201. Distribution of vibrio cholerae non-ol in and around the Hiroshima City aquatic environment, Japan
- Author
-
Rajam Rajendran, Hiroyuki Nakano, Ciira Kiiyukia, Hideo Hashimoto, and Hideyuki Kawakami
- Subjects
Sediment ,Hemolysin ,Enterotoxin ,Biology ,medicine.disease_cause ,biology.organism_classification ,Applied Microbiology and Biotechnology ,Microbiology ,Salinity ,Vibrio cholerae non-O1 ,Vibrio cholerae ,Vibrionaceae ,medicine ,Bacteria - Abstract
A survey of Vibrio cholerae non-O1 from surface water and sediment samples was carried out in the aquatic environment of Hiroshima City. Of 72 water samples, 49 (68.1%) were positive for the organism, while in sediment samples 17 of 47 (36.2%) were positive. The incidence of the organism was higher in summer than in other seasons in both water and sediment samples. Isolation frequency of V. cholerae non-O1 was independent of other marine vibrios. Salinity and pH did not have an obvious influence on the distribution of this organism.All the isolates were hemolytic on sheep blood agar, and quantitative analysis revealed a high potential of hemolysin production of more than 1, 000 hemolytic units in 10 of 130 strains tested. A large number of isolates (60/102) were cytotoxigenic on CHO cell culture. Various cytopathic effects were displayed, including cytolytic, cell elongation, and a combination of these. All the isolates were incapable of producing any detectable cholera-like enterotoxin. These findings suggest that V. cholerae non-O1 is widespread in and around the Hiroshima City aquatic environment throughout the year and possesses the ability to cause infection.
- Published
- 1991