimerization of the NTPase domains to form two composite NTP phosphohydrolase sites. Comparisons of EcoPrrC activity in a variety of yeast genetic backgrounds, and the rescuing effects of tRNA overexpression, implicate tRNA(Lys(UUU)) as a target of EcoPrrC toxicity in yeast.
- Subjects :
- Alanine genetics
Amino Acid Sequence
Bacterial Proteins metabolism
Bacterial Toxins genetics
Escherichia coli Proteins genetics
Molecular Sequence Data
Mutagenesis
Protein Structure, Tertiary
RNA, Transfer chemistry
RNA, Transfer, Lys metabolism
Ribonucleases genetics
Saccharomyces cerevisiae metabolism
Sequence Homology, Amino Acid
Structure-Activity Relationship
Bacterial Toxins chemistry
Bacterial Toxins metabolism
Escherichia coli Proteins chemistry
Escherichia coli Proteins metabolism
Ribonucleases chemistry
Ribonucleases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1362-4962
- Volume :
- 39
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Nucleic acids research
- Publication Type :
- Academic Journal
- Accession number :
- 20855293
- Full Text :
- https://doi.org/10.1093/nar/gkq831