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Plain concrete’s major two drawbacks are its low tensile strength and high carbon footprint. Joint adding of fibers and recycled/waste materials in concrete might assist to resolve these problems. In the present study, a novel technique is planned to improve the recycled aggregate concrete (RAC) mechanical behavior and durability performance by joint incorporation of silica fume (SF) and nylon fibers (NF). In this research paper, different properties of concrete samples are examined for example flexural strength, compressive strength, split tensile strength, penetration of chloride ions, acid resistance, and water absorption. It was noted that adding nylon fibers as individual components enhances the recycled aggregate concrete mechanical characteristics and resistance to acid exposure. The inclusion of nylon fibers improved the behavior of the recycled aggregate concrete; however, it also increased the chloride penetration and water absorption by only 18% and 8% respectively. Up to 26% of mechanical strength of concrete was improved when silica fume was added in comparison to reference concrete, silica fume also assisted in controlling the loss of durability because of adding recycled aggregate concrete and nylon fibers. Silica fume improved the bond between binder matrix and nylon fibers. The study revealed that the combination of 50% RCA, 0.5% nylon fibers and 20% silica fume are optimum for the joint incorporation into concrete that can assist in developing sustainable, durable, and ductile recycled aggregate fiber reinforced concrete.

The current practice of concrete is thought to be unsuitable because it consumes large amounts of cement, sand, and aggregate, which causes depletion of natural resources. In this study, a step towards sustainable concrete was made by utilizing recycled concrete aggregate (RCA) as a coarse aggregate. However, researchers show that RCA causes a decrease in the performance of concrete due to porous nature. In this study, waste glass (WG) was used as a filler material that filled the voids between RCA to offset its negative impact on concrete performance. The substitution ratio of WG was 10, 20, or 30% by weight of cement, and RCA was 20, 40, and 60% by weight of coarse aggregate. The slump cone test was used to assess the fresh property, while compressive, split tensile, and punching strength were used to assess the mechanical performance. Test results indicated that the workability of concrete decreased with substitution of WG and RCA while mechanical performance improved up to a certain limit and then decreased due to lack of workability. Furthermore, a statical tool response surface methodology was used to predict various strength properties and optimization of RCA and WG.

The aquaculture sector has experienced rapid and important growth with the subsequent increase of feeding and nutritional issues for sustaining this activity, mainly related to the use of high quality, safe and environmentally friendly feed ingredients. The use of additives in aquafeeds has proven to be a suitable option to improve different productive indicators in farmed fish. In the present study, the effect of adding the GHRP-6 peptide, a ghrelin analog, to a commercial diet of gilthead sea bream (Sparus aurata) was studied at two proportions (100 or 500 μg/kg of feed). Both experimental diets show an increase in growth performance, as well as in feed efficiency after 97 days of experiment. The lower inclusion of GHRP-6 (100 μg/kg) results in a better aerobic metabolism, while the higher inclusion significantly increased plasma GH levels in agreement with the GH secretagogue effects of ghrelin. Similar growth outcome and differences between GHRP-6 levels in aerobic metabolism and GH stimulation suggest that improvements in culture performance by this peptide may occur through different mechanisms. Taken together, this compound can be considered as a viable dietary supplement for increasing production efficiency of sea bream aquaculture, although a better understanding of its dose-specific effects is still required.

Femtosecond laser micromachining is becoming an established fabrication technique for transparent material processing in three dimensions [...]

Abstract:: Primary Hyperoxaluria Type I (PH1) is a rare autosomal recessive metabolic disorder characterized by defects in enzymes involved in glyoxylate metabolism. PH1 is a life-threatening disease caused by the absence, deficiency or mistargeting of the hepatic alanine-glyoxylate aminotransferase (AGT) enzyme. A human induced pluripotent stem cell (iPSC) line was generated from dermal fibroblasts of a PH1 patient being compound heterozygous for the most common mutation c.508G>A (G170R), a mistargeting mutation, and c.364C>T (R122*), a previously reported nonsense mutation in AGTX. This iPSC line offers a useful resource to study the disease pathophysiology and a cell-based model for drug development.

Recycled aggregate is a good option to be used in concrete production as a coarse aggregate that results in environmental benefits as well as sustainable development. However, recycled aggregate causes a reduction in the mechanical and durability performance of concrete. On the other hand, the removal of industrial waste would be considerably decreased if it could be incorporated into concrete production. One of these possibilities is the substitution of the cement by slag, which enhances the concrete poor properties of recycled aggregate concrete as well as provides a decrease in cement consumption, reducing carbon dioxide production, while resolving a waste management challenge. Furthermore, steel fiber was also added to enhance the tensile capacity of recycled aggregate concrete. The main goal of this study was to investigate the characteristics of concrete using ground granulated blast-furnace slag (GGBS) as a binding material on recycled aggregate fibers reinforced concrete (RAFRC). Mechanical performance was assessed through compressive strength and split tensile strength, while durability aspects were studied through water absorption, acid resistance, and dry shrinkage. The results detected from the different experiments depict that, at an optimum dose (40% RCA, 20%GGBS, and 2.0%), compressive and split tensile strength were 39% and 120% more than the reference concrete, respectively. Furthermore, acid resistance at the optimum dose was 36% more than the reference concrete. Furthermore, decreased water absorption and dry shrinkage cracks were observed with the substitution of GGBS into RAFRC.

The linear economy paradigm in place to date has to be seriously challenged to give way to a new school of thought known as the circular economy. In this research work, precast kerbs and paving blocks made with recycled concrete (RACC-mixture) bearing 50 wt% mixed recycled aggregate (masonry content of 33%) and an eco-efficient cementitious material as 25 wt% conventional binder replacement were evaluated to assess their intrinsic potential to replace traditional raw materials, in keeping with circular economy criteria. Therefore, precast products were subjected to mechanical strength, durability and microstructure tests and were compared to conventional concrete units (CC-mixture and commercially available precast elements). Although a class demotion was observed for water absorption and some decreases in flexural strength (26%), splitting tensile strength (12.8%) and electrical resistivity (45%) and a lower class water absorption were registered, and the recycled mixture also exhibited a greater performance in terms of compressive strength (6%), a better abrasion resistance classification and a comparable porosity and microstructure, which ensures a good concrete durability. In any case, the results showed that precast pieces were European standard-compliant, thus supporting the viability of the mixed recycled aggregates and eco-efficient cementitious replacement in footways.

Industrial effluents from chromium-based products lead to chromium pollution in the environment. Several technologies have been employed for the removal of chromium (Cr) from the environment, including adsorption, ion-exchange, bioremediation, etc. In this study, we isolated a Cr (VI)-resistant fungus, Purpureocillium lilacinum, from contaminated soil, which could reduce chromium. We also characterized a reductant activity of dichromate found in the cellular fraction of the fungus: optimal pH and temperature, effect of enzymatic inhibitors and enhancers, metal ions, use of electron donors, and initial Cr (VI) and protein concentration. This study also shows possible mechanisms that could be involved in the elimination of this metal. We observed an increase in the reduction of Cr (VI) activity in the presence of NADH followed by that of formate and acetate, as electron donor. This reduction was highly inhibited by EDTA followed by NaN3 and KCN, and this activity showed the highest activity at an optimal pH of 7.0 at 37 °C with a protein concentration of 3.62 µg/mL.

The communication of the NGO in Argentina and Spain involves an excellent creativity which is not reflected in the collective minds doubt to the increasing need of professionalize the communication for social causes and elaborate a communication plan. The use they make of new technologies in communication is not appropriate. Having a web page designed by professionals is not enough, it is necessary to establishing communications within media and social networks helps to join forces to face the lack of budgets since it reduces costs and improves the target segmentation. [ABSTRACT FROM AUTHOR]

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One of the prime objectives of this review is to understand the role of design parameters on the mechanical properties (Compressive and split tensile strength) of Self-Compacting Concrete (SCC) with recycled aggregates (Recycled Coarse Aggregates (RCA) and Recycled Fine Aggregates (RFA)). The design parameters considered for review are Water to Cement (W/C) ratio, Water to Binder (W/B) ratio, Total Aggregates to Cement (TA/C) ratio, Fine Aggregate to Coarse Aggregate (FA/CA) ratio, Water to Solid (W/S) ratio in percentage, superplasticizer (SP) content (kg/cu.m), replacement percentage of RCA, and replacement percentage of RFA. It is observed that with respect to different grades of SCC, designed parameters affect the mechanical properties of SCC with recycled aggregates.

Most concrete studies are concentrated on mechanical properties especially strength properties either directly or indirectly (fresh and durability properties). Hence, the ratio of split tensile strength to compressive strength plays a vital role in defining the concrete properties. In this review, the impact of design parameters on the strength ratio of various grades of Self-Compacting Concrete (SCC) with recycled aggregate is assessed. The design parameters considered for the study are Water to Cement (W/C) ratio, Water to Binder (W/B) ratio, Total Aggregates to Cement (TA/C) ratio, Fine Aggregate to Coarse Aggregate (FA/CA) ratio, Water to Solid (W/S) ratio in percentage, superplasticizer (SP) content (kg/cu.m), replacement percentage of recycled coarse aggregates (RCA), replacement percentage of recycled fine aggregates (RFA), fresh density and loading area of the specimen. It is observed that the strength ratio of SCC with recycled aggregates is affected by design parameters.

Introdução: Na atenção primária à saúde, o Ministério da Saúde recomenda que pacientes hipertensos que estiverem com a pressão arterial descontrolada, mas que estejam cumprindo os tratamentos recomendados, deverão realizar consulta médica mensal para reavaliação, até atingirem a meta pressórica estabelecida. Baseado nisso, o projeto aplicativo aconteceu em uma comunidade com 8000 usuários, onde há uma elevada taxa de hipertensão entre a população, associada à falta de informação, baixa condição socioeconômica e falta de adesão às consultas e ao HiperDiA. A partir desse cenário, foram realizadas ações que implicassem na conscientização e prevenção das complicações da hipertensão e na redução do número de novos hipertensos na área. Objetivo: Melhorar a adesão ao tratamento e acompanhamento dos hipertensos cadastrados na USF Francisco Marcelo Dias, em Paulista-PE. Métodos: Estudo descritivo do tipo relato de projeto aplicativo. Utilizou-se a metodologia da problematização, baseado no Arco de Maguerez para identificação do problema base da área, teorização, criação de hipóteses de solução e um plano de ação. Resultado: As ações prezaram a prevenção e conscientização para uma melhor qualidade de vida, evitando assim tanto o desenvolvimento da hipertensão como também a evolução da doença. Para isso, as atividades realizadas na USF foram aferição da pressão arterial e rodas de conversa sobre a importância da alimentação saudável e o uso contínuo dos medicamentos. Essas ações tiveram um bom impacto ao modo que aumentaram o número de pacientes nas consultas e no HiperDia, aumentaram a quantidade de adeptos ao tratamento, e através do incentivo à mudança nos hábitos de vida, espera-se que a longo prazo haja uma diminuição no número de novos hipertensos na área. Conclusão: A promoção de saúde adequada para os pacientes hipertensos da comunidade, assim como a intervenção para a prevenção e tratamento da hipertensão arterial apresentou implicações clínicas importantes, uma vez que foi capaz aumentar a adesão ao tratamento e a mudança nos hábitos de vida, prevenindo futuros pacientes hipertensos e melhorando a qualidade de vida dos hipertensos já diagnosticados da área.

Introdução: Entre os medicamentos mais prescritos mundialmente encontram-se os inibidores da bomba de prótons (IBP), que apresentam bastante utilidade no tratamento de doenças gástricas e eficácia pela sua baixa toxicidade e bloqueio da secreção gástrica. Apesar da sua utilidade durante o tratamento, o uso prolongado destes medicamentos pode causar sérias complicações e prejuízos para o paciente que faz uso contínuo desses fármacos. Com o intuito de diminuir a dose ou interromper o uso de medicamentos que possam trazer danos, tem-se investido muito na desprescrição de IBP, que deve ser realizada de modo a considerar os benefícios, a finalidade do tratamento, a comodidade, a idade e também a cooperação do paciente. Realizar uma revisão de literatura a respeito dos efeitos negativos Objetivo: e positivos da desprescrição dos inibidores de bomba de prótons. Métodos: Revisão narrativa a respeito da desprescrição do IBP realizada na Biblioteca Virtual de Saúde (BVS) e nos bancos de dados SCIELO, LILACS, usando os descritores: desprescrição, toxicidade, ácido gástrico e esofagite. Foram selecionados 15 artigos publicados nos últimos 12 anos que envolviam os três núcleos temáticos: Ação dos inibidores de bomba de protóns sob a secreção gástrica, complicações pelo uso prolongado de IBP e a importância da desprescrição dos IBPs. Resultados: Sabe-se que vários pacientes acabam automedicando-se ou dando continuidade a tratamentos anteriores sem o conhecimento médico; além disso, o uso de muitos fármacos juntos sem uma análise prévia para a patologia pode ocasionar várias complicações. Desse modo, a desprescrição desses medicamentos, especialmente dos IBPs, é uma das maneiras de impedir efeitos indesejados,doenças no pacientee até mesmo complicações de doenças pré-existentes. A importância da desprescrição é evitar que IBPs sejam prescritos por tempo indeterminado e também garantir a segurança do paciente evitando sua exposição a riscos inerentes de reações adversas, erros de medicação, interações medicamentosas e internações em decorrência de complicações para o mesmo. Conclusão: Nesse sentido, embora sejam potentes para as doenças gástricas, os IBPs são desnecessários para algumas doenças digestivas, daí a importância de uma equipe multidisciplinar para tratar o paciente e reduzir a prescrição destes IBPs. Concluímos que para realizar a desprescrição é preciso, então, escolher o tratamento que tenha uma abordagem geral e que traga menos prejuízo à saúde e à vida do paciente.

Synaptophysins 1 and 2 and synaptogyrins 1 and 3 constitute a major family of synaptic vesicle membrane proteins. Unlike other widely expressed synaptic vesicle proteins such as vSNAREs and synaptotagmins, the primary function has not been resolved. Here, we report robust elevation in the probability of release of readily releasable vesicles with both high and low release probabilities at a variety of synapse types from knockout mice missing all four family members. Neither the number of readily releasable vesicles, nor the timing of recruitment to the readily releasable pool was affected. The results suggest that family members serve as negative regulators of neurotransmission, acting directly at the level of exocytosis to dampen connection strength selectively when presynaptic action potentials fire at low frequency. The widespread expression suggests that chemical synapses may play a frequency filtering role in biological computation that is more elemental than presently envisioned.Editorial note: This article has been through an editorial process in which the authors decide how to respond to the issues raised during peer review. The Reviewing Editor's assessment is that all the issues have been addressed (see decision letter).

In this study, the SWAT (Soil Water Assessment Tool) hydrological model is implemented to determine the effect of climate change on various hydrological components in two basins located in the foothills of the Andes: the Quino and Muco river basins. The water cycle is analyzed by comparing the model results to climatic data observed in the past (1982–2016) to understand its trend behaviors. Then, the variations and geographical distribution of the components of the hydrological cycle were analyzed using the Representative Concentration Pathway (RCP)8.5 climate scenario to model two periods considering the immediate future (2020–2049) and intermediate future (2050–2079). In this way, in the study area, it is predicted that yearly average temperatures will increase up to 1.7 °C and that annual average precipitation will decrease up to 210 mm for the intermediate future. Obtained results show that the analyzed parameters presented the same trend behavior for both periods of time; however, a greater impact can be expected in the intermediate future. According to the spatial distribution, the impact worsens for all the parameters as the elevation increases in both basins. The model depicted that yearly average evapotranspiration would increase around 5.26% and 5.81% for Quino and Muco basins, respectively, due to the large increase in temperature. This may cause, when combined with the precipitation lessening, a decrease around 9.52% and 9.73% of percolation, 2.38% and 1.76% of surface flow, and 7.44% and 8.14% of groundwater for Quino and Muco basins, respectively, with a consequent decrease of the water yield in 5.25% and 4.98% in the aforementioned watersheds, respectively.

Femtosecond laser micromachining (FLM) of fused silica allows for the realization of three-dimensional embedded optical elements and microchannels with micrometric feature size. The performances of these components are strongly affected by the machined surface quality and residual roughness. The polishing of 3D buried structures in glass was demonstrated using different thermal annealing processes, but precise control of the residual roughness obtained with this technique is still missing. In this work, we investigate how the FLM irradiation parameters affect surface roughness and we characterize the improvement of surface quality after thermal annealing. As a result, we achieved a strong roughness reduction, from an average value of 49 nm down to 19 nm. As a proof of concept, we studied the imaging performances of embedded mirrors before and after thermal polishing, showing the capacity to preserve a minimum feature size of the reflected image lower than μ5μm. These results allow for us to push forward the capabilities of this enabling fabrication technology, and they can be used as a starting point to improve the performances of more complex optical elements, such as hollow waveguides or micro-lenses.

During the epithelial-to-mesenchymal transition, the intracellular cytoskeleton undergoes severe reorganization which allows epithelial cells to transition into a motile mesenchymal phenotype. Among the different cytoskeletal elements, the intermediate filaments keratin (in epithelial cells) and vimentin (in mesenchymal cells) have been demonstrated to be useful and reliable histological markers. In this study, we assess the potential invasiveness of six human breast carcinoma cell lines and two mouse fibroblasts cells lines through single cell migration assays in confinement. We find that the keratin and vimentin networks behave mechanically the same when cells crawl through narrow channels and that vimentin protein expression does not strongly correlate to single cells invasiveness. Instead, we find that what determines successful migration through confining spaces is the ability of cells to mechanically switch from a substrate-dependent stress fibers based contractility to a substrate-independent cortical contractility, which is not linked to their tumor phenotype.

This article presents an overview of the bibliographic picture of the design parameter’s influence on the mix proportion of self-compacting concrete with recycled aggregate. Design parameters like water-cement ratio, water to paste ratio, and percentage of superplasticizers are considered in this review. Standardization and recent research on the usage of recycled aggregates in self-compacting concrete (SCC) exploit its significance in the construction sector. The usage of recycled aggregate not only resolves the negative impacts on the environment but also prevents the usage of natural resources. Furthermore, it is necessary to understand the recycled aggregate property’s role in a mixed design and SCC properties. Design parameters are not only influenced by a mix design but also play a key role in SCC’s fresh properties. Hence, in this overview, properties of SCC ingredients, calculation of design parameters in mix design, the effect of design parameters on fresh concrete properties, and the evolution of fresh concrete properties are studied.

Age-inappropriate expression of juvenile NMDA receptors (NMDARs) containing GluN3A subunits has been linked to synapse loss and death of spiny projection neurons of the striatum (SPNs) in Huntington's disease (HD). Here we show that suppressing GluN3A expression prevents a multivariate synaptic transmission phenotype that precedes morphological signs at early prodromal stages. We start by confirming that afferent fiber stimulation elicits larger synaptic responses mediated by both AMPA receptors and NMDARs in SPNs in the YAC128 mouse model of HD. We then show that the enhancement mediated by both is fully prevented by suppressing GluN3A expression. Strong fiber-stimulation unexpectedly elicited robust NMDAR-mediated electrogenic events (termed “upstates” or “NMDA spikes”), and the effective threshold for induction was more than 2-fold lower in YAC128 SPNs because of the enhanced synaptic transmission. The threshold could be restored to control levels by suppressing GluN3A expression or by applying the weak NMDAR blocker memantine. However, the threshold was not affected by preventing glutamate spillover from synaptic clefts. Instead, long-lasting NMDAR responses interpreted previously as activation of extrasynaptic receptors by spilled-over glutamate were caused by NMDA spikes occurring in voltage clamp mode as escape potentials. Together, the results implicate GluN3A reactivation in a broad spectrum of early-stage synaptic transmission deficits in YAC128 mice; question the current concept that NMDAR mislocalization is the pathological trigger in HD; and introduce NMDA spikes as a new candidate mechanism for coupling NMDARs to neurodegeneration.

Background: Growth hormone secretagogues (GHS), among other factors, regulate the release of GH. The biological activity of the secretagogue peptide A233 as a promoter of growth and innate immunity in teleost fish has previously been demonstrated, but its role in the immune system of mammals is not well understood. Methods: The effect of the peptide was investigated in J774A.2 macrophage cells using a comparative proteomics approach after 6 and 12 h of peptide stimulation. Results: The functional analysis of differentially modulated proteins showed that A233 peptide treatment appears to promote activation and ROS-dependent cytotoxic functions in macrophages and enhanced expression of antiviral protein complexes such as MAVS. In accordance with this hypothesis, we found that A233 treatment enhanced superoxide anion production and the IFN-γ level in J774A.2 cells and mouse splenocytes, respectively, and reduced viral load in a dengue virus mouse model of infection. Conclusions: The growth hormone secretagogue A233 peptide promotes activation of ROS-dependent cytotoxic functions and exerts immunomodulatory effects that enable an antiviral state in a dengue virus mouse model. General Significance: The increase of IFN-γ level and the differential modulation of antiviral proteins by the A233 peptide suggest that the molecule could activate an innate immune response with a possible further impact in the treatment of acute and chronic diseases.

Lactic acid bacteria (LAB) dominate human vaginal microbiota and inhibit pathogen proliferation. In other mammals, LAB do not dominate vaginal microbiota, however shifts of dominant microorganisms occur during ovarian cycle. The study objectives were to characterize equine vaginal microbiota in mares by culture-dependent and independent methods and to describe its variation in estrus and diestrus. Vaginal swabs from 8 healthy adult Arabian mares were obtained in estrus and diestrus. For culture-dependent processing, bacteria were isolated on Columbia blood agar (BA) and Man Rogosa Sharpe (MRS) agar. LAB comprised only 2% of total bacterial isolates and were not related to ovarian phases. For culture-independent processing, V3/V4 variable regions of the 16S ribosomal RNA gene were amplified and sequenced using Illumina Miseq. The diversity and composition of the vaginal microbiota did not change during the estrous cycle. Core equine vaginal microbiome consisted of Firmicutes, Bacteroidetes, Proteobacteria and Actinobacteria at the phylum level. At the genus level it was defined by Porphyromonas, Campylobacter, Arcanobacterium, Corynebacterium, Streptococcus, Fusobacterium, uncultured Kiritimatiaellae and Akkermansia. Lactobacillus comprised only 0.18% of the taxonomic composition in estrus and 0.37% in diestrus. No differences in the relative abundance of the most abundant phylum or genera were observed between estrus and diestrus samples.

Sheep estrous synchronization is mainly based on progestagen-impregnated sponges which could cause vaginitis. Several species of Lactobacillus used as probiotics are commonly used in the treatment or prevention of urogenital infections in humans. However, no studies have been performed to analyze the potential use of probiotics to prevent urogenital infections in sheep. A randomized controlled clinical trial was conducted with 21 one-year-old ewes to develop a model of probiotic infusion in vaginal sponges in order to study their influence in ewe’s vaginal microbiota, general health status, fertility and prolificity. Synchronization of estrus was based on intravaginal sponges for 14 days. Bacterial communities (Enterobacteriaceae and lactic acid bacteria) were highly fluctuating over time and between animals. The safety of probiotic infusion (mix of Lactobacillus spp. 60% L. crispatus, 20% L. brevis and 20% L. gasseri) in the vagina of healthy ewes was firstly confirmed. Neutrophils were observed in 80% (8/10) of the control ewes compared to 36% (4/11) of the ewes in the probiotic group 2 days after sponge removal (p = 0.056). Fertility in the control and probiotic groups was 60% (6/10) and 91% (10/11), respectively p = 0.097. These results suggest that Lactobacillus spp. infusion in the ewe’s vagina does not affect general health status or fertility.

We report on the application of femtosecond laser micromachining to the fabrication of complex glass microdevices, for high-order harmonic generation in gas. The three-dimensional capabilities and extreme flexibility of femtosecond laser micromachining allow us to achieve accurate control of gas density inside the micrometer interaction channel. This device gives a considerable increase in harmonics’ generation efficiency if compared with traditional harmonic generation in gas jets. We propose different chip geometries that allow the control of the gas density and driving field intensity inside the interaction channel to achieve quasi phase-matching conditions in the harmonic generation process. We believe that these glass micro-devices will pave the way to future downscaling of high-order harmonic generation beamlines.

The use of construction and demolition wastes (C&DW) is a trending future option for the sustainability of construction. In this context, a number of works deal with the use of recycled concrete aggregates to produce concrete for structural and non-structural purposes. Nowadays, an important number of C&DW management plants in the European Union (EU) and other countries have developed robust protocols to obtain high-quality coarse recycled aggregates that comply with different European standards in order to be used to produce new concrete. The development of self-compacting concrete (SCC) is another way to boost the sustainability of construction, due to the important reduction of energy employed. Using recycled aggregates is a relatively recent scientific area, however, studies on this material in the manufacture of self-compacting concrete have proven the feasibility thereof for conventional structural elements as well as high-performance and complex structural elements, densely reinforced structures, difficult-to-access formwork and difficult-to-vibrate elements. This paper presents an original study on the use of coarse recycled concrete aggregate (CRA) to obtain self-compacting concrete. Concrete with substitution ratios of 20%, 50% and 100% are compared with a control concrete. The purpose of this comparison is to check the influence of CRA on fresh SCC as well as its physical and mechanical properties. The parameters studied are material characterization, self-compactability, compressive strength, and tensile and flexural strength of the resulting concrete. The results conclude that it is feasible to use CRA for SCC production with minimal losses in the characteristics.

Several impacts over ecosystem services have been produced by land use/cover changes, placing it as one of the main factors driving global environmental change. In the present study, the SWAT model was used to assess the effect of land use/cover changes on the hydrology response in the Andalien river basin from the south-central zone of Chile. Three land use/cover scenarios (LU_1986, LU_2001, and LU_2011) were compared over a period of 30 years (1984−2013) to remove the effect of climate variability on hydrology. The results show a significant decrease in total annual flows among the three LU scenarios. The greater differences in the annual flows of 25.05 m3/s were observed between LU_1986 and LU_2011 scenarios. The hydrological cycle dynamics in the basin show an increasing trend of evapotranspiration and surface flows with a significant decrease in percolation and lateral flow on a monthly and seasonal scale. This behavior can be explained by the increasing percentage of the basin area covered by exotic plantations, from 35.22% to 63.93% during the period. The evidence of these changes and the evaluation of their effects are particularly relevant for the long-term sustainable management of water resources.

Introdução: O médico de família (MF) é com frequência o primeiro contacto do utente com os cuidados de saúde, lidando com a doença numa fase precoce e cujos sintomas são ainda inespecíficos. Com este caso pretende-se promover a reflexão sobre o papel do MF nas várias etapas do processo diagnóstico, bem como as características da medicina geral e familiar e dos cuidados de saúde primários que o facilitam. Descrição do caso: Mulher de 48 anos, pertencente a uma família reconstruída e índice socioeconómico III de Graffar. Tem antecedentes pessoais de síndroma depressivo (medicada com fluoxetina), encontrando-se em climatério. Recorreu à consulta por náuseas e enfartamento com dez dias de evolução, episódio de vómito isolado na véspera de Natal e prurido generalizado. Objetivamente apresentava dor discreta à palpação epigástrica. Foi medicada sintomaticamente e pediu-se avaliação analítica e ecografia abdominal. Regressou três dias depois por icterícia das escleróticas e acolia, mantendo náuseas. Negava colúria ou febre. Os exames complementares de diagnóstico vieram a revelar analiticamente uma hiperbilirrubinemia conjugada; ecograficamente apresentava marcada dilatação da via biliar principal, imagem nodular no corpo/cauda pancreáticos e múltiplos conglomerados adenopáticos adjacentes aos grandes vasos. Não apresentava sintomas consumptivos evidentes. Pediu-se tomografia computorizada abdominal que revelou conglomerado adenopático envolvendo a cabeça do pâncreas, traduzindo provável doença linfoproliferativa. Foi referenciada com caráter urgente à consulta de hemato-oncologia onde se confirmou o diagnóstico: linfoma não-Hodgkin B difuso de grandes células. Cumpriu oito ciclos de quimioterapia e atualmente encontra-se em remissão. Comentário: Apesar de a tomada de decisão do MF dever basear-se no padrão de morbilidade da comunidade e no contexto sociocultural do doente, não deve ser desvalorizada a diversidade de hipóteses de diagnóstico que poderão resultar da evolução natural da doença. O MF deve suportar-se das vantagens da continuidade e da acessibilidade aos cuidados para este processo, potenciando assim o seu papel diagnóstico.

ABSTRACT Mucosa-associated lymphoid tissue (MALT) lymphoma is an extranodal lymphoma arising in a number of epithelial tissues, including stomach, salivary gland and the lung. Pulmonary MALT lymphoma originates in the parenchyma and/or bronchi and is a rare disease, representing 0.5-1% of primary pulmonary malignancies. Although the time to diagnosis can be delayed because most patients are asymptomatic and investigations are usually driven by the accidental finding of abnormal lung imaging studies, it has an indolent course and a good prognosis. Here we report the case of an asymptomatic female patient with a nodular lung opacity that was found to persist five months after an episode of community-acquired pneumonia. A final diagnosis of pulmonary MALT lymphoma was obtained after an extensive diagnostic work-up. RESUMO: O linfoma do tecido linfóide associado à mucosa (MALT) é um linfoma extranodal que surge em vários tecidos epiteliais, incluindo o estômago, as glândulas salivares e o pulmão. O linfoma MALT pulmonar tem origem no parênquima e/ou brônquios e é raro, representando 0.5-1% dos tumors primários do pulmão. Pode haver um atraso no tempo até ao diagnóstico porque a maioria dos pacientes é assintomática, sendo as investigações geralmente conduzidas pelo achado acidental de exames de imagem pulmonares alterados. Este linfoma tem um curso indolente e um bom prognóstico. Aqui expomos o caso de uma paciente assintomática com uma opacidade nodular que persistiu 5 meses após um episódio de pneumonia adquirida na comunidade. O diagnóstico final de linfoma MALT pulmonar foi obtido após uma investigação diagnóstica extensa.

Faecalibacterium prausnitzii is a commensal bacterium, ubiquitous in the gastrointestinal tracts of animals and humans. This species is a functionally important member of the microbiota and studies suggest it has an impact on the physiology and health of the host. F. prausnitzii is the only identified species in the genus Faecalibacterium, but a recent study clustered strains of this species in two different phylogroups. Here, we propose the existence of distinct species in this genus through the use of comparative genomics. Briefly, we performed analyses of 16S rRNA gene phylogeny, phylogenomics, whole genome Multi-Locus Sequence Typing (wgMLST), Average Nucleotide Identity (ANI), gene synteny, and pangenome to better elucidate the phylogenetic relationships among strains of Faecalibacterium. For this, we used 12 newly sequenced, assembled, and curated genomes of F. prausnitzii, which were isolated from feces of healthy volunteers from France and Australia, and combined these with published data from 5 strains downloaded from public databases. The phylogenetic analysis of the 16S rRNA sequences, together with the wgMLST profiles and a phylogenomic tree based on comparisons of genome similarity, all supported the clustering of Faecalibacterium strains in different genospecies. Additionally, the global analysis of gene synteny among all strains showed a highly fragmented profile, whereas the intra-cluster analyses revealed larger and more conserved collinear blocks. Finally, ANI analysis substantiated the presence of three distinct clusters—A, B, and C—composed of five, four, and four strains, respectively. The pangenome analysis of each cluster corroborated the classification of these clusters into three distinct species, each containing less variability than that found within the global pangenome of all strains. Here, we propose that comparison of pangenome subsets and their associated α values may be used as an alternative approach, together with ANI, in the in silico classification of new species. Altogether, our results provide evidence not only for the reconsideration of the phylogenetic and genomic relatedness among strains currently assigned to F. prausnitzii, but also the need for lineage (strain-based) differentiation of this taxon to better define how specific members might be associated with positive or negative host interactions.

Farmers experience whole-body vibrations when they drive tractors. Among the various factors that influence the vibrations to which the driver is exposed are terrain roughness, tractor speed, tire type and pressure, rear axle width, and tractor seat height above the ground. In this paper the influence of tractor seat height above the ground on the lateral vibrations to which the tractor driver is exposed is studied by means of a geometrical and an experimental analysis. Both analyses show that: (i) lateral vibrations experienced by a tractor driver increase linearly with tractor-seat height above the ground; (ii) lateral vibrations to which the tractor driver is exposed can equal or exceed vertical vibrations; (iii) in medium-size tractors, a feasible 30 cm reduction in the height of the tractor seat, which represents only 15% of its current height, will reduce the lateral vibrations by around 20%; and (iv) vertical vibrations are scarcely influenced by tractor-seat height above the ground. The results suggest that manufacturers could increase the comfort of tractors by lowering tractor-seat height above the ground, which will reduce lateral vibrations.

The extra-cellular microenvironment has a fundamental role in tumor growth and progression, strongly affecting the migration strategies adopted by single cancer cells during metastatic invasion. In this study, we use a novel microfluidic device to investigate the ability of mesenchymal and epithelial breast tumor cells to fluidize and migrate through narrowing microstructures upon chemoattractant stimulation. We compare the migration behavior of two mesenchymal breast cancer cell lines and one epithelial cell line, and find that the epithelial cells are able to migrate through the narrowest microconstrictions as the more invasive mesenchymal cells. In addition, we demonstrate that migration of epithelial cells through a highly compressive environment can occur in absence of a chemoattractive stimulus, thus evidencing that they are just as prone to react to mechanical cues as invasive cells.

Excitotoxicity due to excessive activation of glutamate receptors is a primary mediator of cell death in acute and chronic neurological disorders, and NMDA-type glutamate receptors (NMDARs) are thought to be involved. NMDARs assemble from heteromeric combinations of GluN1, GluN2 and GluN3 subunits, yielding a variety of receptor subtypes that differ in biophysical properties, signaling, and synaptic targeting. Inclusion of inhibitory GluN3 subunits reduces Ca2+ influx via NMDAR channels and alters their synaptic targeting, thus modifying the two hallmarks of NMDARs that are critical for their roles on neuronal death and survival. Here we evaluated the neuroprotective potential of GluN3A subunits by analyzing the susceptibility to striatal excitotoxic damage of transgenic mice overexpressing GluN3A. We found that mild GluN3A overexpression protected susceptible striatal neurons from lesions induced by the neurotoxin 3-nitropropionic acid (3-NP), an inhibitor of mitochondrial complex II/succinate dehydrogenase. GluN3A-mediated neuroprotection was dose-dependent, and correlated with the levels of transgenic GluN3A expressed by two different mice strains. Neuroprotection was associated with a potent reduction of the activation of calpain, a Ca2+-dependent protease, which was measured as a decrease in 3-NP-induced fodrin and STEP cleavage in GluN3A transgenic mice relative to controls. We further show that transgenic GluN3A subunits incorporate into extrasynaptic compartments in mouse striatum, suggesting that reductions of toxic calpain activation might be linked to inhibition by GluN3A of pathological extrasynaptic NMDAR activity.

El presente artículo analiza con la finalidad de comprender mejor el ausentismo laboral: ¿Por qué no todos los individuos que enferman faltan al trabajo? ¿Qué tanto las ausencias laborales por enfermedad se relacionan realmente a la falta de salud? y ¿Qué tanto se relaciona el nivel de salud del trabajador con sus ausencias por causa diferente a enfermedad general? Asimismo, el artículo analiza en forma cualitativa cómo se mide el ausentismo de un trabajador cuando se trabaja a distancia (teletrabajo)

Femtosecond laser irradiation followed by chemical etching (FLICE) with hydrogen fluoride (HF) is an emerging technique for the fabrication of directly buried, three-dimensional microfluidic channels in silica. The procedure, as described in literature, consists of irradiating a silica slab followed by chemical etching using hydrogen fluoride. With aqueous HF the etching process is diffusion-limited and is self-terminating, leading to maximum microchannel lengths of about 1.5 mm, while the use of low-pressure gaseous HF etchant can quickly produce 3 mm long channels with an aspect ratio (Length/Diameter) higher than 25. By utilizing this methodology the aspect ratio is not constant, but depends on the length of the channel. When the microchannel is short the aspect ratio increases quickly until it reaches a maximum length at around 1400 µm. Thereafter the aspect ratio starts to decrease slowly. In this paper we present a variation of the low-pressure gaseous HF etching method, which is based on the dynamic displacement of the etchant. This method results in a 13% increase in the aspect ratio (L/D = 29) at the expense of a low etching speed (4 µm/min).

Microinjection moulding combined with the use of removable inserts is one of the most promising manufacturing processes for microfluidic devices, such as lab-on-chip, that have the potential to revolutionize the healthcare and diagnosis systems. In this work, we have designed, fabricated and tested a compact and disposable plastic optical stretcher. To produce the mould inserts, two micro manufacturing technologies have been used. Micro electro discharge machining (µEDM) was used to reproduce the inverse of the capillary tube connection characterized by elevated aspect ratio. The high accuracy of femtosecond laser micromachining (FLM) was exploited to manufacture the insert with perfectly aligned microfluidic channels and fibre slots, facilitating the final composition of the optical manipulation device. The optical stretcher operation was tested using microbeads and red blood cells solutions. The prototype presented in this work demonstrates the feasibility of this approach, which should guarantee real mass production of ready-to-use lab-on-chip devices.

Since the pioneering work of Ashkin and coworkers, back in 1970, optical manipulation gained an increasing interest among the scientific community. Indeed, the advantages and the possibilities of this technique are unsubtle, allowing for the manipulation of small particles with a broad spectrum of dimensions (nanometers to micrometers size), with no physical contact and without affecting the sample viability. Thus, optical manipulation rapidly found a large set of applications in different fields, such as cell biology, biophysics, and genetics. Moreover, large benefits followed the combination of optical manipulation and microfluidic channels, adding to optical manipulation the advantages of microfluidics, such as a continuous sample replacement and therefore high throughput and automatic sample processing. In this work, we will discuss the state of the art of these optofluidic devices, where optical manipulation is used in combination with microfluidic devices. We will distinguish on the optical method implemented and three main categories will be presented and explored: (i) a single highly focused beam used to manipulate the sample, (ii) one or more diverging beams imping on the sample, or (iii) evanescent wave based manipulation.

Se analizaron 16 muestras de maíz, 10 de maíz blanco nacional y 6 de maíz criollo blanco para determinar las especies de Fusarium presentes en los granos, en especial aquellas registradas como inductoras de pudriciones de mazorca y grano, principalmente las que producen micotoxinas. Las especies identificadas en orden del número de aislamientos obtenidos fueron: Fusarium oxysporum, F. subglutinans, F. moniliforme, F. graminearum, F. anthophilum, F. poae, F. tricinctum, F. sporotrichioides y F. proliferatum. Con excepción de F. oxysporum, F. tricinctum y F. anthophilum, las demás han sido registradas como inductoras de pudriciones de mazorca, grano y de tallo, y la mayoría son productoras de diversas micotoxinas, algunas de las cuales pueden ocasionar problemas a la salud humana y animal.Sixteen corn samples, 10 of national white corn and 6 of "criollo" white were analyzed to determine the Fusaria species present on the kernels, especially those reported as ear and kernel rot inducers, mainly those mycotoxin producers. The identified species, ordered in relation to the number of obtained isolates were F. oxysporum, F. subglutinans, F. moniliforme, F. graminearum, F. anthophilum, F. poae, F. tricinctum, F. sporotrichioides, and F. proliferatum. The species F. oxysporum, F. tricinctum, and F. anthophilum have not been reported causing ear or kernel rots. All of the others have been reported as ear and kernel rots inducers, as well as stalk rots. Besides, most of these species produce various mycotoxins, many of which are capable of causing health problems to humans and animals.

Alternative splicing is a key mechanism determinant for gene expression in metazoan. During alternative splicing, non-coding sequences are removed to generate different mature messenger RNAs due to a combination of sequence elements and cellular factors that contribute to splicing regulation. A different combination of splicing sites, exonic or intronic sequences, mutually exclusive exons or retained introns could be selected during alternative splicing to generate different mature mRNAs that could in turn produce distinct protein products. Alternative splicing is the main source of protein diversity responsible for 90% of human gene expression, and it has recently become a hallmark for cancer with a full potential as a prognostic and therapeutic tool. Currently, more than 15,000 alternative splicing events have been associated to different aspects of cancer biology, including cell proliferation and invasion, apoptosis resistance and susceptibility to different chemotherapeutic drugs. Here, we present well established and newly discovered splicing events that occur in different cancer-related genes, their modification by several approaches and the current status of key tools developed to target alternative splicing with diagnostic and therapeutic purposes.

Ultrafast laser microfabrication is a very powerful method for producing integrated devices in transparent materials [1].[...]

Flash photolysis of caged compounds is one of the most powerful approaches to investigate the dynamic response of living cells. Monolithically integrated devices suitable for optical uncaging are in great demand since they greatly simplify the experiments and allow their automation. Here we demonstrate the fabrication of an integrated bio-photonic device for the optical release of caged compounds. Such a device is fabricated using femtosecond laser micromachining of a glass substrate. More in detail, femtosecond lasers are used both to cut the substrate in order to create a pit for cell growth and to inscribe optical waveguides for spatially selective uncaging of the compounds present in the culture medium. The operation of this monolithic bio-photonic device is tested using both free and caged fluorescent compounds to probe its capability of multipoint release and optical sensing. Application of this device to the study of neuronal network activity can be envisaged.

A entrada na Universidade para muitos jovens é marcada por uma fase de transição caracterizada por diversas mudanças que interferem em seu desenvolvimento psicossocial, além do profissional. O presente trabalho teve como objetivo a análise dos fatores que influenciaram na adaptação do aluno, egresso do ensino médio, à vida universitária, bem como a identificação das mudanças comportamentais ocorridas em função desse processo de transição acadêmica. O estudo foi realizado através de entrevistas semi-estrutadas com 8 estudantes pertencentes a diferentes cursos. Os dados foram analisados através da análise de conteúdo e categorizados em dois segmentos: Fatores que Influenciaram na Adaptação e Mudanças Comportamentais. Os resultados indicaram o destaque de sete categorias para o primeiro bloco – Infraestrutura; Informação sobre a Universidade e o curso; Fatores Externo à Universidade; Aspectos Organizacionais; Material de Estudo; Vínculos e Professores – e quatro categorias para o segundo bloco - Aspectos pessoais; Organização pessoal para o estudo; Tempo e Sintomas. O estudo contribui para pontuar as dificuldades encontradas pelos alunos ingressantes no contexto da universidade e proporcionou melhor compreensão sobre a transição acadêmica.

ABSTRACT Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified on the basis of human clinical data. The mechanisms underlying its beneficial effects are still unknown. Gnotobiotic mice harboring F. prausnitzii (A2-165) and Escherichia coli (K-12 JM105) were subjected to 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute colitis. The inflammatory colitis scores and a gas chromatography-time of flight (GC/TOF) mass spectrometry-based metabolomic profile were monitored in blood, ileum, cecum, colon, and feces in gnotobiotic mice. The potential anti-inflammatory metabolites were tested in vitro. We obtained stable E. coli and F. prausnitzii-diassociated mice in which E. coli primed the gastrointestinal tract (GIT), allowing a durable and stable establishment of F. prausnitzii. The disease activity index, histological scores, myeloperoxidase (MPO) activity, and serum cytokine levels were significantly lower in the presence of F. prausnitzii after TNBS challenge. The protective effect of F. prausnitzii against colitis was correlated to its implantation level and was linked to overrepresented metabolites along the GIT and in serum. Among 983 metabolites in GIT samples and serum, 279 were assigned to known chemical reactions. Some of them, belonging to the ammonia (α-ketoglutarate), osmoprotective (raffinose), and phenolic (including anti-inflammatory shikimic and salicylic acids) pathways, were associated with a protective effect of F. prausnitzii, and the functional link was established in vitro for salicylic acid. We show for the first time that F. prausnitzii is a highly active commensal bacterium involved in reduction of colitis through in vivo modulation of metabolites along the GIT and in the peripheral blood. IMPORTANCE Inflammatory bowel diseases (IBD) are characterized by low proportions of F. prausnitzii in the gut microbiome. This commensal bacterium exhibits anti-inflammatory effects through still unknown mechanisms. Stable monoassociated rodents are actually not a reproducible model to decipher F. prausnitzii protective effects. We propose a new gnotobiotic rodent model providing mechanistic clues. In this model, F. prausnitzii exhibits protective effects against an acute colitis and a protective metabolic profile is linked to its presence along the digestive tract. We identified a molecule, salicylic acid, directly involved in the protective effect of F. prausnitzii. Targeting its metabolic pathways could be an attractive therapeutic strategy in IBD.

We have introduced a new hybrid fabrication method for lab-on-a-chip devices through the combination of femtosecond laser micromachining and removable insert micro-injection molding. This method is particularly suited for the fast prototyping of new devices, while maintaining a competitive low cost. To demonstrate the effectiveness of our approach, we designed, fabricated, and tested a completely integrated flow cytometer coupled to a portable media device. The system operation was tested with fluorescent plastic micro-bead solutions ranging from 100 beads/μL to 500 beads/μL. We demonstrated that this hybrid lab-on-a-chip fabrication technology is suitable for producing low-cost and portable biological microsystems and for effectively bridging the gap between new device concepts and their mass production.

The 3' untranslated region (3'UTR) of human astroviruses (HAstV) consists of two hairpin structures (helix I and II) joined by a linker harboring a conserved PTB/hnRNP1 binding site. The identification and characterization of cellular proteins that interact with the 3'UTR of HAstV-8 virus will help to uncover cellular requirements for viral functions. To this end, mobility shift assays and UV cross-linking were performed with uninfected and HAstV-8-infected cell extracts and HAstV-8 3'UTR probes. Two RNA-protein complexes (CI and CII) were recruited into the 3'UTR. Complex CII formation was compromised with cold homologous RNA, and seven proteins of 35, 40, 45, 50, 52, 57/60 and 75 kDa were cross-linked to the 3'UTR. Supermobility shift assays indicated that PTB/hnRNP1 is part of this complex, and 3'UTR-crosslinked PTB/hnRNP1 was immunoprecipitated from HAstV-8 infected cell-membrane extracts. Also, immunofluorescence analyses revealed that PTB/hnRNP1 is distributed in the nucleus and cytoplasm of uninfected cells, but it is mainly localized perinuclearly in the cytoplasm of HAstV-8 infected cells. Furthermore, the minimal 3'UTR sequences recognized by recombinant PTB are those conforming helix I, and an intact PTB/hnRNP1-binding site. Finally, small interfering RNA-mediated PTB/hnRNP1 silencing reduced synthesis viral genome and virus yield in CaCo2 cells, suggesting that PTB/hnRNP1 is required for HAstV replication. In conclusion, PTB/hnRNP1 binds to the 3'UTR HAstV-8 and is required or participates in viral replication.

The cobas 4800 BRAF V600 Mutation Test is a CE-marked and FDA-approved in vitro diagnostic assay used to select patients with metastatic melanoma for treatment with the selective BRAF inhibitor vemurafenib. We describe the pre-approval validation of this test in two external laboratories.Melanoma specimens were tested for BRAF V600 mutations at two laboratories with the: cobas BRAF Mutation Test; ABI BRAF test; and bidirectional direct sequencing. Positive (PPA) and negative (NPA) percent agreements were determined between the cobas test and the other assays. Specimens with discordant results were tested with massively parallel pyrosequencing (454). DNA blends with 5% mutant alleles were tested to assess detection rates.Invalid results were observed in 8/116 specimens (6·9%) with Sanger, 10/116 (8·6%) with ABI BRAF, and 0/232 (0%) with the cobas BRAF test. PPA was 97·7% for V600E mutation for the cobas BRAF test and Sanger, and NPA was 95·3%. For the cobas BRAF test and ABI BRAF, PPA was 71·9% and NPA 83·7%. For 16 cobas BRAF test-negative/ABI BRAF-positive specimens, 454 sequencing detected no codon 600 mutations in 12 and variant codon 600 mutations in four. For eight cobas BRAF test-positive/ABI BRAF-negative specimens, four were V600E and four V600K by 454 sequencing. Detection rates for 5% mutation blends were 100% for the cobas BRAF test, 33% for Sanger, and 21% for the ABI BRAF. Reproducibility of the cobas BRAF test was 111/116 (96%) between the two sites.It is feasible to evaluate potential companion diagnostic tests in external laboratories simultaneously to the pivotal clinical trial validation. The health authority approved assay had substantially better performance characteristics than the two other methods. The overall success of the cobas BRAF test is a proof of concept for future biomarker development.

El artículo presenta al teletrabajo como la última trasformación del trabajo a través de la historia del pensamiento económico. Se analiza desde diversos aspectos tales como su concepto y propuesta de clasificación, el derecho laboral, la motivación y el perfil de los teletrabajadores así como sus áreas de aplicación. El teletrabajo es una tendencia laboral actual que está trasformando la idea de que el ser humano debe salir de casa y trasladarse físicamente a un lugar para trabajar, hoy día el trabajo puede realizarse en cualquier lugar en el que se cuente con computadora e internet, este hecho requiere analizar los cambios en materia económica, administrativa y jurídica que conlleva.