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Metabolic engineering of Deinococcus radiodurans for pinene production from glycerol
- Source :
- Helalat, S H, Jers, C, Bebahani, M, Mohabatkar, H & Mijakovic, I 2021, ' Metabolic engineering of Deinococcus radiodurans for pinene production from glycerol ', Microbial Cell Factories, vol. 20, no. 1, 187 . https://doi.org/10.1186/s12934-021-01674-4, Microbial Cell Factories, Microbial Cell Factories, Vol 20, Iss 1, Pp 1-14 (2021)
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Background The objective of this work was to engineer Deinococcus radiodurans R1 as a microbial cell factory for the production of pinene, a monoterpene molecule prominently used for the production of fragrances, pharmaceutical products, and jet engine biofuels. Our objective was to produce pinene from glycerol, an abundant by-product of various industries. Results To enable pinene production in D. radiodurans, we expressed the pinene synthase from Abies grandis, the geranyl pyrophosphate (GPP) synthase from Escherichia coli, and overexpressed the native 1-deoxy-d-xylulose 5-phosphate synthase. Further, we disrupted the deinoxanthin pathway competing for the substrate GPP by either inactivating the gene dr0862, encoding phytoene synthase, or substituting the native GPP synthase with that of E. coli. These manipulations resulted in a D. radiodurans strain capable of producing 3.2 ± 0.2 mg/L pinene in a minimal medium supplemented with glycerol, with a yield of 0.13 ± 0.04 mg/g glycerol in shake flask cultures. Additionally, our results indicated a higher tolerance of D. radiodurans towards pinene as compared to E. coli. Conclusions In this study, we successfully engineered the extremophile bacterium D. radiodurans to produce pinene. This is the first study demonstrating the use of D. radiodurans as a cell factory for the production of terpenoid molecules. Besides, its high resistance to pinene makes D. radiodurans a suitable host for further engineering efforts to increase pinene titer as well as a candidate for the production of the other terpenoid molecules.
- Subjects :
- Glycerol
Monoterpene
Pinene
Bioengineering
medicine.disease_cause
Microbiology
Applied Microbiology and Biotechnology
Metabolic engineering
chemistry.chemical_compound
Biofuel
Escherichia coli
medicine
Phytoene synthase
biology
Research
Geranyl pyrophosphate
Deinococcus radiodurans
Deinococcus radiodurans R1
biology.organism_classification
QR1-502
Terpenoid
Metabolic Engineering
chemistry
Biochemistry
Biofuels
Monoterpenes
biology.protein
Deinococcus
Abies
Biotechnology
Subjects
Details
- ISSN :
- 14752859
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Microbial Cell Factories
- Accession number :
- edsair.doi.dedup.....7da31a765d83e5f0f57545c82374fe9e