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Objective To explore the effect of platelet rich fibrin （PRF）in the promotion of wound healing. Methods From June 2015 to October 2019, 30 patients with wounds were selected. All patients were divided into PRF treatment group and conventional dressing change group. All patients were followed up for 6 to 12 months after discharge. The treatment time, pain degree, satisfaction, total hospitalization expenses and complications of patients were recorded. Results In the PRF treatment group, the average treatment time were（20.53±12.69）days, the average frequency of dressing change were (3.76±1.66), the average treatment expenses were（19035.29±3993.07）yuan and the scores of pain were（3.01±1.63）. In the conventional dressing change group, the average treatment time（32.85±11.47）days, the average frequency of dressing change were（15.29±4.32）and the average treatment expenses were（25946.3±9425.1）yuan. Compared with the conventional dressing change group, the application of PRF can promote wound healing, reduce the frequency of dressing change, decrease the expenses of treatment and alleviate the pain during the therapeutic process. Conclusion The application of PRF is superior to the conventional dressing change in preventing wounds infection, shortening the treatment time, alleviating the pain and decreasing the expenses. The PRF has better therapeutic effect in the treatment of complex wounds. [ABSTRACT FROM AUTHOR]

Platelet preparations are autologous blood concentrates with supraphysiologic concentrations of platelets with or without leukocytes used in various clinical applications including regenerative medicine, wound healing, and facial rejuvenation. Their use harnesses the important role of platelets and their secretory products in immunoregulation, wound healing, angiogenesis, and tissue remodeling. The use of platelet preparations has been long documented in orthopaedic surgery, maxillofacial surgery, and other fields, but over the years research has increased in their application to facial plastic surgical techniques. Here we review the various formulations of platelet rich concentrates, their applications to facial skin rejuvenation, wound healing, scar reduction, and tissue fill as documented in the literature since 2018. [ABSTRACT FROM AUTHOR]

Background: Marginal tissue recession leads to exposure of root surfaces of teeth resulting in root sensitivity, caries, or an unsightly appearance. Its management is carried out both by eliminating contributing factors as well as using surgical techniques. Platelet-rich fibrin (PRF) is a seond-generation platelet concentrate first described by Choukroun et al. in 2001. This case report is of a Millers Class II recession in 41 regions managed using PRF with a double pedicle flap.Method: A 25-year-old patient presented with gingival recession height of 5 mm, measured from the cemento-enamel junction (CEJ) to gingival margin. The recession width was 3 mm at the CEJ and PD of 1 mm with respect to 41 region. There was no keratinized tissue apical to recession. However, adequate keratinized tissue was clinically present on either side of the recession defect wrt 41 region. A partial thickness double pedicle flap was raised wrt 41 region. PRF was prepared by centrifuging whole blood at 2,700 RPM for 12 min. The membrane was folded to create bulk and secured over the recession defect.Results: The patient was reviewed at regular intervals of 01, 06, months and 01 year postoperatively. Significant coverage of denuded root was achieved with good color match and relief from sensitivity. Results were stable one year postoperatively.Conclusion: Cases that present with a deep and wide recession are challenging to address owing to the extensive loss of keratinized tissue. In such cases, double pedicle flap can be carried out to augment gingiva by utilizing keratinized tissue adjacent to the defect site. Platelet concentrates like PRF contain platelet-derived growth factors that exhibit chemotactic and mitogenic properties that promote and modulate cellular functions involved in tissue healing, regeneration, and cell proliferation. PRF is an autologous biomaterial which may be used in root coverage procedures. Long-term studies with a larger sample size are required to establish PRF as a predictable method of gingival recession coverage. [ABSTRACT FROM AUTHOR]

Background: Platelet-rich fibrin (PRF) has been widely utilized in modern medicine and dentistry owing to its ability to rapidly stimulate neoangiogenesis, leading to faster tissue regeneration. While improvements over traditional platelet rich plasma therapies (which use chemical additives such as bovine thrombin and calcium chloride) have been observed, most clinicians are unaware that many tubes utilized for the production of 'natural' and '100% autologous' PRF may in fact contain chemical additives without appropriate or transparent knowledge provided to the treating clinician. The aim of this overview article is therefore to provide a technical note on recent discoveries related to PRF tubes and describe recent trends related to research on the topic from the authors laboratories. Methods: Recommendations are provided to clinicians with the aim of further optimizing PRF clots/membranes by appropriate understanding of PRF tubes. The most common additives to PRF tubes reported in the literature are silica and/or silicone. A variety of studies have been performed on their topic described in this narrative review article. Results: Typically, PRF production is best achieved with plain, chemical-free glass tubes. Unfortunately, a variety of other centrifugation tubes commonly used for lab testing/diagnostics and not necessarily manufactured for human use have been utilized in clinical practice for the production of PRF with unpredictable clinical outcomes. Many clinicians have noted an increased variability in PRF clot sizes, a decreased rate of clot formation (PRF remains liquid even after an adequate protocol is followed), or even an increased rate in the clinical signs of inflammation following the use of PRF. Conclusion: This technical note addresses these issues in detail and provides scientific background of recent research articles on the topic. Furthermore, the need to adequately select appropriate centrifugation tubes for the production of PRF is highlighted with quantitative data provided from in vitro and animal investigations emphasizing the negative impact of the addition of silica/silicone on clot formation, cell behavior and in vivo inflammation. [ABSTRACT FROM AUTHOR]

Much scientific evidence indicates the importance of the use of platelet-rich fibrin in the management of the healing of oral soft and bony tissues. The effectiveness of this substance is due to the growth factors and effective properties it contains in improving the healing and healing phase and relieving pain after surgery. Since the surgical extraction of the impacted third molars is one of the most common operations within the oral and maxillofacial surgeon's clinic, pain, and postoperative alveolitis are the most annoying complications for the patient. Present study aimed to assess the effect of platelet-rich fibrin after topical application in the cavity of the socket of the impacted lower thir d molar surgical extractions in smokers. Material and methods: 15 smoker patients who needed to have the lower third molars extracted under local anesthesia (n = 30) randomized with an X, O method for one of the two incisions. The two parts of the study gr oup are one in which platelet rich fibrin is applied within the socket cavity just before suturing and another where nothing is applied. Postoperative pain was assessed using a visual analogue scale scale on days 1, 2, 3, 5, and 7, and by the patient's dose of analgesic. The rate of alveolitis was evaluated, and then the variables were studied by comparison between the study gr oup and the control group. Results: In the study group, the visual analogue scale values were lower with a statistically significant difference compared with them in the control group on the first, second and third days after surgery (P <0.05). Conclusion: This study indicated that the topical application of platelet-rich fibrin within the socket of the impacted lower third molar after its extraction in smokers is an effective method in relieving symptoms following surgical extractions and that it contributed positively to reducing the incidence of alveolitis. [ABSTRACT FROM AUTHOR]

Background: There are many forms of scars that can result from inflammatory acne. It has a negative impact on the patient's personal and professional relationships. Acne scars are caused by the aberrant creation or breakdown of collagen that happens during healing processes. Atrophic scarring is the consequence in the majority of instances (80-90%) of collagen degradation at the dermal level. Hypertrophic or keloid scars are rarer manifestations of an enhanced collagen synthesis. Platelet rich fibrin (PRF) was introduced into the acne scar care picture after platelet-derived growth factor (PDGF) was proven to stimulate wound healing, angiogenesis, and tissue remodelling. Minimally invasive platelet-rich plasma methods provide the advantage of a fast recovery time and low cost. Objective: To examine the potential role of platelet rich fibrin in the treatment of post-acne scars. Methods: The databases were searched for articles published in English in 3 data bases [PubMed - Google scholar-Science direct] and Boolean operators (and, or, not) had been used such as [Platelet Rich Fibrin and Post Acne Sars OR PRF] and in peer-reviewed articles between 2009 and 2021. Documents in a language apart from English were excluded as sources for interpretation was not found. Papers apart from main scientific studies were excluded: documents unavailable as total written text, conversation, conference abstract papers and dissertations. Conclusion: Cell migration and proliferation are more efficient when fibrin is rich in platelets. When compared to platelet rich plasma, the fibrin matrix favours a slow and steady release of growth factors over time. [ABSTRACT FROM AUTHOR]

Most patients with cystic fibrosis have the risk of pathogenic bacteria being exchanged between their sinuses and lungs. A method for topical application of antibiotics where the antibiotics persist for a long period of time is needed. Ten patients with cystic fibrosis and bacterial sinusitis were included. Autologous platelet rich fibrin was mixed with an antibiotic solution and sprayed onto the mucosa at the end of an endoscopic sinus surgery; Colistin, a Ciprofloxacin-Colistin combination or Tobramycin was used. The antibiotic concentration was measured in the sinonasal mucus four, seven and 13 days after surgery. Nine patients had Pseudomonas aeruginosa in their nose/sinuses at the time of surgery; in eight of these P. aeruginosa was not detected by culture at the final visit. In the majority of the ten included patients the antibiotics were continuously released for more than 7 days. No severe side effects were seen. Autologous platelet rich fibrin co-delivered with antibiotics is a feasible method for topical antibiotic treatment in supplementary to sinus surgery. We expect that this treatment is successful for eradication of sinonasal bacterial infections in immunosuppressed patients suffering from recalcitrant sinus infections. The efficacy should be evaluated in randomized controlled trials. [ABSTRACT FROM AUTHOR]

Purpose: The purpose of this study was to evaluate the efficacy of intra-operative co-administration of tranexamic acid (TA) and platelet rich fibrin (PRF) using a proprietary co-delivery system on the amount of blood loss, early functional outcomes and wound complications after primary total knee arthroplasty (TKA). The intervention was compared to the standard of care (combined intravenous & topical TA) in a prospective, randomized, blinded setting.Methods: 80 patients undergoing primary cemented TKA without tourniquet were prospectively randomized into control (combined intravenous and topical TA) and PRF (intra-venous TA and co-delivery of topical PRF and TA) groups after informed consent. Total blood loss, drainage blood loss, knee range of motion, VAS pain scores, length of stay and wound complications were analysed. Data collection was performed in a double blind manner on days 1, 3 and 21.Results: There was no statistically significant difference in drainage blood loss (550 ml vs. 525 ml, p = 0.643), calculated total blood loss on day 1 (401 ml vs. 407 ml, p = 0.722), day 3 (467 ml vs 471 ml, p = 0.471) and day 21 (265 ml vs. 219 ml, p = 0.082) between the PRF and control groups respectively. The PRF group had a small but statistically significant increase in median knee extension in the early post-operative period, however this difference evened out at 3 weeks. No significant difference could be demonstrated between the PRF and control groups in length of stay, VAS pain scores, narcotic usage, wound complications and knee flexion at all time points.Conclusions: The topical co-delivery of PRF and TA does not significantly decrease blood loss in primary TKA compared to the standard of care. Slightly better active knee extension in the first 3 postoperative days can be achieved, however this benefit is not clinically relevant.Level Of Evidence: I, Therapeutic study. [ABSTRACT FROM AUTHOR]

Objective: To determine the efficacy of usage of topical autologous platelet rich fibrin (PRF) in improving outcomes of myringoplasty regarding graft uptake and hearing improvement. Methods: This study was conducted in the ENT department of Holy Family Hospital, Rawalpindi, over a period of six months from August 2019 to January 2020. It was designed as a prospective single blinded randomized controlled trial. The study involved a total of 50 participants in whom myringoplasty was done through post auricular approach using underlay technique. In 25 patients topical drops of PRF were used. Outcomes were compared after three months with the control group (n=25), who underwent myringoplasty without PRF. Results: After three months follow-up, graft uptake was reported 78% and 52% in cases and controls, respectively (P=0.070). Mean hearing improvement was 18 dB and 6 dB in cases and controls, respectively (P=0.014). Postoperative infection occurred in 8% of the cases, and in 32% controls (P=0.037). Conclusion: Topical use of Platelet-Rich Fibrin during myringoplasty results in improved graft uptake. Hence, resulting in much improved hearing, significant reduction in infection rates and decrease in perforation sizes. [ABSTRACT FROM AUTHOR]

The objective of this case report is to assess the use of autologous bone graft and platelet-rich fibrin (FRP), as guided bone regeneration, after the extraction of a retained canine. A 42-year-old female patient, with a history of controlled hypertension, attended the SERODU dental clinic (Ecuador), due to the presence of prominence in the left nasolabial region. A Computerized Axial Tomography (CT) was performed, in which tooth 1.3 included in the maxilla was observed, in a horizontal position, the crown mesially and the root distally. It was decided to carry out the extraction of the retained canine and the placement of regenerative biomaterials based on FRP and an autologous bone graft, obtained from the right maxillary tuberosity. Clinical and radiographic controls were carried out after surgery. Six months later, adequate healing and images compatible with bone regeneration were observed in the intervened area. [ABSTRACT FROM AUTHOR]

The authors present the case of a solitary mandibular cyst in a 12-year-old patient. A typically-placed cyst was discovered accidentally during a visit to the Orthodontic Outpatient Clinic. A revision of the affected area was performed under general endotracheal anesthesia due to the patient's age. There was clotted blood in the bone cavity and a small amount of soft tissue with a connective tissue-like gross appearance on the periphery of the cavity. The bone defect was filled with autogenous material. Histopathological examination of the biopsy and the clinical presentation confirmed the presence of a solitary cyst. [ABSTRACT FROM AUTHOR]

The growth factors present in platelets are important to guide the regenerating cells to the area of healing. Platelet-rich-fibrin (PRF) is one such material that holds on to these growth factors enmeshed in the fibrin network resulting in their sustained release over a period of time that can accelerate the wound healing process. With this knowledge, research has been carried out for a past few years for the clinical application of PRF. Various platelet concentrates have been studied including the platelet-rich-plasma (PRP). However, the short duration of cytokine release and its poor mechanical properties have resulted in the search of a new material with adequate properties for clinical application and ease of preparation. PRF has found a place in the regenerative field owing to its advantages over PRP. This was a cross-sectional study conducted among dental students undergoing training in saveetha dental college and hospitals. Data were collected by means of a selfadministered questionnaire consisting of 10 closed ended questions. The survey was prepared in the form of google forms .100 people have responded to the survey. From the results 88% of the respondents were aware of the various regenerative procedures. 74% of the respondents were aware of the platelet rich fibrin. The majority of the participants (54%) said PRF is an autograft and 35% said it is an allograft. 62% of the participants were not aware that PRF can be used in regenerative procedures. 88% of the respondents showed interest to attend a course on PRF. It is concluded from the results that students have limited knowledge regarding platelet rich fibrin. This shows the need for educational intervention to enhance the knowledge through interactive learning aids, seminars, workshops, and including the medical and dental significance of PRF as a part of the curriculum. [ABSTRACT FROM AUTHOR]

The ultimate goal of tissue engineering is to reproduce functional alveolar∖Periodontal complex. This study aimed to asses micro- and nano-formulated crystalline calcium sulfate as possible treatment of intrabony defect utilizing an autologous Platelet rich fibrin scaffold. and Methods: An experimental prospective split mouth design using eight healthy mongrel dog's was conducted. After two months of extracting the first premolar, bilateral intrabony defects were created mesial to the mandibular second premolar. One side was grafted by nanocrystalline calcium sulfate, while, the opposite side was grafted with microcrystalline calcium sulfate graft. Dogs were randomly euthanized after one month (group I) and 3 months (group II). The primary outcomes were the histological changes of new bone formation in hematoxylin and eosin and Masson trichrome stains, while histomorphometric and radiographic analysis were the secondary outcome followed by statistical analysis (P value was set at.05). There was a significant increase in the percentage of bone formation in relation to defect height in nanocrystalline compared to microcrystalline form after one month and three months in both histological (p-values of <0.0001) and radiographic results (p-values of <0.0001). Nanocrystalline calcium sulfate presented a significant enhanced periodontal regeneration compared to the microcrystalline form. [ABSTRACT FROM AUTHOR]

Tissue regenerative procedures aim to enhance regeneration of altered tissue. Extensive research has been carried out in this area and all proposed procedures present limitations. In this context the area of platelet-rich fibrin (PRF) research has gained tremendous awareness in the latest years. PRF is a low-cost regenerative modality that facilitates soft tissue regeneration derived from 100% autologous sources. It forms a fibrin mesh that liberates growth factors in a slow and prolonged manner and also contains supra-physiological concentrations of leukocytes. Reports from the literature have suggested that these leukocyte-rich blood-preparations are capable of improving wound healing, diminishing post-operative pain, and additionally minimizing the risk of infection. In our article we present the first clinical case where PRF was used as a wound healing accelerator of gingival lesions in a chemical soft tissue burn after teeth whitening. [ABSTRACT FROM AUTHOR]

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The clinical impact of platelet-rich fibrin (PRF) and plasma rich in growth factors (PRGF®) respectively has been studied extensively in the field of regenerative dentistry during the last two decades. Literature supports evidence for additional benefits in regenerative periodontal therapy, alveolar ridge preservation, management of extraction sockets, implantology including guided bone regeneration as well as defect management in oral surgery. Regarding gingival wound healing and soft tissue regeneration, there is sufficient evidence for their positive effects which have been confirmed in several systematic reviews. The effects seem less clear in conjunction with osseous regenerative treatments, where the inter-study heterogenity in terms of different PRF-protocols, indications and application forms might hinder a systematic comparison. Nevertheless there is evidence that PRF might have beneficial effects on hard-tissue or its regeneration respectively.For being able to facilitate conclusions in systematic reviews, precise reporting of the used PRF-protocols is mandatory for future (clinical) research in the field of autologous platelet concentrates., (Copyright 2024 SWISS DENTAL JOURNAL SSO – Science and Clinical Topics. License: This work is licensed under a Creative Commons Attribution 4.0 International License.)

The use of autologous platelet concentrates (APC) such as platelet-rich fibrin (PRF) and/or plasma rich in growth factors (PRGF®) is considered an established treatment modality in re-generative dentistry. The possibility of delivering growth factors over aclinically relevant time of several days seems particularly interesting in the context of wound healing.The growing body of evidence in the field of APC requires a continuous and actual knowledge of the literature for being able to make evidence-based treatment recommendations with a realistic assessment of possible advantages of this technology.PR(G)F can be applied in solid or liquid form, pure or in combination with other biomaterials. Both appear to be reasonable, depending on the clinical indication and/or desired treatment outcomes. Because of the many different factors that can affect the PR(G)F products final characteristics, a basic understanding of these parameters is desirable for choosing the most suitable product and/or optimizing its clinical application. This review aims to provide an over-view of relevant theoretical, practical, legal and biologic aspects of APCs., (Copyright 2024 SWISS DENTAL JOURNAL SSO – Science and Clinical Topics. License: This work is licensed under a Creative Commons Attribution 4.0 International License.)

Introduction: Autologous platelet concentrate (APC) are pro-angiogenic and can promote wound healing and tissue repair, also in combination with other biomaterials. However, challenging defect situations remain demanding. 3D bioprinting of an APC based bioink encapsulated in a hydrogel could overcome this limitation with enhanced physio-mechanical interface, growth factor retention/secretion and defect-personalized shape to ultimately enhance regeneration., Methods: This study used extrusion-based bioprinting to create a novel bioink of alginate/cellulose hydrogel loaded with thrombocyte concentrate. Chemico-physical testing exhibited an amorphous structure characterized by high shape fidelity. Cytotoxicity assay and incubation of human osteogenic sarcoma cells (SaOs2) exposed excellent biocompatibility. enzyme-linked immunosorbent assay analysis confirmed pro-angiogenic growth factor release of the printed constructs, and co-incubation with HUVECS displayed proper cell viability and proliferation. Chorioallantoic membrane (CAM) assay explored the pro-angiogenic potential of the prints in vivo . Detailed proteome and secretome analysis revealed a substantial amount and homologous presence of pro-angiogenic proteins in the 3D construct., Results: This study demonstrated a 3D bioprinting approach to fabricate a novel bioink of alginate/cellulose hydrogel loaded with thrombocyte concentrate with high shape fidelity, biocompatibility, and substantial pro-angiogenic properties., Conclusion: This approach may be suitable for challenging physiological and anatomical defect situations when translated into clinical use., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Grandjean, Perumal, Manicam, Matthey, Wu, Thiem, Stein, Henrich, Kämmerer, Al-Nawas, Ritz and Blatt.)

The objective was to compare pain relief in alveolar osteitis achieved by the application of platelet-rich fibrin (PRF) and aspirin cones, and to assess the influence of both treatments on bacterial concentrations in post-extraction wounds. A split-mouth, single-center, non-randomized controlled trial was conducted in 30 patients. Pain relief was assessed using the visual analog scale at three time points (before, 24 h after, and 48 h after intervention). Bacterial concentrations were evaluated from cultures of swab samples collected at the three time points. A Wilcoxon matched-pairs signed-rank test was performed to detect differences in distributions of scalar variables between treatment with PRF and aspirin cones. Pain relief at 24 h and 48 h after treatment was significantly higher (p = 0.003) with PRF application than with aspirin cone placement. Additionally, PRF application caused a significantly higher reduction in aerobic bacteria concentration, an average increase, instead of a decrease, in lactic acid bacilli concentration in the first 24 h, and a significantly greater increase in streptococci concentration at 48 h follow-up. PRF provides better pain relief than aspirin cones in alveolar osteitis. Bacterial concentrations in the extraction wounds are consistent with the pain relief achieved after PRF or aspirin application.

This experiment was conducted to investigate the accelerator effect of coral and platelet rich fibrin (PRF) on bone healing in rabbits (n=12) with clinically, radiologically, and histologically. The rabbits were divided randomly into two groups. There were two bone defects (3.5 mm diameter) created bilaterally on the proximal part of the tibia of rabbits. In control group, the defects were left empty. The other defects (twelve defects in each group) were filled with coral, PRF and coral plus PRF. Postoperatively, on the 30th and 60th days, clinical, radiographic and histologic examinations were performed. On the radiological examinations, bone healing was better seen in the grafted groups than in the control group (P

Trombositler hemostaz ve yara iyileşmesinde çok önemli bir rol oynamakta ve ihtiva ettikleri büyüme faktörleri ve sitokinler sayesinde rejeneratif tedavinin potansiyel hücreleri arasında yer almaktadırlar. Bu durum, rejeneratif tedavide, trombosit ve trombosit konsantrasyonlarına olan ilgiyi arttırmıştır. Geçmişten günümüze kadar çeşitli trombosit konsantrasyonları elde edilmiş ve bu ürünler tek başına veya başka biomateryallerle kombine kullanıldığında, iyileşme sürecine olan olumlu katkıları görülmüştür. Trombosit konsantrasyonları, sert ve yumuşak doku ile ilgili cerrahi prosedürlerde kullanıldığında, içeriğinde bulunan yoğun sitokin ve büyüme faktörlerinin kontrollü salınımı sağlar. Bu durum doğal yara iyileşme sürecini hızlandırmaktadır. Ayrıca, otojen kaynaklı olduklarından herhangi bir immünolojik reaksiyon ve enfeksiyona yol açmamaları en önemli avantajlarıdır. Tam kan farklı devirlerde ve farklı sürelerde santrifüj edildiğinde içeriği birbirinden farklı olan trombosit kaynaklı ürünler elde edilmiştir. PRP, PRGF, PRF ve CGF gibi ürünlere bakıldığında yeni keşfedilen her ürünün bir öncekine göre daha gelişmiş özellikleri olduğu görülmektedir. Bu derlemenin amacı; PRP, PRGF, PRF ve CGF gibi trombosit konsantrasyonlarının tarihsel gelişimi ve özellikleri hakkında bilgi vermektir.

Pyogenic granuloma (PG) is an inflammatory hyperplastic lesion of the oral cavity. The most common etiological factors for PG are low-grade local irritation, traumatic injury, or hormonal factors. Clinically, it appears to be smooth or lobulated exophytic lesion. It appears to be erythematous papule and is either pedunculated or has a sessile base. The lesion has shown a recurrence rate of 15.8%. Various treatment modalities have been present in the literature. The present article emphasized the treatment modalities and an effort to treat the recurrence of PG with the use of platelet-rich fibrin (PRF) membrane. This case report presents a 28-year-old female patient who had reported with PG that recurred twice in 2-year period. The lesion appeared to be recurrent with sessile base and was diagnosed clinically and histopathologically as PG. The PG was excised with the conventional scalpel method exposing the underlying bone. The exposed surface was then covered with PRF membrane. At 12-month follow-up period, the patient did not show any further recurrence of the lesion.

Background: The management of the furcation areas in multirooted teeth is often challenging due to difficulty in access. Platelet-rich fibrin (PRF), a second-generation platelet concentrate, has shown to accelerate the healing of soft and hard tissues. This study was designed to evaluate the efficacy of autologous PRF as a membrane in treatment of Grade II furcation defects in molars as compared to collagen membrane along with demineralized freeze-dried bone allograft in both the groups. Materials and Methods: A split-mouth study was planned with 18 patients having 2 sites of Grade II furcation defects each. Random allocation of the defect site was done for the test and control group. Plaque index, probing depth (PD), relative vertical clinical attachment level (RVCAL), gingival marginal level, and radiographic bone levels were recorded at baseline, 3 months, and 6 months postoperatively. Results: Both the groups showed statistically significant outcomes in intragroup comparison from baseline to 3 and 6 months. However, there was no statistical difference between PRF membrane and collagen membrane groups on intergroup comparison. Conclusion: There was a significant reduction of PD, improvement in RVCAL, and defect fill with autogenous PRF as membrane. This indicates its role as a regenerative material in treating furcation defects, which can be used as alternative to other expensive membranes.

Introduction: Attempts to regenerate the periodontal osseous defect, which is lost as a result of periodontal disease, require the tapping of the innate healing potential of periodontium through appropriately designed therapeutic strategies. A multitude of grafted and non-grafted approaches have been used in the management of Intra-bony defects. However, they do not provide predictable periodontal regeneration. The aim of this study was to evaluate the combined effect of low-level laser therapy (LLLT) and platelet-rich fibrin (PRF), in site modulated intra-bony defects (decortication), which were accessed using a simplified papilla preservation flap (SPPF), on the clinical and radiographic outcomes of periodontal disease. Methods: A total of 30 patients with intra-bony defects were recruited for the study and randomly distributed in two groups (n=15). Test group sites were accessed with SPPF and the defects received intra-marrow Penetration (IMP) following debridement and were irradiated with a low-level laser followed by PRF grafting and suturing done. The control group defects were accessed with SPPF and grafted with PRF before being secured by sutures. The plaque and bleeding score, PPD, CAL, and the position of the gingival margin with radiographic defect depth were recorded and analyzed at baseline and six months post-intervention using the student's t test and Wilcoxon signed rank test. Results: The test group showed a clinically relevant increase in mean PPD reduction, CAL gain, and radiographic bone fill (3.6 ± 1.35 mm, 3.26 ± 1.16 mm and 2.44 ± 1.24 mm) compared to the control group (2.93 ±1.1 mm, 2.267 ± 1.33 mm and 1.26 ± 0.99 mm) six months post-intervention. However, intergroup comparison between the test and control groups did not show any statistically significant difference. Conclusion: These results highlights that test protocol had greater amelioration of the effects of periodontal disease and all the investigated clinical and radiographic parameters showed considerable improvement from baseline to 6 months within test and control group, but intergroup comparison between the test and control groups did not show any statistically significant difference, indicating statistical equivalence between the test and control protocol. [ABSTRACT FROM AUTHOR]

The question of whether platelet rich fibrin and ozone can enhance regeneration of periodontal defect was addressed. three-wall periodontal defects were surgically created in 30 rats involving mesial aspect of right mandibular first molar. Rats were randomly assigned into three groups: 1) Group I (Positive control group). 2) Group II (Ozone treated group) and 3) Group III (PRF treated group). Two weeks after surgery, five rats from each group were euthanized and the remaining was euthanized 4 weeks post surgery. The degree of periodontal regeneration was evaluated using light microscope and scanning electron microscope. Histomorphometric measurements and anti-PCNA immunohistochemical counting were statistically analyzed. group I showed intense inflammatory reaction with mild new bone formation. In group II, partial regeneration was seen with moderate new woven bone formation in 2 weeks period. After 4 weeks, almost complete restoration of periodontium was seen. In group III, after 2 weeks, moderate lamellar bone formation was observed. In 4 weeks period, the periodontal regeneration was almost completed. Histomorphometric analysis showed a significant difference between group I and group II. The difference between group I and group III was significant in 2 weeks and highly significant after 4 weeks. That between group II and group III was nonsignificant in 2 weeks and significant in 4 weeks. Anti-PCNA analysis was nonsignificant between groups. both Platelet rich fibrin and ozone can improve histological parameters associated with healing of experimental intrabony periodontal defects in rats with the former being superior. [ABSTRACT FROM AUTHOR]

The present study evaluates and compares the effectiveness of Simvastatin (SIM), Hydroxyapatite (HA), and platelet-rich fibrin (PRF) in bone regeneration of periapical defects. Thirty-nine patients were selected and randomized into three groups, Group 1: HA (n = 13), Group 2: PRF (n = 13), Group 3: SIM (n = 13). After completion of RCT and apicoectomy, the grafts were placed locally in the defect and sutured. At the end of twelve months, postoperative symptoms and radiographic analysis assessed the outcome of the treatment Intragroup analysis of CBCT- Periapical Index (PAI) scores at 6th and 12th month revealed a significant change in the SIM group (p = 0.018 and 0.001 respectively), compared to PRF (p = 0.026 and 0.001 respectively) and HA (p = 0.053 and 0.039 respectively). Intergroup analysis of change in the level of CBCT-PAI score was highly significant (p = 0.003). SIM caused a more considerable change in the level of CBCT-PAI score compared to other groups, thereby indicating a faster rate of bone regeneration. [ABSTRACT FROM AUTHOR]

The aim of the study was to evaluate the influence of autologous platelet-rich fibrin (PRF) on soft tissue healing and bone regeneration following tooth extraction clinically and radio-graphically. 30 Patients between the age group of 18–40 years requiring extraction of bilateral mandibular molars except third molars were selected to conduct a split-mouth study after ethical approval. Teeth extraction was done on both sides in the same appointment. Autologous PRF was placed into the socket on one side randomly, and the socket on the other side was taken as control side. Parameters evaluated were soft tissue healing and bone regeneration. Soft tissue healing was evaluated on post-extraction day-3, day-7 and day-14 using healing index by Landry et al. Bone regeneration was assessed immediately and 4 months post-extraction by observation of change in radiopacity through digital panoramic-radiograph. Data obtained was statistically analysed and comparison of outcome variables was done using Mann-Whitney U-test, p < 0.05 was considered statistically significant. Case group had better soft tissue healing when compared to the control group with the p-value of 0.025 at 3rd day 0.039 at 7th day and 0.00 at 14th day. The rise in radiopacity at the end of 16th week for PRF group was higher as compared to control group but did not differ significantly. PRF is significantly better in promoting soft tissue healing and also hastens bone formation in extraction socket. PRF may be recommended as a valuable material for encouraging soft tissue healing and bone regeneration. [ABSTRACT FROM AUTHOR]

Being rich in growth factors platelet rich fibrin (PRF) has been used for decades for its healing properties in cosmetic surgeries but its use in transcanal myringoplasty is a novel approach that remains unexplored. In todays era of minimally invasive surgery not only does it provide as a great alternative to conventional myringoplasty but also outshines it. It reduces post operative pain, hospital stay and unlike the conventional technique that uses termporalis fascia as a graft material, PRF myringoplasty if required can be reperformed on the same patient multiple times with minimal risk. This procedure comes as a boon for patients with recurrent graft defects because after multiple revision surgeries, temporalis fascia can be deficient and other graft materials like cartilage/perichondrium or fascia lata etc. have to be harvested surgically for myringoplasty. In PRF myringoplasty there is no upper limit in the number of times the PRF membrane can be made and also it does not entail any surgical incision for graft harvest. A prospective study involving 41 patients was conducted at Netaji Subhash Chandra Bose Medical College, Jabalpur where in the tympanic membrane perforations were repaired using PRF membrane with a successful outcome in 85.4% of the patients included in this study. [ABSTRACT FROM AUTHOR]

Furcation involvement is the clinical finding that can lead to a diagnosis of advanced periodontitis and potentially to poor prognosis for the affected tooth or teeth. Grade II Furcation involvement, therefore, presents both diagnostic and therapeutic dilemmas. This review explains the vast aspects of Grade II furcation involvement in the form of aetiology, classification, diagnosis and different treatment modalities in detail. [ABSTRACT FROM AUTHOR]

Periodontitis causes bone loss leading intrabony defects and periodontal regeneration is the restoration of the periodontium to its original form and function. Various treatment modalities have been used in the past for periodontal regeneration and recently it has been found that melatonin, which is an endogenous hormone, has the potential to inhibit bone resorption and augment bone mass. It also down-regulates RANK-mediated osteoclast formation and activation, thereby increasing bone mass. Besides, it is a significant free radical scavenger and antioxidant. These distinctive properties of melatonin can be used for periodontal regeneration. Also, it has been established that PRF, which is a second-generation platelet concentrate, can be used for periodontal regeneration as it contains numerous growth factors like platelet‑derived growth factor and vascular endothelial growth factor. In the past, PRF has also been used in combination with bone grafts, but to our knowledge, it has not been used in combination with melatonin for intrabony defects. Therefore, in this case report, melatonin was used along with PRF in the treatment intrabony defect and positive clinical impact was seen. [ABSTRACT FROM AUTHOR]

To histologically evaluate the use of bovine derived deproteinized xenograft (DBBM), leukocyte and platelet rich fibrin (L-PRF) and the combination of both in Guided Bone Regeneration (GBR) performed in non-critical size defects in rabbit. A prospective experimental study was performed. Four bone defects in the tibiae of 12 rabbits were made and each of them was filled with DBBM, L-PRF, a combination of DBBM + L-PRF or was left to heal as control site. All defects were covered with a collagen membrane. Rabbits were randomly distributed in three groups and euthanatized at 3, 6 or 9 weeks. Samples were obtained and histologically analyzed to determine vital bone, connective tissue and remaining graft particles percentage. Analysis of variance, Kruskal Wallis and non-paired t-test where used to evaluate the significance of the results. At 3 weeks of healing, DBBM showed significantly more vital bone percentage than L-PRF (p = 0,05) and DBBM + L-PRF showed significantly less connective tissue than control (p < 0,05). All other groups showed no statistical difference between them. At 6 weeks of healing, DBBM showed significantly more vital bone percentage than L-PRF (p < 0,05) , DBBM + L-PRF (p < 0,05) and control (p < 0,05) and there wasn't any other significant difference regarding to connective tissue or remaining particle percentage between groups. At t 9 weeks healing period, there weren't any significant differences between groups. DBBM seems to enhance vital bone formation at early healing stages. The use of L-PRF alone or combined with DBBM, didn't show any histological improvement regarding to vital bone formation. The use of DBBM, alone or in conjunction with L-PRF showed a trend to reduce connective tissue percentage. The use of L-PRF combined with DBBM didn't affect the remaining particle percentage. [ABSTRACT FROM AUTHOR]

Palatoradicular grooves are usually found on the palatal or lateral roots of maxillary central and lateral incisors. Since clinical identification of these grooves are inaccessible and arduous in routine oral hygiene practices and are susceptible alcoves for microorganism habituation and plaque accumulation, it may result in acute to severe periodontitis and, if untreated, periapical pathosis also. This paper discusses about a female patient who reported pus discharge in left upper lateral incisor. Based on history, clinical examination and IOPA (intra-oral periapical radiograph), a deep pocket of about 10-14 mm was noticed in the left upper lateral incisor. A timely investigation was made and was treated surgically with advanced modified procedures which include odontoplasty and restoration of the defect with Platelet Rich Fibrin. After follow up of 6 months, the patient reported no signs of disease progression, had good oral hygiene and the tooth remained to be vital. [ABSTRACT FROM AUTHOR]

The ultimate goal of periodontal therapy is to regenerate the lost periodontal tissues caused by periodontitis. Platelet rich fibrin (PRF), a rich source of autologous growth factors and cytokines, is an upcoming therapeutic approach in the management of periodontal osseous defects. Combination of autologous PRF and the alloplastic bone substitutes holds a promising potential of enhanced bone regeneration. This case report includes the management of an intrabony osseous defect in relation to #21 with a combination of Platelet Rich Fibrin & bioactive glass (calcium phosphosilicatealloplast) with the clinical and radiographic evaluations using cone beam computed tomography (CBCT) for a 9 months follow up period. [ABSTRACT FROM AUTHOR]

Background: We aimed to investigate the effect of platelet-rich fibrin (PRF) on olfactory function and pain score in patients who underwent septoplasty. Methods: This prospective randomized observational study was performed between 2018 January and 2019 April with 148 patients who had septoplasty operation. Patients were divided two groups and 74 patients were placed in group 1 to which PRF was applied after the completion of septoplasty whereas 67 patients were put in group 2 which did not undergo PRF. Sniffin' Sticks test was applied to all patients at pre-op, post-op 1-week, 6-week, and 6-month. Pain scores of patients were measured with visual analogue scale at 1 and 3 week. Results: The distribution of patients according to pre-op olfactory function (normo-hypo-anosmia), there was no significant differences statistically (p > 0.05). When we compared the 1-week post-op results of Sniffin' Sticks test of patients, we found differences between the groups (p < 0.05). It was observed in the early postoperative period that according to the Sniffin' Sticks test scores, the results of the PRF group were better than those of the non-PRF group. At 6-week and 6-month, between the groups; there was no differences in terms of olfactory function. When we looked at the pain score of patients at 1 and 3 week after septoplasty; significant differences were obtained between groups. Conclusion: The application of PRF to the mucosal surface after the completion of septoplasty, has positive effect on olfactory function and pain especially in the early postoperative period. During the healing process, it was observed that prf maintained better odor functions. It is a minimally invasive technique with low risks and satisfactory clinical results. [ABSTRACT FROM AUTHOR]

Aim of the Study: The purpose of this study aimed to answer a clinical question whether the use of platelet rich fibrin after ridge splitting with simultaneous implant placement will enhance implant stability in comparison with xenograft or not. Materials and Method: sixteen patients were divided into 2 equal groups (Group I & Group II). Patients in both groups underwent surgical ridge splitting for narrow edentulous posterior mandibular ridge with simultaneous implant placement. In Group I, platelet rich fibrin used as a graft material around the implant in between buccal and lingual cortices. In Group II, Tutogen® xenograft used as a graft material around the implant in between buccal and lingual cortices. Each patient was assessed forimplant stability measured by OsstellTM by means of resonance frequency analysis (RFA)which is recodedas Implant Stability Quotient (ISQ)values first intraoperative then at intervals 3 months, 6months and 9 months postoperatively. Moreover the bone relative density has been evaluated by cone beam computed tomography (CBCT) immediate ostoperative and 6 months later. Results: A higher mean value of ISQ was recorded in Group II than in Group I with no statistically significant difference (p=0.246). In addition a higher mean value of bone density was recorded in Group I than in Group II with no statistically significant difference (p=0.601). Conclusion: Platelet rich fibrin could be used as grafting material from natural source, non-immunogenic, without xenopathy after ridge splitting in posterior mandibular region as a technique with time and cost saving with least morbidity. [ABSTRACT FROM AUTHOR]

Background: Recommendations for soft tissue management associated with customized bone regeneration should be developed. The aim of this study was to evaluate a new protocol for customized bone augmentation in a digital workflow. Methods: The investigators implemented a treatment of three-dimensional bone defects based on a customized titanium mesh (Yxoss CBR®, ReOSS, Filderstadt, Germany). Patients and augmentation sites were retrospectively analysed focussing on defect regions, demographic factors, healing difficulties and potential risk factors. An exposure rate was investigated concerning surgical splint application, A®- PRF and flap design. Results: In total, 98 implants could be placed. Yxoss CBR® was removed after mean time of 6.53 ± 2.7 months. Flap design was performed as full flap preparation (27.9%), full flap and periosteal incision (39.7%), periosteal incision (1.5%), poncho/split flap (27.9%) and rotation flap (2.9%). In 25% of the cases, exposures of the meshes were documented. Within this exposure rate, most of them were slight and only punctual (A = 16.2%), like one tooth width (B = 1.5%) and complete (C = 7.4%). A®- PRF provided significantly less exposures of the titanium meshes (76.5% no exposure vs. 23.5% yes, p = 0.029). Other parameters like tobacco abuse (p = 0.669), diabetes (p = 0.568) or surgical parameters (mesh size, defect region, flap design) did not influence the exposure rate. Surgical splints were not evaluated to reduce the exposure rate (p = 0.239). Gender (female) was significantly associated with less exposure rate (78,4% female vs. 21.6% male, p = 0.043). Conclusions: The results of this study suggest that the new digital protocol including patient-specific titanium meshes, resorbable membranes and bone grafting materials was proven to be a promising technique. To improve soft tissue healing, especially A®-PRF should be recommended. [ABSTRACT FROM AUTHOR]

The use of platelet-rich fibrin (PRF) is investigated in ulcer management because it provides a healing milieu rich in growth factors and cytokines. Although crucial, the relevance of secondary dressings is under-researched and no data support the use of any particular dressing in preference to another. We assessed the properties of different dressing categories, including alginates, hydrocolloids, foams, hydrofibers, films, meshes and gauzes, in terms of affinity for PRF, releasate management (retention/extrusion) and the kinetics of cytokine release as well as the influence of each combination product, [PRF + dressing], on dermal cell behaviour, aiming to provide useful information for choosing the most adequate dressing for each particular patient. Active dressings including alginates, hydrofibers, foams and hydrocolloids blend with PRF, creating a diverse combination of products with different performances. Alginate and hydrofiber showed the highest affinity but moderate retention of releasate, without interfering with cell functions. Instead, the foam sequestered the releasate and hindered the release of growth factors, thereby compromising cell activities. Film and mesh presented very poor releasate retention and performed similarly to PRF by itself. Affinity index and releasate management explained 79% of platelet-derived growth factor (PDGF-BB) concentration variability, p < 0.001. Cell proliferation depended on the ability of the combination product to retain/release supernatant, PDGF-BB concentration and cell adhesion R² = 0.91, p = 0.014. [ABSTRACT FROM AUTHOR]

Platelet concentrates have been widely used in regenerative medicine, including endodontics. The aim of this manuscript was to assess critically the efficacy of PRF in the treatment of endodontic periapical lesions in adult patients on the basis of the literature. The PICO approach was used to properly develop literature search strategies. The PubMed database was analyzed with the keywords: “((PRP) OR (PRF) OR (PRGF) OR (CGF)) AND (endodontic) AND ((treatment) OR (therapy))”. After screening of 155 results, 14 articles were included in this review. Different types of platelet concentrates are able to stimulate the processes of proliferation and differentiation of mesenchymal stem cells. Platelet rich fibrin (PRF) releases growth factors for at least 7 days at the application site. Growth factors and released cytokines stimulate the activity of osteoblasts. Moreover, the release of growth factors accelerates tissue regeneration by increasing the migration of fibroblasts. It was not possible to assess the efficacy of PRF supplementation in the treatment of endodontic periapical lesions in permanent, mature teeth with closed apexes, due to the lack of well-designed scientific research. Further studies are needed to analyze the effect of PRF on the healing processes in the periapical region.

Background and Objectives: The endodontic system and the periodontium are closely interrelated and the infection of both leads to the appearance of endodontic-periodontal lesions. Along with the endodontic and periodontal classic treatment, in most cases, there is a need for regenerative periodontal therapy for the repair of the damaged tissue. One material that stimulates bone healing is represented by platelet-rich fibrin (PRF). The aim of this study was to determine if the inclusion of PRF in the treatment protocol of endodontic-periodontal lesions is effective. Materials and Methods: This review was conducted according to the PRISMA guidelines. Four databases, MEDLINE (through PubMed), Scopus, Web of Science, and Google Scholar, were used in order to find all significant articles on the topic. Relevant keywords were used in different combinations. Results: The inclusion criteria were met by six studies, published between 2014 and 2020 and they were selected for the review. The use of PRF for the regenerative therapy of endodontic-periodontal lesions showed favorable outcomes in all of the studies included, with significant reductions in the probing depths. Conclusion: While platelet-rich fibrin may be beneficial, further research is needed.

Aim of the study To determine the effect of platelet-rich fibrin (PRF) on hard tissue healing following tooth extraction. Material and methods The study included 63 patients and a total of 183 extractions were performed. The study had a split-mouth design. Each patient had a study side where the sockets were filled with PRF and a control side, where the postextractional sockets underwent natural healing, by clot formation. After 3 months, for the evaluation of hard tissue quality, a bone density measurement was done using a Cone Bean Computer Tomogram (CBCT). Results Statistical analysis revealed a significant increase in bone density in the postextractional sockets where PRF was used as filling material. Conclusions It can be concluded that through the use of PRF as an augmentation material after tooth extraction the hard tissue healing process is significantly enhanced.

Osteomyelitis is inflammation of bone tissue which is seen in patient with malnutrition and such systemic diseases caused by decreased resistant to infection. Generally, chronic osteomyelitis is treated with antibiotics and surgical debridement to remove all the dead bone tissue. In this case report, the management of mandibular osteomyelitis which is developed after surgery and application with ozone and platelet-rich fibrin are presented.

Palatogingival groove also known as radicularlingual groove is a developmental anomaly involving the lingual surface of the maxillary incisors. They are inconspicuous, funnel-shaped, extend for varying distances on root surface and occur due to infolding of the hertwigs epithelial root sheath. This encourages adherence of microorganisms and plaque to levels significant for pathological changes resulting in endodontic and periodontal lesions. The variations in anatomy of the tooth as a cause of pulp necrosis in teeth of anterior maxillary segment should be considered by the clinician when other etiological factors are ruled out. Recognition of palatogingival groove is critical, especially because of its diagnostic complexity and the problems that may arise if it is not properly interpreted and treated. Regeneration is a new emerging approach in endodontics. Choukroun et al. were among the pioneers for using platelet-rich fibrin (PRF) to improve bone healing. PRF is rich in platelet cytokines and growth factors. Numerous techniques have been used to eliminate or seal the groove and regenerate endodontic and periodontal tissues. In this case report of two cases, a novel combination therapy involving ultrasonics, blend of PRF with bone graft, guided tissue regeneration membrane was used in the treatment of a palatogingival groove with an endoperio lesion to ensure arrest of disease progression and promote regeneration. The groove was cleaned and prepared ultrasonically and sealed with a bioactive dentin substitute.

In the modern day dental practice, synergy is fundamental. This synergistic effect must exist among various disciplines of dentistry for proper diagnosis, treatment planning, sequencing and execution of treatment in complex and challenging dental situations. Such collaborative effect between an orthodontist and a periodontist is essential as both works with same element, the tooth as crown-root unit with its supporting tissues. The orthodontic treatment is carried out through the medium of periodontium, so a healthy tooth supporting system is an essential prerequisite. Every potential candidate for orthodontic treatment should undergo a thorough periodontal examination. Any lousily diagnosed or conducted orthodontic treatment could be a facilitator of periodontal inflammatory or infectious process especially when the patient's oral hygiene is explicitly deficient. This case report demonstrates a challenging situation to a periodontist where patient had completed her orthodontic treatment but ended up with severe periodontal disease. The patient was thoroughly examined and a comprehensive treatment was planned and executed. Regenerative surgical procedures were done using platelet rich fibrin and hydroxyapitatite bone graft. Patient was followed up for 2 years. As sequelae of surgical procedures, patient had developed black triangles in the anterior region. The patient was unwilling for further perioplastic surgical procedures and further orthodontic treatment, so a gingival prosthesis using valplast was fabricated addressing her esthetic concerns.

The dental rehabilitation of a patient following single-tooth extraction in the esthetic zone is often clinically challenging. The treatment option for this is implant-supported single crown which has the advantage that adjacent teeth do not have to be prepared, as in a fixed prosthesis. This article presents a case of immediate implant placement in relation to 22 and a guided bone regeneration approach with platelet-rich fibrin (PRF) and bone graft. After immediate implant placement, to compensate for the gap (jumping distance) between the implant surface and bone walls of the socket, we used bone grafts and PRF clot as well as PRF membrane to achieve guided bone regeneration. PRF, which is the second generation platelet concentrate, offers the surgeon an access to growth factors with a simple and available technology. These growth factors, which are autologous, nontoxic, and nonimmunogenic, enhance and accelerate the normal bone regeneration pathways. The case presented showed successful outcome with a 1 year follow-up period.

Objective: In this study, we aimed to determine periimplantal tissue regeneration in the use of platelet-rich fibrin in periimplantal defects. Methods:Twelve male New Zealand white rabbits were used for this study. each of the right and left tibias of rabbits Using a trephine bur (diameter 7.0 mm), monocortical defects were prepared (7 mm width,4 mm depth).Subsequently, dental implants(AL-Technology dental implants sharkimplants) were installed into the left and right tibia (diameter 3.0 mm, length 8 mm). In the experimental groups, the peri-implant defect was filled respectively 1-autogenous grafts, 2-PRF 3-synthetic grafts (MIS 4BONE ). The control was left in an unfilled state. İmplant stability was evaluated with the resonance frequency analysis method (RFA)Results: The Ostell ISQ for the experimental groups respectively 1 -autogenous graft group 76.00±3.89,2-PRF group 75.00±3.84,3- sentetic graft group 67.00±5.72 while control group 56,33±5,12. The difference between the 2 groups (autogenous graft and prf groups) was statistically not significant while all other differences between groups are statistically significant.Conclusion: Within the limits of this study, it was found that periimplantal defects in the regeneration of Platelet-Rich Fibrin is effective as the autogenous graft

Background: Although regenerative medicine treatment is still in its infancy in Japan, legislation within the past decade has provided opportunities to explore new treatment methods using regenerative medicine. We conducted a 2-year prospective follow-up study to statistically analyze treatment outcomes using platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) as effective scaffolds in meniscal repair.Questions/Purposes.1. Does the use of PRF and PRP for meniscal repair surgery regenerate the meniscus?2. Does augmentation of meniscal repair with PRF effectively improve knee function outcomes?, Methods: Thirty-five patients underwent arthroscopic meniscal repair with PRP and PRF using new devices with all-inside techniques. Among these, 31 patients were evaluated for clinical outcomes postoperatively, and magnetic resonance imaging (MRI) findings were evaluated. The variables were coded appropriately by creating a new column based on the Kellgren-Lawrence classification over time. Log transformation of different time points (pre, post 6, 12, or 24 months) in relation to Lysholm, International Knee Documentation Committee (IKDC), Japanese Orthopedic Association (JOA), and Tegner scores were performed. Follow-up arthroscopy using a needle scope was performed in some cases 2 years later. The PRP and PRF used were characterized using the Minimum Information for Studies Evaluating Biologics in Orthopaedics classification., Results: Overall, 31 patients were evaluated (mean age = 39.1 ± 15.7 years; preoperative rehabilitation period = 9.5 ± 7.7 weeks). The PRP had mean platelet and white blood cell concentrations of approximately 5.5- and 3.6-fold, respectively. A significant association existed between sex and deformity deterioration but not in age between the two groups classified by deformity deterioration. Surgical intervention significantly and positively impacted knee function, although no significant changes were found in the activity of the participants over time. MRI findings showed no regeneration of the repaired meniscus; nevertheless, none of them worsened. Furthermore, a case where the traumatic cartilage defect was covered with fibrocartilage-like tissue as a secondary finding was observed., Conclusions: Meniscal repair surgery using PRP/PRF is an effective treatment option for improving knee function in patients with knee deformity., Level of Evidence: Level III, Non-randomized controlled cohort/follow-up study., Competing Interests: The author declares no conflicts of interest., (© 2024 The Author.)