247 results on '"Li Yanxiao"'
Search Results
202. Rapid detection of cadmium ions in meat by a multi-walled carbon nanotubes enhanced metal-organic framework modified electrochemical sensor.
- Author
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Wang, Xin, Xu, Yiwei, Li, Yahui, Li, Yanxiao, Li, Zhihua, Zhang, Wen, Zou, Xiaobo, Shi, Jiyong, Huang, Xiaowei, Liu, Chao, and Li, Wenting
- Subjects
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MULTIWALLED carbon nanotubes , *METAL-organic frameworks , *ELECTROCHEMICAL sensors , *CADMIUM , *HEAVY metals , *MEAT , *DETECTION limit - Abstract
• 1. The sensor based on UiO-66-NH 2 @MWCNTs was fabricated for Cd2+ detection. • 2. The sensor shows excellent electrochemical performance. • 3. The sensor has good sensitivity toward Cd2+ with a LOD of 0.2 μg/L. • 4. The sensor was reliable for determination of Cd2+ in real samples. This work presents an electrochemical device based on a composite modification of amine functionalized Zr(IV) metal–organic framework (UiO-66-NH 2) and multi-walled carbon nanotubes (MWCNTs) for voltammetry determination of cadmium ions (Cd2+). The UiO-66-NH 2 @MWCNTs composites were prepared by one-pot hydrothermal reaction. The prepared sensor performs excellent performance, which was attributed to the synergism between UiO-66-NH 2 with a special octahedral structure and enlarged surface area and MWCNTs with outstanding conductivity. Under optimal experiment condition, the fabricated sensor showed good linear relationship from 0.5 to 170 μg/L, with a detection limit of 0.2 μg/L. Finally, the sensor was successfully applied to detect Cd2+ in meat samples (N = 21) with relative standard deviation (RSD) lower than 4.5% and recovery of 95.1–107.5%, and the results were compared with certified method, there was no statistical significance difference between the developed sensor and certified method at a 95% confidence level. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
203. A visual bi-layer indicator based on roselle anthocyanins with high hydrophobic property for monitoring griskin freshness.
- Author
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Zhang, Junjun, Huang, Xiaowei, Shi, Jiyong, Liu, Li, Zhang, Xinai, Zou, Xiaobo, Xiao, Jianbo, Zhai, Xiaodong, Zhang, Di, Li, Yanxiao, and Shen, Tingting
- Subjects
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ROSELLE , *FOOD packaging , *POLYVINYLIDENE fluoride , *POLYVINYL alcohol , *HYDROPHOBIC surfaces , *ANTHOCYANINS , *SODIUM alginate - Abstract
[Display omitted] • The PVDF film was first used as a moisture prevent-layer of freshness indicator. • The PSRF film showed highest hydrophobic property and color stability. • The PSRF film has been successfully used as a visual indicator for monitoring griskin freshness. This study designed a new type indicator with hydrophobic Polyvinylidene Fluoride (PVDF) film as a moisture prevent-layer. And the sensor layer was provided based on polyvinyl alcohol/Sodium alginate (PS) and Roselle anthocyanins (RAs). Physical properties, microstructure, and color stability of the bi-layer indicator have been investigated. The Water contact angle (WCA) of PS/RAs/ PVDF film (PSRF) was 108.85°, which can be considered as an excellent hydrophobic surface. The lowest Water Vapor Permeability (WVP) value of PSRF exhibited a good barrier property for moisture. Therefore, PSRF film was used to monitor the griskin freshness. The Total volatile basic nitrogen (TVB-N) level was increased to 18.02 mg/100 g at 72 h, and the color of the indicator presented visible color changes. The acquired results revealed a good correlation between TVB-N, pH and color change of the indicator. The research indicated that PSRF indicator has increasing potential application on food intelligent packaging. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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204. A nitrile-mediated SERS aptasensor coupled with magnetic separation for optical interference-free detection of atrazine.
- Author
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Wei, Xiaoou, Sun, Yue, Liu, Chao, Li, Zhihua, Zou, Xiaobo, Zhang, Di, Zhang, Wen, Shi, Jiyong, Huang, Xiaowei, and Li, Yanxiao
- Subjects
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ATRAZINE , *MAGNETIC separation , *MAGNETIC coupling , *COMPLEMENTARY DNA , *RAMAN scattering , *OPTICAL interference - Abstract
• A sensitive and selective SERS aptasensor was developed for atrazine detection. • A nitrile-mediated molecule (MMBN) was utilized as optical interference-free Raman tag. • Fe 3 O 4 @Au core/shell nanoparticles were used as SERS substrate for rapid enrichment. • Reliable measurements of atrazine were well established in real samples. A novel aptasensor based on the combination of surface-enhanced Raman scattering (SERS) and magnetic separation is presented for sensitive and selective determination of atrazine. 4-(Mercaptomethyl) benzonitrile (MMBN), providing a prominent peak (2227 cm−1) in the Raman-silent spectral window (1800–2800 cm−1), is selected as Raman tag to eliminate optical interference from biomolecules in sensing system. The MMBN and atrazine-binding aptamer are assembled on silver nanoparticles (AgNPs) to serve as Raman probe (MMBN-AgNPs-aptamer). Meanwhile, Fe 3 O 4 @Au core/shell nanoparticles conjugated with the complementary DNA (Fe 3 O 4 @Au-cDNA) are prepared as Raman substrate to extract the Raman probe with magnetic separation. Atrazine specifically binds to aptamer, and hinders the formation of Fe 3 O 4 @Au-cDNA-aptamer-AgNPs-MMBN (expressed as "Fe 3 O 4 @Au-AgNPs") complex. Therefore, the Raman intensity of MMBN on substrate decreases with the increment of atrazine concentration. Under optimal SERS conditions, the proposed aptasensor is used to detect atrazine in the laboratory and real samples. The as-prepared atrazine sensor demonstrates a broad dynamic range of 1–50 nM, as well as a low detection limit of 0.67 nM (S/N = 3). For real sample test, the recovery rate ranges from 98.7%–106.6%. The fabricated aptasensor shows superior interference-free performance for atrazine determination in the presence of analogs at high levels. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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205. A dual-emission fluorescence sensor for ultrasensitive sensing mercury in milk based on carbon quantum dots modified with europium (III) complexes.
- Author
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Gan, Ziyu, Hu, Xuetao, Huang, Xiaowei, Li, Zhihua, Zou, Xiaobo, Shi, Jiyong, Zhang, Wen, Li, Yanxiao, and Xu, Yiwei
- Subjects
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MERCURY , *DUAL fluorescence , *QUANTUM dots , *EUROPIUM , *RARE earth ions , *CARBON emissions , *PHOSPHORS - Abstract
• Dual-emission carbon quantum dots modified with europium complexes was prepared. • The sensor exhibits ultra-high sensitivity for Hg2+ detection. • The low detection limit was 0.2 nM. • The fluorescent change of the sensor could be visually observed by naked eyes. A dual-emission fluorescence sensor was developed for Hg2+detection based on carbon quantum dots (CQDs) and Europium (III, Eu3+) complexes. The CQDs modified with Eu3+ complexes displayed dual-emission wavelengths of 443 and 617 nm. The dual-emission fluorescence intensity was significantly increased in the presence of Hg2+. The developed sensor displayed excellent selectivity and sensitivity with the detection range of 1–20 nM and detection limit of 0.2 nM. With increasing Hg2+ concentrations, developed sensor showed clear and regular color alternations from strong blue, light blue, lavender to deep purple observed by the naked eyes. The dual-emission method was successfully used for visual and ultrasensitive detection of Hg2+ in drinking water and milk samples with satisfied recovery from 97.6 % to 105.4 %. The sensor provides a generalizable platform for dual-emission fluorescence detection of Hg2+ based on rare earth ions-modified CQDs. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
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206. The Function and Mechanism of Laminaripentaose Prepared from Curdlan for the Amelioration of the Cognitive Dysfunctions in Obese Mice.
- Author
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Xu Y, Li Y, Yan Q, Mao X, Yang S, and Jiang Z
- Subjects
- Animals, Mice, Male, Humans, Cognition drug effects, Bacteria classification, Bacteria drug effects, Bacteria isolation & purification, Diet, High-Fat adverse effects, Mice, Obese, Hippocampus drug effects, Hippocampus metabolism, Mice, Inbred C57BL, Obesity metabolism, Obesity drug therapy, Obesity physiopathology, Gastrointestinal Microbiome drug effects, Cognitive Dysfunction drug therapy, Cognitive Dysfunction etiology, Cognitive Dysfunction prevention & control, beta-Glucans pharmacology, Oligosaccharides administration & dosage
- Abstract
Functional oligosaccharides induce specific alterations in gut microbiota, potentially providing physiological benefits. However, the effects of laminaripentaose (LPA) on metabolic syndrome and the mechanism underlying it have not been intensively investigated yet. This study aimed to determine the effects of LPA on obesity and obesity-induced cognition impairment in mice. C57BL/6N mice fed with a high-fat diet received an LPA treatment for 12 weeks. An antibiotic intervention was further applied to evaluate the effects of the gut microbiota on cognitive functions. LPA treatment (500 mg/kg) reduced the weight gain by 32.4%. Furthermore, LPA improved memory functions and reduced hippocampal insulin resistance and neuronal injury. LPA markedly reduced systemic low-grade inflammation and intestinal barrier injury. Moreover, LPA increased gut beneficial bacteria, and Butyricimonas and Bifidobacterium were increased by 94.0 and 422.7%, respectively, accompanied by increased fecal short-chain fatty acids. Interestingly, antibiotic cocktail treatment abrogated the beneficial effects of LPA on cognition, which further suggests that LPA may attenuate obesity-induced cognition impairment via the gut-brain axis. Our findings provide the first evidence for the potential of dietary LPA to prevent obesity and obesity-associated complications.
- Published
- 2024
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207. Superoxide radicals mediated by high-spin Fe catalysis for organic wastewater treatment.
- Author
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Li Y, Zhang D, Wang P, Qu J, and Zhan S
- Abstract
Water resources are indispensable basic resources and important environmental carriers; the presence of organic contaminants in wastewater poses considerable risks to the health of both humans and ecosystems. Although the Fenton-like reactions using H
2 O2 as the oxidant to destroy organic pollutants are attractive, there are still challenges in improving reaction activity under neutral or even alkaline conditions. Herein, we designed a H2 O2 activation pathway with O2 •- as the main active species and elucidated that the spin interaction between Fe sites and coordinated O atoms effectively promotes the generation of the key intermediate Fe-*OOH. Furthermore, we successfully captured and analyzed the Fe-*OOH intermediate by in situ Raman spectroscopy. When applying FBOB to a continuous-flow reactor, CIP removal efficiency remained at around 90% within 600 min of continuous operation, achieving excellent efficiency, stability, and pH tolerance in removing pollutants., Competing Interests: Competing interests statement:The authors declare no competing interest.- Published
- 2024
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208. Identification and characterization of the RcTCP gene family and its expression in response to abiotic stresses in castor bean.
- Author
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Li Y, Liu X, Xu X, Zhu G, Xiang D, and Liu P
- Subjects
- Promoter Regions, Genetic, Transcription Factors genetics, Transcription Factors metabolism, MicroRNAs genetics, MicroRNAs metabolism, Gene Expression Profiling, Stress, Physiological genetics, Ricinus communis genetics, Plant Proteins genetics, Plant Proteins metabolism, Gene Expression Regulation, Plant, Phylogeny, Multigene Family
- Abstract
Background: The TCP (teosinte branched1/cincinnata/proliferating cell factor) family plays a prominent role in plant development and stress responses. However, TCP family genes have thus far not been identified in castor bean, and therefore an understanding of the expression and functional aspects of castor bean TCP genes is lacking. To identify the potential biological functions of castor bean (RcTCP) TCP members, the composition of RcTCP family members, their basic physicochemical properties, subcellular localizations, interacting proteins, miRNA target sites, and gene expression patterns under stress were assessed., Results: The presence of 20 RcTCP genes on the nine chromosomes of castor bean was identified, all of which possess TCP domains. Phylogenetic analysis indicated a close relationship between RcTCP genes and Arabidopsis AtTCP genes, suggesting potential functional similarity. Subcellular localization experiments confirmed that RcTC01/02/03/10/16/18 are all localized in the nucleus. Protein interaction analysis revealed that the interaction quantity of RcTCP03/06/11 proteins is the highest, indicating a cascade response in the functional genes. Furthermore, it was found that the promoter region of RcTCP genes contains a large number of stress-responsive elements and hormone-induced elements, indicating a potential link between RcTCP genes and stress response functions. qRT-PCR showed that all RcTCP genes exhibit a distinct tissue-specific expression pattern and their expression is induced by abiotic stress (including low temperature, abscisic acid, drought, and high salt). Among them, RcTCP01/03/04/08/09/10/14/15/18/19 genes may be excellent stress-responsive genes., Conclusion: We discovered that RcTCP genes play a crucial role in various activities, including growth and development, the stress response, and transcription. This study provides a basis for studying the function of RcTCP gene in castor., (© 2024. The Author(s).)
- Published
- 2024
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209. Species-specific duplicated FMRFaR-like gene A62 regulates spontaneous locomotion in Apolygus lucorum.
- Author
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Gao H, Tian Y, Zhang H, Li Y, Li C, and Li B
- Subjects
- Animals, Heteroptera genetics, Heteroptera physiology, Heteroptera growth & development, Receptors, G-Protein-Coupled genetics, Receptors, G-Protein-Coupled metabolism, Species Specificity, Gene Duplication, Locomotion, Insect Proteins genetics, Insect Proteins metabolism
- Abstract
Background: Apolygus lucorum, a major cotton pest, has undergone a significant expansion of the FMRFaR gene within the GPCR superfamily, resulting in two classes of GPCR, namely FMRFaR (A54-55) and newly duplicated FMRFaR-like (A56-62). Notably, FMRFaR-like genes, particularly A62, show enhanced expression in the legs and wings of adults, indicating their potential role in locomotion. Employing A62 as a representative of FMRFaR-like, our study investigates the influence of FMRFa, FMRFaR, and FMRFaR-like on locomotion and development of A. lucorum., Results: FMRFaR and FMRFa exhibit comparable temporal and tissue expression patterns, whereas the FMRFaR-like genes within A. lucorum exhibit completely distinct evolutionary and expression patterns compared to classical FMRFaR. RNA interference (RNAi) experiments revealed that suppressing FMRFa expression results in complete lethality in A. lucorum, but neither FMRFaR nor A62 exhibit the same effect after RNAi. Suppressing the expression of FMRFa only decreases the expression of the A54 gene simultaneously, suggesting that A54 may function as a classical FMRFaR activated by FMRFa. RNAi of A62 leads to wing malformation and a significant reduction in spontaneous movement behavior in A. lucorum. Further transcriptomic analysis revealed that A62 affects the A. lucorum's movement behavior through energy metabolism pathways and motor protein pathways., Conclusion: Our study unveils the unique and complex roles of FMRFa and its receptor in A. lucorum. These findings provide valuable insights into potential targets for pest control strategies aimed at managing A. lucorum populations in cotton fields. © 2024 Society of Chemical Industry., (© 2024 Society of Chemical Industry.)
- Published
- 2024
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210. Genome-wide analysis of LOG family genes in castor and RcLOG5 enhances drought, salt, and cold stress tolerance in Arabidopsis thaliana.
- Author
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Li Y, Zhu G, Sun H, Xiang D, Zhang C, Li Z, and Liu P
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- Cold-Shock Response genetics, Droughts, Stress, Physiological genetics, Gene Expression Regulation, Plant, Plant Proteins genetics, Plant Proteins metabolism, Plant Breeding, Plants, Genetically Modified genetics, Plants, Genetically Modified metabolism, Glutathione genetics, Arabidopsis metabolism
- Abstract
The gene encoding the specific phosphohydrolase LONELY GUY (LOG) plays an important role in the activation of cytokinin and the stress response in plant cells. However, the role of LOG genes in castor bean (Ricinus communis) has not been reported. In this study, we identified a total of nine members of the LOG gene family in the castor bean genome and investigated the upregulated expression of the RcLOG5 gene using transcriptome data analysis. We found that the RcLOG5 gene exhibited tissue-specific expression and was activated by polyethylene glycol, NaCl, low temperature, and abscisic acid stress. The subcellular localization results showed that the RcLOG5 gene is mainly located in the cytoplasm. Based on phenotypic and physiological indicators, namely root length, peroxidase activity, and malondialdehyde content, overexpression of the RcLOG5 gene not only improved the drought resistance, salt tolerance, and cold tolerance of transgenic Arabidopsis, but also shortened the dormancy period of the transgenic plants. Transcriptomic sequencing revealed that the overexpression of the RcLOG5 gene led to the enrichment of differentially expressed genes in the glutathione metabolism pathway in transgenic Arabidopsis. Moreover, the overexpression plants had higher levels of glutathione and a higher GSH/GSSG ratio under stress compared to the wild type. Therefore, we inferred that the RcLOG5 gene may be responsible for regulating cell membrane homeostasis by reducing the accumulation of reactive oxygen species through the glutathione pathway. Overall, the overexpression of the RcLOG5 gene positively regulated the stress resistance of transgenic Arabidopsis. This study provides valuable gene resources for breeding stress-tolerant castor bean varieties., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
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211. Single-cell landscape reveals the epithelial cell-centric pro-inflammatory immune microenvironment in dry eye development.
- Author
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Liu Z, Xie H, Li L, Jiang D, Qian Y, Zhu X, Dai M, Li Y, Wei R, Luo Z, Xu W, Zheng Q, Shen J, Zhou M, Zeng W, and Chen W
- Subjects
- Animals, Mice, Humans, Gene Expression Profiling, Inflammation immunology, Transcriptome, T-Lymphocyte Subsets immunology, T-Lymphocyte Subsets metabolism, Mice, Inbred C57BL, Dry Eye Syndromes immunology, Dry Eye Syndromes etiology, Single-Cell Analysis, Epithelial Cells immunology, Macrophages immunology, Conjunctiva immunology, Conjunctiva pathology, Cellular Microenvironment, Disease Models, Animal
- Abstract
Dry eye disease (DED) is a prevalent chronic eye disease characterized by an aberrant inflammatory response in ocular surface mucosa. The immunological alterations underlying DED remain largely unknown. In this study, we employed single-cell transcriptome sequencing of conjunctival tissue from environment-induced DED mice to investigate multicellular ecosystem and functional changes at different DED stages. Our results revealed an epithelial subtype with fibroblastic characteristics and pro-inflammatory effects emerging in the acute phase of DED. We also found that T helper (Th)1, Th17, and regulatory T cells (Treg) were the dominant clusters of differentiation (CD)4
+ T-cell types involved in regulating immune responses and identified three distinct macrophage subtypes, with the CD72+ CD11c+ subtype enhancing chronic inflammation. Furthermore, bulk transcriptome analysis of video display terminal-induced DED consistently suggested the presence of the pro-inflammatory epithelial subtype in human conjunctiva. Our findings have uncovered a DED-associated pro-inflammatory microenvironment in the conjunctiva, centered around epithelial cells, involving interactions with macrophages and CD4+ T cells, which deepens our understanding of ocular surface mucosal immune responses during DED progression., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)- Published
- 2024
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212. Molecular level removal of antibiotic resistant bacteria and genes: A review of interfacial chemical in advanced oxidation processes.
- Author
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Li F, Liu K, Bao Y, Li Y, Zhao Z, Wang P, and Zhan S
- Subjects
- Wastewater, Ecosystem, Angiotensin Receptor Antagonists pharmacology, Anti-Bacterial Agents pharmacology, Angiotensin-Converting Enzyme Inhibitors pharmacology, Genes, Bacterial, Bacteria genetics
- Abstract
As a kind of novel and persistent environmental pollutants, antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) have been frequently detected in different aquatic environment, posing potential risks to public health and ecosystems, resulting in a biosecurity issue that cannot be ignored. Therefore, in order to control the spread of antibiotic resistance in the environment, advanced oxidation technology (such as Fenton-like, photocatalysis, electrocatalysis) has become an effective weapon for inactivating and eliminating ARB and ARGs. However, in the process of advanced oxidation technology, studying and regulating catalytic active sites at the molecular level and studying the adsorption and surface oxidation reactions between catalysts and ARGs can achieve in-depth exploration of the mechanism of ARGs removal. This review systematically reveals the catalytic sites and related mechanisms of catalytic antagonistic genes in different advanced oxidation processes (AOPs) systems. We also summarize the removal mechanism of ARGs and how to reduce the spread of ARGs in the environment through combining a variety of characterization methods. Importantly, the potential of various catalysts for removing ARGs in practical applications has also been recognized, providing a promising approach for the deep purification of wastewater treatment plants., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
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213. Structural and functional analysis of SpGlu64A: a novel glycoside hydrolase family 64 laminaripentaose-producing β-1,3-glucanase from Streptomyces pratensis.
- Author
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Ma J, Jiang Z, Yan Q, Lv A, Li Y, and Yang S
- Subjects
- Substrate Specificity, Bacterial Proteins genetics, Bacterial Proteins metabolism, Bacterial Proteins chemistry, Models, Molecular, Glucan 1,3-beta-Glucosidase metabolism, Glucan 1,3-beta-Glucosidase genetics, Glucan 1,3-beta-Glucosidase chemistry, Amino Acid Sequence, Glycoside Hydrolases genetics, Glycoside Hydrolases metabolism, Glycoside Hydrolases chemistry, Catalytic Domain, Crystallography, X-Ray, Hydrolysis, Hydrogen-Ion Concentration, Kinetics, Streptomyces enzymology, Streptomyces genetics, beta-Glucans metabolism, Oligosaccharides metabolism, Oligosaccharides chemistry
- Abstract
Laminaripentaose (L5)-producing β-1,3-glucanases can preferentially cleave the triple-helix curdlan into β-1,3-glucooligosaccharides, especially L5. In this study, a newly identified member of the glycoside hydrolase family 64, β-1,3-glucanase from Streptomyces pratensis (SpGlu64A), was functionally and structurally characterized. SpGlu64A shared highest identity (30%) with a β-1,3-glucanase from Streptomyces matensis. The purified SpGlu64A showed maximal activity at pH 7.5 and 50 °C, and exhibited strict substrate specificity toward curdlan (83.1 U·mg
-1 ). It efficiently hydrolyzed curdlan to produce L5 as the end product. The overall structure of SpGlu64A consisted of a barrel domain and a mixed (α/β) domain, which formed an unusually wide groove with a crescent-like structure. In the two complex structures (SpGlu64A-L3 and SpGlu64A-L4), two oligosaccharide chains were captured and the triple-helical structure was relatively compatible with the wide groove, which suggested the possibility of binding to the triple-helical β-1,3-glucan. A catalytic framework (β6-β9-β10) and the steric hindrance formed by the side chains of residues Y161, N163, and H393 in the catalytic groove were predicted to complete the exotype-like cleavage manner. On the basis of the structure, a fusion protein with the CBM56 domain (SpGlu64A-CBM) and a mutant (Y161F; by site-directed mutation) were obtained, with 1.2- and 1.7-fold increases in specific activity, respectively. Moreover, the combined expression of SpGlu64A-CBM and -Y161F improved the enzyme activity by 2.63-fold. The study will not only be helpful in understanding the reaction mechanism of β-1,3-glucanases but will also provide a basis for further enzyme engineering., (© 2024 Federation of European Biochemical Societies.)- Published
- 2024
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214. Sodium alginate/guar gum based nanocomposite film incorporating β-Cyclodextrin/persimmon pectin-stabilized baobab seed oil Pickering emulsion for mushroom preservation.
- Author
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Yang Z, Li M, Li Y, Huang X, Li Z, Zhai X, Shi J, Zou X, Xiao J, Sun Y, Povey M, Gong Y, and Holmes M
- Subjects
- Pectins, Alginates chemistry, Emulsions, Silver chemistry, Water, Plant Oils, Diospyros, Adansonia, Metal Nanoparticles chemistry, Agaricales, Nanocomposites chemistry
- Abstract
The poor biological, mechanical and water-resistance properties of sodium alginate/guar gum film (SG) limit its application in food preservation. To overcome this disadvantage, we added β-Cyclodextrin/persimmon pectin-stabilized baobab seed oil Pickering emulsion (BOPE) to enhance the mechanical and water resistance properties of SG film, and added green synthesized silver nanoparticles (AgNPS) and Lycium ruthenicum extract (LA) to improve the biological properties of the film. The properties of BOPE was optimized using Box-Behnken design (BBD). Scanning electron microscope and Fourier transform infrared results revealed the change of structure and molecular interaction in the SG film after the addition of AgNPS, LA, and optimized BOPE. The 2.0%BOPE-loaded film containing AgNPS/LA with the enhanced mechanical, barrier, BO retention, and biological properties not only improved the preservation effect on mushroom (A. bisporus), but also maintained structural stability. Thus, the 2.0%BOPE-loaded SG/LA/AgNPS film has considerable potential in active packaging applications., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)
- Published
- 2024
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215. Probing the Mechanical Properties of 2D Materials via Atomic-Force-Microscopy-Based Modulated Nanoindentation.
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Khan RM, Rejhon M, Li Y, Parashar N, Riedo E, Wixom RR, DelRio FW, and Dingreville R
- Abstract
As the field of low-dimensional materials (1D or 2D) grows and more complex and intriguing structures are continuing to be found, there is an emerging need for techniques to characterize the nanoscale mechanical properties of all kinds of 1D/2D materials, in particular in their most practical state: sitting on an underlying substrate. While traditional nanoindentation techniques cannot accurately determine the transverse Young's modulus at the necessary scale without large indentations depths and effects to and from the substrate, herein an atomic-force-microscopy-based modulated nanomechanical measurement technique with Angstrom-level resolution (MoNI/ÅI) is presented. This technique enables non-destructive measurements of the out-of-plane elasticity of ultra-thin materials with resolution sufficient to eliminate any contributions from the substrate. This method is used to elucidate the multi-layer stiffness dependence of graphene deposited via chemical vapor deposition and discover a peak transverse modulus in two-layer graphene. While MoNI/ÅI has been used toward great findings in the recent past, here all aspects of the implementation of the technique as well as the unique challenges in performing measurements at such small resolutions are encompassed., (© 2023 The Authors. Small Methods published by Wiley-VCH GmbH.)
- Published
- 2024
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216. A ratiometric fluorescence amine sensor based on carbon quantum dot-loaded electrospun polyvinylidene fluoride film for visual monitoring of food freshness.
- Author
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Song W, Zhai X, Shi J, Zou X, Xue Y, Sun Y, Sun W, Zhang J, Huang X, Li Z, Shen T, Li Y, Zhou C, Holmes M, Gong Y, and Povey M
- Abstract
A ratiometric fluorescence sensor based on dual-emission carbon quantum dots (CQD) was developed to real time monitor food spoilage. Two hydrophobic electrospun fluorescent films were developed using polyvinylidene fluoride (PVDF) as the film-forming polymer in combination with CQD as the fluorescent probe. The CQD/PVDF film and CQD@PVDF film enabled the analysis of TMA with limits of detection (LODs) of 1.04 μM and 2.1 μM, respectively, and they exhibited excellent stability at 4 °C. By these virtues, the CQD@PVDF film exhibited visible fluorescence color changes from yellow green to blue by real time and nondestructively sensing volatile amines generated from beef, pork and shrimp in a packaging system with high humidity. This strategy provided a simple but useful, non-destructive, robust, and platform to real time monitor food spoilage for intelligent food packaging., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Ltd.)
- Published
- 2024
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217. Unraveling the G protein-coupled receptor superfamily in aphids: Contractions and duplications linked to phloem feeding.
- Author
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Gao H, Li Y, Zhang X, Zhang H, Tian Y, and Li B
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- Animals, Phylogeny, Phloem, Feeding Behavior physiology, Receptors, G-Protein-Coupled genetics, Opsins genetics, Aphids genetics
- Abstract
The G Protein-Coupled Receptor (GPCR) superfamily is the largest and most diverse transmembrane receptor family, playing crucial roles in regulating various physiological processes. As one of the most destructive pests, aphids have been subject to previous studies, which revealed fewer GPCR superfamily members in Acyrthosiphon pisum and Aphis gossypii and the loss of multiple neuropeptide GPCRs. To elucidate the contraction patterns and evolutionary features of the aphid GPCR superfamily, we identified 97, 105, and 95 GPCR genes in Rhopalosiphum maidis, A. pisum, and A. gossypii, respectively. Comparative analysis and phylogenetic investigations with other hemipteran insects revealed a contracted GPCR superfamily in aphids. This contraction mainly occurred in biogenic amine receptors, GABA-B-R, and fz families, and several neuropeptide receptors such as ACPR, CrzR, and PTHR were completely lost. This phenomenon may be related to the parasitic nature of aphids. Additionally, several GPCRs associated with aphid feeding and water balance underwent duplication, including Lkr, NPFR, CCHa1-R, and DH-R, Type A LGRs, but the SK/CCKLR that inhibits feeding was completely lost, indicating changes in feeding genes that underpin the aphid's prolonged phloem feeding behavior. Furthermore, we observed fine-tuning in opsins, with reduced long-wavelength opsins and additional duplications of short-wavelength opsin, likely associated with daytime activity. Lastly, we found variations in the number of mthl genes in aphids. In conclusion, our investigation sheds light on the GPCR superfamily in aphids, revealing its association with diet lifestyle and laying the foundation for understanding and developing control strategies for the aphid GPCR superfamily., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Inc. All rights reserved.)
- Published
- 2024
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218. Functional characterisation of Fe (II) and 2OG-dependent dioxygenase TcALKBH4 in the red flour beetle, Tribolium castaneum.
- Author
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Yu R, Zhang W, Li Y, Tang J, Kim K, and Li B
- Subjects
- Female, Male, Animals, Epigenesis, Genetic, Larva genetics, RNA Interference, Mammals, Coleoptera, Tribolium genetics
- Abstract
Alpha-ketoglutarate-dependent dioxygenase ALKB homologue 4 (ALKBH4) is a member of the Fe (II) and 2-oxoglutarate-dependent ALKB homologue family that plays important roles in epigenetic regulation by alkyl lesions removal in mammals. However, the roles of ALKBH4 in insects are not clear. Here, TcALKBH4 was cloned and functionally characterised in Tribolium castaneum. Temporal expression revealed that TcALKBH4 was highly expressed in early embryos and early pupae. Spatial expression showed that TcALKBH4 was highly expressed in the adult testis, and followed by the ovary. RNA interference targeting TcALKBH4 at different developmental stages in T. castaneum led to apparent phenotypes including the failure of development in larvae, the reduction of food intake and the deficiency of fertility in adult. However, further dot blot analyses showed that TcALKBH4 RNAi does not seem to influence 6 mA levels in vivo. qRT-PCR was used to further explore the underlying molecular mechanisms; the result showed that TcALKBH4 mediates the development of larvae possibly through 20E signalling pathway, and the fertility of female and male adult might be regulated by the expression of vitellogenesis and JH signalling pathway, respectively. Altogether, these findings will provide new insights into the potential function of ALKBH4 in insects., (© 2023 Royal Entomological Society.)
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- 2023
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219. Gene family expansion analysis and identification of the histone family in Spodoptera frugiperda.
- Author
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Gao H, Li Y, Tian Y, Zhang H, Kim K, and Li B
- Subjects
- Animals, Spodoptera genetics, Histones
- Abstract
Spodoptera frugiperda, a major invasive pest, causes severe damage to various economically important crops. Previous comparative genomics studies have revealed a close association between the invasiveness of S. frugiperda and its genome. In recent years, a vast amount of genome from lepidopteran species has become available, offering an opportunity for a more detailed and comprehensive understanding of the biological characteristics of S. frugiperda. In this study, we conducted a comprehensive comparative genomics analysis of S. frugiperda using genome from 46 lepidopteran species. We found the highest number of gene family expansion events in S. frugiperda, indicating that gene family expansion is a crucial mechanism in its adaptive evolution. The expanded gene families are enriched in various biological processes, including nutrient metabolism, development, stress response, reproduction, and immune processes, suggesting that the expansion of these gene families likely contributes to the strong environmental adaptability of S. frugiperda. Furthermore, we identified the expansion of histone gene families in S. frugiperda which resulted from chromosome segmental duplications after the divergence from closely related species. Expression analysis of histone genes indicated that certain members might exert an influence on the growth and reproduction processes of S. frugiperda. Overall, our study deepens our understanding of the biological characteristics of S. frugiperda, providing a theoretical basis for the comprehensive management and sustained control of S. frugiperda and other lepidopteran pests in the future., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Inc.)
- Published
- 2023
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220. CRISPR/Cas9 System-Mediated Multi-copy Expression of an Alkaline Serine Protease in Aspergillus niger for the Production of XOD-Inhibitory Peptides.
- Author
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Wang S, Xue Y, Zhang P, Yan Q, Li Y, and Jiang Z
- Subjects
- Serine Proteases genetics, Serine Proteases metabolism, CRISPR-Cas Systems, Serine metabolism, Peptides genetics, Peptides metabolism, Serine Endopeptidases genetics, Serine Endopeptidases metabolism, Aspergillus niger genetics, Aspergillus niger metabolism, Xanthine Oxidase metabolism
- Abstract
CRISPR/Cas9 system-mediated multi-copy expression of an alkaline serine protease ( Aopro S8) from Aspergillus oryzae was successfully built in Aspergillus niger . Furthermore, AoproS8 was continuously knocked in the glaA , amyA , and aamy gene loci in A. niger to construct multi-copy expression strains. The yield of the AoproS8 3.0 strain was 2.1 times higher than that of the AoproS8 1.0 strain. Then, a high protease activity of 11,023.2 U/mL with a protein concentration of 10.8 mg/mL was obtained through fed-batch fermentation in a 5 L fermenter. This is the first report on the high-level expression of alkaline serine proteases in A. niger . AoproS8 showed optimal activity at pH 9.0 and 40 °C. It was used for the production of xanthine oxidase (XOD)-inhibitory peptides from eight food processing protein by-products. Among them, the duck hemoglobin hydrolysates showed the highest XOD-inhibitory activity with an IC
50 value of 2.39 mg/mL. Thus, our work provides a useful way for efficient expression of proteases in A. niger and high-value utilization of protein by-products.- Published
- 2023
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221. Entrapment of probiotic (Bifidobacterium longum) in bilayer emulsion film with enhanced barrier property for improving viability.
- Author
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Yang Z, Li M, Li Y, Wang X, Li Z, Shi J, Huang X, Zhai X, Zou X, Gong Y, Holmes M, Povey M, and Xiao J
- Subjects
- Bifidobacterium, Emulsions, Gelatin, Bifidobacterium longum, Probiotics chemistry
- Abstract
The gelatin/gellan gum based-bilayer emulsion film was developed in this work to improve the survivability of Bifidobacterium longum during the storage process. The baobab seed oil (BO) was added to the gelatin (GE) matrix to develop emulsion film as the barrier outer layer. The blueberry anthocyanin extract (BE) was incorporated into the gellan gum (GG)-based inner layer to enhance the viability of B. longum. The SEM and FTIR results revealed that the probiotics were successfully entrapped in BO/BE-loaded bilayer film. The greatest survivability and viable cell numbers of the B. longum during the storage period were observed in the BO/BE loaded bilayer film. Furthermore, the stability of the colorful patterns by electrochemical writing was also evaluated in this work. Finally, the GE/BO-GG/BE/BM maintain satisfactory probiotic viability in steamed bread coating application. Hence, the GE/BO-GG/BE/BM bilayer film could be considered a novel material to deliver and protect the probiotics in food applications., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)
- Published
- 2023
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222. Dietary neoagarotetraose extends lifespan and impedes brain aging in mice via regulation of microbiota-gut-brain axis.
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Li T, Yang S, Liu X, Li Y, Gu Z, and Jiang Z
- Subjects
- Mice, Animals, Longevity, Mice, Inbred C57BL, Aging, Brain metabolism, Brain pathology, Brain-Gut Axis, Alzheimer Disease drug therapy, Alzheimer Disease metabolism, Alzheimer Disease microbiology
- Abstract
Introduction: Dietary oligosaccharides can impact the gut microbiota and confer tremendous health benefits., Objectives: The aim of this study was to determine the impact of a novel functional oligosaccharide, neoagarotetraose (NAT), on aging in mice., Methods: 8-month-old C57BL/6J mice as the natural aging mice model were orally administered with NAT for 12 months. The preventive effect of NAT in Alzheimer's disease (AD) mice was further evaluated. Aging related indicators, neuropathology, gut microbiota and short-chain fatty acids (SCFAs) in cecal contents were analyzed., Results: NAT treatment extended the lifespan of these mice by up to 33.3 %. Furthermore, these mice showed the improved aging characteristics and decreased injuries in cerebral neurons. Dietary NAT significantly delayed DNA damage in the brain, and inhibited reduction of tight junction protein in the colon. A significant increase at gut bacterial genus level (such as Lactobacillus, Butyricimonas, and Akkermansia) accompanied by increasing concentrations of SCFAs in cecal contents was observed after NAT treatment. Functional profiling of gut microbiota composition indicated that NAT treatment regulated the glucolipid and bile acid-related metabolic pathways. Interestingly, NAT treatment ameliorated cognitive impairment, attenuated amyloid-β (Aβ) and Tau pathology, and regulated the gut microbiota composition and SCFAs receptor-related pathway of Alzheimer's disease (AD) mice., Conclusion: NAT mitigated age-associated cerebral injury in mice through gut-brain axis. The findings provide novel evidence for the effect of NAT on anti-aging, and highlight the potential application of NAT as an effective intervention against age-related diseases., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Production and hosting by Elsevier B.V.)
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- 2023
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223. A cell-based electrochemical taste sensor for detection of Hydroxy-α-sanshool.
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Hao M, Li Z, Huang X, Wang Y, Wei X, Zou X, Shi J, Huang Z, Yin L, Gao L, Li Y, Holmes M, and Elrasheid Tahir H
- Subjects
- Humans, Taste, Cysteine, Polyunsaturated Alkamides chemistry, Electrodes, Electrochemical Techniques, Limit of Detection, Biosensing Techniques, Zanthoxylum chemistry
- Abstract
The Transient Receptor Potential Vanilloid 1 (TRPV1) has been identified as a suitable candidate for a spicy taste (Zanthoxylum plant) sensor. In this study, we investigated the response of TRPV1 expressed on human HepG2 cell membranes following stimulation with Hydroxy-α-sanshool. A three-dimensional (3D) cell-based electrochemical sensor was fabricated by layering cells expressing hTRPV1. l-cysteine/AuNFs electrodes were functionalized on indium tin oxide-coated glass (ITO) to enhance the sensor's selectivity and sensitivity. HepG2 cells were encapsulated in sodium alginate/gelatin hydrogel to create a 3D cell cultivation system, which was immobilized on the l-cysteine/AuNFs/ITO to serve as biorecognition elements. Using differential pulse voltammetry (DPV), the developed biosensor was utilized to detect Hydroxy-α-sanshool, a representative substance in Zanthoxylum bungeanum Maxim. The result obtained from DPV was linear with Hydroxy-α-sanshool concentrations ranging from 0 to 70 μmol/L, with a detection limit of 2.23 μmol/L. This biosensor provides a sensitive and novel macroscopic approach for TRPV1 detection., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)
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- 2023
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224. [Identification and expression pattern analysis of RcACA gene family in castor under abiotic stresses].
- Author
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Li Y, Zhang C, Geng L, Chen Y, Zhang L, Xiang D, and Liu P
- Subjects
- Transcriptome, Promoter Regions, Genetic, Phylogeny, Plant Proteins genetics, Plant Proteins metabolism, Gene Expression Regulation, Plant, Genome, Plant, Stress, Physiological genetics
- Abstract
Auto-inhibited Ca
2+ -ATPase (ACA) is one of the Ca2+ -ATPase subfamilies that plays an important role in maintaining Ca2+ concentration balance in plant cells. To explore the function and gene expression pattern of the RcACA gene family in castor, bioinformatics analysis was used to identify the members of the RcACA gene family in castor. The basic physical and chemical properties, subcellular location, protein secondary and tertiary structure, conserved domain, conserved motif, gene structure, chromosome location and collinear relationship, as well as the evolutionary characteristics and promoter cis -acting elements were predicted and analyzed. The expression pattern of the RcACA gene under abiotic stress was analyzed by expression (fragments per kilobase of exon model per million mapped fragments, FPKM) in castor transcriptome data. The results showed that 8 RcACA gene family members were identified in castor, acidic proteins located in the plasma membrane. In the secondary structure of all proteins, the α-helix and random coil is more; the RcACA genes were clustered into three categories, and the design of the genes in the same category was similar to the conserved motif. Both of them had four typical domains, RcACA3 - RcACA8 had a Ca2+ -ATPase N-terminal autoinhibitory domain. The RcACA gene is mostly located on the long arm of the chromosome and has 2 pairs of collinear relationships. There are more light response elements but fewer hormone-induced elements located upstream of the RcACA coding region. Interspecific clustering showed that the evolution of ACA genes among species was conservative. Tissue expression pattern analysis showed that RcACA genes showed apparent tissue expression specificity, and most of the genes showed the highest expression level in male flowers. Expression analysis under abiotic stress showed that RcACA2 - RcACA8 were up-regulated under high salt and drought stress, and RcACA1 was up-regulated at 0-24 h under low-temperature stress, indicating that RcACA genes positively responded to abiotic stresses. The above results provide a theoretical basis for exploring the role of the RcACA gene in castor growth, development and stress response.- Published
- 2023
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225. Dynamic active-site induced by host-guest interactions boost the Fenton-like reaction for organic wastewater treatment.
- Author
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Zhang D, Li Y, Wang P, Qu J, Li Y, and Zhan S
- Subjects
- Hydrogen Peroxide chemistry, Catalytic Domain, Oxidation-Reduction, Oxygen, Iron chemistry, Water Purification methods
- Abstract
In heterogeneous catalysis, uncovering the dynamic evolution of active sites in the working conditions is crucial to realizing increased activity and enhanced stability of catalyst in Fenton-like activation. Herein, we capture the dynamic changes in the unit cell of Co/La-SrTiO
3 catalyst during the exemplary peroxymonosulfate activation process using X-ray absorption spectroscopy and in situ Raman spectroscopy, revealing the substrate tuned its structural evolution, which is the reversible stretching vibration of O-Sr-O and Co/Ti-O bonds in different orientations. This process effectively promotes the generation of key SO5 * intermediates, which is beneficial to the formation of1 O2 and SO4 •- from persulfate on the Co active site. Density functional theory and X-ray absorption spectroscopy show that the optimized structural distortion enhanced the metal-oxygen bond strength by tuning the eg orbitals and increased the number of transferred electrons to peroxymonosulfate by about 3-fold, achieving excellent efficiency and stability in removing organic pollutants., (© 2023. The Author(s).)- Published
- 2023
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226. Regulating Spin Polarization through Cationic Vacancy Defects in Bi 4 Ti 3 O 12 for Enhanced Molecular Oxygen Activation.
- Author
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Zhang D, Li Y, Wang P, Qu J, Zhan S, and Li Y
- Abstract
Molecular oxygen (O
2 ) activation technology is of great significance in environmental purification due to its eco-friendly operation and cost-effective nature. However, the activation of O2 is limited by spin-forbidden transitions, and efficient molecular oxygen activation depends on electronic behavior and surface adsorption. Herein, we prepared cationic defect-rich Bi4 Ti3 O12 (BTO-MV2) catalysts containing Ti vacancies (VTi ) for O2 activation in water purification. The VTi on BTO nanosheets can induce electron spin polarization, increasing the number of spin-down photogenerated electrons and reducing the recombination of electron-hole pairs. An active surface VTi is also formed, serving as a center for adsorbing O2 and extracting electrons, effectively generating ⋅OH, O2 ⋅- and1 O2 . The degradation rate constant of tetracycline achieved by BTO-MV2 is 3.3 times faster than BTO, indicating a satisfactory prospect for practical application. This work provides an efficient pathway to activate molecular oxygen by constructing new active sites through cationic vacancy modification technology., (© 2023 Wiley-VCH GmbH.)- Published
- 2023
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227. Comparative study of neuropeptide signaling systems in Hemiptera.
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Gao H, Li Y, Zhang H, Wang S, Feng F, Tang J, and Li B
- Subjects
- Animals, Phylogeny, Peptides, Signal Transduction, Insecta metabolism, Insect Proteins metabolism, Neuropeptides genetics, Neuropeptides metabolism, Heteroptera
- Abstract
Numerous physiological processes in insects are tightly regulated by neuropeptides and their receptors. Although they form an ancient signaling system, there is still a great deal of variety in neuropeptides and their receptors among different species within the same order. Neuropeptides and their receptors have been documented in many hemipteran insects, but the differences among them have been poorly characterized. Commercial grapevines worldwide are plagued by the bug Daktulosphaira vitifoliae (Hemiptera: Sternorrhyncha). Here, 33 neuropeptide precursors and 48 putative neuropeptide G protein-coupled receptor (GPCR) genes were identified in D. vitifoliae. Their expression profiles at the probe and feeding stages reflected potential regulatory roles in probe behavior. By comparison, we found that the Releasing Hormone-Related Peptides (GnRHs) system of Sternorrhyncha was differentiated from those of the other 2 suborders in Hemiptera. Independent secondary losses of the adipokinetic hormone/corazonin-related peptide receptor (ACP) and corazonin (CRZ) occurred during the evolution of Sternorrhyncha. Additionally, we discovered that the neuropeptide signaling systems of Sternorrhyncha were very different from those of Heteroptera and Auchenorrhyncha, which was consistent with Sternorrhyncha's phylogenetic position at the base of the order. This research provides more knowledge on neuropeptide systems and sets the groundwork for the creation of novel D. vitifoliae management strategies that specifically target these signaling pathways., (© 2022 Institute of Zoology, Chinese Academy of Sciences.)
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- 2023
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228. A Novel Strategy for Accelerating Pumpable Ice Slurry Production with Ozone Micro-Nano Bubbles and Extending the Shelf Life of Larimichthys polyactis .
- Author
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Zhang R, Cheng Z, Liang Y, Hu X, Shen T, Li Y, Han Z, Zhang X, and Zou X
- Abstract
In this study, a novel strategy for accelerating the production of pumpable ice slurry (PIS) by using ozone micro-nano bubbles (O
3 -MNBs) was proposed. The effect of PIS containing sodium alginate (SA) and O3 -MNBs on the preservation of small yellow croaker ( Larimichthys polyactis ) was investigated. The results indicate that using SA solution containing O3 -MNBs instead of only SA solution resulted in quicker production of PIS by promoting ice nucleation and eliminating supercooling. The distribution and positive effect of O3 -MNBs as a nucleation agent on freezing characteristics were discussed. Microbial concentrations, pH, total volatile basic nitrogen, and thiobarbituric acid reactive substance content were also examined. Storage in novel PIS (containing O3 -MNBs) had higher performance than storage in flake ice or conventional PIS due to the strong bacteriostatic ability of O3 . Therefore, O3 -MNBs injection can be used as a novel method for PIS production and the preservation of fresh marine products.- Published
- 2023
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229. Effects of Pulsed Pressure Curing on Beef Quality.
- Author
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Li C, Shi J, Zhai X, Yang Z, Huang X, Li Z, Li Y, and Zou X
- Abstract
The study was proposed to investigate the effects of pulsed pressure curing on the beef absorption of the curing solution, cooking loss, moisture content, centrifugal loss, salt content, sensory attributes, texture, microstructures and volatile compounds. Curing methods include the following four treatments: (1) control group 1-static curing (SC); (2) control group 2-vacuum curing (VC); (3) control group 3-pressurized curing (PC); and (4) treatment group-pulsed pressure curing (PPC). The acquired results revealed that pulsed pressure curing significantly boosts the curing efficiency and moisture content, decreases cooking loss in beef, brightens meat color, and enhances texture compared to static curing, vacuum curing, and pressurized curing. Additionally, centrifugal losses were not impaired, and sensory findings revealed that PPC significantly improved the saltiness of beef. TPA results showed that the springiness and cohesiveness of PPC were greatly increased, and hardness and chewiness were significantly reduced. Moreover, PPC significantly reduced the content of 1-octen-3-ol and 1-hexanol. Scanning electron microscopy (SEM) images documented that pulsed pressure curing can effectively increase the tenderness of beef. This study demonstrates that processed meat product efficiency and sensory attributes should be taken into account when selecting a curing technique, and the PPC technique has an advantage in both areas.
- Published
- 2023
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230. Characterization and expression profiling of G protein-coupled receptors (GPCRs) in Spodoptera litura (Lepidoptera: Noctuidae).
- Author
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Li Y, Gao H, Zhang H, Yu R, Feng F, Tang J, and Li B
- Subjects
- Animals, Spodoptera metabolism, Insecta, Receptors, G-Protein-Coupled genetics, Reproduction, Larva, Moths
- Abstract
Spodoptera litura is a highly destructive omnivorous pest, and they caused serious damage to various crops. G protein-coupled receptors (GPCRs) mediate dozens of physiological processes including reproduction, development, life span and behaviors, but the information of these receptors has been lacking in S. litura. Here, we methodically identified 122 GPCRs in S. litura and made an assay of their expression patterns in different tissues. Comparing the identified GPCRs with homologous genes of other insects, it is obvious that the subfamily A2 (biogenic amine receptors) and the subfamily A3 (neuropeptide and protein hormone receptors) of S. litura have expanded to a certain extent, which may be related to the omnivorous nature and drought environment resistance of S. litura. Besides, the large Methuselah (Mth)/Methuselah-like (Mthl) subfamily of S. litura may be involved in many physiological functions such as longevity and stress response. Apart from duplicate receptors, the loss of parathyroid hormone receptor (PTHR) and the bride of sevenless (Boss) receptor in the lepidopteran insects may imply a new pattern of wing formation and energy metabolism in lepidopteran insects. In addition, the high expression level of GPCRs in different tissues reflects the functional diversity of GPCRs regulating. Systemic identification and initial characterization of GPCRs in S. litura provide a basis for further studies to reveal the functions of these receptors in regulating physiology and behavior., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Inc. All rights reserved.)
- Published
- 2022
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231. Conjunctiva Resident γδ T Cells Expressed High Level of IL-17A and Promoted the Severity of Dry Eye.
- Author
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Li L, Li Y, Zhu X, Wu B, Tang Z, Wen H, Yuan J, Zheng Q, and Chen W
- Subjects
- Animals, Humans, Mice, Conjunctiva metabolism, Disease Models, Animal, Inflammation metabolism, Mice, Inbred C57BL, Receptors, Antigen, T-Cell, gamma-delta, Dry Eye Syndromes metabolism, Interleukin-17 metabolism
- Abstract
Purpose: Conjunctival inflammation promotes ocular surface disorders in dry eye disease (DED). Here we identified γδ T cells as the predominant source of IL-17A in the murine conjunctiva and assessed their contribution to the pathogenesis of DED., Methods: We enrolled 22 patients with DED, and analyzed the proportion of γδ T cells in the conjunctival epithelial samples by flow cytometry. Adult C57Bl/6 wild-type and TCRδ-/- mice were used to induce DED models to investigate the role of γδ T cells. The characteristics of immune cell infiltration and the expression of immune-related cytokines or markers in mouse conjunctiva were analyzed by flow cytometry, Western blot, and quantitative polymerase chain reaction., Results: The proportion of γδ T cells in the human DED conjunctiva is significantly higher in patients with severe corneal epithelial defects than in mild ones, which is consistently observed in the murine DED model. Further, a high level of IL-17A but not IFN-γ is detected in the conjunctiva of mice. The increased murine IL-17A-producing cells on the conjunctiva are identified as γδ T cells predominantly and Th17 cells to a lesser extent. Ablation of γδ T cells by antibody depletion or genetic deletion of TCRδ alleviates ocular surface damage in the murine DED model., Conclusions: Our studies evaluate human and experimental murine DED for evidence of γδ T-cell-mediated inflammation and highlight a potential therapeutic synergy by targeting IL-17 and γδ T cells in DED treatment.
- Published
- 2022
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232. Light-Induced Selective Hydrogenation over PdAg Nanocages in Hollow MOF Microenvironment.
- Author
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Li L, Li Y, Jiao L, Liu X, Ma Z, Zeng YJ, Zheng X, and Jiang HL
- Abstract
Selective hydrogenation with high efficiency under ambient conditions remains a long-standing challenge. Here, a yolk-shell nanostructured catalyst, PdAg@ZIF-8, featuring plasmonic PdAg nanocages encompassed by a metal-organic framework (MOF, namely, ZIF-8) shell, has been rationally fabricated. PdAg@ZIF-8 achieves selective (97.5%) hydrogenation of nitrostyrene to vinylaniline with complete conversion at ambient temperature under visible light irradiation. The photothermal effect of Ag, together with the substrate enrichment effect of the catalyst, improves the Pd activity. The near-field enhancement effect from plasmonic Ag and optimized Pd electronic state by Ag alloying promote selective adsorption of the -NO
2 group and therefore catalytic selectivity. Remarkably, the unique yolk-shell nanostructure not only facilitates access to PdAg cores and protects them from aggregation but also benefits substrate enrichment and preferential -NO2 adsorption under light irradiation, the latter two of which surpass the core-shell counterpart, giving rise to enhanced activity, selectivity, and recyclability.- Published
- 2022
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233. A Novel Gas Sensor for Detecting Pork Freshness Based on PANI/AgNWs/Silk.
- Author
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Li Y, Li Y, Shi J, Li Z, Wang X, Hu X, Gong Y, and Zou X
- Abstract
A novel, operational, reliable, flexible gas sensor based on silk fibroin fibers (SFFs) as a substrate was proposed for detecting the freshness of pork. Silk is one of the earliest animal fibers utilized by humans, and SFFs exposed many biological micromolecules on the surface. Thus, the gas sensor was fabricated through polyaniline (PANI) and silver nanowires (AgNWs) and deposited on SFFs by in-suit polymerization. With trimethylamine (TMA) as a model gas, the sensing properties of the PANI/AgNWs/silk composites were examined at room temperature, and the linear correlativity was very prominent between these sensing measures and the TMA measures in the range of 3.33 μg/L-1200 μg/L. When the pork sample is detected by the sensor, it can be classified into fresh or stale pork with the total volatile basic nitrogen (TVB-N) as an index. The result indicated that the gas sensor was effective and showed great potential for applications to detect the freshness of pork.
- Published
- 2022
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234. Easy-to-Use Visual Sensing System for Milk Freshness, Sensitized with Acidity-Responsive N-Doped Carbon Quantum Dots.
- Author
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Hu X, Zhang X, Li Y, Shi J, Huang X, Li Z, Zhang J, Li W, Xu Y, and Zou X
- Abstract
This study established a flexible and eye-readable sensing system for the easy-to-use, visual detection of milk freshness, using acidity-responsive N-doped carbon quantum dots (N-CQDs). N-CQDs, rich in amino groups and with characteristic acidity sensitivity, exhibited high relative quantum yields of 25.2% and an optimal emission wavelength of 567 nm. The N-CQDs fluorescence quenching upon the dissociated hydrogen ions (H
+ ) in milk and their reacting with the amino groups produced an excellent linear relation (R2 = 0.996) between the fluorescence intensity and the milk acidity, which indicated that the fluorescence of the N-CQDs was highly correlated with milk freshness. Furthermore, a fluorescence sensor was designed by depositing the N-CQDs on filter-papers and starch-gel films, to provide eye-readable signals under UV light. A fluorescence colorimetric card was developed, based on the decrease in fluorescence brightness as freshness deteriorated. With the advantages of high sensitivity and eye readability, the proposed sensor could detect spoiled milk in advance and without any preprocessing steps, offering a promising method of assessing food safety.- Published
- 2022
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235. Manno-oligosaccharides from cassia seed gum ameliorate inflammation and improve glucose metabolism in diabetic rats.
- Author
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Wu C, Liu J, Li Y, Wang N, Yan Q, and Jiang Z
- Subjects
- Animals, Blood Glucose metabolism, Glucose metabolism, Inflammation drug therapy, Insulin, Plant Gums chemistry, RNA, Messenger, Rats, Seeds chemistry, Streptozocin, Cassia chemistry, Diabetes Mellitus, Experimental drug therapy, Diabetes Mellitus, Experimental metabolism, Oligosaccharides pharmacology
- Abstract
Functional oligosaccharides show anti-diabetic effects through inflammation regulation with improved glucose metabolism. In this study, novel prebiotics of manno-oligosaccharides from cassia seed gum (CMOS) were incorporated into the diet of streptozotocin (STZ) plus high-fat and high-sugar diet (HFSD)-induced rats. After feeding for 8 weeks, CMOS (300-1200 mg per kg b.w. per d) significantly ameliorated the fasting blood glucose level (7.1-8.2 mmol L
-1 ) as compared with that of the model group (14.2 mmol L-1 ), where the area under the oral glucose tolerance test curve was decreased by 20.0%-24.5%. Meanwhile, CMOS prevented STZ plus HFSD-induced damage to islet tissue with a clear and integrated morphology and reduced the glucagon/insulin area ratio (by 97.9% for 300 mg per kg b.w. per d CMOS). CMOS also reduced metabolic endotoxemia and maintained intestinal integrity with recovered mRNA expression of Zo-1 and occludin to the normal comparable level. Upon 16S rDNA sequencing, it was found that CMOS regulated the microbiota composition in the cecum with an increased relative abundance of Bifidobacteria , while that of Shigella was decreased. The molecular mechanisms involved in the anti-diabetic effects of CMOS were further studied. CMOS reduced the mRNA expression of Tlr2 and Tlr4 in the intestines of STZ plus HFSD-induced rats. Meanwhile, Nlrp3 associated inflammasome activation in the intestine and liver with glucose metabolism disorder was inhibited by CMOS, resulting in reduced interleukin-1β secretion (by 38.8-46.4% for CMOS of 300-1200 mg per kg b.w. per d) and inflammation. Furthermore, CMOS regulated the AKT/IRS/AMPK signaling pathway and improved glucose metabolism in the liver. Findings obtained here implicated that CMOS could modulate metabolic-inflammation as a functional dietary supplement.- Published
- 2022
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- View/download PDF
236. Response of xenobiotic biodegradation and metabolic genes in Tribolium castaneum following eugenol exposure.
- Author
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Zhang Y, Gao S, Zhang P, Sun H, Lu R, Yu R, Li Y, Zhang K, and Li B
- Subjects
- Animals, Eugenol metabolism, Eugenol pharmacology, RNA, Sequence Analysis, RNA, Xenobiotics metabolism, Xenobiotics pharmacology, Tribolium genetics, Tribolium metabolism
- Abstract
Eugenol, a plant-derived component possessing small side effects, has an insecticidal activity to Tribolium castaneum; however, the underlying molecular mechanisms of eugenol acting on T. castaneum are currently unclear. Here, a nerve conduction carboxylesterase and a detoxifying glutathione S-transferase were significantly inhibited after eugenol exposure, resulting in the paralysis or death of beetles. Then, RNA-sequencing of eugenol-exposed and control samples identified 362 differentially expressed genes (DEGs), containing 206 up-regulated and 156 down-regulated genes. RNA-seq data were validated further by qRT-PCR. GO analysis revealed that DEGs were associated with 1308 GO terms of which the most enriched GO terms were catalytic activity, and integral component of membrane; KEGG pathway analysis showed that these DEGs were distributed in 151 different pathways, of which some pathways associated with metabolism of xenobiotics or drug were significantly enriched, which indicated that eugenol most likely disturbed the processes of metabolism, and detoxication. Moreover, several DEGs including Hexokinase type 2, Isocitrate dehydrogenase, and Cytochrome b-related protein, might participate in the respiratory metabolism of eugenol-exposed beetles. Some DEGs encoding CYP, UGT, GST, OBP, CSP, and ABC transporter were involved in the xenobiotic or drug metabolism pathway, which suggested that these genes of T. castaneum participated in the response to eugenol exposure. Additionally, TcOBPC11/ TcGSTs7, detected by qRT-PCR and RNA-interference against these genes, significantly increased the mortality of eugenol-treated T. castaneum, providing further evidence for the involvement of OBP/GST in eugenol metabolic detoxification in T. castaneum. These results aid eugenol insecticidal mechanisms and provide the basis of insect control., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
- Published
- 2022
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237. Tracer-specific reference tissues selection improves detection of 18 F-FDG, 18 F-florbetapir, and 18 F-flortaucipir PET SUVR changes in Alzheimer's disease.
- Author
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Li Y, Ng YL, Paranjpe MD, Ge Q, Gu F, Li P, Yan S, Lu J, Wang X, and Zhou Y
- Subjects
- Amyloid metabolism, Aniline Compounds, Brain diagnostic imaging, Brain metabolism, Carbolines, Cross-Sectional Studies, Ethylene Glycols, Fluorodeoxyglucose F18 metabolism, Glucose metabolism, Humans, Positron-Emission Tomography methods, Alzheimer Disease diagnostic imaging, Alzheimer Disease metabolism, Cognitive Dysfunction diagnostic imaging, Cognitive Dysfunction metabolism
- Abstract
This study sought to identify a reference tissue-based quantification approach for improving the statistical power in detecting changes in brain glucose metabolism, amyloid, and tau deposition in Alzheimer's disease studies. A total of 794, 906, and 903 scans were included for
18 F-FDG,18 F-florbetapir, and18 F-flortaucipir, respectively. Positron emission tomography (PET) and T1-weighted images of participants were collected from the Alzheimer's disease Neuroimaging Initiative database, followed by partial volume correction. The standardized uptake value ratios (SUVRs) calculated from the cerebellum gray matter, centrum semiovale, and pons were evaluated at both region of interest (ROI) and voxelwise levels. The statistical power of reference tissues in detecting longitudinal SUVR changes was assessed via paired t-test. In cross-sectional analysis, the impact of reference tissue-based SUVR differences between cognitively normal and cognitively impaired groups was evaluated by effect sizes Cohen's d and two sample t-test adjusted by age, sex, and education levels. The average ROI t values of pons were 86.62 and 38.40% higher than that of centrum semiovale and cerebellum gray matter in detecting glucose metabolism decreases, while the centrum semiovale reference tissue-based SUVR provided higher t values for the detection of amyloid and tau deposition increases. The three reference tissues generated comparable d images for18 F-FDG,18 F-florbetapir, and18 F-flortaucipir and comparable t maps for18 F-florbetapir and18 F-flortaucipir, but pons-based t map showed superior performance in18 F-FDG. In conclusion, the tracer-specific reference tissue improved the detection of18 F-FDG,18 F-florbetapir, and18 F-flortaucipir PET SUVR changes, which helps the early diagnosis, monitoring of disease progression, and therapeutic response in Alzheimer's disease., (© 2022 United Imaging Healthcare. Human Brain Mapping published by Wiley Periodicals LLC.)- Published
- 2022
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238. In-Situ Silica Xerogel Assisted Facile Synthesis of Fe-N-C Catalysts with Dense Fe-N x Active Sites for Efficient Oxygen Reduction.
- Author
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Liu M, Wang L, Zhang L, Zhao Y, Chen K, Li Y, Yang X, Zhao L, Sun S, and Zhang J
- Abstract
In the past decade, atomically dispersed Fe active sites (coordinated with nitrogen) on carbon materials (FeNC) have emerged rapidly as promising single-atom catalysts (SACs) for the oxygen reduction reaction (ORR) to substitute precious group metal (PGM) catalysts, owing to their earth abundance and low cost. Nonetheless, the production of highly active FeNC SACs is largely restricted by material cost, low product yield and difficulty of microstructure design. Herein, the authors demonstrate a facile in-situ xerogel (ISG) assisted synthetic strategy, using cheap materials, to construct FeNC SACs (ISG FeNC). The porous silica xerogel, formed in-situ with the FeNC precursors, encourages the emergence of enormous micropores/mesopores and homogeneous confinement/protection to the precursors during pyrolysis, benefiting to the formation of abundant accessible active sites (27.6 × 10
19 sites g-1 ). Correspondingly, the ISG FeNC exhibits excellent ORR activity with a half-wave potential (E1/2 = 0.91 V) in alkaline medium. The Zn-air battery assembled using the ISG FeNC SACs as the bifunctional catalyst of air cathode, demonstrates commendable performance with high peak power density of 249.1 mW cm-2 and superior long-term stability (660 cycles with 220 h). This work offers an economic and efficient way to fabricate PGM-free SACs for diverse applications., (© 2022 Wiley-VCH GmbH.)- Published
- 2022
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239. Identification and characterization of G protein-coupled receptors in Spodoptera frugiperda (Insecta: Lepidoptera).
- Author
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Li Y, Gao H, Yu R, Zhang Y, Feng F, Tang J, and Li B
- Subjects
- Animals, Biological Evolution, Receptors, G-Protein-Coupled genetics, Receptors, G-Protein-Coupled metabolism, Spodoptera genetics, Spodoptera metabolism, Insecta, Neuropeptides genetics
- Abstract
Spodoptera frugiperda (Insecta: Lepidoptera) is a destructive invasive pest feeding on various plants and causing serious damage to several economically-important crops. G protein-coupled receptors (GPCRs) are cellular receptors that coordinate diverse signaling processes, associated with many physiological processes and disease states. However, less information about GPCRs had been reported in S. frugiperda, limiting the recognition of signaling system and in-depth studies of this pest. Here, a total of 167 GPCRs were identified in S. frugiperda. Compared with other insects, the GPCRs of S. frugiperda were significantly expanded. A large of tandem duplication and segmental duplication events were observed, which may be the key factor to increase the size of GPCR family. In detail, these expansion events mainly concentrate on biogenic amine receptors, neuropeptide and protein hormone receptors, which may be involved in feeding, reproduction, life span, and tolerance of S. frugiperda. Additionally, 17 Mth/Mthl members were identified in S. frugiperda, which may be similar to the evolutionary pattern of 16 Mth/Mthl members in Drosophila. Moreover, the expression patterns across different developmental stages of all GPCR genes were also analyzed. Among these, most of the GPCR genes are poorly expressed in S. frugiperda and some highly expressed GPCR genes help S. frugiperda adapt to the environment better, such as Rh6 and AkhR. In this study, all GPCRs in S. frugiperda were identified for the first time, which provided a basis for further revealing the role of these receptors in the physiological and behavioral regulation of this pest., (Copyright © 2022 Elsevier Inc. All rights reserved.)
- Published
- 2022
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240. Tailoring of electronic and surface structures boosts exciton-triggering photocatalysis for singlet oxygen generation.
- Author
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Zhang D, Wang P, Wang J, Li Y, Xia Y, and Zhan S
- Abstract
Arising from reduced dielectric screening, excitonic effects should be taken into account in ultrathin two-dimensional photocatalysts, and a significant challenge is achieving nontrivial excitonic regulation. However, the effect of structural modification on the regulation of the excitonic aspect is at a comparatively early stage. Herein, we report unusual effects of surface substitutional doping with Pt on electronic and surface characteristics of atomically thin layers of Bi
3 O4 Br, thereby enhancing the propensity to generate1 O2 Electronically, the introduced Pt impurity states with a lower energy level can trap photoinduced singlet excitons, thus reducing the singlet-triplet energy gap by ∼48% and effectively facilitating the intersystem crossing process for efficient triplet excitons yield. Superficially, the chemisorption state of O2 causes the changes in the magnetic moment (i.e., spin state) of O2 through electron-mediated triplet energy transfer, resulting a spontaneous spin-flip process and highly specific1 O2 generation. These traits exemplify the opportunities that the surface engineering provides a unique strategy for excitonic regulation and will stimulate more research on exciton-triggering photocatalysis for solar energy conversion., Competing Interests: The authors declare no competing interest.- Published
- 2021
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241. Biochemical Properties of a Novel D-Mannose Isomerase from Pseudomonas syringae for D-Mannose Production.
- Author
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Hua X, Li Y, Jiang Z, Ma J, Liu H, and Yan Q
- Subjects
- Aldose-Ketose Isomerases genetics, Hydrogen-Ion Concentration, Substrate Specificity, Temperature, Aldose-Ketose Isomerases metabolism, Mannose metabolism, Pseudomonas syringae metabolism, Pseudomonas syringae pathogenicity
- Abstract
D-Mannose isomerase can reversibly catalyze D-fructose to D-mannose which has various beneficial effects. A novel D-mannose isomerase gene (PsMIaseA) from Pseudomonas syringae was cloned and expressed in Escherichia coli. The recombinant D-mannose isomerase (PsMIaseA) showed the highest amino acid sequence homogeneity of 50% with ManI from Thermobifda fusca. PsMIaseA was purified through Ni-NTA chromatography, and its specific activity was 818.6 U mg
-1 . The optimal pH and temperature of PsMIaseA were pH 7.5 and 45 °C, respectively. The enzyme was stable within a wide pH range from 5.0 to 10.0. It could efficiently convert D-fructose to D-mannose without any metal ions. When PsMIaseA was incubated with 600 g/L D-fructose for 6 h, the space-time yield of D-mannose reached 27.2 g L-1 h-1 with a maximum conversion ratio of 27%. Therefore, the D-mannose isomerase may be suitable for green production of D-mannose.- Published
- 2021
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242. Adhesion of two-dimensional titanium carbides (MXenes) and graphene to silicon.
- Author
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Li Y, Huang S, Wei C, Wu C, and Mochalin VN
- Abstract
Two-dimensional transition metal carbides (MXenes) have attracted a great interest of the research community as a relatively recently discovered large class of materials with unique electronic and optical properties. Understanding of adhesion between MXenes and various substrates is critically important for MXene device fabrication and performance. We report results of direct atomic force microscopy (AFM) measurements of adhesion of two MXenes (Ti
3 C2 Tx and Ti2 CTx ) with a SiO2 coated Si spherical tip. The Maugis-Dugdale theory was applied to convert the AFM measured adhesion force to adhesion energy, while taking into account surface roughness. The obtained adhesion energies were compared with those for mono-, bi-, and tri-layer graphene, as well as SiO2 substrates. The average adhesion energies for the MXenes are 0.90 ± 0.03 J m-2 and 0.40 ± 0.02 J m-2 for thicker Ti3 C2 Tx and thinner Ti2 CTx , respectively, which is of the same order of magnitude as that between graphene and silica tip.- Published
- 2019
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243. A ratiometric fluorescence sensor for ultra-sensitive detection of trypsin inhibitor in soybean flour using gold nanocluster@carbon nitride quantum dots.
- Author
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Hu X, Shi J, Shi Y, Li W, Arslan M, Zhang W, Huang X, Li Z, Xu Y, Li Y, and Zou X
- Subjects
- Animals, Cattle, Flour analysis, Limit of Detection, Serum Albumin, Bovine chemistry, Trypsin chemistry, Gold chemistry, Metal Nanoparticles chemistry, Nitriles chemistry, Quantum Dots chemistry, Glycine max chemistry, Spectrometry, Fluorescence methods, Trypsin Inhibitors analysis
- Abstract
Gold nanocluster@carbon nitride quantum dot nanocomposites protected by bovine serum albumin (BSA-AuNC@CNQDs) were designed as a ratiometric fluorescence nanosensor for ultra-sensitive detection of trypsin inhibitor (TI). CNQDs were prepared via thermal treatment of carbon nitride powder. BSA-CNQDs acted as templates to synthesize BSA-AuNC@CNQDs with dual-emission peaks at 450 and 650 nm. Trypsin can catalyze the hydrolysis of BSA and decompose BSA-AuNC@CNQDs resulting in fluorescence quenching. The fluorescence quenching at 650 nm was prevented by the addition of TI to inhibit the activity of trypsin. The nanosensor-trypsin system showed a satisfactory ability toward TI detection. The ratiometric responses (the ratio of intensity at 650 to 450 nm, I
650 /I450 ) had an excellent linearity (R2 = 0.981) with logarithmic values of TI concentrations in the broad range of 1-10,000 ng/mL. The limit of detection (LOD, 0.089 ng/mL) indicates ultra-sensitive detection of TI can be achieved. Additionally, TI in soybean flour was detected by the proposed ratiometric method with satisfactory recoveries (98.15-105.52%) and less than 6% of coefficient of variation. This study reveals that BSA-AuNC@CNQDs have potential applications in detection of TI in real samples.- Published
- 2019
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244. Electrodeposition of gold nanoparticles and reduced graphene oxide on an electrode for fast and sensitive determination of methylmercury in fish.
- Author
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Xu Y, Zhang W, Shi J, Zou X, Li Y, Haroon Elrasheid T, Huang X, Li Z, Zhai X, and Hu X
- Subjects
- Animals, Electrochemical Techniques, Electrodes, Electroplating, Gold, Graphite, Metal Nanoparticles, Oxides, Fishes, Methylmercury Compounds analysis
- Abstract
Fish consumption is the main source of methylmercury (CH
3 Hg+ ) exposure for humans. In this study, gold nanoparticles and reduced graphene oxide (AuNPs-RGO) modified electrode was fabricated for determination of CH3 Hg+ in fish. The AuNPs-RGO composite was synthesized by electroreduction method. The composite was characterized by scanning electron microscopy and energy dispersive spectra, X-ray diffraction and Raman spectroscopy. Electrochemical performance of the proposed sensor was studied by cyclic voltammetry, electrochemical impedance spectroscopy and differential pulse stripping voltammetry. The excellent conductivity and large surface of graphene contributed to an improvement in the voltammetric stripping signal. Under the optimized conditions, the methylmercury concentration in the range from 3 to 24μgL-1 had a good linear relation with the peak current. The detection limit of AuNPs-RGO modified electrode was 0.12μgL-1 . Finally, the developed electrode was applied to detect methylmercury in fish samples, and the obtained results were in good agreement with certified values., (Copyright © 2017 Elsevier Ltd. All rights reserved.)- Published
- 2017
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245. Analyses of integrated aircraft cabin contaminant monitoring network based on Kalman consensus filter.
- Author
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Wang R, Li Y, Sun H, and Chen Z
- Abstract
The modern civil aircrafts use air ventilation pressurized cabins subject to the limited space. In order to monitor multiple contaminants and overcome the hypersensitivity of the single sensor, the paper constructs an output correction integrated sensor configuration using sensors with different measurement theories after comparing to other two different configurations. This proposed configuration works as a node in the contaminant distributed wireless sensor monitoring network. The corresponding measurement error models of integrated sensors are also proposed by using the Kalman consensus filter to estimate states and conduct data fusion in order to regulate the single sensor measurement results. The paper develops the sufficient proof of the Kalman consensus filter stability when considering the system and the observation noises and compares the mean estimation and the mean consensus errors between Kalman consensus filter and local Kalman filter. The numerical example analyses show the effectiveness of the algorithm., (Copyright © 2017 ISA. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2017
- Full Text
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246. A new sensor for ammonia based on cyanidin-sensitized titanium dioxide film operating at room temperature.
- Author
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Huang XW, Zou XB, Shi JY, Zhao JW, Li Y, Hao L, and Zhang J
- Subjects
- Electrodes, Spectrophotometry, Ultraviolet methods, Ammonia analysis, Anthocyanins chemistry, Biosensing Techniques methods, Temperature, Titanium chemistry
- Abstract
Design and fabrication of an ammonia sensor operating at room temperature based on pigment-sensitized TiO2 films was described. TiO2 was prepared by sol-gel method and deposited on glass slides containing gold electrodes. Then, the film immersed in a 2.5×10(-4)M ethanol solution of cyanidin to absorb the pigment. The hybrid organic-inorganic formed film here can detect ammonia reversibly at room temperature. The relative change resistance of the films at a potential difference of 1.5V is determined when the films are exposed to atmospheres containing ammonia vapors with concentrations over the range 10-50 ppm. The relative change resistance, S, of the films increased almost linearly with increasing concentrations of ammonia (r=0.92). The response time to increasing concentrations of the ammonia is about 180-220 s, and the corresponding values for decreasing concentrations 240-270 s. At low humidity, ammonia could be ionized by the cyanidin on the TiO2 film and thereby decrease in the proton concentration at the surface. Consequently, more positively charged holes at the surface of the TiO2 have to be extracted to neutralize the adsorbed cyanidin and water film. The resistance response to ammonia of the sensors was nearly independent on temperature from 10 to 50°C. These results are not actually as good as those reported in the literature, but this preliminary work proposes simpler and cheaper processes to realize NH3 sensor for room temperature applications., (Copyright © 2013 Elsevier B.V. All rights reserved.)
- Published
- 2013
- Full Text
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247. In vivo noninvasive detection of chlorophyll distribution in cucumber (Cucumis sativus) leaves by indices based on hyperspectral imaging.
- Author
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Zou X, Shi J, Hao L, Zhao J, Mao H, Chen Z, Li Y, and Holmes M
- Subjects
- Pigments, Biological chemistry, Plant Leaves chemistry, Chlorophyll analysis, Cucumis sativus chemistry, Spectroscopy, Near-Infrared
- Abstract
The objective of this study was to investigate the spectral behavior of the relationship between reflectance and chlorophyll content and to develop a technique for non-destructive chlorophyll estimation and distribution in leaves using hyperspectral imaging. The hyperspectral imaging data cube of cucumber (Cucumis sativus) leaves in the range of 450-850 nm was investigated and preprocessed. Sixty optical signatures or indices as a function of the associated reflectance (R(λ)) at the special wavelength (λ) nm which proposed in the literatures were used to predict the total chlorophyll content in cucumber leaves. Finally, R(710)/R(760), (R(780)-R(710))/(R(780)-R(680)), (R(750)-R(705))/(R(750)+R(705)), (R(680)-R(430))/(R(680)+R(430)), R(860)/(R(550)×R(708)), (R(695-705))(-1)-(R(750-800))(-1), and REP-LEM (a index based on red edge position and estimated with a linear extrapolation method) were identified as optimum indices. Red-edge waveband (680-780 nm) appeared in all these optimum indices, indicating the importance of REP (red edge position) in chlorophyll estimation. When (R(695-705))(-1)-(R(750-800))(-1), the best index was applied to an independent validation set, chlorophyll content (r=0.8286) were reasonably well predicted, indicating model robustness. Depending on the sample, this technique enables to identify and characterize the relative content of various chlorophyll that distribution in the cucumber leaves. The map shows a relatively low level of chlorophyll at margins. Higher level can be noticed in the regions along the main veins and in some areas exhibiting dark green tissue. Our results indicate that hyperspectral imaging has considerable promise for predicting pigments in leaves and, the pigments can be detected in situ in living plant samples non-destructively., (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Published
- 2011
- Full Text
- View/download PDF
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