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The capsule polysaccharide structure and biogenesis for non-O1 Vibrio cholerae NRT36S: genes are embedded in the LPS region
- Source :
- BMC Microbiology, BMC Microbiology, Vol 7, Iss 1, p 20 (2007)
- Publication Year :
- 2007
- Publisher :
- Maryland Shared Open Access Repository, 2007.
-
Abstract
- Background In V. cholerae, the biogenesis of capsule polysaccharide is poorly understood. The elucidation of capsule structure and biogenesis is critical to understanding the evolution of surface polysaccharide and the internal relationship between the capsule and LPS in this species. V. cholerae serogroup O31 NRT36S, a human pathogen that produces a heat-stable enterotoxin (NAG-ST), is encapsulated. Here, we report the covalent structure and studies of the biogenesis of the capsule in V. cholerae NRT36S. Results The structure of the capsular (CPS) polysaccharide was determined by high resolution NMR spectroscopy and shown to be a complex structure with four residues in the repeating subunit. The gene cluster of capsule biogenesis was identified by transposon mutagenesis combined with whole genome sequencing data (GenBank accession DQ915177). The capsule gene cluster shared the same genetic locus as that of the O-antigen of lipopolysaccharide (LPS) biogenesis gene cluster. Other than V. cholerae O139, this is the first V. cholerae CPS for which a structure has been fully elucidated and the genetic locus responsible for biosynthesis identified. Conclusion The co-location of CPS and LPS biosynthesis genes was unexpected, and would provide a mechanism for simultaneous emergence of new O and K antigens in a single strain. This, in turn, may be a key element for V. cholerae to evolve new strains that can escape immunologic detection by host populations.
- Subjects :
- Lipopolysaccharides
Microbiology (medical)
V. cholerae
Chromatography, Gas
Protein subunit
capsular (CPS) polysaccharide
Immunoblotting
Molecular Sequence Data
lcsh:QR1-502
Mutagenesis (molecular biology technique)
LPS biosynthesis genes
Biology
medicine.disease_cause
Microbiology
lcsh:Microbiology
03 medical and health sciences
Gene cluster
medicine
Amino Acid Sequence
Nuclear Magnetic Resonance, Biomolecular
Peptide sequence
Gene
Bacterial Capsules
030304 developmental biology
Vibrio cholerae non-O1
0303 health sciences
surface polysaccharide
Base Sequence
030306 microbiology
Glycosyltransferases
O Antigens
biogenesis
Microscopy, Electron
Mutagenesis
Vibrio cholerae
Chromatography, Gel
DNA Transposable Elements
Transposon mutagenesis
Genome, Bacterial
Biogenesis
Research Article
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- BMC Microbiology, BMC Microbiology, Vol 7, Iss 1, p 20 (2007)
- Accession number :
- edsair.doi.dedup.....8df5b231f42751457835193eb4e80fd7
- Full Text :
- https://doi.org/10.13016/m2bp92