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1,083 results on '"Kuwahara, T."'

206. Phase I Dose-escalation Clinical Trial of HF10 Oncolytic Herpes Virus in 17 Japanese Patients with Advanced Cancer

Author

Kasuya, H., Yasuhiro Kodera, Nakao, A., Yamamura, K., Gewen, T., Zhiwen, W., Hotta, Y., Yamada, S., Fujii, T., Fukuda, S., Tsurumaru, N., Kuwahara, T., Kikumori, T., Koide, Y., Fujimoto, Y., Nakashima, T., Hirooka, Y., Shiku, H., Tanaka, M., Takesako, K., Kondo, T., Aleksic, B., Kawashima, H., Goto, H., and Nishiyama, Y.

Subjects
Male, Oncolytic Virotherapy, Time Factors, Herpesvirus 1, Human, Middle Aged, Virus Replication, Oncolytic Viruses, Treatment Outcome, Japan, Neoplasms, Mutation, Humans, Female, Neoplasm Staging
Abstract

Oncolytic virus therapy is a promising new therapeutic method, one of an eagerly anticipated class of biological therapies against cancer. There are many different classes of oncolytic virus. One of these, herpes oncolytic virus, is strongly oncolytic and has a large DNA genome as 150k bp. HF10 is a spontaneous mutant of herpes simplex virus -1 (HSV-1) that replicates within tumors and destroys cancers without damaging normal tissue and organs. Clinical trials of HF10 are underway in Japan and the United States. The first pilot study of HF10 was initiated in Japan in 2003. This study examined the safety and efficacy of HF10 in the treatment of breast cancer and head and neck cancers; the trial also included careful dose escalation studies. In 2005, a clinical trial using HF10 to treat pancreatic cancer was initiated. screened In this Japanese study, 17 patients received HF10 in their tumor sites. A clinical trial in the United States is also ongoing to evaluate safety, tolerability and evidence of antitumor activity in patients with refractory superficial solid tumors. Here, we report the evaluation of the 17 patients treated in Japan. Among the patients, 6 had recurrent breast cancer, 3 had recurrent head and neck cancer, and 8 had non-resectable pancreatic cancer. No severe adverse side effects have been observed, and some therapeutic potential has been reported based on pathological findings, tumor markers, and diagnostic radiography. Those results should encourage further clinical trials of HF10 around the world.

241. Vol. 42, No. 1, pp.16-21, 2008 Decrease in the skin transparency induced by protein carbonylation in the stratum corneum.

Author

Iwai, I., Kuwahara, T., and Hirao, T.

Subjects
*SKIN care, *AMINO acids, *ORGANIC acids, *COSMETICS, *IMAGE analysis
Abstract

It has been reported in recent years that the carbonyl modification of protein plays a part in various diseases. The existence of the protein carbonylation in the stratum corneum also came to be known in the last few years, but the effect on the properties of the stratum corneum including the skin appearance was not clarified. In this study, we examined the influence of protein carbonylation in the stratum corneum to provide helpful information for skin care products focusing on transparency of the stratum corneum. Firstly, we developed a method to assess the protein carbonylation level numerically by the image analysis of tape-stripped stratum corneum reacted with a fluorescent hydrazide. The level of stratum corneum carbonylation of the sun-exposed site (face) was higher than that of unexposed site (upper arm), and the surface part of the stratum corneum showed a higher level of carbonylation compared with the deeper layer. Stratum corneum carbonylation was induced by in vitro UV irradiation. In addition, it was shown that the skin transparency score was significantly low in the woman with high level of protein carbonylation in the stratum corneum of the cheek. An experimentally carbonylated stratum corneum sheet in vitro became opaque. Among the amino acids investigated, L-lysine was the most effective to prevent carbonylation of the stratum corneum ex vivo. Moreover, L-lysine inhibited the decrease in the skin transparency caused by experimental carbonylation of the stratum corneum in the human skin. These results suggest that preventing the carbonylation of the stratum corneum due to adverse effects from the environment by l-lysine could improve the skin transparency. Keywords: stratum corneum, protein carbonylation, appearance, skin transparency, l-lysine, amino acids, fluorescent hydrazide, tape-stripping [ABSTRACT FROM AUTHOR]

Published
2009
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