201. Phenotypes and genotypes of campylobacter strains isolated after cleaning and disinfection in poultry slaughterhouses
- Author
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Christophe Soumet, Pierre Maris, Pascal Sanders, Marie-Bénédicte Peyrat, Laboratoire d'études et de recherches sur les médicaments vétérinaires et les désinfectants, Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Unité de Toxicologie des Contaminants, Laboratoire de Fougères - ANSES, and Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)
- Subjects
MESH: Abattoirs ,Disinfectant ,Antibiotics ,Colony Count, Microbial ,Campylobacter coli ,medicine.disease_cause ,Polymerase Chain Reaction ,Poultry ,MESH: Dose-Response Relationship, Drug ,MESH: Genotype ,Anti-Infective Agents ,MESH: Poultry ,Genotype ,Campylobacter Infections ,Prevalence ,MESH: Hygiene ,MESH: Animals ,0303 health sciences ,MESH: Microbial Sensitivity Tests ,biology ,Campylobacter ,Hygiene ,General Medicine ,[SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences ,Adaptation, Physiological ,3. Good health ,phénotype ,Phenotype ,France ,Public Health ,génotype ,MESH: Equipment Contamination ,MESH: Public Health ,Abattoirs ,Polymorphism, Restriction Fragment Length ,medicine.drug_class ,MESH: Anti-Infective Agents ,MESH: Consumer Product Safety ,Food Contamination ,desinfection ,Microbial Sensitivity Tests ,MESH: Poultry Diseases ,MESH: Phenotype ,Microbiology ,Campylobacter jejuni ,MESH: Campylobacter jejuni ,03 medical and health sciences ,Antibiotic resistance ,MESH: Campylobacter Infections ,Species Specificity ,MESH: Drug Resistance, Bacterial ,Drug Resistance, Bacterial ,medicine ,MESH: Species Specificity ,Animals ,Humans ,Typing ,MESH: Food Microbiology ,MESH: Colony Count, Microbial ,MESH: Prevalence ,Poultry Diseases ,030304 developmental biology ,MESH: Humans ,[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health ,General Veterinary ,Dose-Response Relationship, Drug ,030306 microbiology ,MESH: Polymorphism, Restriction Fragment Length ,desinfectant ,MESH: Polymerase Chain Reaction ,MESH: Campylobacter coli ,MESH: Food Contamination ,biology.organism_classification ,élevage ,MESH: Adaptation, Physiological ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,volaille ,MESH: France ,Consumer Product Safety ,Food Microbiology ,Equipment Contamination ,MESH: Campylobacter ,Bacteria - Abstract
Campylobacter is responsible for human bacterial enteritis and poultry meat is recognised as a primary source of infection. In slaughterhouses, cleaning and disinfection procedures are performed daily, and it has been suggested that disinfectant molecules might select for antibiotic resistant strains if shared targets or combined resistance mechanisms were involved. The aim of the study was to investigate if cleaning and disinfection procedures in poultry slaughterhouses select for antibiotic resistance in Campylobacter jejuni and C. coli and to determine the genotypes of isolates collected after cleaning and disinfection. Nine sampling visits were made to four French slaughterhouses. Samples were collected from transport crates and equipment surfaces, before and after cleaning and disinfection. Minimal inhibitory concentrations of the recovered C. jejuni and C. coli isolates to six antibiotics and two disinfectants were measured. The C. jejuni isolates collected from equipment surfaces after cleaning and disinfection were subjected to PCR-RFLP typing. Twenty-five C. jejuni isolates and 1 C. coli were recovered from equipment surfaces after cleaning and disinfection during five visits to three different slaughterhouses. Those isolates did not show an increased resistance to the tested antibiotics compared to isolates collected before cleaning and disinfection. Only one or two genotypes were recovered after cleaning and disinfection during single visits to each slaughterhouse. This observation suggests that such genotypes may be particularly adapted to survive cleaning and disinfection stress. Understanding the survival mechanisms of Campylobacter should facilitate the implementation of better-targeted strategies and reduce the public health burden associated with Campylobacter infection.
- Published
- 2007