200 results on '"Vadgama, Pankaj"'
Search Results
152. Problems of clinical data interpretation
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Vadgama, Pankaj, primary, Desai, Mohamed, additional, Koochaki, Zarah, additional, and Treloar, Paul, additional
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- 1991
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153. Matrix surface modification by plasma polymerization for enzyme immobilization
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Mutlu, Mehmet, primary, Mutlu, Selma, additional, Rosenberg, Mark F., additional, Kane, John, additional, Jones, Malcolm N., additional, and Vadgama, Pankaj, additional
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- 1991
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154. Measurement of effective HCl diffusion coefficients through aqueous and gel films by a pH jump technique
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Nicholas, Christopher V., primary, Desai, Mohamed A., additional, Vadgama, Pankaj M., additional, Lucas, Sam, additional, and McDonnell, Martin B., additional
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- 1991
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155. Oxygen Detection Using Different Types of Membranes Deposited on Needle Based Platforms
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Anastasova, Salzitsa, Spehar, Maria, and, Deleze, and Vadgama, Pankaj
- Abstract
The aim of this work was to develop needle based sensors for oxygen sensing using different types of membranes. For oxygen sensors it is important to achieve fast response time, good linearity, low biofouling and high sensitivity. The effect of different types of polymeric barrier membranes and their various combinations were assessed and compared. An optimized system was thereby selected: sensors covered with polyurethane membrane doped with surfactant showed good stability with a linear working range up to 750 mmHg pO2.
- Published
- 2012
156. Urea pH electrodes: characterisation and optimisation for plasma measurements.
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Vadgama, Pankaj
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- 1986
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157. Amperometric enzyme electrode system for extracorporeal lactate monitoring based on lactate dehydrogenase.
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Vadgama, Pankaj, Covington, Arthur K., and Alberti, Kurt G. M. M.
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- 1986
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158. Enzyme electrode for glucose based on the quinoprotein glucose dehydrogenase.
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Mullen, William H., Churchouse, Stephen J., and Vadgama, Pankaj M.
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- 1985
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159. Polypyrrole incorporating biomolecules
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Day Ateh, Davidson, Lillie, Geoffrey Charles, and Vadgama, Pankaj
- Abstract
Polypyrrole (PPy) is a conducting polymer with emerging applications in biomedical engineering. We report on the synthesis and properties of PPy incorporating biomolecules using AC impedance spectroscopy. Impedance behaviour was consistent with two basic charge transfer processes, polaronic and electronic, demonstrated by two-electrode impedance spectroscopy over the frequency range 5 Hz?13 MHz. These processes were sensitive to alterations in environmental conditions such as pH and ionic strength. The reported results are likely to be a consequence of the intimate molecular association between PPy chains and incorporated molecules in the matrix as evidenced by variations in impedance spectra related to polymer loading. PPy incorporating physically entrapped biomolecules together with impedance spectroscopy could be exploited in reagentless biosensor applications. Furthermore, this could be coupled to their emerging role in cell contact and guidance applications for the detection of cell adhesion, migration or metabolite monitoring.
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- 2008
160. Assessing the carcinogenic potential of low-dose exposures to chemical mixtures in the environment: the challenge ahead
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Wade, Mark, Gonzalez, Michael J., Brooks, Samira A., Gonzalez, Laetitia, Sabbisetti, Venkata, Weisz, Judith, Felsher, Dean W., Odero-Marah, Valerie, Sone, Hideko, Goldberg, Gary S., Ivana Scovassi, A., Hsu, Hsue Yin, Wolf, Gregory T., Kim Lyerly, H., Eltom, Sakina, Hamid, Roslida Abd, Prudhomme, Kalan R., Chiaradonna, Ferdinando, Calaf, Gloria M., Al-Temaimi, Rabeah, Miousse, Isabelle R., Krishnan, Harini, Kravchenko, Julia, Engström, Wilhelm, Ogunkua, Olugbemiga, Van Larebeke, Nik, Rojas, Emilio, Collins, Andrew R., Ryan, Elizabeth P., Bay, Sarah N., Martinez-Leal, Juan Fernando, Darroudi, Firouz, Vento, Renza, Nangia-Makker, Pratima, Charles, Amelia K., Lowe, Leroy, Van Schooten, Frederik J., Goodson, William H., Eriksson, Staffan, Woodrick, Jordan, Sun, Jun, Ghosh, Paramita M., Carlin, Danielle J., Ochieng, Josiah, Hamid, Hasiah Ab, Rojanasakul, Yon, Manjili, Masoud H., Vadgama, Pankaj, Lee, Tae Jin, Carnero, Amancio, Martin, Francis L., Castellino, Robert C., Carpenter, David O., Valverde, Mahara, Christopher, Joseph A., Kimryn Rathmell, W., Ryeom, Sandra, Cruickshanks, Nichola, Koch, Daniel C., Ponce-Cusi, Richard, Heneberg, Petr, Brown, Dustin G., Dormoy, Valérian, Gilbertson, Michael, Langie, Sabine A.S., Koturbash, Igor, Otsuki, Takemi, Naus, Christian C., Mehta, Rekha, Chen, Zhenbang, Ostrosky-Wegman, Patricia, Massfelder, Thierry, Zhou, Binhua P., McCawley, Lisa J., Harris, Shelley A., Casey, Stephanie C., Xia, Menghang, Romano, Maria Fiammetta, Whitfield, Jonathan R., Laird, Dale W., Li, Lin, Dong, Chenfang, Brooks Robey, R., Pavanello, Sofia, Ratovitski, Edward, Roman, Jesse, Lleonart, Matilde, Vondráček, Jan, Narayanan, Kannan Badri, Marignani, Paola A., Brunborg, Gunnar, Laconi, Ezio, Wise, John Pierce, Nangami, Gladys, Marongiu, Fabio, Vaccari, Monica, Berg, Arthur, Jian, Le, Curran, Colleen S., Moon, Eun Yi, Wise, Sandra S., de Cerain Salsamendi, Adela Lopez, Yasaei, Hemad, Forte, Stefano, Krishnakumar, P. K., Thompson, Patricia, Zhang, Luoping, Raju, Jayadev, Lasfar, Ahmed, Palorini, Roberta, Mondello, Chiara, Romano, Simona, Memeo, Lorenzo, Andrade-Vieira, Rafaela, Salem, Hosni K., Karamouzis, Michalis V., Dent, Paul, Blanco-Aparicio, Carmen, Baglole, Carolyn J., Kondoh, Hiroshi, Wagemaker, Gerard, Decker, William K., Cheng, Qiang Shawn, Di Fiore, Riccardo, Hultman, Tove, Ali, Abdul Manaf, Kleinstreuer, Nicole, Luanpitpong, Sudjit, Gulliver, Linda, Salzberg, Anna C., D'Abronzo, Leandro S., Chapellier, Marion, Yedjou, Clement, Kirsch-Volders, Micheline, Guarnieri, Tiziana, Nahta, Rita, Ward, Andrew, Leyns, Luc, Cohen-Solal, Karine A., Hsu, Chia Wen, Lin, Liang Tzung, Chen, Tao, Corsini, Emanuela, Maguer-Satta, Veronique, Papagerakis, Silvana, Williams, Marc A., Roy, Rabindra, Williams, Graeme, Sinha, Ranjeet K., Roy, Debasish, Klaunig, James E., Singh, Neetu, Kuemmerle, Nancy B., Park, Hyun Ho, Barclay, Barry J., Bisson, William H., Vermeulen, Louis, Soucek, Laura, Khatami, Mahin, Sanderson, Thomas, Koppen, Gudrun, Darbre, Philippa, Ahmed, Nuzhat, Amedei, Amedeo, Dornetshuber-Fleiss, Rita, Al-Mulla, Fahd, Olsen, Ann Karin, Luqmani, Yunus, Hu, Zhiwei, Azqueta, Amaya, Leung, Po Sing, Moorwood, Kim, and Colacci, Annamaria
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1. No poverty ,3. Good health - Abstract
Lifestyle factors are responsible for a considerable portion of cancer incidence worldwide, but credible estimates from the World Health Organization and the International Agency for Research on Cancer (IARC) suggest that the fraction of cancers attributable to toxic environmental exposures is between 7% and 19%. To explore the hypothesis that low-dose exposures to mixtures of chemicals in the environment may be combining to contribute to environmental carcinogenesis, we reviewed 11 hallmark phenotypes of cancer, multiple priority target sites for disruption in each area and prototypical chemical disruptors for all targets, this included dose-response characterizations, evidence of low-dose effects and cross-hallmark effects for all targets and chemicals. In total, 85 examples of chemicals were reviewed for actions on key pathways/mechanisms related to carcinogenesis. Only 15% (13/85) were found to have evidence of a dose-response threshold, whereas 59% (50/85) exerted low-dose effects. No dose-response information was found for the remaining 26% (22/85). Our analysis suggests that the cumulative effects of individual (non-carcinogenic) chemicals acting on different pathways, and a variety of related systems, organs, tissues and cells could plausibly conspire to produce carcinogenic synergies. Additional basic research on carcinogenesis and research focused on low-dose effects of chemical mixtures needs to be rigorously pursued before the merits of this hypothesis can be further advanced. However, the structure of the World Health Organization International Programme on Chemical Safety ‘Mode of Action’ framework should be revisited as it has inherent weaknesses that are not fully aligned with our current understanding of cancer biology.
161. Glucose enzyme electrode with extended linearity
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Mullen, William H., primary, Keedy, Fiona H., additional, Churchouse, Stephen J., additional, and Vadgama, Pankaj M., additional
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- 1986
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162. An O2-based enzyme electrode for whole blood lactate measurement under continuous flow conditions
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Weaver, Mark R., primary and Vadgama, Pankaj M., additional
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- 1986
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163. Simplified urinary oxalate determination using an enzyme electrode
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Vadgama, Pankaj, primary, Sheldon, William, additional, Guy, John M., additional, Covington, Arthur K., additional, and Laker, Michael F., additional
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- 1984
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164. Characterization of thin films and membranes.
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Vadgama, Pankaj and Mandler, Daniel
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THIN films , *ARTIFICIAL membranes - Abstract
An introduction is presented in which the editors discuss reports within the issue on topics dedicated to the characterization of thin films and membranes.
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- 2013
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165. Challenges for successful implantation of biofuel cells.
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Zebda, Abdelkader, Alcaraz, Jean-Pierre, Vadgama, Pankaj, Shleev, Sergey, Minteer, Shelley D., Boucher, François, Cinquin, Philippe, and Martin, Donald K.
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ELECTROCHEMISTRY , *BIOCOMPATIBILITY , *BIOMEDICAL materials , *LITHIUM cells , *FOULING , *ARTIFICIAL implants - Abstract
Abstract There is a growing interest in the design and engineering of operational biofuel cells that can be implanted. This review highlights the recent progress in the electrochemistry of biofuel cell technologies, but with a particular emphasis on the medical and physiological aspects that impact the biocompatibility of biofuel cells operating inside a living body. We discuss the challenge of supplying power to implantable medical devices, with regard to the limitations of lithium battery technology and why implantable biofuel cells can be a promising alternative to provide the levels of power required for medical devices. In addition to the challenge of designing a biofuel cell that provides a stable level of sufficient power, the review highlights the biocompatibility and biofouling problems of implanting a biofuel cell that have a major impact on the availability of the substrates inside body that provide fuel for the biofuel cell. These physiological challenges and associated ethical considerations are essential to consider for biofuel cells that are designed to be implanted for long-term operation inside a living animal and eventually to human clinical applications. Highlights • Implanted biofuel cells (BFCs) have both electrochemical and biocompatibility needs. • Electrochemistry progress gives function of implanted BFC for several months. • Electrochemical challenges remain to optimise bioelectrode surface area and volume. • Biocompatibility challenges need to be overcome for long-term function in mammals. • Insight into optimising electrochemical and biocompatibility challenges is presented. [ABSTRACT FROM AUTHOR]
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- 2018
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166. Focus on sensor interfaces.
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Vadgama, Pankaj
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INTERFACES (Physical sciences) , *BIOSENSORS , *MEDICINE , *DETECTORS , *LIFE sciences - Abstract
The author discusses the importance of interfaces for sensing and the significance of their impact for the biological sciences and biomedicine. It is asserted that biosensors have provided notable examples of new interfacial processes. Sensor research is said to have led to the establishment of methods of integrating different interactive phases that combine to create the eventual device, thus leading to a refinement of interfaces.
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- 2010
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167. Editorial for special issue of medical engineering and physics
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Vadgama, Pankaj
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- 2006
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168. Self-cleaning sensors based on thermoresponsive polymeric film modified screen-printed platinum electrodes.
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Yang, Lei, Lopes, Ilanna Campelo, and Vadgama, Pankaj
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PLATINUM electrodes , *THERMORESPONSIVE polymers , *BIOLOGICAL interfaces , *ELECTROCHEMICAL sensors , *DETECTORS , *SURFACE contamination , *COLLOIDS - Abstract
[Display omitted] • P(NIPAAm-co-NVP) copolymer was successfully tethered to platinum paste electrodes. • Temperature addressable permeability change to H 2 O 2 and K 3 [Fe(CN) 6 ] was demonstrated. • Increased NVP content deduced high LCST to allow self-cleaning at body temperature. • Surface conformational remodeling was important for protein rejection. Electrochemical sensors have progressively become important tools for monitoring the concentration of complex substances in biological media. However, surface fouling and biological contamination from bio-colloids have not been effectively resolved. In this study, a self-cleaning electrochemical sensor was constructed where a thermoresponsive outer polymer layer was equipped to repel the absorbent proteins. Enhanced hydrophilic P(NIPAAm-co-NVP) copolymers bearing N-isopropylacrylamide (NIPAAm) and N-vinyl pyrrolidone (NVP) were synthesized by free radical polymerization, and the resulting thermoresponsive polymeric films were drop coated on screen-printed platinum electrodes (SPPEs) followed by thermal annealing. Grafting density, surface wettability, surface microstructure, amperometric response, electrochemical active surface area (EAS) and other performances, were evaluated for the modified SPPEs. Self-cleaning behavior after albumin adsorption was assessed by cyclic voltammetry (CV) and scanning electron microscopy (SEM) using various set temperatures respectively. The results showed the lower critical solution temperature (LCST) varied from 25 °C to 40 °C for P(NIPAAm-co-NVP) copolymers with the NVP content ranging from 0 to 47.62%. It indicated that NVP enhanced film hydrophilicity and could raise the LCST to above physiological data. The thermosensitivity, stability and repeatability of the modified sensors was proved to be well, and the unchanged selectivity was consistent with bare platinum electrodes. The copolymer film modified SPPEs showed the decreased and temperature-dependent EAS compared with bare SPPEs. It was concluded that films without NVP or with low NVP content resisted protein adsorption over lower temperatures, whilst with higher NVP content outstanding self-cleaning ability was also seen at physiological temperature. This opens the way to realize smart thermally addressable sensors able to solve the existing problem of biological surface contamination in sensor systems, and provides a strong model basis and technical capability for achieving novel, low drift electrochemical sensors. [ABSTRACT FROM AUTHOR]
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- 2023
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169. Preface
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Vadgama, Pankaj
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- 2005
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170. Microfluidic assays in practice.
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Vadgama, Pankaj
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BIOLOGICAL assay ,DRUG standards ,BIOLOGICAL reagents ,MICROFLUIDICS - Abstract
While microfluidics promises much for streamlining biological assays, the problem of sample deposition must first be solved. [Copyright &y& Elsevier]
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- 2006
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171. The characterization of liposomal glucose oxidase electrodes for the measurement of glucose
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Taylor, Mark A., Jones, Malcolm N., Vadgama, Pankaj M., and Higson, Séamus P.J.
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- 1995
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172. An O 2-based enzyme electrode for whole blood lactate measurement under continuous flow conditions
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Weaver, Mark R. and Vadgama, Pankaj M.
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- 1986
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173. Bioelectrochemical Determination of Citric Acid in Real Samples Using a Fully Automated Flow Injection Manifold.
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I. Prodromidis, Mamas, M. Tzouwara-Karayanni, Stella, I. Karayannis, Miltiades, and M. Vadgama, Pankaj
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- 1997
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174. Electrochemical Biosensing of Glucose Based on the Enzymatic Reduction of Glucose.
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Soranzo, Thomas, Ben Tahar, Awatef, Chmayssem, Ayman, Zelsmann, Marc, Vadgama, Pankaj, Lenormand, Jean-Luc, Cinquin, Phillipe, K. Martin, Donald, and Zebda, Abdelkader
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ALCOHOL dehydrogenase , *NICOTINAMIDE adenine dinucleotide phosphate , *GLUCOSE oxidase , *SORBITOL , *ALDOSE reductase , *GLUCOSE , *GOLD electrodes - Abstract
In this work, the enzyme aldehyde reductase, also known as aldose reductase, was synthesized and cloned from a human gene. Spectrophotometric measurements show that in presence of the nicotinamide adenine dinucleotide phosphate cofactor (NADPH), the aldehyde reductase catalyzed the reduction of glucose to sorbitol. Electrochemical measurements performed on an electrodeposited poly(methylene green)-modified gold electrode showed that in the presence of the enzyme aldehyde reductase, the electrocatalytic oxidation current of NADPH decreased drastically after the addition of glucose. These results demonstrate that aldehyde reductase is an enzyme that allows the construction of an efficient electrochemical glucose biosensor based on glucose reduction. [ABSTRACT FROM AUTHOR]
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- 2022
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175. Circadian Disruption and Remedial Interventions: Effects and Interventions for Jet Lag for Athletic Peak Performance.
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Forbes-Robertson, Sarah, Dudley, Edward, Vadgama, Pankaj, Cook, Christian, Drawer, Scott, and Kilduff, Liam
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PHYSIOLOGICAL adaptation , *AIR travel , *ATHLETIC ability , *CIRCADIAN rhythms , *DOPING in sports , *EXERCISE physiology , *INGESTION , *JET lag , *LIGHT , *MELATONIN , *PHOTOTHERAPY , *RUGBY football , *SUNSHINE , *TIME , *TRAVEL hygiene , *ATHLETIC associations , *PROFESSIONAL athletes , *PREVENTION - Abstract
Jet lag has potentially serious deleterious effects on performance in athletes following transmeridian travel, where time zones are crossed eastwards or westwards; as such, travel causes specific effects related to desynchronization of the athlete's internal body clock or circadian clock. Athletes are particularly sensitive to the effects ofjet lag, as many intrinsic aspects of sporting performance show a circadian rhythm, and optimum competitive results require all aspects of the athlete's mind and body to be working in tandem at their peak efficiency. International competition often requires transmeridian travel, and competition timings cannot be adjusted to suit individual athletes. It is therefore in the interest of the individual athlete and team to understand the effects of jet lag and the potential adaptation strategies that can be adopted. In this review, we describe the underlying genetic and physiological mechanisms controlling the circadian clock and its inherent ability to adapt to external conditions on a daily basis. We then examine the fundamentals of the various adaptation stimuli, such as light, chronobiotics (e.g. melatonin), exercise, and diet and meal timing, with particular emphasis on their suitability as strategies for competing athletes on the international circuit. These stimuli can be artificially manipulated to produce phase shifts in the circadian rhythm to promote adaptation in the optimum direction, but care must be taken to apply them at the correct time and dose, as the effects produced on the circadian rhythm follow a phase-response curve, with pronounced shifts in direction at different times. Light is the strongest realigning stimulus and careful timing of light exposure and avoidance can promote adjustment. Chronobiotics such as melatonin can also be used to realign the circadian clock but, as well as timing and dosage issues, there are also concerns as to its legal status in different countries and with the World Anti-Doping Agency. Experimental data concerning the effects of food intake and exercise timing on jet lag is limited to date in humans, and more research is required before firm guidelines can be stated. All these stimuli can also be used in pre-flight adaptation strategies to promote adjustment in the required direction, and implementation of these is described. In addition, the effects of individual variability at the behavioural and genetic levels are also discussed, along with the current limitations in assessment of these factors, and we then put forward three case studies, as examples of practical applications of these strategies, focusing on adaptations to travel involving competition in the Rugby Sevens World Cup and the 2016 Summer Olympics in Rio de Janeiro, Brazil. Finally, we provide a list of practice points for optimal adaptation of athletes to jet lag. [ABSTRACT FROM AUTHOR]
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- 2012
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176. Development of electrochemical biosensors based on sol-gel enzyme encapsulation and protective polymer membranes.
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Pauliukaite, Rasa, Schoenleber, Monika, Vadgama, Pankaj, and Brett, Christopher M. A.
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POLYMERS , *SURFACE coatings , *BIOSENSORS , *CHEMICAL detectors , *ENZYMES , *MICROENCAPSULATION , *BIOLOGICAL membranes - Abstract
Protective polymer coatings have been used to enhance the retention of enzymes in sol-gel films as immobilisation phases in electrochemical biosensors. Carbon film electrodes were electrochemically modified with poly(neutral red) (PNR). These electrodes were coated with oxysilane sol-gels incorporating glucose oxidase and an outer coating of carboxylated PVC (CPVC) or polyurethane (PU), with and without Aliquat-336 or isopropyl myristate (IPM) plasticizer, was applied. The biosensors were characterised electrochemically using cyclic voltammetry and amperometry, electrochemical impedance spectroscopy and scanning electron microscopy. Impedance spectra showed that the electrode surface is most active when the sol-gel–GOx layer is not covered with a membrane. However, membranes without plasticizer extend the lifetime of the biosensor to more than 2 months when PU is used as an outer membrane. The linear range of the biosensors was found to be 0.05–0.50 mM of glucose and the biosensor with PU outer membrane exhibited higher sensitivity (ca.117 nA mM−1) in the region of linear response than that with CPVC. The biosensors were applied to glucose measurement in natural samples of commercial orange juice. [ABSTRACT FROM AUTHOR]
- Published
- 2008
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177. Electrochemical Monitoring of Subcutaneous Tissue pO2 Fluctuations during Exercise Using a Semi‐implantable Needle Electrode.
- Author
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Anastasova, Salzitsa, Spehar‐Délèze, Anna‐Maria, Kwasnicki, Richard Mark, Yang, Guang‐Zhong, and Vadgama, Pankaj
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OXYGEN electrodes , *TRITON X-100 , *EXERCISE , *ELECTRODES , *BLOOD flow , *IMPLANTABLE cardioverter-defibrillators - Abstract
Semi‐implantable needle oxygen electrodes were used for forearm subcutaneous monitoring in human subjects undertaking high intensity cycling and fist clenching exercise. pO2 variations in the range between 40 and100 mm Hg oxygen were seen. Superimposed on these were paradoxical rises in subcutaneous pO2, of up to 100 mm Hg which paralleled the scale of the exercise. This was indicative of increased blood flow through skin. Triton X‐100 incorporated into the sensor polyurethane membranes helped to give faster responses and reduced the possibility of biofouling and drift. The sterilizable system, free from internal electrolyte film appears promising for future clinical monitoring. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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178. Bifunctional aptamer-mediated catalytic hairpin assembly for the sensitive and homogenous detection of rare cancer cells.
- Author
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Liu, Jumei, Zhang, Ye, Zhao, Qianwen, Situ, Bo, Zhao, Jiamin, Luo, Shihua, Li, Bo, Yan, Xiaohui, Vadgama, Pankaj, Su, Lei, Ma, Wen, Wang, Wen, and Zheng, Lei
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CANCER cells , *BODY fluid analysis , *METASTASIS , *HAIRPIN (Genetics) , *APTAMERS , *BIFUNCTIONAL catalysis , *FLUOROPHORES , *DIAGNOSIS - Abstract
The presence of cancer cells in body fluids confirms the occurrence of metastasis and guides treatment. A simple, fast, and homogeneous fluorescent method was developed to detect cancer cells based on catalytic hairpin assembly (CHA) and bifunctional aptamers. The bifunctional aptamer had a recognition domain for binding to target cancer cells and an initiator domain for triggering the CHA reaction. In the presence of target cells, the bifunctional aptamer was released from the inhibitor and initiated a cascade reaction of assembly and disassembly of the hairpins. Separation of the fluorophores from the quenchers produced fluorescence signals. The proposed strategy showed high specificity for discriminating normal cells and leukocytes, and the detection limit was 10 cells/mL, which was lower than that of previous aptasensors. This assay was further tested using four kinds of clinical samples spiked with target cells to confirm its applicability. We developed a simple, rapid, and cost-effective method for the detection of cancer cells that did not require purification, and the approach holds great potential for bioanalysis and early diagnosis. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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179. Electrochemical determination of microRNAs based on isothermal strand-displacement polymerase reaction coupled with multienzyme functionalized magnetic micro-carriers.
- Author
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Ma, Wen, Situ, Bo, Lv, Weifeng, Li, Bo, Yin, Xiaomao, Vadgama, Pankaj, Zheng, Lei, and Wang, Wen
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ELECTROCHEMICAL analysis , *MICRORNA , *POLYMERASE chain reaction , *MULTIENZYMES , *MAGNETIC fields , *MAGNETIC separation - Abstract
MicroRNAs (miRNAs) show great potential for disease diagnostics due to their specific molecular profiles. Detection of miRNAs remains challenging and often requires sophisticated platforms. Here we report a multienzyme-functionalized magnetic microcarriers-assisted isothermal strand-displacement polymerase reaction (ISDPR) for quantitative detection of miRNAs. Magnetic micro-carriers (MMCs) were functionalized with molecular beacons to enable miRNAs recognition and magnetic separation. The target miRNAs triggered a phi29-mediated ISDPR, which can produce biotin-modified sequences on the MMCs. Streptavidin–alkaline phosphatase was then conjugated to the MMC surface through biotin–streptavidin interactions. In the presence of 2-phospho- L -ascorbic acid, miRNAs were quantitatively determined on a screen-printed carbon electrode from the anodic current of the enzymatic product. We show that this method enables detection of miRNAs as low as 9 fM and allows the discrimination of one base mismatched sequence. The proposed method was also successfully applied to analyze miRNAs in clinical tumor samples. This paper reports a new strategy for miRNAs analysis with high sensitivity, simplicity, and low cost. It would be particularly useful for rapid point-of-care testing of miRNAs in clinical laboratory. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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180. The potential for chemical mixtures from the environment to enable the cancer hallmark of sustained proliferative signalling.
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Engström, Wilhelm, Darbre, Philippa, Eriksson, Staffan, Gulliver, Linda, Hultman, Tove, Karamouzis, Michalis V., Klaunig, James E., Mehta, Rekha, Moorwood, Kim, Sanderson, Thomas, Hideko Sone, Vadgama, Pankaj, Wagemaker, Gerard, Ward, Andrew, Singh, Neetu, Al-Mulla, Fahd, Al-Temaimi, Rabeah, Amedei, Amedeo, Colacci, Anna Maria, and Vaccari, Monica
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CHEMICAL carcinogenesis , *CELL proliferation , *TUMOR growth , *CELLULAR signal transduction , *BIOCHEMICAL mechanism of action - Abstract
The aim of this work is to review current knowledge relating the established cancer hallmark, sustained cell proliferation to the existence of chemicals present as low dose mixtures in the environment. Normal cell proliferation is under tight control, i.e. cells respond to a signal to proliferate, and although most cells continue to proliferate into adult life, the multiplication ceases once the stimulatory signal disappears or if the cells are exposed to growth inhibitory signals. Under such circumstances, normal cells remain quiescent until they are stimulated to resume further proliferation. In contrast, tumour cells are unable to halt proliferation, either when subjected to growth inhibitory signals or in the absence of growth stimulatory signals. Environmental chemicals with carcinogenic potential may cause sustained cell proliferation by interfering with some cell proliferation control mechanisms committing cells to an indefinite proliferative span. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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181. Chemosensors and biosensors based on polyelectrolyte microcapsules containing fluorescent dyes and enzymes.
- Author
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Kazakova, Lyubov, Shabarchina, Lyudmila, Anastasova, Salzitsa, Pavlov, Anton, Vadgama, Pankaj, Skirtach, Andre, and Sukhorukov, Gleb
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ENZYMES , *FLUORESCENCE , *POLYMERS , *PROTEINS , *CALCIUM carbonate , *HYDROGEN peroxide - Abstract
The concept of enzyme-assisted substrate sensing based on use of fluorescent markers to detect the products of enzymatic reaction has been investigated by fabrication of micron-scale polyelectrolyte capsules containing enzymes and dyes in one entity. Microcapsules approximately 5 μm in size entrap glucose oxidase or lactate oxidase, with peroxidase, together with the corresponding markers Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride (Ru(dpp)) complex and dihydrorhodamine 123 (DHR123), which are sensitive to oxygen and hydrogen peroxide, respectively. These capsules are produced by co-precipitation of calcium carbonate particles with the enzyme followed by layer-by-layer assembly of polyelectrolytes over the surface of the particles and incorporation of the dye in the capsule interior or in the multilayer shell. After dissolution of the calcium carbonate the enzymes and dyes remain in the multilayer capsules. In this study we produced enzyme-containing microcapsules sensitive to glucose and lactate. Calibration curves based on fluorescence intensity of Ru(dpp) and DHR123 were linearly dependent on substrate concentration, enabling reliable sensing in the millimolar range. The main advantages of using these capsules with optical recording is the possibility of building single capsule-based sensors. The response from individual capsules was observed by confocal microscopy as increasing fluorescence intensity of the capsule on addition of lactate at millimolar concentrations. Because internalization of the micron-sized multi-component capsules was feasible, they could be further optimized for in-situ intracellular sensing and metabolite monitoring on the basis of fluorescence reporting. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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182. Effective diffusion coefficient determination within cylindrical granules of adsorbents using a direct simulation method
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Rong, Zimei, Terzyk, Artur P., Gauden, Piotr A., and Vadgama, Pankaj
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ADSORPTION (Chemistry) , *SEPARATION (Technology) , *SURFACE chemistry , *SIMULATION methods & models - Abstract
Abstract: Analytical expressions for solute adsorption kinetics within porous carbon cylindrical granules of adsorbents with a one point formula for effective diffusion coefficient determination are available based on the assumption that solute transport is the rate limiting step and that it follows Fick''s Second Law. Here the first practical application of this theory is provided with an initial, estimated diffusion coefficient refined by fitting calculated kinetic adsorption curves to experimental data determined for activated carbons. In an ideal experiment, experimental error (noise) is negligible, and no data refinement is needed. However, real experimental data are always more or less noise contaminated. Where such noise is significant, a simulation method offers the best value for effective diffusion coefficient. For this specific system, surface modification, pH and temperature effects on adsorption kinetics were analysed quantitatively as a basis of determining effective diffusion coefficients through the porous structure. [Copyright &y& Elsevier]
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- 2007
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183. Spider and mulberry silkworm silks as compatible biomaterials
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Hakimi, Osnat, Knight, David P., Vollrath, Fritz, and Vadgama, Pankaj
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FIBERS , *BIOMECHANICS , *PLANT mechanics , *SILK - Abstract
Abstract: Silks are a diverse family of natural materials with extraordinary mechanical properties such as high tensile strength and extensibility, as well as reported biological compatibility. In recent years, the reported exceptional nature of silk lead to increased interest in silk for biomedical applications. The aim of this review is to assess the potential and compatibility of silk fibres and silk-based materials for biomedical purposes. It will do so by reviewing silk properties, structure and formation, with special focus on spider and mulberry silkworm silk fibres, as well as the application of silk in the biomedical field. The review will begin by introducing the general characteristics of silk, and a consideration of properties of particular relevance to the use of silk in biomedical applications: degradation and tensile properties. Subsequently, the formation of silk in vivo will be outlined, as well as the current understanding of silk structure. A comparison of the structural differences between spider and silkworm silks will follow. Some of the different types of silk produced by orb weaving spiders, their main functions and structural features will be described. This will be followed by an introduction to ‘supercontraction’, a phenomenon that has only been observed in spider silks, and is considered to be one of the major obstacles to the use of native spider silk for medical applications. Finally, there will be an account of previous biomedical applications of silk. It is the intention of this review to point out the wide range of excellent and valuable properties observed in different silk types, and to propose that silk’s versatility is possibly its strongest advantage. However, in the context of biomedical research and development, there are still major limitations and difficulties with native silk fibres. It is suggested in this review that an artificially produced silk or silk-like material formed to possess specific desired properties will allow the overcoming of present limitations. [Copyright &y& Elsevier]
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- 2007
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184. Characterisation of the nanoporous structure of collagen-glycosaminoglycan hydrogels by freezing-out of bulk and bound water
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Mikhalovska, Lyuba I., Gun’ko, Vlad M., Turov, Vlad V., Zarko, Vlad I., James, Stuart L., Vadgama, Pankaj, Tomlins, Paul E., and Mikhalovsky, Sergey Victorovich
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HYDROGELS , *NUCLEAR magnetic resonance spectroscopy , *BIOPOLYMERS , *BIOMEDICAL materials - Abstract
Abstract: The nanoporous structure of collagen-glycosaminoglycan (CG) hydrogels was studied using 1H NMR spectroscopy and thermally stimulated depolarisation (TSD) current with layer-by-layer freezing-out of bulk and interfacial water. The depression of the freezing point of water is related to the size of the nanopore, to which it is confined. Changes in the Gibbs free energy of the unfrozen interfacial water are related to the amount of bound water in the hydrogel matrix and to the re-arrangement of the 3D network structure of the biopolymer. Analysis of the thermodynamic properties of bulk and interfacial water using the layer-by-layer freezing-out technique combined with NMR and TSDC provides valuable information about the structural features of CG hydrogels that can be used for characterisation of different types of hydrogels and soft tissue scaffolds, artificial skin substitutes and other biomaterials. [Copyright &y& Elsevier]
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- 2006
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185. Voltammetric and impedance studies of inosine-5′-monophosphate and hypoxanthine
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Oliveira-Brett, Ana Maria, Silva, Luís A., Farace, Giosi, Vadgama, Pankaj, and Brett, Christopher M.A.
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INOSINE , *ELECTROCHEMISTRY - Abstract
The oxidation mechanism and adsorption of inosine 5′-monophosphate and hypoxanthine were investigated in solutions of different pH using voltammetric and impedance methods at glassy carbon electrodes. For both compounds, the pH dependence from differential pulse voltammetry showed that the same number of electrons and protons are involved in the rate-determining step of the electrochemical reaction. In the case of hypoxanthine, it was also possible to study the effect of different concentrations. At high concentrations of hypoxanthine, two oxidation peaks were observed, the first due to hypoxanthine oxidation with formation of oligomers and the second due to hypoxanthine oligomer oxidation, both compounds adsorbing strongly. Impedance measurements corroborated the voltammetric results and enabled the study of the adsorption of hypoxanthine on glassy carbon. [Copyright &y& Elsevier]
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- 2003
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186. Comparison of Dual Beam Dispersive and FTNIR Spectroscopy for Lactate Detection.
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Baishya, Nystha, Mamouei, Mohammad, Budidha, Karthik, Qassem, Meha, Vadgama, Pankaj, Kyriacou, Panayiotis A., and Fakayode, Sayo O.
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LACTATES , *SPECTROMETRY , *NEAR infrared spectroscopy - Abstract
Near Infrared (800–2500 nm) spectroscopy has been extensively used in biomedical applications, as it offers rapid, in vivo, bed-side monitoring of important haemodynamic parameters, which is especially important in critical care settings. However, the choice of NIR spectrometer needs to be investigated for biomedical applications, as both the dual beam dispersive spectrophotomer and the FTNIR spectrometer have their own advantages and disadvantages. In this study, predictive analysis of lactate concentrations in whole blood were undertaken using multivariate techniques on spectra obtained from the two spectrometer types simultaneously and results were compared. Results showed significant improvement in predicting analyte concentration when analysis was performed on full range spectral data. This is in comparison to analysis of limited spectral regions or lactate signature peaks, which yielded poorer prediction models. Furthermore, for the same region, FTNIR showed 10% better predictive capability than the dual beam dispersive NIR spectrometer. [ABSTRACT FROM AUTHOR]
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- 2021
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187. Assessing the carcinogenic potential of low-dose exposures to chemical mixtures in the environment: the challenge ahead
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Dustin G. Brown, Tove Hultman, Judith Weisz, H. Kim Lyerly, Paola A. Marignani, Ann-Karin Olsen, Rabindra Roy, Kim Moorwood, Masoud H. Manjili, Monica Vaccari, Jesse Roman, Hasiah Ab Hamid, Kalan R. Prudhomme, Periyadan K. Krishnakumar, Chenfang Dong, Tiziana Guarnieri, Leandro S. D'Abronzo, Gloria M. Calaf, Amelia K Charles, Emanuela Corsini, Yunus A. Luqmani, Graeme Williams, Louis Vermeulen, Pankaj Vadgama, Sarah N Bay, Véronique Maguer-Satta, Sabine A. S. Langie, Christian C. Naus, Le Jian, Gladys N. Nangami, Lorenzo Memeo, Stephanie C. Casey, Thomas Sanderson, Takemi Otsuki, Nichola Cruickshanks, William H. Bisson, Sudjit Luanpitpong, Jonathan Whitfield, Ahmed Lasfar, Yon Rojanasakul, A. Ivana Scovassi, Shelley A. Harris, Ferdinando Chiaradonna, Richard Ponce-Cusi, Gregory T. Wolf, Valérian Dormoy, Roslida Abd Hamid, Hyun Ho Park, Matilde E. Lleonart, William K. Decker, Maria Romano, Leroy Lowe, Fabio Marongiu, Jan Vondráček, Chiara Mondello, Luc Leyns, Josiah Ochieng, Pratima Nangia-Makker, Edward A. Ratovitski, Zhiwei Hu, Jayadev Raju, Hemad Yasaei, Rafaela Andrade-Vieira, Jordan Woodrick, Hideko Sone, Harini Krishnan, W. Kimryn Rathmell, Andrew Collins, Luoping Zhang, Barry J. Barclay, Amaya Azqueta, Laura Soucek, Marc A. Williams, David O. Carpenter, Roberta Palorini, Rita Nahta, Juan Fernando Martinez-Leal, Firouz Darroudi, Rita Dornetshuber-Fleiss, James E. Klaunig, Elizabeth P. Ryan, Qiang Shawn Cheng, Arthur Berg, Andrew Ward, Gudrun Koppen, Tao Chen, Petr Heneberg, Michael Gilbertson, Amedeo Amedei, Sakina E. Eltom, Ezio Laconi, Joseph Christopher, Hiroshi Kondoh, Neetu Singh, Danielle J Carlin, Marion Chapellier, Michalis V. Karamouzis, Rekha Mehta, Tae-Jin Lee, Annamaria Colacci, Venkata S. Sabbisetti, Mark Wade, Micheline Kirsch-Volders, Patricia Ostrosky-Wegman, Isabelle R. Miousse, Patricia A. Thompson, Philippa D. Darbre, Frederik J. van Schooten, Sofia Pavanello, Igor Koturbash, Binhua P. Zhou, Ranjeet Kumar Sinha, Anna C. Salzberg, Mahara Valverde, Fahd Al-Mulla, Julia Kravchenko, Nicole Kleinstreuer, Carolyn J. Baglole, Menghang Xia, Samira A. Brooks, Amancio Carnero, Gunnar Brunborg, Sandra S. Wise, Daniel C. Koch, John Pierce Wise, Rabeah Al-Temaimi, Laetitia Gonzalez, Lisa J. McCawley, R. Brooks Robey, Gary S. Goldberg, Thierry Massfelder, Linda S M Gulliver, Olugbemiga Ogunkua, Emilio Rojas, Eun-Yi Moon, Lin Li, Silvana Papagerakis, Nik van Larebeke, Adela Lopez de Cerain Salsamendi, Staffan Eriksson, Simona Romano, Dean W. Felsher, Paramita M. Ghosh, Karine A. Cohen-Solal, Paul Dent, Jun Sun, Carmen Blanco-Aparicio, Riccardo Di Fiore, Chia-Wen Hsu, Mahin Khatami, Kannan Badri Narayanan, Francis Martin, Colleen S. Curran, Dale W. Laird, William H. Goodson, Abdul Manaf Ali, Valerie Odero-Marah, Michael J. Gonzalez, Renza Vento, Liang Tzung Lin, Clement G. Yedjou, Hosni Salem, Hsue-Yin Hsu, Zhenbang Chen, Nuzhat Ahmed, Gerard Wagemaker, Sandra Ryeom, Stefano Forte, Debasish Roy, Nancy B. Kuemmerle, Robert C. Castellino, Po Sing Leung, Wilhelm Engström, National Institute of Environmental Health Sciences (US), Research Council of Norway, Ministerio de Economía y Competitividad (España), Instituto de Salud Carlos III, Red Temática de Investigación Cooperativa en Cáncer (España), European Commission, Junta de Andalucía, Ministerio de Educación y Ciencia (España), Ministero dell'Istruzione, dell'Università e della Ricerca, University of Oslo, Regione Emilia Romagna, National Institutes of Health (US), Consejo Nacional de Ciencia y Tecnología (México), Associazione Italiana per la Ricerca sul Cancro, National Research Foundation of Korea, Ministry of Education, Science and Technology (South Korea), Fondo Nacional de Desarrollo Científico y Tecnológico (Chile), Ministry of Education, Culture, Sports, Science and Technology (Japan), Japan Science and Technology Agency, Ministry of Science and Technology (Taiwan), Arkansas Biosciences Institute, Czech Science Foundation, Fundación Fero, Swim Across America, American Cancer Society, Research Foundation - Flanders, Austrian Science Fund, Institut National de la Santé et de la Recherche Médicale (France), Natural Sciences and Engineering Research Council of Canada, Farmacologie en Toxicologie, RS: NUTRIM - R4 - Gene-environment interaction, Goodson, William H, Lowe, Leroy, Carpenter, David O, Gilbertson, Michael, Manaf Ali, Abdul, Lopez de Cerain Salsamendi, Adela, Lasfar, Ahmed, Carnero, Amancio, Azqueta, Amaya, Amedei, Amedeo, Charles, Amelia K, Collins, Andrew R, Ward, Andrew, Salzberg, Anna C, Colacci, Annamaria, Olsen, Ann Karin, Berg, Arthur, Barclay, Barry J, Zhou, Binhua P, Blanco Aparicio, Carmen, Baglole, Carolyn J, Dong, Chenfang, Mondello, Chiara, Hsu, Chia Wen, Naus, Christian C, Yedjou, Clement, Curran, Colleen S, Laird, Dale W, Koch, Daniel C, Carlin, Danielle J, Felsher, Dean W, Roy, Debasish, Brown, Dustin G, Ratovitski, Edward, Ryan, Elizabeth P, Corsini, Emanuela, Rojas, Emilio, Moon, Eun Yi, Laconi, Ezio, Marongiu, Fabio, Al Mulla, Fahd, Chiaradonna, Ferdinando, Darroudi, Firouz, Martin, Francis L, Van Schooten, Frederik J, Goldberg, Gary S, Wagemaker, Gerard, Nangami, Gladys N, Calaf, Gloria M, Williams, Graeme, Wolf, Gregory T, Koppen, Gudrun, Brunborg, Gunnar, Lyerly, H. Kim, Krishnan, Harini, Ab Hamid, Hasiah, Yasaei, Hemad, Sone, Hideko, Kondoh, Hiroshi, Salem, Hosni K, Hsu, Hsue Yin, Park, Hyun Ho, Koturbash, Igor, Miousse, Isabelle R, Scovassi, A. Ivana, Klaunig, James E, Vondráček, Jan, Raju, Jayadev, Roman, Jesse, Wise, John Pierce, Whitfield, Jonathan R, Woodrick, Jordan, Christopher, Joseph A, Ochieng, Josiah, Martinez Leal, Juan Fernando, Weisz, Judith, Kravchenko, Julia, Sun, Jun, Prudhomme, Kalan R, Narayanan, Kannan Badri, Cohen Solal, Karine A, Moorwood, Kim, Gonzalez, Laetitia, Soucek, Laura, Jian, Le, D'Abronzo, Leandro S, Lin, Liang Tzung, Li, Lin, Gulliver, Linda, Mccawley, Lisa J, Memeo, Lorenzo, Vermeulen, Loui, Leyns, Luc, Zhang, Luoping, Valverde, Mahara, Khatami, Mahin, Romano, MARIA FIAMMETTA, Chapellier, Marion, Williams, Marc A, Wade, Mark, Manjili, Masoud H, Lleonart, Matilde E, Xia, Menghang, Gonzalez, Michael J, Karamouzis, Michalis V, Kirsch Volders, Micheline, Vaccari, Monica, Kuemmerle, Nancy B, Singh, Neetu, Cruickshanks, Nichola, Kleinstreuer, Nicole, van Larebeke, Nik, Ahmed, Nuzhat, Ogunkua, Olugbemiga, Krishnakumar, P. K, Vadgama, Pankaj, Marignani, Paola A, Ghosh, Paramita M, Ostrosky Wegman, Patricia, Thompson, Patricia A, Dent, Paul, Heneberg, Petr, Darbre, Philippa, Sing Leung, Po, Nangia Makker, Pratima, Cheng, Qiang Shawn, Robey, R. Brook, Al Temaimi, Rabeah, Roy, Rabindra, Andrade Vieira, Rafaela, Sinha, Ranjeet K, Mehta, Rekha, Vento, Renza, Di Fiore, Riccardo, Ponce Cusi, Richard, Dornetshuber Fleiss, Rita, Nahta, Rita, Castellino, Robert C, Palorini, Roberta, Abd Hamid, Roslida, Langie, Sabine A. S, Eltom, Sakina E, Brooks, Samira A, Ryeom, Sandra, Wise, Sandra S, Bay, Sarah N, Harris, Shelley A, Papagerakis, Silvana, Romano, Simona, Pavanello, Sofia, Eriksson, Staffan, Forte, Stefano, Casey, Stephanie C, Luanpitpong, Sudjit, Lee, Tae Jin, Otsuki, Takemi, Chen, Tao, Massfelder, Thierry, Sanderson, Thoma, Guarnieri, Tiziana, Hultman, Tove, Dormoy, Valérian, Odero Marah, Valerie, Sabbisetti, Venkata, Maguer Satta, Veronique, Rathmell, W. Kimryn, Engström, Wilhelm, Decker, William K, Bisson, William H, Rojanasakul, Yon, Luqmani, Yunu, Chen, Zhenbang, Hu, Zhiwei, Goodson, W., Lowe, L., Carpenter, D., Gilbertson, M., Ali, A., de Cerain Salsamendi, A., Lasfar, A., Carnero, A., Azqueta, A., Amedei, A., Charles, A., Collins, A., Ward, A., Salzberg, A., Colacci, A., Olsen, A., Berg, A., Barclay, B., Zhou, B., Blanco-Aparicio, C., Baglole, C., Dong, C., Mondello, C., Hsu, C., Naus, C., Yedjou, C., Curran, C., Laird, D., Koch, D., Carlin, D., Felsher, D., Roy, D., Brown, D., Ratovitski, E., Ryan, E., Corsini, E., Rojas, E., Moon, E., Laconi, E., Marongiu, F., Al-Mulla, F., Chiaradonna, F., Darroudi, F., Martin, F., Van Schooten, F., Goldberg, G., Wagemaker, G., Nangami, G., Calaf, G., Williams, G., Wolf, G., Koppen, G., Brunborg, G., Kim Lyerly, H., Krishnan, H., Hamid, H., Yasaei, H., Sone, H., Kondoh, H., Salem, H., Hsu, H., Park, H., Koturbash, I., Miousse, I., Ivana Scovassi, A., Klaunig, J., Vondráček, J., Raju, J., Roman, J., Wise, J., Whitfield, J., Woodrick, J., Christopher, J., Ochieng, J., Martinez-Leal, J., Weisz, J., Kravchenko, J., Sun, J., Prudhomme, K., Narayanan, K., Cohen-Solal, K., Moorwood, K., Gonzalez, L., Soucek, L., Jian, L., D'Abronzo, L., Lin, L., Li, L., Gulliver, L., Mccawley, L., Memeo, L., Vermeulen, L., Leyns, L., Zhang, L., Valverde, M., Khatami, M., Romano, M., Chapellier, M., Williams, M., Wade, M., Manjili, M., Lleonart, M., Xia, M., Gonzalez, M., Karamouzis, M., Kirsch-Volders, M., Vaccari, M., Kuemmerle, N., Singh, N., Cruickshanks, N., Kleinstreuer, N., Van Larebeke, N., Ahmed, N., Ogunkua, O., Krishnakumar, P., Vadgama, P., Marignani, P., Ghosh, P., Ostrosky-Wegman, P., Thompson, P., Dent, P., Heneberg, P., Darbre, P., Leung, P., Nangia-Makker, P., Cheng, Q., Brooks Robey, R., Al-Temaimi, R., Roy, R., Andrade-Vieira, R., Sinha, R., Mehta, R., Vento, R., Di Fiore, R., Ponce-Cusi, R., Dornetshuber-Fleiss, R., Nahta, R., Castellino, R., Palorini, R., Hamid, R., Langie, S., Eltom, S., Brooks, S., Ryeom, S., Wise, S., Bay, S., Harris, S., Papagerakis, S., Romano, S., Pavanello, S., Eriksson, S., Forte, S., Casey, S., Luanpitpong, S., Lee, T., Otsuki, T., Chen, T., Massfelder, T., Sanderson, T., Guarnieri, T., Hultman, T., Dormoy, V., Odero-Marah, V., Sabbisetti, V., Maguer-Satta, V., Kimryn Rathmell, W., Engström, W., Decker, W., Bisson, W., Rojanasakul, Y., Luqmani, Y., Chen, Z., Hu, Z., Goodson, W.H., Carpenter, D.O., Ali, A.M., de Cerain Salsamendi, A.L., Charles, A.K., Collins, A.R., Salzberg, A.C., Olsen, A.-K., Barclay, B.J., Zhou, B.P., Baglole, C.J., Hsu, C.-W., Naus, C.C., Curran, C.S., Laird, D.W., Koch, D.C., Carlin, D.J., Felsher, D.W., Brown, D.G., Ryan, E.P., Moon, E.-Y., Martin, F.L., Van Schooten, F.J., Goldberg, G.S., Calaf, G.M., Wolf, G.T., Hamid, H.A., Salem, H.K., Hsu, H.-Y., Park, H.H., Miousse, I.R., Klaunig, J.E., Vondracek, J., Wise, J.P., Whitfield, J.R., Christopher, J.A., Martinez-Leal, J.F., Prudhomme, K.R., Narayanan, K.B., Cohen-Solal, K.A., D'Abronzo, L.S., Lin, L.-T., Mccawley, L.J., Romano, M.F., Williams, M.A., Manjili, M.H., Gonzalez, M.J., Karamouzis, M.V., Kuemmerle, N.B., Krishnakumar, P.K., Marignani, P.A., Ghosh, P.M., Leung, P.S., Cheng, Q.S., Sinha, R.K., Castellino, R.C., Hamid, R.A., Langie, S.A.S., Brooks, S.A., Wise, S.S., Bay, S.N., Harris, S.A., Casey, S.C., Lee, T.-J., Engstrom, W., Decker, W.K., Bisson, W.H., sans affiliation, Centre de Recherche en Cancérologie de Lyon (UNICANCER/CRCL), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA), Institut Armand Frappier (INRS-IAF), Institut National de la Recherche Scientifique [Québec] (INRS)-Réseau International des Instituts Pasteur (RIIP), We gratefully acknowledge the support of the National Institute of Health-National Institute of Environmental Health Sciences (NIEHS) conference grant travel support (R13ES023276), Glenn Rice, Office of Research and Development, United States Environmental Protection Agency, Cincinnati, OH, USA also deserves thanks for his thoughtful feedback and inputs on the manuscript, William H.Goodson III was supported by the California Breast Cancer Research Program, Clarence Heller Foundation and California Pacific Medical Center Foundation, Abdul M.Ali would like to acknowledge the financial support of the University of Sultan Zainal Abidin, Malaysia, Ahmed Lasfar was supported by an award from the Rutgers Cancer Institute of New Jersey, Ann-Karin Olsen and Gunnar Brunborg were supported by the Research Council of Norway (RCN) through its Centres of Excellence funding scheme (223268/F50), Amancio Carnero’s lab was supported by grants from the Spanish Ministry of Economy and Competitivity, ISCIII (Fis: PI12/00137, RTICC: RD12/0036/0028) co-funded by FEDER from Regional Development European Funds (European Union), Consejeria de Ciencia e Innovacion (CTS-1848) and Consejeria de Salud of the Junta de Andalucia (PI-0306-2012), Matilde E. Lleonart was supported by a trienal project grant PI12/01104 and by project CP03/00101 for personal support. Amaya Azqueta would like to thank the Ministerio de Educacion y Ciencia (‘Juande la Cierva’ programme, 2009) of the Spanish Government for personal support, Amedeo Amedei was supported by the Italian Ministry of University and Research (2009FZZ4XM_002), and the University of Florence (ex60%2012), Andrew R.Collins was supported by the University of Oslo, Annamaria Colacci was supported by the Emilia-Romagna Region - Project ‘Supersite’ in Italy, Carolyn Baglole was supported by a salary award from the Fonds de recherche du Quebec-Sante (FRQ-S), Chiara Mondello’s laboratory is supported by Fondazione Cariplo in Milan, Italy (grant n. 2011-0370), Christian C.Naus holds a Canada Research Chair, Clement Yedjou was supported by a grant from the National Institutes of Health (NIH-NIMHD grant no. G12MD007581), Daniel C.Koch is supported by the Burroughs Wellcome Fund Postdoctoral Enrichment Award and the Tumor Biology Training grant: NIH T32CA09151, Dean W. Felsher would like to acknowledge the support of United States Department of Health and Human Services, NIH grants (R01 CA170378 PQ22, R01 CA184384, U54 CA149145, U54 CA151459, P50 CA114747 and R21 CA169964), Emilio Rojas would like to thank CONACyT support 152473, Ezio Laconi was supported by AIRC (Italian Association for Cancer Research, grant no. IG 14640) and by the Sardinian Regional Government (RAS), Eun-Yi Moon was supported by grants from the Public Problem-Solving Program (NRF-015M3C8A6A06014500) and Nuclear R&D Program (#2013M2B2A9A03051296 and 2010-0018545) through the National Research Foundation of Korea (NRF) and funded by the Ministry of Education, Science and Technology (MEST) in Korea, Fahd Al-Mulla was supported by the Kuwait Foundation for the Advancement of Sciences (2011-1302-06), Ferdinando Chiaradonna is supported by SysBioNet, a grant for the Italian Roadmap of European Strategy Forum on Research Infrastructures (ESFRI) and by AIRC (Associazione Italiana Ricerca sul Cancro, IG 15364), Francis L.Martin acknowledges funding from Rosemere Cancer Foundation, he also thanks Lancashire Teaching Hospitals NHS trust and the patients who have facilitated the studies he has undertaken over the course of the last 10 years, Gary S.Goldberg would like to acknowledge the support of the New Jersey Health Foundation, Gloria M.Calaf was supported by Fondo Nacional de Ciencia y Tecnología (FONDECYT), Ministerio de Educación de Chile (MINEDUC), Universidad de Tarapacá (UTA), Gudrun Koppen was supported by the Flemish Institute for Technological Research (VITO), Belgium, Hemad Yasaei was supported from a triennial project grant (Strategic Award) from the National Centre for the Replacement, Refinement and Reduction (NC3Rs) of animals in research (NC.K500045.1 and G0800697), Hiroshi Kondoh was supported in part by grants from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, Japan Science and Technology Agency and by JST, CREST, Hsue-Yin Hsu was supported by the Ministry of Science and Technology of Taiwan (NSC93-2314-B-320-006 and NSC94-2314-B-320-002), Hyun Ho Park was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) of the Ministry of Education, Science and Technology (2012R1A2A2A01010870) and a grant from the Korea Healthcare Technology R&D project, Ministry of Health and Welfare, Republic of Korea (HI13C1449), Igor Koturbash is supported by the UAMS/NIH Clinical and Translational Science Award (UL1TR000039 and KL2TR000063) and the Arkansas Biosciences Institute, the major research component of the Arkansas Tobacco Settlement Proceeds Act of 2000, Jan Vondráček acknowledges funding from the Czech Science Foundation (13-07711S), Jesse Roman thanks the NIH for their support (CA116812), John Pierce Wise Sr. and Sandra S.Wise were supported by National Institute of Environmental Health Sciences (ES016893 to J.P.W.) and the Maine Center for Toxicology and Environmental Health, Jonathan Whitfield acknowledges support from the FERO Foundation in Barcelona, Spain, Joseph Christopher is funded by Cancer Research UK and the International Journal of Experimental Pathology, Julia Kravchenko is supported by a philanthropic donation by Fred and Alice Stanback, Jun Sun is supported by a Swim Across America Cancer Research Award, Karine A.Cohen-Solal is supported by a research scholar grant from the American Cancer Society (116683-RSG-09-087-01-TBE), Laetitia Gonzalez received a postdoctoral fellowship from the Fund for Scientific Research–Flanders (FWO-Vlaanderen) and support by an InterUniversity Attraction Pole grant (IAP-P7-07), Laura Soucek is supported by grant #CP10/00656 from the Miguel Servet Research Contract Program and acknowledges support from the FERO Foundation in Barcelona, Spain, Liang-Tzung Lin was supported by funding from the Taipei Medical University (TMU101-AE3-Y19), Linda Gulliver is supported by a Genesis Oncology Trust (NZ) Professional Development Grant, and the Faculty of Medicine, University of Otago, Dunedin, New Zealand, Louis Vermeulen is supported by a Fellowship of the Dutch Cancer Society (KWF, UVA2011-4969) and a grant from the AICR (14–1164), Mahara Valverde would like to thank CONACyT support 153781, Masoud H. Manjili was supported by the office of the Assistant Secretary of Defense for Health Affairs (USA) through the Breast Cancer Research Program under Award No. W81XWH-14-1-0087 Neetu Singh was supported by grant #SR/FT/LS-063/2008 from the Department of Science and Technology, Government of India, Nicole Kleinstreuer is supported by NIEHS contracts (N01-ES 35504 and HHSN27320140003C), P.K. Krishnakumar is supported by the Funding (No. T.K. 11-0629) of King Abdulaziz City for Science and Technology, Riyadh, Saudi Arabia, Paola A.Marignani is supported by the Dalhousie Medical Research Foundation, The Beatrice Hunter Cancer Institute and CIHR and the Nova Scotia Lung Association, Paul Dent is the holder of the Universal Inc.Chair in Signal Transduction Research and is supported with funds from PHS grants from the NIH (R01-CA141704, R01-CA150214, R01-DK52825 and R01-CA61774), Petr Heneberg was supported by the Charles University in Prague projects UNCE 204015 and PRVOUK P31/2012, and by the Czech Science Foundation projects P301/12/1686 and 15-03834Y, Po Sing Leung was supported by the Health and Medical Research Fund of Food and Health Bureau, Hong Kong Special Administrative Region, Ref. No: 10110021, Qiang Cheng was supported in part by grant NSF IIS-1218712, R. Brooks Robey is supported by the United States Department of Veterans Affairs, Rabindra Roy was supported by United States Public Health Service Grants (RO1 CA92306, RO1 CA92306-S1 and RO1 CA113447), Rafaela Andrade-Vieira is supported by the Beatrice Hunter Cancer Research Institute and the Nova Scotia Health Research Foundation, Renza Vento was partially funded by European Regional Development Fund, European Territorial Cooperation 2007–13 (CCI 2007 CB 163 PO 037, OP Italia-Malta 2007–13) and grants from the Italian Ministry of Education, University and Research (MIUR) ex-60%, 2007, Riccardo Di Fiore was a recipient of fellowship granted by European Regional Development Fund, European Territorial Cooperation 2007–2013 (CCI 2007 CB 163 PO 037, OP Italia-Malta 2007–2013), Rita Dornetshuber-Fleiss was supported by the Austrian Science Fund (FWF, project number T 451-B18) and the Johanna Mahlke, geb.-Obermann-Stiftung, Roberta Palorini is supported by a SysBioNet fellowship, Roslida Abd Hamid is supported by the Ministry of Education, Malaysia-Exploratory Research Grant Scheme-Project no: ERGS/1-2013/5527165, Sabine A.S.Langie is the beneficiary of a postdoctoral grant from the AXA Research Fund and the Cefic-LRI Innovative Science Award 2013, Sakina Eltom is supported by NIH grant SC1CA153326, Samira A.Brooks was supported by National Research Service Award (T32 ES007126) from the National Institute of Environmental Health Sciences and the HHMI Translational Medicine Fellowship, Sandra Ryeom was supported by The Garrett B. Smith Foundation and the TedDriven Foundation, Thierry Massfelder was supported by the Institut National de la Santé et de la Recherche Médicale INSERM and Université de Strasbourg, Thomas Sanderson is supported by the Canadian Institutes of Health Research (CIHR, MOP-115019), the Natural Sciences and Engineering Council of Canada (NSERC, 313313) and the California Breast Cancer Research Program (CBCRP, 17UB-8703), Tiziana Guarnieri is supported by a grant from Fundamental Oriented Research (RFO) to the Alma Mater Studiorum University of Bologna, Bologna, Italy and thanks the Fondazione Cassa di Risparmio di Bologna and the Fondazione Banca del Monte di Bologna e Ravenna for supporting the Center for Applied Biomedical Research, S.Orsola-Malpighi University Hospital, Bologna, Italy, W.Kimryn Rathmell is supported by the V Foundation for Cancer Research and the American Cancer Society, William K.Decker was supported in part by grant RP110545 from the Cancer Prevention Research Institute of Texas, William H.Bisson was supported with funding from the NIH P30 ES000210, Yon Rojanasakul was supported with NIH grant R01-ES022968, Zhenbang Chen is supported by NIH grants (MD004038, CA163069 and MD007593), Zhiwei Hu is grateful for the grant support from an institutional start-up fund from The Ohio State University College of Medicine and The OSU James Comprehensive Cancer Center (OSUCCC) and a Seed Award from the OSUCCC Translational Therapeutics Program., Sans affiliation, Courcelles, Michel, Goodson, W, Lowe, L, Carpenter, D, Gilbertson, M, Ali, A, de Cerain Salsamendi, A, Lasfar, A, Carnero, A, Azqueta, A, Amedei, A, Charles, A, Collins, A, Ward, A, Salzberg, A, Colacci, A, Olsen, A, Berg, A, Barclay, B, Zhou, B, Blanco Aparicio, C, Baglole, C, Dong, C, Mondello, C, Hsu, C, Naus, C, Yedjou, C, Curran, C, Laird, D, Koch, D, Carlin, D, Felsher, D, Roy, D, Brown, D, Ratovitski, E, Ryan, E, Corsini, E, Rojas, E, Moon, E, Laconi, E, Marongiu, F, Al Mulla, F, Chiaradonna, F, Darroudi, F, Martin, F, Van Schooten, F, Goldberg, G, Wagemaker, G, Nangami, G, Calaf, G, Williams, G, Wolf, G, Koppen, G, Brunborg, G, Kim Lyerly, H, Krishnan, H, Hamid, H, Yasaei, H, Sone, H, Kondoh, H, Salem, H, Hsu, H, Park, H, Koturbash, I, Miousse, I, Ivana Scovassi, A, Klaunig, J, Vondráček, J, Raju, J, Roman, J, Wise, J, Whitfield, J, Woodrick, J, Christopher, J, Ochieng, J, Martinez Leal, J, Weisz, J, Kravchenko, J, Sun, J, Prudhomme, K, Narayanan, K, Cohen Solal, K, Moorwood, K, Gonzalez, L, Soucek, L, Jian, L, D'Abronzo, L, Lin, L, Li, L, Gulliver, L, Mccawley, L, Memeo, L, Vermeulen, L, Leyns, L, Zhang, L, Valverde, M, Khatami, M, Romano, M, Chapellier, M, Williams, M, Wade, M, Manjili, M, Lleonart, M, Xia, M, Gonzalez, M, Karamouzis, M, Kirsch Volders, M, Vaccari, M, Kuemmerle, N, Singh, N, Cruickshanks, N, Kleinstreuer, N, Van Larebeke, N, Ahmed, N, Ogunkua, O, Krishnakumar, P, Vadgama, P, Marignani, P, Ghosh, P, Ostrosky Wegman, P, Thompson, P, Dent, P, Heneberg, P, Darbre, P, Leung, P, Nangia Makker, P, Cheng, Q, Brooks Robey, R, Al Temaimi, R, Roy, R, Andrade Vieira, R, Sinha, R, Mehta, R, Vento, R, Di Fiore, R, Ponce Cusi, R, Dornetshuber Fleiss, R, Nahta, R, Castellino, R, Palorini, R, Hamid, R, Langie, S, Eltom, S, Brooks, S, Ryeom, S, Wise, S, Bay, S, Harris, S, Papagerakis, S, Romano, S, Pavanello, S, Eriksson, S, Forte, S, Casey, S, Luanpitpong, S, Lee, T, Otsuki, T, Chen, T, Massfelder, T, Sanderson, T, Guarnieri, T, Hultman, T, Dormoy, V, Odero Marah, V, Sabbisetti, V, Maguer Satta, V, Kimryn Rathmell, W, Engström, W, Decker, W, Bisson, W, Rojanasakul, Y, Luqmani, Y, Chen, Z, and Hu, Z
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Cancer Research ,Carcinogenesis ,[SDV]Life Sciences [q-bio] ,METHOXYCHLOR-INDUCED ALTERATIONS ,Review ,Pharmacology ,MESH: Carcinogens, Environmental ,Carcinogenic synergies ,Chemical mixtures ,Neoplasms ,MESH: Animals ,MESH: Neoplasms ,Carcinogenesi ,Risk assessment ,Cancer ,ACTIVATED PROTEIN-KINASES ,Medicine (all) ,Low dose ,1. No poverty ,Cumulative effects ,BREAST-CANCER CELLS ,General Medicine ,Environmental exposure ,MESH: Carcinogenesis ,BIO/10 - BIOCHIMICA ,EPITHELIAL-MESENCHYMAL TRANSITION ,3. Good health ,[SDV] Life Sciences [q-bio] ,Environmental Carcinogenesis ,ESTROGEN-RECEPTOR-ALPHA ,Human ,MESH: Environmental Exposure ,ENDOCRINE-DISRUPTING CHEMICALS ,TARGETING TISSUE FACTOR ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,Biology ,Prototypical chemical disruptors ,Exposure ,[SDV.CAN] Life Sciences [q-bio]/Cancer ,Environmental health ,medicine ,[SDV.EE.SANT] Life Sciences [q-bio]/Ecology, environment/Health ,Carcinogen ,Environmental carcinogenesis ,[SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health ,MESH: Humans ,Animal ,POLYBROMINATED DIPHENYL ETHERS ,Environmental Exposure ,medicine.disease ,MESH: Hazardous Substances ,Carcinogens, Environmental ,MIGRATION INHIBITORY FACTOR ,VASCULAR ENDOTHELIAL-CELLS ,Hazardous Substance ,Neoplasm - Abstract
Goodson, William H. et al., © The Author 2015. Lifestyle factors are responsible for a considerable portion of cancer incidence worldwide, but credible estimates from the World Health Organization and the International Agency for Research on Cancer (IARC) suggest that the fraction of cancers attributable to toxic environmental exposures is between 7% and 19%. To explore the hypothesis that low-dose exposures to mixtures of chemicals in the environment may be combining to contribute to environmental carcinogenesis, we reviewed 11 hallmark phenotypes of cancer, multiple priority target sites for disruption in each area and prototypical chemical disruptors for all targets, this included dose-response characterizations, evidence of low-dose effects and cross-hallmark effects for all targets and chemicals. In total, 85 examples of chemicals were reviewed for actions on key pathways/ mechanisms related to carcinogenesis. Only 15% (13/85) were found to have evidence of a dose-response threshold, whereas 59% (50/85) exerted low-dose effects. No dose-response information was found for the remaining 26% (22/85). Our analysis suggests that the cumulative effects of individual (non-carcinogenic) chemicals acting on different pathways, and a variety of related systems, organs, tissues and cells could plausibly conspire to produce carcinogenic synergies. Additional basic research on carcinogenesis and research focused on low-dose effects of chemical mixtures needs to be rigorously pursued before the merits of this hypothesis can be further advanced. However, the structure of the World Health Organization International Programme on Chemical Safety 'Mode of Action' framework should be revisited as it has inherent weaknesses that are not fully aligned with our current understanding of cancer biology., We gratefully acknowledge the support of the National Institute of Health-National Institute of Environmental Health Sciences (NIEHS) conference grant travel support (R13ES023276); Glenn Rice, Office of Research and Development, United States Environmental Protection Agency, Cincinnati, OH, USA also deserves thanks for his thoughtful feedback and inputs on the manuscript; William H.Goodson III was supported by the California Breast Cancer Research Program, Clarence Heller Foundation and California Pacific Medical Center Foundation; Abdul M.Ali would like to acknowledge the financial support of the University of Sultan Zainal Abidin, Malaysia; Ahmed Lasfar was supported by an award from the Rutgers Cancer Institute of New Jersey; Ann-Karin Olsen and Gunnar Brunborg were supported by the Research Council of Norway (RCN) through its Centres of Excellence funding scheme (223268/F50), Amancio Carnero’s lab was supported by grants from the Spanish Ministry of Economy and Competitivity, ISCIII (Fis: PI12/00137, RTICC: RD12/0036/0028) co-funded by FEDER from Regional Development European Funds (European Union), Consejeria de Ciencia e Innovacion (CTS-1848) and Consejeria de Salud of the Junta de Andalucia (PI-0306-2012); Matilde E. Lleonart was supported by a trienal project grant PI12/01104 and by project CP03/00101 for personal support. Amaya Azqueta would like to thank the Ministerio de Educacion y Ciencia (‘Juande la Cierva’ programme, 2009) of the Spanish Government for personal support; Amedeo Amedei was supported by the Italian Ministry of University and Research (2009FZZ4XM_002), and the University of Florence (ex60%2012); Andrew R.Collins was supported by the University of Oslo; Annamaria Colacci was supported by the Emilia-Romagna Region - Project ‘Supersite’ in Italy; Carolyn Baglole was supported by a salary award from the Fonds de recherche du Quebec-Sante (FRQ-S); Chiara Mondello’s laboratory is supported by Fondazione Cariplo in Milan, Italy (grant n. 2011-0370); Christian C.Naus holds a Canada Research Chair; Clement Yedjou was supported by a grant from the National Institutes of Health (NIH-NIMHD grant no. G12MD007581); Daniel C.Koch is supported by the Burroughs Wellcome Fund Postdoctoral Enrichment Award and the Tumor Biology Training grant: NIH T32CA09151; Dean W. Felsher would like to acknowledge the support of United States Department of Health and Human Services, NIH grants (R01 CA170378 PQ22, R01 CA184384, U54 CA149145, U54 CA151459, P50 CA114747 and R21 CA169964); Emilio Rojas would like to thank CONACyT support 152473, Ezio Laconi was supported by AIRC (Italian Association for Cancer Research, grant no. IG 14640) and by the Sardinian Regional Government (RAS); Eun-Yi Moon was supported by grants from the Public Problem-Solving Program (NRF-015M3C8A6A06014500) and Nuclear R&D Program (#2013M2B2A9A03051296 and 2010-0018545) through the National Research Foundation of Korea (NRF) and funded by the Ministry of Education, Science and Technology (MEST) in Korea; Fahd Al-Mulla was supported by the Kuwait Foundation for the Advancement of Sciences (2011-1302-06); Ferdinando Chiaradonna is supported by SysBioNet, a grant for the Italian Roadmap of European Strategy Forum on Research Infrastructures (ESFRI) and by AIRC (Associazione Italiana Ricerca sul Cancro; IG 15364); Francis L.Martin acknowledges funding from Rosemere Cancer Foundation; he also thanks Lancashire Teaching Hospitals NHS trust and the patients who have facilitated the studies he has undertaken over the course of the last 10 years; Gary S.Goldberg would like to acknowledge the support of the New Jersey Health Foundation; Gloria M.Calaf was supported by Fondo Nacional de Ciencia y Tecnología (FONDECYT), Ministerio de Educación de Chile (MINEDUC), Universidad de Tarapacá (UTA); Gudrun Koppen was supported by the Flemish Institute for Technological Research (VITO), Belgium; Hemad Yasaei was supported from a triennial project grant (Strategic Award) from the National Centre for the Replacement, Refinement and Reduction (NC3Rs) of animals in research (NC.K500045.1 and G0800697); Hiroshi Kondoh was supported in part by grants from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, Japan Science and Technology Agency and by JST, CREST; Hsue-Yin Hsu was supported by the Ministry of Science and Technology of Taiwan (NSC93-2314-B-320-006 and NSC94-2314-B-320-002); Hyun Ho Park was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) of the Ministry of Education, Science and Technology (2012R1A2A2A01010870) and a grant from the Korea Healthcare Technology R&D project, Ministry of Health and Welfare, Republic of Korea (HI13C1449); Igor Koturbash is supported by the UAMS/NIH Clinical and Translational Science Award (UL1TR000039 and KL2TR000063) and the Arkansas Biosciences Institute, the major research component of the Arkansas Tobacco Settlement Proceeds Act of 2000; Jan Vondráček acknowledges funding from the Czech Science Foundation (13-07711S); Jesse Roman thanks the NIH for their support (CA116812), John Pierce Wise Sr. and Sandra S.Wise were supported by National Institute of Environmental Health Sciences (ES016893 to J.P.W.) and the Maine Center for Toxicology and Environmental Health; Jonathan Whitfield acknowledges support from the FERO Foundation in Barcelona, Spain; Joseph Christopher is funded by Cancer Research UK and the International Journal of Experimental Pathology; Julia Kravchenko is supported by a philanthropic donation by Fred and Alice Stanback; Jun Sun is supported by a Swim Across America Cancer Research Award; Karine A.Cohen-Solal is supported by a research scholar grant from the American Cancer Society (116683-RSG-09-087-01-TBE); Laetitia Gonzalez received a postdoctoral fellowship from the Fund for Scientific Research–Flanders (FWO-Vlaanderen) and support by an InterUniversity Attraction Pole grant (IAP-P7-07); Laura Soucek is supported by grant #CP10/00656 from the Miguel Servet Research Contract Program and acknowledges support from the FERO Foundation in Barcelona, Spain; Liang-Tzung Lin was supported by funding from the Taipei Medical University (TMU101-AE3-Y19); Linda Gulliver is supported by a Genesis Oncology Trust (NZ) Professional Development Grant, and the Faculty of Medicine, University of Otago, Dunedin, New Zealand; Louis Vermeulen is supported by a Fellowship of the Dutch Cancer Society (KWF, UVA2011-4969) and a grant from the AICR (14–1164); Mahara Valverde would like to thank CONACyT support 153781; Masoud H. Manjili was supported by the office of the Assistant Secretary of Defense for Health Affairs (USA) through the Breast Cancer Research Program under Award No. W81XWH-14-1-0087 Neetu Singh was supported by grant #SR/FT/LS-063/2008 from the Department of Science and Technology, Government of India; Nicole Kleinstreuer is supported by NIEHS contracts (N01-ES 35504 and HHSN27320140003C); P.K. Krishnakumar is supported by the Funding (No. T.K. 11-0629) of King Abdulaziz City for Science and Technology, Riyadh, Saudi Arabia; Paola A.Marignani is supported by the Dalhousie Medical Research Foundation, The Beatrice Hunter Cancer Institute and CIHR and the Nova Scotia Lung Association; Paul Dent is the holder of the Universal Inc.Chair in Signal Transduction Research and is supported with funds from PHS grants from the NIH (R01-CA141704, R01-CA150214, R01-DK52825 and R01-CA61774); Petr Heneberg was supported by the Charles University in Prague projects UNCE 204015 and PRVOUK P31/2012, and by the Czech Science Foundation projects P301/12/1686 and 15-03834Y; Po Sing Leung was supported by the Health and Medical Research Fund of Food and Health Bureau, Hong Kong Special Administrative Region, Ref. No: 10110021; Qiang Cheng was supported in part by grant NSF IIS-1218712; R. Brooks Robey is supported by the United States Department of Veterans Affairs; Rabindra Roy was supported by United States Public Health Service Grants (RO1 CA92306, RO1 CA92306-S1 and RO1 CA113447); Rafaela Andrade-Vieira is supported by the Beatrice Hunter Cancer Research Institute and the Nova Scotia Health Research Foundation, Renza Vento was partially funded by European Regional Development Fund, European Territorial Cooperation 2007–13 (CCI 2007 CB 163 PO 037, OP Italia-Malta 2007–13) and grants from the Italian Ministry of Education, University and Research (MIUR) ex-60%, 2007; Riccardo Di Fiore was a recipient of fellowship granted by European Regional Development Fund, European Territorial Cooperation 2007–2013 (CCI 2007 CB 163 PO 037, OP Italia-Malta 2007–2013); Rita Dornetshuber-Fleiss was supported by the Austrian Science Fund (FWF, project number T 451-B18) and the Johanna Mahlke, geb.-Obermann-Stiftung; Roberta Palorini is supported by a SysBioNet fellowship; Roslida Abd Hamid is supported by the Ministry of Education, Malaysia-Exploratory Research Grant Scheme-Project no: ERGS/1-2013/5527165; Sabine A.S.Langie is the beneficiary of a postdoctoral grant from the AXA Research Fund and the Cefic-LRI Innovative Science Award 2013; Sakina Eltom is supported by NIH grant SC1CA153326; Samira A.Brooks was supported by National Research Service Award (T32 ES007126) from the National Institute of Environmental Health Sciences and the HHMI Translational Medicine Fellowship; Sandra Ryeom was supported by The Garrett B. Smith Foundation and the TedDriven Foundation; Thierry Massfelder was supported by the Institut National de la Santé et de la Recherche Médicale INSERM and Université de Strasbourg; Thomas Sanderson is supported by the Canadian Institutes of Health Research (CIHR; MOP-115019), the Natural Sciences and Engineering Council of Canada (NSERC; 313313) and the California Breast Cancer Research Program (CBCRP; 17UB-8703); Tiziana Guarnieri is supported by a grant from Fundamental Oriented Research (RFO) to the Alma Mater Studiorum University of Bologna, Bologna, Italy and thanks the Fondazione Cassa di Risparmio di Bologna and the Fondazione Banca del Monte di Bologna e Ravenna for supporting the Center for Applied Biomedical Research, S.Orsola-Malpighi University Hospital, Bologna, Italy; W.Kimryn Rathmell is supported by the V Foundation for Cancer Research and the American Cancer Society; William K.Decker was supported in part by grant RP110545 from the Cancer Prevention Research Institute of Texas; William H.Bisson was supported with funding from the NIH P30 ES000210; Yon Rojanasakul was supported with NIH grant R01-ES022968; Zhenbang Chen is supported by NIH grants (MD004038, CA163069 and MD007593); Zhiwei Hu is grateful for the grant support from an institutional start-up fund from The Ohio State University College of Medicine and The OSU James Comprehensive Cancer Center (OSUCCC) and a Seed Award from the OSUCCC Translational Therapeutics Program.
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188. A novel molecular imprinted polymer layer electrode for enhanced sensitivity electrochemical determination of the antidepressant fluoxetine.
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Feroz, Momina, Lopes, Ilanna Campelo, Rehman, Habib ur, Ata, Sadia, and Vadgama, Pankaj
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POLYMER electrodes , *IMPRINTED polymers , *CARBON electrodes , *SEROTONIN antagonists , *ANTIDEPRESSANTS , *BULIMIA - Abstract
Fluoxetine is a selective serotonin re-uptake inhibitor (SSRI) drug, widely used to treat depression, nervous anorexia, autism and bulimia nervosa. The direct electrochemical behaviour of fluoxetine at a glassy carbon electrode (GCE) was studied in simple aqueous solution using voltammetric techniques. Subsequently, a novel molecular imprinted polymer (MIP) modified GCE was fabricated using itaconic acid and N , N -methylene-bis acrylamide as functional monomer and crosslinking agent, respectively, to produce a sensitive, selective fluoxetine sensor. Precipitation polymerization enabled drop coating of reproducible MIP layers on a GCE as a renewable affinity surface for fluoxetine detection. Initial baseline studies undertaken using CV and SWV allowed for rapid optimization of scan conditions and confirming the irreversible nature of the FXT reaction at a bare GCE but DPV finally allowed for the further and necessary high level sensitivity needed for assays. The binding efficiency and selectivity of the MIP affinity sites for fluoxetine were demonstrated using DPV and responses compared with a non-imprinted polymer layer (NIP). Conditions for MIP formation, loading and incubation were optimized to improve the analytical performance. Under optimized conditions, a linear fluoxetine calibration from 4.99 × 10−7 to 3.38 × 10−5 mol L−1 was obtained, with detection and quantification limits of 3.33 × 10−7 mol L−1 (115.01 μg L−1) and 1.11 × 10−6 mol L−1 (383.38 μg L−1), respectively. The sensor was employed to analyse fluoxetine extracted from blood serum. Unlabelled Image • The electrochemical behaviour of Fluoxetine was studied at a GCE by CV, DPV and SWV. • A MIP was synthesized by rapid and convenient photopolymerization and characterized by FTIR, TGA, SEM and BET. • Assay conditions were optimized and Fluoxetine measurement made on solvent extracted blood serum. • The MIP formulation enabled generation of reproducible electrode coatings. [ABSTRACT FROM AUTHOR]
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- 2020
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189. Identification and Quantitative Determination of Lactate Using Optical Spectroscopy—Towards a Noninvasive Tool for Early Recognition of Sepsis †.
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Budidha, Karthik, Mamouei, Mohammad, Baishya, Nystha, Qassem, Meha, Vadgama, Pankaj, and Kyriacou, Panayiotis A.
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OPTICAL spectroscopy , *BLOOD lactate , *REGRESSION analysis , *NEAR infrared spectroscopy , *ABSORPTION spectra , *INTRA-aortic balloon counterpulsation , *SEPSIS - Abstract
Uninterrupted monitoring of serum lactate levels is a prerequisite in the critical care of patients prone to sepsis, cardiogenic shock, cardiac arrest, or severe lung disease. Yet there exists no device to continuously measure blood lactate in clinical practice. Optical spectroscopy together with multivariate analysis is proposed as a viable noninvasive tool for estimation of lactate in blood. As an initial step towards this goal, we inspected the plausibility of predicting the concentration of sodium lactate (NaLac) from the UV/visible, near-infrared (NIR), and mid-infrared (MIR) spectra of 37 isotonic phosphate-buffered saline (PBS) samples containing NaLac ranging from 0 to 20 mmol/L. UV/visible (300–800 nm) and NIR (800–2600 nm) spectra of PBS samples were collected using the PerkinElmer Lambda 1050 dual-beam spectrophotometer, while MIR (4000–500 cm−1) spectra were collected using the Spectrum two FTIR spectrometer. Absorption bands in the spectra of all three regions were identified and functional groups were assigned. The concentration of lactate in samples was predicted using the Partial Least-Squares (PLS) regression analysis and leave-one-out cross-validation. The regression analysis showed a correlation coefficient (R2) of 0.926, 0.977, and 0.992 for UV/visible, NIR, and MIR spectra, respectively, between the predicted and reference samples. The RMSECV of UV/visible, NIR, and MIR spectra was 1.59, 0.89, and 0.49 mmol/L, respectively. The results indicate that optical spectroscopy together with multivariate models can achieve a superior technique in assessing lactate concentrations. [ABSTRACT FROM AUTHOR]
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190. Investigations into the Effects of pH on Quantitative Measurements of Lactate in Biological Media Using ATR-FTIR Spectroscopy.
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Baishya, Nystha, Mamouei, Mohammad, Budidha, Karthik, Qassem, Meha, Vadgama, Pankaj, Kyriacou, Panayiotis A., and McPhee, Derek J.
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PH effect , *LACTIC acid , *SEPTIC shock , *CELL respiration , *BIOMARKERS - Abstract
Quantification of lactate/lactic acid in critical care environments is essential as lactate serves as an important biochemical marker for the adequacy of the haemodynamic circulation in shock and of cell respiration at the onset of sepsis/septic shock. Hence, in this study, ATR-FTIR was explored as a potential tool for lactate measurement, as the current techniques depend on sample preparation and fails to provide rapid response. Moreover, the effects of pH on PBS samples (7.4, 7, 6.5 and 6) and change in solution conditions (PBS to whole blood) on spectral features were also investigated. A total 189 spectra from five sets of lactate containing media were obtained. Results suggests that lactate could be measured with more than 90% accuracy in the wavenumber range of 1500–600 cm − 1 . The findings of this study further suggest that there exist no effects of change in pH or media, when estimating lactate concentration changes in this range of the Mid-IR spectral region. [ABSTRACT FROM AUTHOR]
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- 2020
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191. Nanopatterned tubular collagen scaffolds for vascular tissue engineering
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Zorlutuna, Pinar, Hasırcı, Vasıf Nejat, Vadgama, Pankaj, and Biyoteknoloji Anabilim Dalı
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Tissue engineering ,Biyoteknoloji ,Biotechnology - Abstract
Gelişmiş ülkelerde başta gelen ölüm sebeplerinden biri, küçük ve orta çaplı damarları etkileyen kalp damar hastalıklarıdır. Bu gibi durumlarda genellikle sınırlı miktarda olan otolog damarlar kullanılmaktadır. Doku mühendisliği ile üretilmiş bir yapay damar bu soruna nihai çözüm olabilir. Hücrelerin, hücre taşıyıcıları üzerinde, doğal dokuda oldu gibi organize bir şekilde büyütülmesi geliştirilmiş mekanik özelliklere sahip yapay dokuların elde edilmesi için araştırılmaktadır. Bu çalışmada 650 nm, 500 nm ve 332.5 nm eninde kanallara sahip kolajen hücre taşıyıcılarına insan damar düz kas ve insan endotel hücreleri önce ayrı ayrı ekilmiş sonra da tübüler formdaki taşıyıcılarda ko-kültüre edilmişlerdir. Film şeklindeki taşıyıcılar endotel hücreleri ile ekildiklerinde nanodesenlerin hücreleri yönlendirmediği, yüzeye ilk tutunmalarını ve yüzeyde çoğalmalarını etkilemedikleri, buna karşın sıvı akışı altında hücrelerin yüzeyde kalmalarını belirgin ölçüde arttırdıkları gözlenmiştir. Desensiz yüzeylerde sıvı akışı uygulandıktan sonraki hücre miktarı ilk ekilenin % 35 ± 10'i iken 332.5 nm desenli yüzeyde % 75 ± 4 ve 650 nm desenli yüzeyde % 91 ± 5 olarak bulunmuştur. Düz kas hücreleri ile ekildiklerinde 332.5 nm olan en küçük boyutlu desenin bile hücre yönlenmesini sağladığı ve bu yönlenmenin mekanik özellikleri arttırdığı görülmüştür. Nanodesenlerin varlığı mutlak kopma mukavemetini (UTS) sıfırıncı gündeki değer olan 0.55 ± 0.11'den 75. günde 1.63 ± 0.46 MPa'a kadar çıkarmıştır ki bu değer doğal damarların UTS değer aralığı içindedir. 4 MPa civarında bulunan Elastik Modül de bu aralık içerisindedir. Bu filmler son kademede sarılarak tüp şekline çevrildikleri için hücrelerin beslenmesinde rol oynayacak olan moleküler difüzyon, oksijen and 4-asetaminofenol molekülleri için incelenmiş ve hücresiz formda sırasıyla 1.86 ± 0.39 x 10-7 cm2.s-1 ve 5.41 ± 2.14 x 10-7 cm2.s-1 olarak bulunan bu değerler hücre ekildiğinde 4 katına çıkmıştır ki, bu değerler doğal dokuların değer aralığındadır. Tüp şeklindeki taşıyıcı (iki tarafında da nanodesen bulunan kolajen tüp) üzerinde her iki tip hücre ko-kültüre edildiklerinde düz kas hücrelerinin tüpün dış tarafında büyüdüğü ve yönlendikleri, ve endotellerin tüpün iç yüzeyinde tek katman halinde büyüdükleri gözlemlenmiştir.Sonuç olarak bu çalışma hücre yönlendirmesinin doku mühendisliği yöntemleri ile elde edilmiş damarların mekanik özelliklerinin arttırılmasında kullanılabileceğini ve damar doku mühendisliğinin en önemli iki sorunu olan yetersiz mekanik özellikler ve sıvı akışı koşullarında tek katman halinde bir endotel tabakası elde edilmesinin başarılmasında kullanılabilecegini göstermiştir. One of the major causes of death in developed countries is cardiovascular disease that affects small and medium sized blood vessels. In most cases autologous grafts have to be used which have limited availability. A functional tissue engineered vessel can be the ultimate solution for vascular reconstruction. Tissue engineered constructs with cells growing in an organized manner have been shown to have improved mechanical properties. In the present study collagen scaffolds with 650 nm, 500 nm and 332.5 nm wide channels and ridges were seeded with human vascular smooth muscle cells (VSMC) and human endothelial cells seperately and then co-cultured on tubular scaffolds. When the films were seeded with endothelial cells it was observed that nanopatterns do not affect cell proliferation or initial cell alignment; however, they significantly influenced cell retention under shear (fluid flow). While 35 ± 10 % of the cells were retained on unpatterned films, 75 ± 4 % was retained on 332.5 nm patterned films and even higher, 91 ± 5 % was retained on 650 nm patterned films. It was shown that nanopatterns as small as 332.5 nm could align the vascular smooth muscle cells (VSMC) and that alignment significantly improved mechanical properties. Presence of nanopatterns increased the ultimate tensile strength (UTS) from 0.55 ± 0.11 on Day 0 to as much as 1.63 ± 0.46 MPa on Day 75, a value within the range of natural arteries and veins. Similarly, Young?s Modulus values were ca. 4 MPa, again in the range of the natural vessels. Since the films would be ultimately rolled into tubes of collagen, nutrient transfer through the films is quite crucial. Diffusion coefficient for 4-acetaminophenol and oxygen through the collagen films were found to be 1.86 ± 0.39 x 10-7 cm2.s-1 and 5.41 ± 2.14 x 10-7 cm2.s-1, repectively in the unseeded form, and increased by 4 fold after cell seeding, which is comparable to that in natural tissues. When both cell types were co-cultured on the nanopatterned tubes (a both-side nanopatterned collagen tube), it was shown that on the outside of the tube VSMCs proliferated in an oriented manner and on the inside endothelial cells proliferated as a monolayer.Therefore, this study showed that cell guidance enhances the mechanical properties of engineered vessels, and help overcome the two most important challenges in vascular tissue engineering; the need for adequate mechanical properties and continuous lining of endothelial cells even under physiological shear stress. 147
- Published
- 2009
192. A naringin-derived bioink enhances the shape fidelity of 3D bioprinting and efficiency of cartilage defect repair.
- Author
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Huang Y, Meng X, Zhou Z, Zhu W, Chen X, He Y, He N, Han X, Zhou D, Duan X, Vadgama PM, and Liu H
- Subjects
- Animals, Cartilage, Flavanones, Gelatin, Printing, Three-Dimensional, Rabbits, Superoxide Dismutase-1, Tissue Engineering methods, Bioprinting methods
- Abstract
3D bioprinting is a major area of interest in health sciences for customized manufacturing, but lacks specific bioinks to enhance the shape fidelity of 3D bioprinting and efficiency of tissue repair for particular clinical purposes. A naringin derived bioink, which contains 1.5 mM methylacryloyl naringin and 0.15 mM methylacryloyl gelatin, improves the fidelity of 3D bioprinting due to 405 nm light absorption of methylacryloyl naringin. The naringin derived bioink promotes the growth of chondrocytes due to preserving bioactivities of naringin and functions as a medical ingredient from which it has been described as a medical bioink in this study. It facilitates cartilage regeneration by upregulating the transcription of chondrogenesis-related genes like SOX9 and genes against oxidative stress like SOD1 and SOD2 and maintains chondrocytes active resulting from the significantly enhanced COL II / COL I ratio. According to a rabbit cartilage defect model, the proposed naringin derived medical bioink significantly improves the efficiency and quality of cartilage defect repair, suggesting that the bioink is suitable for cartilage defect repair applications and a feasible strategy is provided for the formulation of medical bioinks for specific clinical purposes.
- Published
- 2022
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193. Monitoring with In Vivo Electrochemical Sensors: Navigating the Complexities of Blood and Tissue Reactivity.
- Author
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Vadgama P
- Subjects
- Glucose analysis, Humans, Lactic Acid analysis, Prostheses and Implants, Reproducibility of Results, Biosensing Techniques, Electrochemical Techniques instrumentation, Monitoring, Physiologic
- Abstract
The disruptive action of an acute or critical illness is frequently manifest through rapid biochemical changes that may require continuous monitoring. Within these changes, resides trend information of predictive value, including responsiveness to therapy. In contrast to physical variables, biochemical parameters monitored on a continuous basis are a largely untapped resource because of the lack of clinically usable monitoring systems. This is despite the huge testing repertoire opening up in recent years in relation to discrete biochemical measurements. Electrochemical sensors offer one of the few routes to obtaining continuous readout and, moreover, as implantable devices information referable to specific tissue locations. This review focuses on new biological insights that have been secured through in vivo electrochemical sensors. In addition, the challenges of operating in a reactive, biological, sample matrix are highlighted. Specific attention is given to the choreographed host rejection response, as evidenced in blood and tissue, and how this limits both sensor life time and reliability of operation. Examples will be based around ion, O
2 , glucose, and lactate sensors, because of the fundamental importance of this group to acute health care.- Published
- 2020
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194. Assessing the carcinogenic potential of low-dose exposures to chemical mixtures in the environment: the challenge ahead.
- Author
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Goodson WH 3rd, Lowe L, Carpenter DO, Gilbertson M, Manaf Ali A, Lopez de Cerain Salsamendi A, Lasfar A, Carnero A, Azqueta A, Amedei A, Charles AK, Collins AR, Ward A, Salzberg AC, Colacci A, Olsen AK, Berg A, Barclay BJ, Zhou BP, Blanco-Aparicio C, Baglole CJ, Dong C, Mondello C, Hsu CW, Naus CC, Yedjou C, Curran CS, Laird DW, Koch DC, Carlin DJ, Felsher DW, Roy D, Brown DG, Ratovitski E, Ryan EP, Corsini E, Rojas E, Moon EY, Laconi E, Marongiu F, Al-Mulla F, Chiaradonna F, Darroudi F, Martin FL, Van Schooten FJ, Goldberg GS, Wagemaker G, Nangami GN, Calaf GM, Williams G, Wolf GT, Koppen G, Brunborg G, Lyerly HK, Krishnan H, Ab Hamid H, Yasaei H, Sone H, Kondoh H, Salem HK, Hsu HY, Park HH, Koturbash I, Miousse IR, Scovassi AI, Klaunig JE, Vondráček J, Raju J, Roman J, Wise JP Sr, Whitfield JR, Woodrick J, Christopher JA, Ochieng J, Martinez-Leal JF, Weisz J, Kravchenko J, Sun J, Prudhomme KR, Narayanan KB, Cohen-Solal KA, Moorwood K, Gonzalez L, Soucek L, Jian L, D'Abronzo LS, Lin LT, Li L, Gulliver L, McCawley LJ, Memeo L, Vermeulen L, Leyns L, Zhang L, Valverde M, Khatami M, Romano MF, Chapellier M, Williams MA, Wade M, Manjili MH, Lleonart ME, Xia M, Gonzalez MJ, Karamouzis MV, Kirsch-Volders M, Vaccari M, Kuemmerle NB, Singh N, Cruickshanks N, Kleinstreuer N, van Larebeke N, Ahmed N, Ogunkua O, Krishnakumar PK, Vadgama P, Marignani PA, Ghosh PM, Ostrosky-Wegman P, Thompson PA, Dent P, Heneberg P, Darbre P, Sing Leung P, Nangia-Makker P, Cheng QS, Robey RB, Al-Temaimi R, Roy R, Andrade-Vieira R, Sinha RK, Mehta R, Vento R, Di Fiore R, Ponce-Cusi R, Dornetshuber-Fleiss R, Nahta R, Castellino RC, Palorini R, Abd Hamid R, Langie SA, Eltom SE, Brooks SA, Ryeom S, Wise SS, Bay SN, Harris SA, Papagerakis S, Romano S, Pavanello S, Eriksson S, Forte S, Casey SC, Luanpitpong S, Lee TJ, Otsuki T, Chen T, Massfelder T, Sanderson T, Guarnieri T, Hultman T, Dormoy V, Odero-Marah V, Sabbisetti V, Maguer-Satta V, Rathmell WK, Engström W, Decker WK, Bisson WH, Rojanasakul Y, Luqmani Y, Chen Z, and Hu Z
- Subjects
- Animals, Humans, Carcinogenesis chemically induced, Carcinogens, Environmental adverse effects, Environmental Exposure adverse effects, Hazardous Substances adverse effects, Neoplasms chemically induced, Neoplasms etiology
- Abstract
Lifestyle factors are responsible for a considerable portion of cancer incidence worldwide, but credible estimates from the World Health Organization and the International Agency for Research on Cancer (IARC) suggest that the fraction of cancers attributable to toxic environmental exposures is between 7% and 19%. To explore the hypothesis that low-dose exposures to mixtures of chemicals in the environment may be combining to contribute to environmental carcinogenesis, we reviewed 11 hallmark phenotypes of cancer, multiple priority target sites for disruption in each area and prototypical chemical disruptors for all targets, this included dose-response characterizations, evidence of low-dose effects and cross-hallmark effects for all targets and chemicals. In total, 85 examples of chemicals were reviewed for actions on key pathways/mechanisms related to carcinogenesis. Only 15% (13/85) were found to have evidence of a dose-response threshold, whereas 59% (50/85) exerted low-dose effects. No dose-response information was found for the remaining 26% (22/85). Our analysis suggests that the cumulative effects of individual (non-carcinogenic) chemicals acting on different pathways, and a variety of related systems, organs, tissues and cells could plausibly conspire to produce carcinogenic synergies. Additional basic research on carcinogenesis and research focused on low-dose effects of chemical mixtures needs to be rigorously pursued before the merits of this hypothesis can be further advanced. However, the structure of the World Health Organization International Programme on Chemical Safety 'Mode of Action' framework should be revisited as it has inherent weaknesses that are not fully aligned with our current understanding of cancer biology., (© The Author 2015. Published by Oxford University Press.)
- Published
- 2015
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195. Clinical context and fundamental advances: the need to build linkages.
- Author
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Vadgama P
- Subjects
- Chemistry Techniques, Analytical trends, Diagnosis, Humans, Chemistry Techniques, Analytical methods, Diagnostic Techniques and Procedures
- Published
- 2011
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196. Modulation of cell growth on exposure to silkworm and spider silk fibers.
- Author
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Hakimi O, Gheysens T, Vollrath F, Grahn MF, Knight DP, and Vadgama P
- Subjects
- Animals, Biocompatible Materials chemistry, Biocompatible Materials toxicity, Blood Proteins metabolism, Cattle, Cell Culture Techniques, Cell Line, Cell Proliferation drug effects, Culture Media, Conditioned chemistry, Endothelial Cells cytology, Endothelial Cells metabolism, Humans, Materials Testing, Sericins chemistry, Sericins pharmacology, Sericins toxicity, Silk chemistry, Silk toxicity, Tissue Engineering instrumentation, Tissue Engineering methods, Biocompatible Materials pharmacology, Bombyx chemistry, Endothelial Cells drug effects, Silk pharmacology, Spiders chemistry
- Abstract
Recent years have seen an increased interest in the use of natural and modified silks for tissue engineering. Despite longstanding concerns regarding the biocompatibility of silk sutures, only a few studies have been carried out to investigate the biocompatibility of natural silk fibers. Here, we report an in vitro assessment of the effect of nonmodified, degummed silks on cells. We describe the effects of degummed silk fibers as well as extracted sericin on cell metabolism and proliferation. Endothelial cells directly exposed to native degummed Bombyx mori and Antheraea pernyi silks showed lower rates of proliferation and metabolism than nonexposed cells. A similar but milder effect was observed for cells in direct contact with Nephila edulis egg sack fibers. Sericin and silk-conditioned medium had no negative effect on cell proliferation except in medium supplemented with 5% bovine serum prior to conditioning with A. pernyi silk. The toxicity of A. pernyi was negligible after thorough enzymatic treatment of the fibers with trypsin. It is, therefore, proposed that A. pernyi silk contain one or more cytotoxic components, which need to be removed prior to medical use., ((c) 2009 Wiley Periodicals, Inc.)
- Published
- 2010
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197. Synthesis and characterisation of enhanced barrier polyurethane for encapsulation of implantable medical devices.
- Author
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Roohpour N, Wasikiewicz JM, Paul D, Vadgama P, and Rehman IU
- Subjects
- Calorimetry, Differential Scanning methods, Dimethylpolysiloxanes chemistry, Elasticity, Hydrophobic and Hydrophilic Interactions, Light, Magnetic Resonance Spectroscopy, Materials Testing, Polymers chemistry, Scattering, Radiation, Spectroscopy, Fourier Transform Infrared, Spectrum Analysis, Raman methods, Stress, Mechanical, Tensile Strength, Water chemistry, Polyurethanes chemical synthesis, Polyurethanes chemistry, Prostheses and Implants
- Abstract
Polymeric membranes have been used as interfaces between implantable devices and biological tissues to operate as a protective barrier from water exchanging and to enhance biocompatibility. Polyurethanes have been used as biocompatible membranes for decades. In this study, copolymers of polyether urethane (PEU) with polydimethylsiloxane (PDMS) were synthesised with the goal of creating materials with low water permeability and high elasticity. PDMS was incorporated into polymer backbone as a part of the soft segment during polyurethane synthesis and physical properties as well as water permeability of resulting copolymer were studied in regard to PDMS content. Increase in PDMS content led to increase of microphase separation of the copolymer and corresponding increase in elastic modulus. Surface energy of the polymer was decreased by incorporating PDMS compared to unmodified PEU. PDMS in copolymer formed a hydrophobic surface which caused reduction in water permeability and water uptake of the membranes. Thus, PDMS containing polyurethane with its potent water resistant properties demonstrated a great promise for use as an implantable encapsulation material.
- Published
- 2009
- Full Text
- View/download PDF
198. Assessment of tissue scaffold degradation using electrochemical techniques.
- Author
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Willows A, Fan Q, Ismail F, Vaz CM, Tomlins PE, Mikhalovska LI, Mikhalovsky SV, James SL, Vadgama P, and Wasikiewicz J
- Subjects
- Buffers, Collagen metabolism, Collagenases metabolism, Diffusion drug effects, Electrodes, Electrolytes, Ferrocyanides pharmacology, Glycosaminoglycans metabolism, Hydrogen-Ion Concentration, Membranes, Artificial, Microscopy, Electron, Scanning, Oxidation-Reduction drug effects, Permeability drug effects, Porosity drug effects, Solutions, Time Factors, Electrochemistry methods, Tissue Scaffolds
- Abstract
Degradation of a commercially available collagen-glycosaminoglycan dermal equivalent matrix was studied using electrochemical techniques. Degradation was accelerated by exposure to gamma radiation followed by storage at elevated temperatures or exposure to enzymes. The time-dependent diffusion of a small, electrochemically active, molecular probe, potassium ferrocyanide, through the matrix was monitored via changes in the oxidation peak currents of cyclic voltammograms. These measurements were made using a two-compartment diffusion chamber with the sample positioned well away from the working electrodes and a single-compartment electrode cell where the matrix was in direct contact with the working electrode. The relative merits of these two approaches are considered. Regardless of the approach chosen, amperometry appears well suited to monitoring progressive diffusivity changes through mechanically weak porous structures subject to different solution environments.
- Published
- 2008
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- View/download PDF
199. Simple expressions for diffusion coefficient determination of adsorption within spherical and cylindrical absorbents using direct simulation method.
- Author
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Rong Z and Vadgama P
- Subjects
- Kinetics, Porosity, Absorption, Models, Chemical
- Abstract
Various analytical expressions for solute adsorption kinetics within porous absorbents of defined geometry (planar sheet, cylinder, and sphere) are available in the literature. However, these expressions are limited for practical numerical evaluation because they are based on infinite series. An investigation of these expressions has been carried out and then accurate but simple expressions derived that enable rapid determination of effective diffusion coefficients for adsorption within geometrically categorical absorbents. These involve directly fitting calculated kinetic adsorption curves to experimental ones. A simple one point method is also proposed to estimate the effective diffusion coefficient for an adsorption process within these simple geometrical absorbents as an initial value for a best fit.
- Published
- 2006
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- View/download PDF
200. A lightweight measuring device for the continuous in vivo monitoring of glucose by means of ultraslow microdialysis in combination with a miniaturised flow-through biosensor.
- Author
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Rhemrev-Boom RM, Tiessen RG, Jonker AA, Venema K, Vadgama P, and Korf J
- Subjects
- Biosensing Techniques methods, Biosensing Techniques standards, Blood Glucose Self-Monitoring methods, Blood Glucose Self-Monitoring standards, Calibration, Diabetes Mellitus blood, Dialysis, Female, Glucose Oxidase metabolism, Glucose Tolerance Test, Humans, Hydrogen Peroxide analysis, Miniaturization, Reproducibility of Results, Sensitivity and Specificity, Time Factors, Biosensing Techniques instrumentation, Blood Glucose Self-Monitoring instrumentation
- Abstract
Background: Tight regulation of blood glucose levels from patients suffering from diabetes mellitus can significantly reduce the complications associated with this disease. For this reason, elaborate research efforts have been devoted to the development of a glucose sensor for the continuous in vivo monitoring of glucose. Although the use of microdialysis as a sampling interface between the body and the biosensor is widely accepted, a major drawback of conventional microdialysis is the limited in vivo recovery. Here, ultraslow microdialysis is proposed in order to obtain (near) quantitative in vivo recoveries. To avoid, however, unacceptable long delay times, the need for a small and low dead volume measuring device was recognised., Methods: A portable lightweight measuring device for continuous in vivo monitoring of glucose in subcutaneous tissue is presented. The measuring device consists of a miniaturised flow-through biosensor, connected to a microdialysis probe and a semi-vacuum pump. The biosensor is based on the amperometric detection of hydrogen peroxide after conversion of glucose by immobilised glucose oxidase. A portable potentiostat equipped with data logging is used for detection and registration., Results: The device was validated for its accuracy, precision, linearity, sensitivity, selectivity and stability during ex vivo and in vivo experiments. The linearity was found to be up to 30 mmol/l with a limit of detection of 0.05 mmol/l. The precision, depending on the biosensor tested was found to be 2-4%. No contribution to the signal could be observed from several tested electroactive species. The accuracy was found to be well in accordance with the criteria set for methods of Self Monitoring of Blood Glucose for patients with diabetes mellitus. The biosensors could be used for up to 3 days in the continuous mode. In vivo monitoring of glucose in dialysate of subcutaneous sampled tissue during glucose tolerance tests in healthy volunteers demonstrated the potential of this measuring device., Conclusions: A portable lightweight measuring device is presented which can measure continuously glucose in vivo without excessive calibration steps. The performance characteristics determined justify the application of this measuring device for the in vivo monitoring of glucose in subcutaneous sampled interstitium of diabetic patients.
- Published
- 2002
- Full Text
- View/download PDF
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