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Lead is a metal that exists naturally in the Earth's crust and is a ubiquitous environmental contaminant. The alleviation of lead toxicity is important to keep human health under lead exposure. Biosynthesized selenium nanoparticle (SeNPs) and selenium-enriched Lactobacillus rhamnosus SHA113 (Se-LRS) were developed in this study, and their potentials in alleviating lead-induced injury to the liver and intestinal tract were evaluated in mice by oral administration for 4 weeks. As results, oral intake of lead acetate (150 mg/kg body weight per day) caused more than 50 times and 100 times lead accumulation in blood and the liver, respectively. Liver function was seriously damaged by the lead exposure, which is indicated as the significantly increased lipid accumulation in the liver, enhanced markers of liver function injury in serum, and occurrence of oxidative stress in liver tissues. Serious injury in intestinal tract was also found under lead exposure, as shown by the decrease of intestinal microbiota diversity and occurrence of oxidative stress. Except the lead content in blood and the liver were lowered by 52% and 58%, respectively, oral administration of Se-LRS protected all the other lead-induced injury markers to the normal level. By the comparison with the effects of normal L. rhamnosus SHA113 and the SeNPs isolated from Se-LRS, high protective effects of Se-LRS can be explained as the extremely high efficiency to promote lead excretion via feces by forming insoluble mixture. These findings illustrate the developed selenium-enriched L. rhamnosus can efficiently protect the liver and intestinal tract from injury by lead.

Aims Obesity and kidney diseases are common complex disorders with an increasing clinical and economic impact on healthcare around the globe. Our objective was to examine if modifiable anthropometric obesity indices show putatively causal association with kidney health and disease and highlight biological mechanisms of potential relevance to the association between obesity and the kidney. Methods and results We performed observational, one-sample, two-sample Mendelian randomization (MR) and multivariable MR studies in ∼300 000 participants of white-British ancestry from UK Biobank and participants of predominantly European ancestry from genome-wide association studies. The MR analyses revealed that increasing values of genetically predicted body mass index and waist circumference were causally associated with biochemical indices of renal function, kidney health index (a composite renal outcome derived from blood biochemistry, urine analysis, and International Classification of Disease-based kidney disease diagnoses), and both acute and chronic kidney diseases of different aetiologies including hypertensive renal disease and diabetic nephropathy. Approximately 13–16% and 21–26% of the potentially causal effect of obesity indices on kidney health were mediated by blood pressure and type 2 diabetes, respectively. A total of 61 pathways mapping primarily onto transcriptional/translational regulation, innate and adaptive immunity, and extracellular matrix and metabolism were associated with obesity measures in gene set enrichment analysis in up to 467 kidney transcriptomes. Conclusions Our data show that a putatively causal association of obesity with renal health is largely independent of blood pressure and type 2 diabetes and uncover the signatures of obesity on the transcriptome of human kidney.

Background DNA methylation (DNAm) is associated with gene regulation and estimated glomerular filtration rate (eGFR), a measure of kidney function. Decreased eGFR is more common among US Hispanics and African Americans. The causes for this are poorly understood. We aimed to identify trans-ethnic and ethnic-specific differentially methylated positions (DMPs) associated with eGFR using an agnostic, genome-wide approach. Methods The study included up to 5428 participants from multi-ethnic studies for discovery and 8109 participants for replication. We tested the associations between whole blood DNAm and eGFR using beta values from Illumina 450K or EPIC arrays. Ethnicity-stratified analyses were performed using linear mixed models adjusting for age, sex, smoking, and study-specific and technical variables. Summary results were meta-analyzed within and across ethnicities. Findings were assessed using integrative epigenomics methods and pathway analyses. Results We identified 93 DMPs associated with eGFR at an FDR of 0.05 and replicated 13 and 1 DMPs across independent samples in trans-ethnic and African American meta-analyses, respectively. The study also validated 6 previously published DMPs. Identified DMPs showed significant overlap enrichment with DNase I hypersensitive sites in kidney tissue, sites associated with the expression of proximal genes, and transcription factor motifs and pathways associated with kidney tissue and kidney development. Conclusions We uncovered trans-ethnic and ethnic-specific DMPs associated with eGFR, including DMPs enriched in regulatory elements in kidney tissue and pathways related to kidney development. These findings shed light on epigenetic mechanisms associated with kidney function, bridging the gap between population-specific eGFR-associated DNAm and tissue-specific regulatory context.

Passive satellite observations play an important role in monitoring global aerosol properties and helping quantify aerosol radiative forcing in the climate system. The quality of aerosol retrievals from the satellite platform relies on well-calibrated radiance measurements from multiple spectral bands, and the availability of appropriate particle optical models. Inaccurate scattering phase function assumptions can introduce large retrieval errors. The high-spatial resolution, dual-view observations from the advanced baseline imagers onboard the two most recent geostationary operational environmental satellites (GOES), East and West, provide a unique opportunity to better constrain the aerosol phase function. Using dual GOES reflectance measurements for a dust event in the Gulf of Mexico in 2019, we demonstrate how a first-guess phase function can be reconstructed by considering the variations in observed scattering angles throughout the day. Using the reconstructed phase function, aerosol optical depth retrievals from the two satellites are self-consistent and agree well with surface-based optical depth estimates. We evaluate our methodology and reconstructed phase function against independent retrievals made from low-Earth-orbit multi-angle observations for a different dust event in 2020. Our new aerosol optical depth retrievals have a root-mean-square-difference of 0.019–0.047. Furthermore, the retrievals between the two geostationary satellites for this case agree within about 0.059 ± 0.072, as compared to larger discrepancies between the operational GOES products at times, which do not employ the dual-view technique.

Background Lung adenocarcinoma is the leading cause of cancer death worldwide. Recently, ubiquitin C-terminal hydrolase L1 (UCHL1) has been demonstrated to be highly expressed in many tumors and plays the role of an oncogene. However, the functional mechanism of UCHL1 is unclear in lung adenocarcinoma progression. Methods We analyzed the differential expression of the UCHL1 gene in lung adenocarcinoma and normal lung tissues, and the correlation between the UCHL1 gene and prognosis was also analyzed by the bioinformatics database TCGA. Meanwhile, we detected and analyzed the expression of UCHL1 and Ki-67 protein in a tissue microarray (TMA) containing 150 patients with lung adenocarcinoma by immunohistochemistry (IHC) and clinicopathological characteristics by TCGA database. In vitro experiments, we knocked down the UCHL1 gene of A549 cells and detected the changes in cell migration, invasion, and apoptosis. At the same time, we analyzed the effect of UCHL1 on anti-tumor drug sensitivity of lung adenocarcinoma by a bioinformatics database. In terms of the detection rate of lung adenocarcinoma indicators, we analyzed the impact of UCHL1 combined with common clinical indicators on the detection rate of lung adenocarcinoma through a bioinformatics database. Results In this study, the analysis of UCHL1 protein expression in lung adenocarcinoma proved that obviously higher UCHL1 protein level was discovered in lung adenocarcinoma tissues. The expression of UCHL1 was closely related to poor clinical outcomes. Interestingly, a significantly positive correlation between the expression of UCHL1 and Ki-67-indicated UCHL1 was associated with tumor migration and invasion. Through executing loss of function tests, we affirmed that silencing of UCHL1 expression significantly inhibited migration and invasion of lung adenocarcinoma cells in vitro. Furthermore, lung adenocarcinoma cells with silenced UCHL1 showed a higher probability of apoptosis. In terms of the detection rate of lung adenocarcinoma indicators, we discovered UCHL1 could improve the detection rate of clinical lung adenocarcinoma and affect drug sensitivity. Conclusion In lung adenocarcinoma, UCHL1 promotes tumor migration, invasion, and metastasis by inhibiting apoptosis and has an important impact on the clinical drug treatment of lung adenocarcinoma. In addition, UCHL1 can improve the detection rate of clinical lung adenocarcinoma. Above all, UCHL1 may be a new marker for the diagnosis of lung adenocarcinoma and provide a new target for the treatment of clinical diseases.

This paper reports that the substrate-induced strain effect can be utilized to control spin Hall magnetoresistance (SMR) of YIG/Pt heterojunctions. The YIG films with in-plane compressive strain and tensile strain are respectively prepared on GGG and GSGG substrates by pulsed-laser deposition. The ratio of SMR of YIG/Pt heterojunctions with YIG under the in-plane tensile strain state is 1.6 time larger than that of YIG with the out-of-plane compressive strain state. The spin mixing conductance of YIG/Pt interface increases with the substrate-induced in-plane tensile strain on YIG. The increase of roughness and the reduction of ratio of Fe3+/Fe2+ for YIG surface is responsible for this modulation. This finding provides an interesting prospect for control of SMR by substrate-induced strain effect.

The d-band correlated rare-earth nickelate ( ReNiO3) is a typical quantum material that exhibits comparable reactivities to the noble metal oxide in oxygen evolution reactions (OER) for water splitting, apart from their well-known correlated electronic functionalities, such as metal to insulator transition. Nevertheless, the potential anisotropy in the catalyst reactivity of OER for ReNiO3 and its underneath mechanisms are yet under debate. Herein, we demonstrate the previously overlooked role associated with the surface atomic density of the Ni active-site that dominant in the anisotropic OER catalytic activities of ReNiO3. Despite its more localized electron configurations as indicated by the near edge x-ray absorption fine structure analysis and correlated transport, the OER catalytic activity was surprisingly observed to be higher for quasi-single crystalline NdNiO3 (001)/LaAlO3 (110), compared to that of NdNiO3(010)/LaAlO3 (001) and NdNiO3([Formula: see text]10)/LaAlO3 (111). This is attributed to the highest surface atomic density associated with the Ni active-site within NdNiO3 (001), compared to NdNiO3 (010) and NdNiO3 ([Formula: see text]10), and this kinetically reduces the overpotential of OER and the charge transfer resistance of NdNiO3 (001). The anisotropic OER activity sheds a light on the crystal orientation in the optimization of the ReNiO3 catalyst for water splitting.

Spintronic device based on spin–orbit torque (SOT) is a potential candidate for the next-generation memory and logic devices. Here, we report a SOT-based programmable multi-state logic-in-memory in a single unit. Multi-step magnetization switching behaviors can be achieved in the device with a stacking structure based on Pt/Co bilayers and an Al2O3 spacer layer by varying the thickness of Co and Pt layers. Moreover, five logic gates (NOR, OR, AND, NAND, and NOT) have also been realized by controlling the current and magnetic field. This multi-state logic-in-memory opens a simple and effective way for designing single-unit spintronic devices and extends the potential application of the SOT-based devices in brain-like computations.

Ferrimagnets with magnetic compensation temperature ( Tcomp) around room temperature are desirable due to their potential applications in low-energy consuming and high-frequency spintronic devices. In this study, the Tcomp of ferrimagnetic Mn2.21Ru0.86Ga (MRG) is tuned to near room temperature by strain. Moreover, we observed unconventional magnetoresistance behaviors for MRG-based Hall bar devices near Tcomp. First-principles calculations suggest two kinds of Mn moments, which lead to two anomalous Hall channels with opposite signs and consequently correspond to the peak structure and triple loops of the anomalous Hall effect loops. The unconventional temperature dependence of longitudinal resistivity is caused by the combined effects of two types of Mn moments and the anisotropic magnetoresistance of the MRG film. Interestingly, the spontaneous Hall angle of the MRG film is calculated to be ∼2.2%, which is one order of magnitude larger than those of other 3 d ferromagnets. Therefore, our study demonstrates MRG to be a ferrimagnet with the Tcomp near room temperature, which enables its potential applications in spintronic devices.

Multiple myeloma (MM) remains incurable with high rates of relapse. New therapeutic drugs are therefore urgently needed to improve the prognosis. JaponiconeA (JA), a natural product isolated fromCCK8 assays and flow cytometry were used to detect the proliferation, apoptosis, and cell cycle of MM cell lines when treated with JA.JA exhibited strong anti-tumor effects in MM. It sensitized myeloma cells to bortezomib and overcame NF-κB-induced drug resistance by inhibiting IKKβ, providing a new treatment strategy for MM patients.

The Hyper-Angular Rainbow Polarimeter (HARP) Cubesat started data collection in April 2020 from the ISS orbit and is the first Hyper-Angular imaging polarimeter in space. The HARP payload produces pushbroom images at four wavelengths (440, 550, 670 and 870nm) with up to 60 viewing angles at 670 nm and up to 20 along track angles for the other three wavelengths. HARP swath consists of 94 degs in the cross track direction, allowing for a very wide coverage around the globe, and +/-57 degs in the along track direction, providing wide scattering angle sampling for aerosol and cloud particle retrieval. The HARP satellite is still active on orbit and so far have produce a large collection of scenes providing an unprecedented demonstration of the hyperangular retrieval of cloud and aerosol properties from space. This presentation will discuss the performance of the HARP sensor in space, as well as its first results for aerosol and cloud measurements. HARP is preceded by its airborne version, the AirHARP instrument, which has flown in two NASA aircrafts to demonstrate the capabilities of the HARP payload. The HARP payload is also a precursor to the HARP-2 polarimeter that will fly on the NASA PACE mission to collect global data on aerosol and cloud particles.

Hydrological conditions drive the distribution of plant communities in wetlands to form vegetation zones where the material cycling varies with plant species. This mediation effect caused by the distribution of vegetation under hydrological conditions will affect the emission of N2O during the nitrogen migration in wetlands. In this study, five vegetation zones in the second largest wetland of China were investigated in situ during high and low water levels to elucidate the effect mediated by vegetation. With the increase in the rate of change of water levels, the zones of the mud flat, nymphoides, phalaris, carex, and reeds were distributed in sequence in the wetland, and the densities of carbon and nitrogen sequestrated by plants also increased. The carbon and nitrogen densities in each zone during low water level was significantly higher than that during high water level, while the organic carbon and the total nitrogen of sediments during high water level was higher. Sediments converted between source and sink for both carbon and nitrogen, during the annual fluctuation in water level. The flux in N2O emissions showed significant differences between the vegetation zones during each water level period. The emission flux decreased with the increasing C : N ratio in sediments, approximating the threshold at 0.23 μg m−2 h−1 when the C : N ratio > 25. The phylum abundance of Firmicutes, Proteobacteria, and Chloroflexi in sediments increased with flooding. The denitrifying nirS and nirK genes and anammox hzsB gene were significantly affected by water level fluctuation, with the maximal variations of these genes occurring in the mud flat and nymphoides zone. The results indicate that the distribution of plants under hydrological conditions modified the stoichiometric ratio of sediments, resulting in the variations of N2O emission fluxes and microbial communities in vegetation zones. Therefore, hydraulic regulation rather than direct planting would be an effective strategy to reduce greenhouse gas emissions in freshwater wetlands.

Although cyanobacteria blooms lead to an increase in methane (CH4) emissions in eutrophic lakes have been intensively studied, the methane production pathways and driving mechanisms of the associated CH4 emissions are still unclear. In this study, the hypereutrophic Lake Taihu, which has extreme cyanobacteria accumulation, was selected to test hypothesis of a potential methylotrophic CH4 production pathway. Field observation displayed that the CH4 emission flux from the area with cyanobacteria accumulation was 867.01 μg m−2·min−1, much higher than the flux of 3.44 μg m−2·min−1 in the non-cyanobacteria accumulation area. The corresponding abundance of methane-producing archaea (MPA) in the cyanobacteria-concentrated area was 77.33% higher than that in the non-concentrated area via RT-qPCR technologies. Synchronously, sediments from these areas were incubated in anaerobic bottles, and results exhibited the high CH4 emission potential of the cyanobacteria concentrated area versus the non-concentrated area (1199.26 vs. 205.76 μmol/L) and more active biological processes (CO2 emission, 2072.8 vs. −714.62 μmol/L). We also found evidence for the methylotrophic methane producing pathway, which contributed to the high CH4 emission flux from the cyanobacteria accumulation area. Firstly, cyanobacteria decomposition provided the prerequisite of abundant methyl thioether substances, including DMS, DMDS, and DMTS. Results showed that the content of methyl thioethers increased with the biomass of cyanobacteria, and the released DMS, DMDS, and DMTS was up to 96.35, 3.22 and 13.61 μg/L, respectively, in the highly concentrated 25000 g/cm3 cyanobacteria treatment. Then, cyanobacteria decomposition created anaerobic microenvironments (DO 0.06 mg/L and Eh −304.8Mv) for methylotrophic methane production. Lastly, the relative abundance of Methanosarcinales was increased from 7.67% at the initial stage to 36.02% at the final stage within a sediment treatment with 10 mmol/L N(CH3)3. Quantitatively, the proportion of the methylotrophic methane production pathway was as high as 32.58%. This finding is crucial for accurately evaluating the methane emission flux, and evaluating future management strategies of eutrophic lakes.

Phenylpropanoid (PPPN) compounds are widely used in agriculture, medical, food, and cosmetic industries because of their multiple bioactivities. Alternaria sp. MG1, an endophytic fungus isolated from grape, is a new natural source of PPPNs. However, the PPPN biosynthesis pathway in MG1 tends to be suppressed under normal growth conditions. Starvation has been reported to stimulate the PPPN pathway in plants, but this phenomenon has not been well studied in endophytic fungi. Here, metabolomics analysis was used to examine the profile of PPPN compounds, and quantitative reverse transcription-polymerase chain reaction was used to detect the expression of key genes in the PPPN biosynthesis pathway under starvation conditions. Starvation treatment significantly increased the accumulation of shikimate and PPPN compounds and upregulated the expression of key genes in their biosynthesis pathways. In addition to previously reported PPPNs, sinapate, 4-hydroxystyrene, piceatannol, and taxifolin were also detected under starvation treatment. These findings suggest that starvation treatment provides an effective way to optimize the production of PPPN compounds and may permit the investigation of compounds that are undetectable under normal conditions. Moreover, the diversity of its PPPNs makes strain MG1 a rich repository of valuable compounds and an extensive genetic resource for future studies.

Lofted mineral dust over data-sparse regions presents considerable challenges to satellite-based remote sensing methods and numerical weather prediction alike. The Southwest Asia domain is replete with such examples, with its diverse array of dust sources, dust mineralogy, and meteorologically-driven lofting mechanisms on multiple spatial and temporal scales. A microcosm of these challenges occurred over 3–4 August 2016 when two dust plumes, one lofted within an inland dry air mass and another embedded within a moist air mass, met over the Southern Arabian Peninsula. Whereas conventional infrared-based techniques readily detected the dry air mass dust plume, they experienced marked difficulties in detecting the moist air mass dust plume, which only became apparent when visible reflectance revealed it crossing over an adjacent dark water background. In combining information from numerical modelling, multi-satellite/multi-sensor observations of lofted dust and moisture profiles, and idealized radiative transfer simulations, we develop a better understanding of the environmental controls of this event, characterizing the sensitivity of infrared-based dust detection to column water vapor, dust vertical extent, and dust optical properties. Differences in assumptions of dust complex refractive index translate to variations in the sign and magnitude of the split-window brightness temperature difference commonly used for detecting mineral dust. A multi-sensor technique for mitigating the radiative masking effects of water vapor via modulation of the split-window dust-detection threshold, predicated on idealized simulations tied to these driving factors, is proposed and demonstrated. The new technique, indexed to independent-sensor description of the surface-to-500 mb atmospheric column moisture, reveals parts of the missing dust plume embedded in the moist air mass, with best performance over land surfaces.

Colorimetric, fluorescence, and paper-based method were developed to measure the Hg2+ level in water using iturin A, a lipopeptide produced by Bacillus subtilis. Firstly, iturin was used to synthesize highly stable and uniformly sized silver nanoparticles (AgNPs). Secondly, the iturin-AgNPs were found to be highly selective and sensitive to Hg2+. The absorbance of the reaction system showed a good linear correlation with the Hg2+ concentration from 0.5 to 5 mg/L at 450 nm in the UV-Vis spectroscopy detection with the limit of detection (LOD) of 0.5 mg/L. When the reaction system was detected by fluorescence measurement, a good linear relationship was found between the fluorescence intensity and Hg2+ concentration from 0.05 to 0.5 mg/ at 415 nm with the LOD of 0.05 mg/L. Lastly, a paper-based detection method was developed. The developed method was successfully used to detect Hg2+ in contaminated polluted waters and showed acceptable results in terms of sensitivity, selectivity and stability. The paper-based method could distinguish Hg2+ at levels higher than 0.05 mg/L, thereby meeting the guidelines of the effluent quality standard for industries (0.05 mg/L). In summary, this method can be used daily by various industries to monitor the Hg2+ level in effluent water.

Estuaries are considered hot spots for the production and emissions of nitrous oxide (N 2 O) and easily occur suspended particles (SPS), however, current understanding about the role of SPS in the N 2 O emissions from the oxic estuarine waters of lacustrine ecosystems is still limited. In this study, field investigations were performed in the estuaries of hypereutrophic Taihu Lake, and laboratory simulations were simultaneously conducted to ascertain the characteristics of N 2 O emissions with different SPS concentrations. The results showed that the N 2 O emission fluxes ranged from 9.75 to 118.38 μg m −2 h −1 , indicating a high spatial heterogeneity for the N 2 O emissions from the estuaries of Taihu Lake. Although the dissolved oxygen (DO) concentrations were up to 7.85 mg L −1 in the estuarine waters, from where the N 2 O emissions fluxes were approximately three times that of the lake regions. Multiple regression model selected the total nitrogen (TN), SPS, and DO concentrations as the crucial factors influencing the N 2 O emission fluxes. Particularly for SPS, the simulation results showed that the N 2 O concentrations increased gradually with the increase in the SPS concentrations of an oxic water column containing 4 mg L −1 of NO 3 − -N, indicating that a high SPS concentration can accelerate the N 2 O emissions. It was related to the change of denitrifying bacteria population in the SPS, as evidenced by its significantly positive correlation with N 2 O emissions ( p 2 O productions and emissions in eutrophic lakes, and its effect on nitrogen cycle should be considered in the future study.

Due to the inherent toxicity of cadmium selenide (CdSe)-based quantum dots (QDs), Cd-free alternatives are being widely investigated. Indium phosphide (InP) QDs have shown great potential as a replacement for CdSe QDs in display applications. However, the performance of InP-based quantum dot light-emitting diodes (QLEDs) is still far behind that of the CdSe-based devices. In this study, we wanted to show the effects of different approaches to improving the performance of InP-based QLED devices. We investigated the effect of magnesium (Mg) doping in ZnO nanoparticles, which is used as an n-type electron transport layer, in balancing the charge transfer in InP-based QLED devices. We found that an increasing Mg doping level can broaden ZnO band gap, shift its energy levels, but most importantly, increase its resistivity; as a result, the electron current density is significantly reduced and the device efficiency is improved. We also investigated the effect of high-photoluminescence quantum yield emitters and different QLED architectures on the device performance. Through optimizing QD structures and devices, red InP QLEDs with the current efficiencies as high as 11.6 cd/A were fabricated.

Multiple myeloma is a malignant plasma-cell disease, which is highly dependent on the hypoxic bone marrow microenvironment. However, the underlying mechanisms of hypoxia contributing to myeloma genesis are not fully understood. Here, we show that long non-coding RNA DARS-AS1 in myeloma is directly upregulated by hypoxia inducible factor (HIF)-1. Importantly, DARS-AS1 is required for the survival and tumorigenesis of myeloma cells both in vitro and in vivo. DARS-AS1 exerts its function by binding RNA-binding motif protein 39 (RBM39), which impedes the interaction between RBM39 and its E3 ubiquitin ligase RNF147, and prevents RBM39 from degradation. The overexpression of RBM39 observed in myeloma cells is associated with poor prognosis. Furthermore, knockdown of DARS-AS1 inhibits the mammalian target of rapamycin signaling pathway, an effect that is reversed by RBM39 overexpression. We reveal that a novel HIF-1/DARS-AS1/RBM39 pathway is implicated in the pathogenesis of myeloma. Targeting DARS-AS1/RBM39 may, therefore, represent a novel strategy to combat myeloma.

Pulsed laser deposition (PLD) is commonly used to grow thin film materials with complex stoichiometry and crystal structures, since it achieves a congruent transfer of the target cation elements to the substrate. Although the incongruent PLDs were previously observed via regulating the plasma/background interaction regimes, the incongruent PLDs were seldom investigated and applied practically for the thin film depositions. Herein, we demonstrate the incongruent pulsed laser deposition of Ca3Co4O9 thermoelectric thin films, via regulating the target compositions and the oxygen background pressures to preferential scattering the plasma cation compositions. To compare the deposition properties via incongruent and congruent thin film growth, we systematically investigate the crystal structures, surface morphologies, elemental distributions and thermoelectric transportation behaviors for Ca3Co4O9 thin films grown at various target compositions and background pressure ranges. A stoichiometric Ca3Co4O9 thin film composition is achievable via designing an incongruent PLD strategy, in which case the Ca/Co ratios in the targets are enlarged actively while the PLDs are performed at a pressure range of 100 Pa to further preferential scatter of the lighter Ca compared to Co. Nevertheless, the incongruently grown thin films exhibit lower thermoelectric performances, compared to the one at a similar composition grown via the congruent PLD strategy. This result may associate to the localized composition inhomogeneity in the Ca3Co4O9 grown incongruently, and their electronical resistivities are largely elevated by the resultant impurities that are hardly detectable by X-ray diffractions. The present work reveals the complexity in deposition mechanism and kinetics when performing incongruent PLDs, in particular, for growing multiple elemental thin film materials.

Particulate organic matter (POM) plays an important role in biological pumping as a source of energy and nutrients in aquatic systems, as well as being the mechanism for algal bloom formation. However, research on its sources and composition, particularly the research on the contribution of algae in the bloom season, is still insufficient. In this study, the sources and composition of the POM in the surface water of Lake Taihu during the algal bloom season were quantitatively analysed. Dual stable carbon (δ13C) and nitrogen (δ15N) isotopes revealed that the algae were not the only sources of POM during algal blooms, and their contributions were inconsistent in the different lake regions. On average, algae made up 57.2% of the POM in Lake Taihu. Due to wind farms and nutrient runoff, algal POM was mainly concentrated in Meiliang Bay, Gonghu Bay and Central Taihu. The proportions of algal POM in the western estuary area and East Taihu were the smallest with a minimum of 10.8%. The proportion of terrestrial POM in the surface water and vertical sections was 18.3% and 40.0%, respectively. Furthermore, the highest value was 76.8%, which accounted for a large proportion in the algal bloom season. The terrestrial POM that came from rivers was mainly concentrated in the western part of Lake Taihu, while the resuspension of sediments also increased the proportion of terrestrial sources in the surface water. Affected by the distribution of the vegetation in Lake Taihu, the proportion of macrophyte POM in East Taihu was higher than that of algal sources. Although the contribution of endogenous POM might further increase due to the dual effects of climate change and eutrophication, our isotopic evidence suggested that terrestrial POM cannot be ignored, even in the algal bloom season.

The antibiotic sulfadiazine (SDZ) is a challenging threat to the health of aquatic organisms, as it frequently occurs in aquatic ecosystems. Tolerance mechanisms and accumulation of SDZ in a floating macrophyte (Eichhornia crassipes) under hydroponic conditions were investigated in this study to provide more insight into the SDZ removal process. Results show that the presence of 1 mg L−1 SDZ decreased the quickest and ranged from 669.45 to 165.34 μg L−1 from days 5 to 25. Exposing E. crassipes to SDZ ( aerial parts under all treatments. These findings suggest that E. crassipes has the ability to phytoremediation SDZ contaminated water.

BACKGROUND Transcription factor 7 (TCF7) plays an essential role in Wnt signaling by interacting with s-catenin. Emerging evidence demonstrates that overexpression of TCF7 promotes progression or correlates with poor progression in several types of cancers, but the functions of TCF7 in gastric cancer (GC) have not been revealed. MATERIAL AND METHODS A total of 168 patients with GC who underwent radical surgeries were collected and regarded as the test cohort. The expression of TCF7 in the 168 patients was detected with immunohistochemistry. Moreover, the mRNA levels of TCF7 in 11 pairs of GC and adjacent tissues were detected with quantitative real-time PCR (qRT-PCR). The correlations between TCF7 and the clinicopathological factors were evaluated with the chi-square test, and the prognostic value of TCF7 in GC was investigated with univariate analysis and multivariate analysis. RESULTS The mRNA levels of TCF7 in GC tissues were significantly higher than in corresponding tumor adjacent tissues. The patients of low TCF7 expression and high TCF7 expression accounted for 76.79% (129/168) and 23.21% (39/168), respectively. In our experiments, TCF7 was significantly associated with positive lymphatic invasion (P=0.022) and metastasis (P

We report a strain-mediated giant magnetoresistance (GMR) in spin valves (SPVs) grown on shape memory alloys (SMAs). The SPVs with a stacking structure of Al2O3/Co90Fe10/Cu/Co90Fe10/IrMn/Pt were de...

In order to reduce energy consumption and further improve the performance of viscose yarns, this study introduced a collaborative control method to improve spun yarn performance by contacting the spinning strand with both a softening device and a pressure plate. In this study, we analyze the improving mechanism of spun yarn performance using the softening device and pressure plate. The results show that thermal insulation layer formed between the softening device and pressure plate could heat the yarns in all directions to further re-wrap out-exposed hairiness into the main body of yarns and save energy consumption. Four groups of 19.7 tex viscose yarns were spun with different collaborative apparatus (with and without the softening device or pressure plate). Four groups of viscose yarns were tested in terms of hairiness, unevenness, and tensile property. Moreover, the experimental results show that collaborative apparatus with the softening device and pressure plate could significantly improve yarn performance, including CV value, hairiness, break elongation, and breaking strength to 11.3%, 18.94, 12.9%, and 311.0 cN, respectively.

Emerging evidence indicates that natural killer (NK) cells and NKT-like cells may affect allograft outcomes following solid organ transplantation. However, the roles of these cells in allograft acceptance and dysfunction are controversial. To assess the changes in NK cell and CD3+CD56+ NKT-like cell frequency and phenotype in renal allograft recipients and to explore their associations with acute T-cell-mediated renal allograft rejection (ACR), longitudinal changes in NK and NKT-like cell frequency and phenotype were characterized using flow cytometry and immunohistochemistry in the peripheral blood and kidney allograft tissues in 142 recipients undergoing kidney transplantation. The serum concentrations of NK cell-associated cytokines were also detected by cytokine multiplex immunoassay. In contrast to the healthy controls, recipients with stable graft function exhibited increased proportions of CD56brightCD16dim subsets and decreased proportions of NKT-like cells in their peripheral blood mononuclear cells (PBMCs). Patients with ACR demonstrated increased proportions of NK cells, which were associated with increased CD3−CD56bright subsets and decreased CD3−CD56dim subsets, an increase in the CD56bright/CD56dim ratio in PBMCs and increased CD56+ NK cell infiltration in the kidney allograft, compared with the stable controls. In addition, there was a decreased proportion of NKT-like cells in patients with ACR, and an increased ratio of CD56bright/NKT-like cells compared with the stable controls. These differences appeared to be consistent with the increase in the serum concentrations of C-C motif chemokine 19 and the decrease in the serum concentrations of interleukin-15. These data indicate that CD56bright NK cells may promote the development of ACR, and that NKT-like cells may have immunoregulatory function. The results also imply that the CD56bright/CD56dim ratio may affect the ACR signatures.

It is essential to understand the combined effects of elevated CO2 and temperature on phytoplankton-zooplankton link when attempting to predict climate change responses of freshwater ecosystems. Phytoplankton species differ in stoichiometric and fatty acids composition, and this may result in phytoplankton-mediated effect on zooplankton at elevated CO2 and temperature. Beyond the isolated analysis of CO2 or temperature effect, few studies have assessed zooplankton growth under the phytoplankton-mediated effects of elevated CO2 and temperature. In this study, three algal species (green alga, diatom, cyanobacteria) were fed on zooplankton Daphnia magna, under the conditions of CO2 concentrations of ambient (390 ppm) and elevated (1000 ppm) levels and temperatures at 20, 25 and 30 °C. Elevated CO2 increased the algal biomass, while it reduced the phosphorus (P) and ω3 polyunsaturated fatty acids (ω3 PUFAs) to carbon (C) ratios. Elevated temperature decreased the P/C ratios in all algal cultures and ω3 PUFAs/C ratios in the diatom and the cyanobacteria cultures. Phytoplankton-mediated effect of elevated CO2 reduced the growth of zooplankton fed on the green and the mixed three algae culture. The stimulation of zooplankton fed on the diatom and the cyanobacteria by elevated temperature can be offset by decreasing food P and ω3 PUFAs contents. The combined effects of elevated CO2 and temperature on the growth of daphnids were mainly mediated by ω3 PUFAs/C ratios in the phytoplankton. Rising temperature as a combined direct and indirectly phytoplankton-mediated effect on zooplankton may be able to ameliorate the negative effects of elevated CO2. The results indicated that the combined effects of increased CO2 and temperature increased the fatty acid content of the green alga but not the other algae. This study highlighted that climate change with simultaneously increasing temperature and CO2 may entangle the carbon transfer in freshwater planktonic communities.

Iron deficiency anemia (IDA) is a common nutritional disease suffered by 1 billion people. To develop a new drug which avoids the side effects of traditional oral iron supplementation for IDA treatment, we have designed Fe3O4@ Astragalus polysaccharide core-shell nanoparticles (Fe3O4@APS NPs) and demonstrated them to be an efficient therapeutic drug for IDA treatment in vivo. The Fe3O4@APS NPs have been successfully synthesized with good water solubility and stability, especially in imitated digestion. Cytotoxicity assessment in cells and pathological tests in mice justify their good biocompatibility and low toxicity. The IDA treatment in rats shows that they have efficient therapeutic effect, which is contributed to both the iron element supplement from Fe3O4 and the APS-stimulated hematopoietic cell generation. Moreover, the superparamagnetic Fe3O4@APS NPs are capable for use as a magnetic resonance imaging contrast agent. This study presents the possibility of nanocomposites involving purified natural products from Chinese herb medicine for biomedical applications.

We report the enhancement of spin-orbit torque (SOT) in Pt/Co/Pt multilayers with inserting Ru layers. From current induced magnetization switching and harmonic measurements, we have found that both the damping-like and field-like effective fields increased with inserting Ru layers. The variation is firstly ascribed to the enhancement of the effective spin accumulation at the Co/Ru interfaces. Meanwhile, the interfacial Rashba effect has also been modulated, which depends on the direction of the internal electric field gradient at the interfaces. Our study provides an alternative way to modulate SOT.

In heavy metal (HM)/ferromagnetic metal (FM)/oxide heterostructures with perpendicular magnetic anisotropy (PMA), the magnetization can be efficiently controlled through spin orbit torque (SOT), which has attracted great attention since its applications in spintronic devices. In this work, we have investigated the SOT with modifying capping layers X in Pt/Co/X trilayers. In these systems, both the bottom Pt/Co and top Co/X interfaces are utilized to generate SOT via bulk SHE effect and/or Rashba effect. By the selecting of different capping layer X, one can expect an enhancement or cancellation of SOT contributions via interface effect, which provides an interesting prospect for enhancing the efficiency of SOT through a proper combination of materials in the structural engineering. Our experimental study explored the choice of the capping materials which influenced the magnetic behavior and current-induced magnetization switching process.

Lakes are regarded as one of the important sources of atmospheric CH4. However, the role of cyanobacteria blooms (CBBs) play in the CH4 production in eutrophic lakes is not fully clear. In this study, the spatial distribution characteristics of CH4 concentrations in surface water and sediment columns were investigated in Zhushan Bay of Taihu lake, China. Results showed that CH4 concentrations in CBBs accumulated zones were much higher than that in the open lake areas, with the highest values of 3.79 μmol·L−1 and 2261.88 μmol·L−1 in surface water and sediment columns, respectively. CH4 concentrations were strongly influenced by various factors. In surface water, the occurrence of CBBs greatly contributed to CH4 productions, as evidenced by the well-predicting for CH4 concentrations using Chl-a and NH4+ concentrations. In the sediments, the Ignition Loss and C:N ratio values were two indicators of CH4 contents, suggesting that the methanogenesis processes were influenced by not only the quantities, but also the qualities of organic matter. The labile substrates produced during the CBBs decomposition processes promoted the CH4 production and migration from sediments to the water column, resulting in the coherence in CH4 concentrations between the sediments and the surface water. The high-resolution determinations of CH4 concentrations in surface water and sediments clarified that the CBBs were a neglected facilitator of CH4 productions, which should be considered in the future estimation of CH4 emissions in eutrophic lakes.

Sediment resuspension has been recognized as a crucial internal process in aquatic ecosystems. However, there is still a lack of reliable measuring methods due to the complex hydrodynamic conditions in large shallow eutrophic lakes. In this study, sequential sediment traps (SST) and instantaneous multiple point (IMP) methods were compared at 6 sites located in the littoral zone of Zhushan Bay in Lake Taihu. Results show that the average resuspension rates (RRs) estimated using the IMP method at sites 1 to 6 were 266.39, 272.79, 235.17, 254.95, 392.25, and 483.85 g·m−2d−1, respectively. While the RRs estimated using the SST method were 195.16, 236.99, 116.76, 156.23, 389.53, and 509.85 g·m−2d−1, respectively. In wind-disturbed areas, both methods were suitable for RR analysis in large and shallow eutrophic lakes and SST provides high-resolution temporal RR estimations. However, in the areas with cyanobacterial blooms and vegetation cover, the IMP method overestimated the RR. Therefore, SST was more suitable across different conditions in large and shallow eutrophic lakes, providing a simple, accurate, and high-resolution temporal estimation of RR, while furthering our understanding of lake evolution processes.

Quercetin has been widely found to exhibit anticancer activity with low toxicity and prevalence in foods. Quercetin has been reported to inhibit digestive system cancers including pancreatic cancer (PAAD) and colon cancer (COAD), but rectal cancer (READ) has not been reported. The reported mechanisms and targets are divergent. In this study, new targets and mechanisms were predicted for the influence of quercetin on PAAD, COAD, and READ using bioinformatics methods. The results showed that quercetin may target CD36 and reduce the death rate caused by PAAD by enhancing the cell adhesion, mediating the uptake of fatty acids (FAs), regulating thrombospondin-1, and stimulating the immune response. Quercetin may lower the death rate from READ by targeting SLCO1B1 and producing enhanced effects from use of this compound, inhibiting cell growth, and inducing apoptosis in tumor cells. ACADS, ALDH3B2, UGT2A3, AMH, CDKN2A, FOSL1, CD36, CFL2, CYP3A4, and MAF were identified as targets for quercetin to reduce the death rate caused by COAD. Glutathione metabolism was mainly involved in the effect of quercetin on COAD, including the enhancement of the oxidation of fatty acids, the metabolism of anticancer medications, and the stiffness of cells, and the reduction of chemical carcinogenesis, the level of anti-Müllerian hormone, the proliferation of cancer cells and transcriptional misregulation, and mediation of the activity of glutathione transferases. The combined analyses of three databases can be referred to and used to seek medications and targets that can be applied to other diseases.

This paper reports magnetically recoverable Ag/Bi2Fe4O9 nanoparticles (BFO NPs) using as a photoexcited catalyst. The Ag NPs are uniformly distributed on the surface of BFO NPs. Compared to BFO NPs, the decomposition rate of methyl orange for Ag/BFO NPs under the visible-light is greatly enhanced due to the high surface plasmon resonance and electron conductivities of Ag NPs. Moreover, Ag/BFO NPs have a higher saturation magnetization than BFO NPs, which makes them can be collected easily under magnetic fields. Therefore, Ag/BFO NPs are potential recoverable visible-light-driven photocatalyst for environmental pollutants degradation.

As a basic and fundamental problem in wireless sensor network (WSN), the network coverage greatly reflects the performance of information transmission in WSN. In order to achieve a good balance between target coverage and energy consumption, in this paper, we propose a novel wireless sensor network energy efficient coverage method based on genetic algorithm. Particularly, the goal of this work is cover a 2D sensing area via selecting a minimum number of sensors. Moreover, the deployed wireless sensors should be connected to let each sensor be connected a path to the base station. Afterwards, genetic algorithm is used to compute the minimum number of potential position to let all target be k-covered and all sensor nodes be m-connected, and each chromosome is set to be the number of potential positions. Finally, we provide a simulation to test the performance of the proposed method, and simulation results demonstrate that the proposed method can achieve high degree of target coverage without wasting extra energy.

Estuarine wetlands play important roles in the regional and global carbon cycle as well as greenhouse gas emissions; however, the driving factors and potential carbon emissions mechanisms are unclear. Here, the carbon emission fluxes were investigated in situ from different vegetated areas in the Chongming wetlands. The results showed that the highest methane (CH