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Background The objective of this work was to engineer Deinococcus radiodurans R1 as a microbial cell factory for the production of pinene, a monoterpene molecule prominently used for the production of fragrances, pharmaceutical products, and jet engine biofuels. Our objective was to produce pinene from glycerol, an abundant by-product of various industries. Results To enable pinene production in D. radiodurans, we expressed the pinene synthase from Abies grandis, the geranyl pyrophosphate (GPP) synthase from Escherichia coli, and overexpressed the native 1-deoxy-d-xylulose 5-phosphate synthase. Further, we disrupted the deinoxanthin pathway competing for the substrate GPP by either inactivating the gene dr0862, encoding phytoene synthase, or substituting the native GPP synthase with that of E. coli. These manipulations resulted in a D. radiodurans strain capable of producing 3.2 ± 0.2 mg/L pinene in a minimal medium supplemented with glycerol, with a yield of 0.13 ± 0.04 mg/g glycerol in shake flask cultures. Additionally, our results indicated a higher tolerance of D. radiodurans towards pinene as compared to E. coli. Conclusions In this study, we successfully engineered the extremophile bacterium D. radiodurans to produce pinene. This is the first study demonstrating the use of D. radiodurans as a cell factory for the production of terpenoid molecules. Besides, its high resistance to pinene makes D. radiodurans a suitable host for further engineering efforts to increase pinene titer as well as a candidate for the production of the other terpenoid molecules.

Extreme tree growth reductions represent events of abrupt forest productivity decline and carbon sequestration reduction. An increase in their magnitude can represent an early warning signal of impending tree mortality. Yet the long-term trends in extreme growth reductions remain largely unknown. We analyzed the trends in the proportion of trees exhibiting extreme growth reductions in two Central-European conifer species-Pinus sylvestris (PISY) and Picea abies (PCAB)-between 1901 and 2018. We used a novel approach for extreme growth reduction quantification by relating their size to their mean recurrence interval. Twenty-eight sites throughout Czechia and Slovakia with 1120 ring width series representing high- and low-elevation forests were inspected for extreme growth reductions with recurrence intervals of 15 and 50 years along with their link to climatic drivers. Our results show the greatest growth reductions at low-elevation PCAB sites, indicating high vulnerability of PCAB to drought. The proportions of trees exhibiting extreme growth reductions increased over time at low-elevation PCAB, decreased recently following an abrupt increase in the 1970-1980s at high-elevation PCAB, and showed nonsignificant trends in high- and low-elevation PISY. Climatic drivers of extreme growth reductions, however, shifted over time for all site categories as the proportion of low-temperature-induced extreme growth reductions declined since the 1990s, whereas events caused by drought consistently increased in frequency during the same period. We observed higher growth volatility at the lower range of distribution compared with the upper range margin of PISY and PCAB. This will undoubtedly considerably impact tree growth and vitality as temperatures and incidence of drought in Central Europe are expected to further increase with ongoing climate change.

Rationale The polyprenols are involved in some essential biosynthetic pathways and serve as ubiquitous components of cellular membranes, so their fingerprinting in natural samples is of great interest. Previous studies indicate that due to the high hydrophobicity of polyprenols their direct analysis by mass spectrometry with soft ionization techniques may be difficult and require preliminary off-line derivatization. Hence, a method for rapid and sensitive screening of polyprenols is required. Methods A combination of thin-film chemical deposition and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) was used for analysis of the polyprenol profile of Abies sibirica L. extract. Polyprenol-based monolayers were formed at the interphase of aqueous barium acetate solution, supplemented with 2,5-dihydroxybenzoic acid, and an n-hexane solution of polyprenols directly on a MALDI target plate. Results Peaks corresponding to [M - H + Ba]+ ions were observed in the MALDI-TOF mass spectra of polyprenols. A total of nine polyprenol homologues were identified with a polyprenol of 16 isoprene units dominating. The limit of detection was established at the level of 6 pg. Possible mechanisms of formation of [M - H + Ba]+ ions of polyprenols were discussed. Conclusions The proposed approach can be suitable for high-throughput screening of polyprenols in biological samples of different origin due to easy sample preparation and high sensitivity.

In this study, we describe the legacy effects of a soil sulfur amendment experiment performed 6 years prior and the resulting alterations to the rhizosphere communities of fir trees on a Christmas tree plantation. The pH of bulk soil was ∼1.4 pH units lower than that of untreated soils and was associated with reduced Ca, Mg, and organic matter contents. Similarly, root chemistry differed due to the treatment, with roots in sulfur-amended soils showing significantly higher Al, Mn, and Zn contents and reduced levels of B and Ca. 16S rRNA and 18S rRNA gene sequencing was pursued to characterize the bacterial/archaeal and eukaryotic communities in the rhizosphere soils. The treatment induced dramatic and significant changes in the microbial populations, with thousands of 16S rRNA gene sequence variants and hundreds of 18S rRNA gene variants being significantly different in relative abundances between the treatments. Additionally, co-occurrence networks showed that bacterial and eukaryotic interactions, network topology, and hub taxa were significantly different when constructed from the control and treated soil 16S and 18S rRNA gene amplicon libraries. Metagenome sequencing identified several genes related to transport proteins that differentiated the functional potentials of the communities between treatments, pointing to physiological adaptations in the microbial communities for living at altered pH. These data show that a legacy of soil acidification increased the heterogeneity of the soil communities as well as decreasing taxon connections, pointing to a state of ecosystem instability that has potentially persisted for 6 years. IMPORTANCE We used sulfur incorporation to investigate the legacy effects of lowered soil pH on the bacterial and eukaryotic populations in the rhizosphere of Christmas trees. Acidification of the soils drove alterations of fir tree root chemistry and large shifts in the taxonomic and functional compositions of the communities. These data demonstrate that soil pH influences are manifest across all organisms inhabiting the soil, from the host plant to the microorganisms inhabiting the rhizosphere soils. Thus, this study highlights the long-lasting influence of altering soil pH on soil and plant health as well as the status of the microbiome.

Key message Model simulation results suggest that forests in the Sierra Nevada mountains of California will tend to increase in density and basal area in the absence of fire over the next century, and that climate change will favor increases in drought-tolerant species. Context Climate change is projected to intensify the natural summer drought period for Mediterranean-climate forests. Such changes may increase tree mortality, change species interactions and composition, and impact ecosystem services. Aims To parameterize SORTIE-ND, an individual-based, spatially explicit forest model, for forests in the Sierra Nevada, and to model forest responses to climate change. Methods We use 3 downscaled GCM projections (RCP 8.5) to project forest dynamics for 7 sites at different elevations. Results Basal area and stem density tended to increase in the absence of fire. Climate change effects differed by species, with more drought-tolerant species such as Jeffrey pine (Pinus jeffreyi A.Murray bis) and black oak (Quercus kelloggii Newb.) exhibiting increases in basal area and/or density. Conclusion Increasing forest density may favor carbon sequestration but could increase the risk of high-severity fires. Future analyses should include improved parameterization of reproduction and interactions of disturbance with climate effects.

Due to their wide distribution and availability, plant leaves can be considered interesting candidates as biomonitoring substrates for the evaluation of atmospheric pollution. In addition, some species can also retain historical information, for example, related to environmental pollution, due to their leaf class age. In this study, the content of polycyclic aromatic hydrocarbons (PAHs) in Abies holophylla and Pinus tabuliformis needle samples in function of their class age has been investigated to obtain information regarding the degradation constant for each PAH under investigation (α values ranging from 0.173 to 1.870) and to evaluate the possibility to correlate the presence of PAHs in needles with some important pollution environmental factors. Considering air pollutant variables registered in Jilin Province, significant correlations (at 95% confidence level) have been found between coal consumption per year and anthracene contents in needles, while fluorene, phenanthrene, and anthracene resulted correlated with coal consumption. Furthermore, it has been demonstrated that the total PAH concentration in needles, for both species, increased with their age (from 804 to 3604 ng g− 1 dry weight), showing a general tendency to accumulate these substances through years. PAH degradation rates increased instead with molecular complexity. This study could be considered a first trial to obtain historical environmental information by pine needles biomonitoring.

Climate change-related environmental stress has been recognized as a driving force in accelerating forest mortality over the last decades in Central Europe. Here, we aim to elucidate the thermal sensitivity of three native conifer species, namely Norway spruce (Picea abies), Scots pine (Pinus sylvestris) and silver fir (Abies alba), and three non-native species, namely Austrian pine (Pinus nigra), Douglas fir (Pseudotsuga menziesii) and Atlas cedar (Cedrus atlantica). Thermal sensitivity, defined here as a decline of the maximum quantum yield of photosystem II (Fv /Fm ) with increasing temperature, was measured under varying levels of heat stress and compared with the turgor loss point (πtlp ) as a drought resistance trait. We calculated three different leaf thermotolerance traits: the temperature at the onset (5%) of the Fv /Fm decline (T5), the temperature at which Fv /Fm was half the maximum value (T50) and the temperature at which only 5% Fv /Fm remained (T95). T5 ranged from 38.5 ± 0.8 °C to 43.1 ± 0.6 °C across all species, while T50 values were at least 9 to 11 degrees above the maximum air temperatures on record for all species. Only Austrian pine had a notably higher T5 value than recorded maximum air temperatures. Species with higher T5 values were characterized by a less negative πtlp compared to species with lower T5. The six species could be divided into 'drought-tolerant heat-sensitive' and 'drought-sensitive heat-tolerant' groups. Exposure to short-term high temperatures thus exhibits a considerable threat to conifer species in Central European forest production systems.

Plant nitrogen (N) uptake is affected by plant–plant interactions, but the mechanisms remain unknown. A 15N-labeled technique was used in a pot experiment to analyze the uptake rate of ammonium (NH4+) and nitrate (NO3−) by Abies faxoniana Rehd. et Wils and Picea asperata Mast. in single-plant mode, intraspecific and interspecific interactions. The results indicated that the effects of plant–plant interactions on N uptake rate depended on plant species and N forms. Picea asperata had a higher N uptake rate of both N forms than A. faxoniana, and both species preferred NO3−. Compared with single-plant mode, intraspecific interaction increased NH4+ uptake for A. faxoniana but reduced that for P. asperata, while it did not change NO3− uptake for the two species. The interspecific interaction enhanced N uptake of both N forms for A. faxoniana but did not affect the P. asperata compared with single-plant mode. NH4+ and NO3− uptake rates for the two species were regulated by root N concentration, root nitrate reductase activity, root vigor, soil pH and soil N availability under plant–plant interactions. Decreased NH4+ uptake rate for P. asperata under intraspecific interaction was induced by lower root N concentration and nitrate reductase activity. The positive effects of interspecific interaction on N uptake for A. faxoniana could be determined mainly by positive rhizosphere effects, such as high soil pH. From the perspective of root–soil interactions, our study provides insight into how plant–plant interactions affect N uptake, which can help to understand species coexistence and biodiversity maintenance in forest ecosystems.

(1) Background: The genetic basis of local adaptation in conifers remains poorly understood because of limited research evidence and the lack of suitable genetic materials. Sakhalin fir (Abies sachalinensis) is an ideal organism for elucidating the genetic basis of local adaptation because its altitudinal adaptation has been demonstrated, and suitable materials for its linkage mapping are available. (2) Method: We constructed P336 and P236 linkage maps based on 486 and 516 single nucleotide polymorphisms, respectively, that were derived from double digest restriction site-associated DNA sequences. We measured the growth and eco-physiological traits associated with morphology, phenology, and photosynthesis, which are considered important drivers of altitudinal adaptation. (3) Results: The quantitative trait loci (QTLs) for growth traits, phenology, needle morphology, and photosynthetic traits were subsequently detected. Similar to previous studies on conifers, most traits were controlled by multiple QTLs with small or moderate effects. Notably, we detected that one QTL for the crown area might be a type-A response regulator, a nuclear protein responsible for the cytokinin-induced shoot elongation. (4) Conclusion: The QTLs detected in this study include potentially important genomic regions linked to altitudinal adaptation in Sakhalin fir.

The oyamel forests, as Abies dominated forests are commonly known as, register their largest distribution (95% of their population) along the Trans-Mexican Volcanic Belt (TMVB). Although efforts have been made to study these forests with various approaches, dendrochronology-based studies have been limited, particularly in pure Abies forests in this region. The objective of this study was to reconstruct fire regimes in an Abies religiosa forest in the Sierra Norte in the state of Puebla, Mexico. Within an area of 50-ha, we collected 40 fire-scar samples, which were processed and analyzed using dendrochronological techniques to identify 153 fire scars. The fire history was reconstructed for a period of 118 years (1896–2013), with low severity surface fires occurring mainly during in the spring (92.8%) and summer (7.2%). Over the past century, fires were frequent, with an mean fire interval (MFI) and Weibull median probability of (WMPI) of five years when considering all fire scars and less than 10 years for fires covering larger areas (fires recorded by ≥25% of samples). Extensive fires were synchronized with drought conditions based on Ring Width Indexes, Palmer Drought Severity Index (PDSI) and El Niño Southern Oscillation (ENSO). After 1983, we observed a change in fire frequencies attributed to regulated management. Longer fire intervals within the last several decades are likely leading to increased fuel accumulations and could potentially result in more severe fires in the future, threatening the sustainability of these forests. Based on our finding, we recommend management actions (silvicultural or prescribed fire) to reduce fuels and the risk of severe fires, particularly in the face of climatic changes.

After drought-induced embolism and repair, tree xylem may be weakened against future drought events (cavitation fatigue). As there are few data on cavitation fatigue in conifers available, we quantified vulnerability curves (VCs) after embolism/repair cycles on eight European conifer species. We induced 50% and 100% loss of conductivity (LC) with a cavitron, and analyzed VCs. Embolism repair was obtained by vacuum infiltration. All species demonstrated complete embolism repair and a lack of any cavitation fatigue after 50% LC . After 100% LC, European larch (Larix decidua), stone pine (Pinus cembra), Norway spruce (Picea abies), and silver fir (Abies alba) remained unaffected, while mountain pine (Pinus mugo), yew (Taxus baccata), and common juniper (Juniperus communis) exhibited 0.4–0.9 MPa higher vulnerability to embolism. A small cavitation fatigue observed in Scots pine (Pinus sylvestris) was probably biased by incomplete embolism repair, as indicated by a correlation of vulnerability shifts and conductivity restoration. Our data demonstrate that cavitation fatigue in conifers is species-specific and depends on the intensity of preceding LC. The lack of fatigue effects after moderate LC, and relevant effects in only three species after high LC, indicate that conifers are relatively resistant against cavitation fatigue. This is remarkable considering the complex and delicate conifer pit architecture and may be important considering climate change projections.

Climate change is shifting forest tree species distributions across elevational and latitudinal gradients, and these changes are often pronounced at ecotones where species meet their climatic bounds and are replaced by other species. Using an extensive ecotone composed of lower-montane white fir (Abies concolor var. lowiana) and upper-montane red fir (Abies magnifica var. magnifica) in the central Sierra Nevada range of California, USA, we (1) examined how the demographics of the ecotone have responded to recent climate using a field observational study and a historical dataset, (2) quantified climate drivers across species life stages using contemporary demographic data, and (3) tested the potential impacts of future climate on species-specific seedling survival and growth in a fully factorial growth chamber experiment that varied temperature, growing season length, and water availability. A re-examination of the ecotone midpoint after 35 yr suggested a reduction in A. concolor sapling and tree densities and a rise in A. magnifica proportional dominance between surveys. Seedling abundances across the ecotone indicated that A. magnifica tends to dominate the regeneration layer and currently forms an important component of the seedling community at elevations below those where A. magnifica saplings or trees begin to co-dominate stands. Observational and experimental assessments suggest that temperature and precipitation serve as important drivers, differentiating A. concolor vs. A. magnifica distributions, and are primary stressors at the seedling stage. Seedlings of both species were adversely affected by experimental climate treatments, although A. concolor exhibited greater survival and a more conservative growth strategy under extreme climatic stress than A. magnifica. Projections indicate that historical climate conditions will rise by an amount greater than the ecotone's current elevational extent by the end of the 21st century. Differential drivers of species abundances suggest that the projected climate will expand conditions that promote A. concolor abundance and impede A. magnifica abundance across the ecotone; however, disturbance activity and microclimatic conditions will also influence regeneration and overstory tree dynamics. Our study demonstrates the importance of quantifying species-specific responses to climate and indicates that widespread regeneration failure may be one possible consequence in which species exhibit strong sensitivity to projected climate conditions.

Plant carbon (C) assimilation is expected to nonlinearly increase with continuously increasing nitrogen (N) deposition, causing a N saturation threshold for productivity. However, the response of plant productivity to N deposition rates and further the N saturation threshold still await comprehensive quantization for forest ecosystem. Here, we tested the effect of N addition on aboveground net primary productivity (ANPP) of three-year old Chinese fir (Cunninghamia lanceolata) trees by adding N at 0, 5.6, 11.2, 22.4, and 44.8 g N m

House dust mite (HDM) is a globally ubiquitous domestic cause of allergic diseases. There is a pressing demand to discover efficient, harmless, and eco-friendly natural extracts to inhibit HDM allergens that are more likely to trigger allergies and challenging to be prevented entirely. This study, therefore, is aimed at assessing the inhibition of the allergenicity of major HDM allergen Der f 2 by todomatsu oil extracted from residues of Abies Sachalinensis. The inhibition was investigated experimentally (using enzyme-linked immunosorbent assay (ELISA), surface plasmon resonance (SPR), and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)) and in silico using molecular docking. The results showed that todomatsu oil inhibits the allergenicity of Der f 2 by reducing its amount instead of the IgG binding capacity of a single protein. Moreover, the compounds in todomatsu oil bind to Der f 2 via alkyl hydrophobic interactions. Notably, most compounds interact with the hydrophobic amino acids of Der f 2, and seven substances interact with CYS27. Contrarily, the principal compounds fail to attach to the amino acids forming the IgG epitope in Der f 2. Interestingly, chemical components with the lowest relative percentages in todomatsu oil show high-affinity values on Der f 2, especially β-maaliene (−8.0 kcal/mol). In conclusion, todomatsu oil has been proven in vitro as a potential effective public health strategy to inhibit the allergenicity of Der f 2.

Species of the genus Tuber have gained a lot of attention in recent decades due to their aromatic hypogenous fruitbodies, which can bring high prices on the market. The tendency in truffle production is to infect oak, hazel, beech, etc. in greenhouse conditions. We aimed to show whether silver fir (Abies alba Mill.) can be an appropriate host partner for commercial mycorrhization with truffles, and how earthworms in the inoculation substrate would affect the mycorrhization dynamics. Silver fir seedlings inoculated with Tuber. aestivum were analyzed for root system parameters and mycorrhization, how earthworms affect the bare root system, and if mycorrhization parameters change when earthworms are added to the inoculation substrate. Seedlings were analyzed 6 and 12 months after spore inoculation. Mycorrhization with or without earthworms revealed contrasting effects on fine root biomass and morphology of silver fir seedlings. Only a few of the assessed fine root parameters showed statistically significant response, namely higher fine root biomass and fine root tip density in inoculated seedlings without earthworms 6 months after inoculation, lower fine root tip density when earthworms were added, the specific root tip density increased in inoculated seedlings without earthworms 12 months after inoculation, and general negative effect of earthworm on branching density. Silver fir was confirmed as a suitable host partner for commercial mycorrhization with truffles, with 6% and 35% mycorrhization 6 months after inoculation and between 36% and 55% mycorrhization 12 months after inoculation. The effect of earthworms on mycorrhization of silver fir with Tuber aestivum was positive only after 6 months of mycorrhization, while this effect disappeared and turned insignificantly negative after 12 months due to the secondary effect of grazing on ectomycorrhizal root tips.

Phenological shifts, induced by global warming, can lead to mismatch between closely interacting species. The eastern spruce budworm, Choristoneura fumiferana, an important outbreaking insect defoliator in North America, mainly feeds on balsam fir, Abies balsamea, which has historically been well synchronized with the insect. But as climate change pushes the northern range limit of the budworm further north into the boreal forest, the highly valuable black spruce, Picea mariana, historically protected against the budworm by its late budburst phenology, is suffering increased defoliation during the current outbreak. We tested the hypothesis that rising temperatures can lead, not to a mismatch, but to an improved match between the budworm and black spruce through differential phenological advancement. For 3 years, eastern spruce budworm larvae were reared from instar 2 to pupae, on both black spruce and balsam fir, in a temperature free-air controlled enhancement experiment (T-FACE) consisting in 24 field plots, half of which were heated at +2°C from March to October. Our results show that every year, larval development was faster on heated trees and pupation was earlier than on unheated trees. Bud development was also accelerated in heated trees of both species. However, there was no difference in mass between pupae that developed at +2°C and controls at the end of the season. Finally, we found no difference either in development rate or pupal mass between larvae reared on black spruce and those reared on balsam fir. This suggests that under higher temperature regimes, eastern spruce budworm will be as successful on black spruce as on balsam fir, as black spruce budburst becomes better synchronized with the insect's emergence from diapause. This could lead to critical changes in outbreak dynamics and severity with important ecological state shifts at the landscape level.

Chinese fir is one of the most important commercial timber species in China, with many geographic sources. However, little is known of the variation in wood physical properties among them. To explore the differences in wood physical properties and their influencing factors, five geographic sources of Chinese fir were selected. The variance inflation factor, stepwise regression, and principle component analysis were used to reduce multicollinearity and dimensions of the 19 wood physical properties (including density, shrinkage, and mechanical properties). The results showed that the wood density differed significantly among five geographic sources. The tangential shrinkage rate and radial shrinkage rate reached maximum values in black-heart Chinese fir (HNYX-T) but accompanied by the lowest value for difference dry shrinkage. The wood density and mechanical properties of HNYX-T was exceeded to that of others geographic sources. Fast-growth Chinese fir (FJYK-P) had the lowest value for all mechanical properties. The precipitation and temperature had significant correlations with the wood physical properties of this five geographic sources. The temperature in summer was mainly positive correlated with physical properties, while precipitation was negatively correlated with them. HNYX-T had the highest comprehensive score of PCA, followed by JXCS-R, emerged as higher-quality geographic source, which is important for selecting and utilizing geographic sources in forest management.

For decades, one of the most popular ideas in reducing the burden of cancer worldwide is the idea of cancer chemoprevention with low-toxic agents. There is an evidence that polyprenols (polyisoprenoid alcohols) are perspective agents for cancer chemoprevention.

Bark beetles attack their hosts at uniform intervals to avoid intraspecific competition in the phloem. Bark texture and phloem thickness also affect bark beetle attacks, and the bark characteristics are not spatially homogeneous; therefore, the distribution patterns of entry holes can demonstrate an aggregated distribution. Polygraphus proximus Blandford (Coleoptera: Scolytinae) is a non-aggressive phloephagous bark beetle that feeds on Far Eastern firs. They have caused mass mortality in Russia and Japan. However, the distribution pattern of entry holes of P. proximus and spatial relationships with bark characteristics have not been studied. Thus, we investigated the distribution pattern of entry holes of P. proximus. The distribution of entry holes was significantly uniform in most cases. As the attack density increased, an aggregated distribution pattern within a short distance (< 4.0 cm) was observed. The rough bark had a significantly higher number of entry holes than the remaining bark. The distribution pattern of entry holes demonstrated a significantly aggregated spatial association with rough bark. Finally, rough bark around knots had significantly thicker phloem than the remaining barks. These suggest that P. proximus may preferentially attack rough bark to reproduce in the thicker phloem under a rough bark surface.

Variability in functional traits (FT) is increasingly used to understand the mechanisms behind tree species interactions and ecosystem functioning. In order to explore how FT differ due to interactions between tree species and its influence on stand productivity and other ecological processes, we examined the effects of tree species composition on the intra-specific variability of four widely measured FT: specific leaf area, leaf nitrogen content, leaf angle and stomatal conductance response to vapor pressure deficit. This study focused on three major central European tree species: European beech (Fagus sylvatica L.), Sessile oak (Quercus petraea Liebl.) and Norway spruce (Picea abies [L.] H. Karst.). Each species was examined in monoculture and two-species mixtures in the 13-year-old tree biodiversity experiment BIOTREE-Kaltenborn. Trait distributions and linear mixed models were used to analyze the effect of species mixing, tree size and stand variables on the intra-specific FT variability. A significant effect of branch height on most traits and species indicated a vertical gradient of foliar trait frequently related to light availability. Beech and oak showed a high overall trait variability and sensitivity to species mixing and stand basal area, while the trait variability of spruce was limited. Greater shifts in trait distributions due to mixing were found in specific leaf area for oak and leaf nitrogen content for beech. Thus intra-specific variability of key leaf traits was already influenced at this young development stage by inter-specific interactions. Finally, we used the 3-PG (Physiological Processes Predicting Growth) process-based forest growth model to show that the measured intra-specific variability on single FT values could influence stand productivity, light absorption and transpiration, although the net effect depends on the considered trait and the species composition of the mixture. The results of this study will aid better understanding of the effects of inter-specific competition on intra-specific FT variability, which has implications for the parameterization of process-based forest growth models and our understanding of ecosystem functioning.

The spatial patterns of plant species reflect the competitive pressures on individuals. To generate Competition indices (CI), we measured the diameter at breast height (DBH), crown volumes (CV) and the distances between trees. In this study, Abies kawakamii were divided based on the dominant component of the understory (moss or bamboo) to (1) investigate the relationship between the CI and stand structural attributes (SSAs); (2) compare the inter- and intraspecies; CIs as well as living and dead individual CIs; and (3) examine the relationship between the DBH and CI. The current findings indicate that the understory composition affected the CI and SSAs. The interspecies CI was larger than the intraspecies CI when bamboo-dominated the understory. In contrast, the intraspecies CI was larger than the interspecies CI when the understory was dominated by moss. The CI of dead individuals was higher than that of live individuals due to the biological characteristics and regeneration needs of Abies. Additionally, sensitivity to the environment and available resources may exert more pressure on young individuals than mature individuals.

The results of this research are part of the contract for Formacion de Profesorado Universitario (University Teacher Training) (Ref. 18/03023) financed by the Ministerio de Educacion del Gobierno de Espana (Ministry of Education of the Government of Spain), and research projects PALEOPINSAPO (CSO2017-83576-P), Med-Refugia (Ref. RTI2018-101714-B-I00), Oromed-Refugia (Ref. P18-RT-4963) and Relic-Flora 2 (Ref. B-RNM-404-UGR18), dependents on the Ministerio de Economia y Competitividad del Gobierno de Espana y la Junta de Andalucia (Ministry of the Economy and Competitiveness of the Government of Spain and the Regional Government of Andalusia., The aim of this research is to reconstruct the ancient distribution area of Abies pinsapo Boiss. (Spanish fir) in the Serranía de Ronda region, southern Spain, during the Holocene. The main method was pedoanthracological analysis, the study of non-archaeological charcoal found in natural soils. In this research a total of 37 soil excavations were done in several mountain ranges with potentially favourable places for firs to have grown in the past. Specific sites and places such as hillsides, endorheic basins (with no outflow), sinkholes, summits and mountain passes were selected on the basis of evidence from a range of different sources including ancient documents, pollen studies and species distribution models. The soil samples collected from these sites were prepared in the laboratory and the charcoal was identified and radiocarbon dated. Statistical and cartographic analyses were also done. The study revealed evidence of past populations of Abies sp. in places where it is no longer found today. A total of 47 different chronologies were obtained from these sites with ages ranging between 9,931 cal bp and 78 cal bp. In addition, the wide variations in the charcoal values enabled us to make an initial estimate of the importance of ancient forest fires in different places in the Serranía de Ronda. When this information has been considered with all the other available data sources, it will be an essential resource for the efficient management of relict fir woods in southern Spain., Ministerio de Educacion del Gobierno de Espana (Ministry of Education of the Government of Spain) 18/03023, dependents on the Ministerio de Economia y Competitividad del Gobierno de Espana y la Junta de Andalucia (Ministry of the Economy) CSO2017-83576-P RTI2018-101714-B-I00 P18-RT-4963 B-RNM-404-UGR18, Junta de Andalucia, Competitiveness of the Government of Spain

International audience; We used pollen, plant macrofossil, and charcoal records to investigate local long-term timberline shifts and changes in vegetation composition in relation to fire activity at the modern upper forest limit (ca. 2,000 m a.s.l.) in the Mont Bégo area, Maritime Alps of France and Italy. The area is an important place for Alpine archaeology because it has thousands rock-art carvings whose age cannot be directly assessed. Our new record confirms the occurrence of distinct land use phases (7,450–7,150, 6,200–4,900, and 4,250–3,700 cal bp), as suggested by earlier studies of rock art typology. Moreover, the vegetation reconstruction from macrofossils, with co-dominance of Pinus and Betula, suggests that early Holocene conditions were moister than in drier inner Alpine valleys, where Larix decidua played a more important role, both in the past as well as in modern timberline forests. After 8,000 cal bp, the timberline shifted upwards and mixed Abies alba and Pinus cembra stands established around the study site. These fire sensitive trees were finally replaced during the Bronze Age (around 4,000 cal bp) by L. decidua, which still dominates the subalpine woodlands in the area today. Our study supports the notion that while the range of A. alba has been reduced at the colder end of its natural distribution, that of L. decidua has been widened by land use changes and fire disturbances to create high alpine wood pastures.

We have previously reported that phytochemicals from Abies holophylla exhibit anti-inflammatory and neuroprotective effects by decreasing nitrite production and increasing nerve growth factor production. However, the exact mechanism underscoring these effects has not been revealed. In the present study, we aimed to explore the underlying anti-inflammatory mechanisms of A. holophylla and its phytochemicals. We studied various solvent fractions of A. holophylla and found the chloroform and hexane sub-fractions showed the most significant anti-neuroinflammatory effects in lipopolysaccharide (LPS)-activated murine microglia. Concomitantly, the terpenoids isolated from chloroform and hexane fractions showed similar anti-neuroinflammatory effects with significant inhibition of NO and reactive oxygen species production, and decreased protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase. Interestingly, these terpenoids inhibited the phosphorylation of c-Jun N-terminal kinase (JNK), which further inhibited the production of pro-inflammatory mediators, including prostaglandin E2, tumor necrosis factor, and interleukins (IL-6 and IL-1&beta, ), with a potency greater than that of the well-known iNOS inhibitor NG-mono-methyl-L-arginine (L-NMMA). These results suggest that the chloroform- and hexane-soluble fraction mediated the mitogen-activated protein kinase (MAPK) inhibition, in particular the JNK pathway, thereby lowering the inflammatory cascades in LPS-activated murine microglia. Thus, our study suggests that the chloroform and hexane fractions of A. holophylla and their terpenoids may be potential drug candidates for drug discovery against LPS-induced neuroinflammation and neuroinflammatory-related neurodegeneration.

Historical distribution of fir (Abies alba) in the southern Pyrenees is thought to be wider thas present times, as revealed by combining information from one boring made at el Berguedi with previous results from la Garrotxa. A partir dels resultats d'un sondeig efectuat al Berguedi com també d'altres dades pol·liniques de la comarca de la Garrotxa, es patentitza una distribució de I'avet més amplia que I'actual en temps histbrics al vessant sud dels Pirineus.

Ectomycorrhizal fungi are an important group of symbiotic fungi beneficial to plant growth and their environmental adaptation. An explicit clarification of the trait of ectomycorrhizal fungi would facilitate our understanding of plant responses to environmental change. We set up sampling plots at five elevations (2850, 3000, 3194, 3413, 3593 m) in the Balong Mountain within the Wolong Nature Reserve of Sichuan Province, and collected cubic soil samples (10 cm×10 cm×10 cm) from those plots by point centered quarter method. Based on examination of the morphological types and diversity of ectomycorrhizal roots of外生菌根真菌是一类有助于植物生长及应对环境变化的重要共生真菌类群。深入认识外生菌根特征有助于理解植物对环境变化的响应规律。本研究在四川省卧龙自然保护区巴郎山脉设置海拔2850、3000、3194、3413和3593 m 5个样地,利用中心象限法进行土块样品(10 cm×10 cm×10 cm)采集,通过分析各海拔每土块中岷江冷杉外生菌根形态个数、形态多样性及养分获取类型等指标,研究岷江冷杉外生菌根形态特性随海拔的变异特征及土壤环境因子的作用。结果表明: 1) 菌根形态为橘黄色或橘色、单轴羽状分支或不分支、菌套表面光滑、无或少见外延物、根尖直筒型或膨大的菌根类型是卧龙自然保护区岷江冷杉外生菌根的主要形态类型。该形态类型在研究区内的侵染率最高,为12.4%;2) 随着海拔的升高,岷江冷杉外生菌根形态多样性下降,每土块内的形态个数减少;3) 单因素方差分析结果表明,不同海拔上无外延菌丝或极少外延菌丝的紧密接触类型(CE)和短距离养分获取类型(SDE)外生菌根侵染率有显著差异,CE类型外生菌根侵染率随着海拔的升高而增加;4) 土壤环境因子对岷江冷杉外生菌根形态在海拔上的变异有一定的影响,冗余分析(RDA)表明,土壤全氮含量、全磷含量、土壤温度、土壤含水率、pH值及酸性磷酸酶活性对岷江冷杉外生菌根形态在海拔上的变异有显著影响,其中土壤全氮含量和土壤温度的影响最大,分别解释了岷江冷杉形态特征变异的5.4%和4.9%。本研究揭示了岷江冷杉外生菌根形态形成在海拔上的差异,为进一步研究亚高山针叶树种菌根策略对环境变化的响应机制提供了科学依据。.

Worldwide, increasing severity of droughts threatens to change forest ecosystem functioning and community structure. Understanding how forest resilience is determined by its two underlying components, resistance and recovery, will help elucidate the mechanisms of drought responses and help inform management strategies. However, drought responses are shaped by complex processes across different scales, including species-specific drought strategies, tree size, competition, local environmental conditions, and the intensity of the drought event. Here, we quantified the reduction in tree growth during drought (an inverse measure of drought resistance) and post-drought recovery for three montane conifers (Abies concolor, Pinus jeffreyi, and Pinus lambertiana) in California. We used tree ring analysis to quantify responses to drought events of varying intensity between 1895 and 2018 across a geographic climatic gradient, to examine the roles of tree size (DBH) and competition (tree density) in mediating drought responses. We found that years of more intense drought corresponded with larger growth reductions and recovery rates were lower following drought years where trees suffered larger reductions. We found little variation among species in their growth reductions during drought events, but significant differences among species in their recovery post-drought. Across the geographic gradient, trees in the driest locations were susceptible to large growth reductions, signaling either strong sensitivity to drought intensity or exposure to the most extreme drought conditions. These growth reductions were not always compensated for by higher recovery rates. We also found that larger trees were more susceptible to drought due to a steeper negative relationship between recovery rates and the intensity of growth reduction during the drought. Contrary to expectations, recovery rates following the most detrimental drought years were higher in denser forests. Our results demonstrate the importance of considering how factors at various spatial and temporal scales affect the different components of drought responses.

A new

Dendrogeomorphic dating of past landslide events is a valuable tool for the assessment of landslide activity, providing unique data for the analysis of triggers or the modelling of landslide behaviour in the future. Unfortunately, tree-ring-based methods as well as dating approaches suffer from some limitations. One of the less frequently addressed limits of dendrogeomorphic analysis concerns the changing capacity of trees to record landslide events in their tree-ring series with increasing age. This study uses, to date, the most extensive database of tree-ring series (1736) of 868 disturbed individuals of Picea abies (L.) H. Karst. subjected to 20 landslides in the Outer Wester Carpathians for the assessment of their age-dependent sensitivity. The distribution of the total number of 1485 growth disturbances (reaction wood - RW and abrupt growth suppression - GS) throughout all decades of tree life shows evidence of distinct changes in the capacity for trees to record landslide signals with increasing age. The occurrence of RW dominated in the juvenile decades of tree life and then increased again in the 9th decade. The frequency of GS gradually increased and culminated during the 7th and 8th decades. The two intensities of growth disturbance (strong and moderate) expressed temporally balanced ratios, suggesting an effect of disturbance intensity rather than changing tree age. The important factors controlling age-dependent tree sensitivity to landslide movements based on the results seem to be changing stem elasticity, decreasing annual increment rates, root system development and increasing tree body weight. Moreover, this study demonstrates that landslide type (e.g., rigid block vs. plastic flow-like landslides) and bedrock geology distinctly modify age-dependent sensitivity that should be taken into account during the sampling of trees for landslide dating.

Soil arthropods (Arthropoda) in forest ecosystems play an important role as primary and secondary consumers in the process of litter fragmentation and decomposition. Amount, diversity, and community structure of arthropods in the soil ecosystem reacts very quickly to changes in habitat characteristics. In this study, it was aimed to reveal the seasonal changes, diversity, and effects of soil biological quality of soil-dwelling arthropods in Taurus Fir (Abies cilicica (Antoine & Kotschy) Carrière) ecosystem at different altitudes. The study was carried out in pure fir stands between 1600-1730 meters (3 different altitudes) in Berçin highland (Tomarza-Kayseri) forests. Sampling made from the litter using a cylinder core 10 cm diameter and height was performed in winter, spring, summer, and autumn seasons. The amount, diversity, trophic structure, and soil biological quality (QBS) values of arthropods were determined in seasonal and different altitudes. QBS abbreviations for soil biological quality based on arthropod (arthropoda; QBS-ar) and Collembola (Collembola; QBS-C) communities. As a result of the study, in the distribution of arthropods according to the seasons, the highest number of individuals was found in summer, and in the distribution depending on altitude, the highest number of individuals was found at an altitude of 1613 m. No statistical difference was found between elevations in terms of diversity index. Soil biological quality both QBS-ar and QBS-C value decrease along an elevation gradient. Identified Collembola species are new records for Kayseri/ Berçin Highland while Protaphorura microsellata species are new records for Turkey., Orman ekosistemlerindeki toprak eklembacaklıları (Arthropoda), ölüörtünün parçalanması ve ayrışması sürecinde, birincil ve ikincil tüketiciler olarak önemli rol almaktadır. Toprak ekosistemindeki eklembacaklıların miktarı, çeşitliliği ve komünite yapısı; yetişme ortamı özelliklerinin değişimine çok hızlı tepki vermektedir. Bu çalışmada, Toros Göknarı (Abies cilicica (Antoine & Kotschy) Carrière) ekosisteminde yaşayan toprak eklembacaklılarının farklı yükseltilerde mevsimsel değişimleri, çeşitlilikleri ve biyolojik toprak kalitesine olan etkilerinin ortaya konması amaçlanmıştır. Çalışma, Berçin Yaylası (Tomarza-Kayseri) saf göknar meşcerelerinde, 1600-1730 metre yükseltiler arasındaki (3 farklı yükseltide) yürütülmüştür. Ölü örtüden 10 cm çapında ve yüksekliğinde çelik silindir kullanılarak yapılan örnekleme kış, ilkbahar, yaz ve sonbahar mevsimlerinde yapılmıştır. Eklembacaklıların, mevsimsel ve farklı yükseltilerdeki miktarları, çeşitlilikleri, trofik yapıları ve toprak biyolojik kalitesi (QBS) değerleri belirlenmiştir. Toprak biyolojik kalitesi eklembacaklılar kullanılarak belirlenirse QBS-ar, Collembola taksonu kullanılarak belirlenirse QBS-C olarak adlandırılır. Çalışma sonucunda, eklembacaklıların mevsimlere göre dağılımında en fazla birey yaz mevsiminde, yükseltiye bağlı dağılımında ise en fazla birey 1613 m yükseltide bulunmuştur. Yükseltiler arasında çeşitlilik indeksi bakımından fark bulunamamıştır. Toprak biyolojik kalitesi hem QBS-ar hem de QBS-C değeri yükselti ile azalmaktadır. Teşhis edilen Collembola türleri Kayseri/ Berçin Yaylası için yeni kayıt iken Protaphorura microsellata türü Türkiye için yeni kayıttır.

Several plant polyphenols have been shown to reduce osteoarthritis symptoms due to their antioxidant, anti-inflammatory and immunomodulatory properties. We investigated the effects of two different polyphenolic extracts (Belinal, Pycnogenol) and two different polyphenols (resveratrol, quercetin) on the chondrogenic potential of bone-derived mesenchymal stem/stromal cells (MSCs) from healthy donors and patients with osteoarthritis. Our main aim was to determine whether Belinal, a commercially available polyphenolic extract from silver fir (Abies alba L.) branches, has comparable chondrogenic potential with the other tested extract and the polyphenols under inflammatory and non-inflammatory conditions. In our study, Belinal promoted significantly greater chondrogenesis compared to the untreated (p = 0.0289) and resveratrol-treated (p = 0.0468) MSCs from patients with hip osteoarthritis under non-inflammatory conditions. Under inflammatory conditions, chondrogenesis was significantly enhanced for MSCs treated with Belinal compared to the control (p = 0.0483). The other extract and the polyphenols did not show any significant effects on chondrogenesis under non-inflammatory or inflammatory conditions. None of the tested extracts and polyphenols showed significant effects on chondrogenesis in healthy donors, under either non-inflammatory or inflammatory conditions. Our data show that Belinal can boost the chondrogenesis of MSCs derived from patients with osteoarthritis, under both non-inflammatory and inflammatory conditions.