151. S100A10 silencing suppresses proliferation, migration and invasion of ovarian cancer cells and enhances sensitivity to carboplatin
- Author
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Zhumei Cui, Xukun Li, Wei Yan, Zhihua Liu, Liang Zou, Tanxiu Chen, Lingzhi Wang, and Tian Tian
- Subjects
0301 basic medicine ,endocrine system diseases ,medicine.medical_treatment ,Proliferation ,Apoptosis ,Kaplan-Meier Estimate ,Mice, SCID ,Metastasis ,Carboplatin ,chemistry.chemical_compound ,0302 clinical medicine ,Cell Movement ,Mice, Inbred NOD ,Annexin A2 ,Ovarian Neoplasms ,Predictive marker ,S100 Proteins ,Obstetrics and Gynecology ,Cell migration ,Middle Aged ,female genital diseases and pregnancy complications ,Oncology ,030220 oncology & carcinogenesis ,Female ,Antineoplastic Agents ,lcsh:Gynecology and obstetrics ,03 medical and health sciences ,Ovarian cancer ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Gene Silencing ,lcsh:RG1-991 ,Cell Proliferation ,Chemotherapy ,Wound Healing ,Oncogene ,business.industry ,Cell growth ,Research ,medicine.disease ,030104 developmental biology ,HEK293 Cells ,chemistry ,S100A10 ,Cancer research ,business - Abstract
Background Ovarian cancer is the leading cause of gynecological cancer-related mortality. The novel oncogene S100A10 has been reported to be involved in cancer cell proliferation, invasion and metastasis. The role of S100A10 in ovarian cancer has not been well studied and the effect of S100A10 on chemotherapy remains unclear. The aims of the present study were to investigate the functional role of S100A10 in the progression and carboplatin sensitivity of ovarian cancer. Methods We examined the expression levels in tissues of S100A10 in 138 cases of ovarian cancer by IHC. To determine the functional roles of downregulated S100A10 in ovarian cancer, cell proliferation, colony formation, cell migration and invasion assays were performed. Chemoresistance was analyzed by apoptosis assay. A xenograft tumor model was established to confirm the role of S100A10 in carboplatin resistance in vivo. Using Western blot assays, we also explored the possible mechanisms of S100A10 in ovarian cancer. Results The results showed that increased expression of S100A10 was positively associated with carboplatin resistance (P P = 0.048) and a poorer prognosis (P = 0.0053). Functional analyses demonstrated that S100A10 suppression significantly suppressed ovarian cancer cell proliferation, colony formation, cell migration and invasion, remarkably increased carboplatin-induced apoptosis in SKOV3 and A2780 cells and inhibited tumor growth in vivo. Downregulation of S100A10 expression could inhibit cell proliferation and enhance ovarian cancer cell sensitivity to carboplatin, possibly involving the regulation of cleaved-Caspase3 and cleaved-PARP. Conclusions Together, the results of the present study reveal that S100A10 expression can be used as a predictive marker for the prognosis of ovarian cancer and chemosensitivity to carboplatin.
- Published
- 2019