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Assessment of tissue scaffold degradation using electrochemical techniques.
- Source :
-
Acta biomaterialia [Acta Biomater] 2008 May; Vol. 4 (3), pp. 686-96. Date of Electronic Publication: 2007 Oct 30. - Publication Year :
- 2008
-
Abstract
- Degradation of a commercially available collagen-glycosaminoglycan dermal equivalent matrix was studied using electrochemical techniques. Degradation was accelerated by exposure to gamma radiation followed by storage at elevated temperatures or exposure to enzymes. The time-dependent diffusion of a small, electrochemically active, molecular probe, potassium ferrocyanide, through the matrix was monitored via changes in the oxidation peak currents of cyclic voltammograms. These measurements were made using a two-compartment diffusion chamber with the sample positioned well away from the working electrodes and a single-compartment electrode cell where the matrix was in direct contact with the working electrode. The relative merits of these two approaches are considered. Regardless of the approach chosen, amperometry appears well suited to monitoring progressive diffusivity changes through mechanically weak porous structures subject to different solution environments.
- Subjects :
- Buffers
Collagen metabolism
Collagenases metabolism
Diffusion drug effects
Electrodes
Electrolytes
Ferrocyanides pharmacology
Glycosaminoglycans metabolism
Hydrogen-Ion Concentration
Membranes, Artificial
Microscopy, Electron, Scanning
Oxidation-Reduction drug effects
Permeability drug effects
Porosity drug effects
Solutions
Time Factors
Electrochemistry methods
Tissue Scaffolds
Subjects
Details
- Language :
- English
- ISSN :
- 1742-7061
- Volume :
- 4
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Acta biomaterialia
- Publication Type :
- Academic Journal
- Accession number :
- 18082477
- Full Text :
- https://doi.org/10.1016/j.actbio.2007.10.009