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Persulfate (PS) activation on biochar (BC) is a promising technology for degrading the aqueous organic contaminants. However, the complexity of activation mechanisms and components in biomass that used to produce BC makes it difficult to predict the performance of PS activation. In this study, we employed eight sludges as the representative biomass that contained absolutely different organic or inorganic components. Results showed that the elemental composition, surface properties, and structures of the sludge-derived BCs (SBCs) clearly depended on the inherent components in the sludges. The intensities of persistent free radicals (PFRs) in the electron paramagnetic resonance (EPR) correlated positively with N-containing content of sludges as electron shuttle, but negatively with the metal content as electron acceptor. Linking with PFRs as crucial sites of triggering a radical reaction, a poly-parameter relationship of predicting PS activation for organic degradation using the sludge components was established (kobs,PN = 0.004 × Cprotein + 0.16 × CM-0.895 -0.118). However, for the PS activation on those SBCs without PFRs, this redox process only relied on the sorption or conductivity-related characteristics, not correlating with the content of intrinsic components in biomass but with pyrolysis temperatures. This study provided insightful information of predicting the remediation efficiency of PS activation on BCs and further understanding the fate of contaminants and stoichiometric efficiency of oxidants in a field application.

Sludge-derived biochar (SDBC) has been regarded as persulfate (PS) activator during the remediation of organic contamination. However, the complexity of sludge composition makes it difficult to predict the activity of SDBC and the efficacy of PS. To improve the understanding of how the composition of sludge regulated activity of its parent SDBC towards PS activation, we used two SDBCs derived from different sludges with significantly different organic compositions and metals. Results indicated the higher content of organic and nitrogen content in sludge led to higher polymerization and condensation of carbon layer and more moieties in SDBC1, whereas more Fe species (e.g. Fe-O, Fe2+ and Fe3+) formed in SDBC2. According to the results of phenol (PN) degradation in SDBC/PS, the apparent rate constants (kobs) of SDBC2-700 (0.0037 min-1) was 2 folds higher than that of SDBC1-700 (0.0016 min-1), whereas the SDBC1-500 (6.0 ×10-4 min-1) exhibited higher kobs than that of SDBC2-500 (4.9 ×10-4 min-1). The difference of PS activation by different SDBCs mainly relied on generated reactive oxygen species (ROS). The persistent free radicals (PFRs) and Fe species acted as redox sites for generated ROS, which were depended on the organic compositions and involved metals in used sludges.

Nitrate is a crucial environmental pollutant, and its risk on ecosystem keeps increasing. Photocatalytic conversion of nitrate to ammonia can simultaneously achieve the commercialization of environmental hazards and recovery of valuable ammonia, which is green and sustainable for the planet. However, due to the thermodynamic and kinetic energy barriers, photocatalytic nitrate reduction usually involves a higher selectivity of the formation of nitrogen that largely limits the ammonia synthesis activity. In this work, we reported a green and facile synthesis of novel metallic ruthenium particle modified graphitic carbon nitride photocatalysts. Compare with bulk graphitic carbon nitride, the optimal sample had 2.93-fold photocatalytic nitrate reduction to ammonia activity (2.627 mg/h/gcat), and the NH3 selectivity increased from 50.77% to 77.9%. According to the experimental and calculated results, the enhanced photocatalytic performance is attributed to the stronger light absorption, nitrate adsorption, and lower energy barrier for the generation of ammonia. This work may provide a facile way to prepare metal modified photocatalysts to achieve highly efficient nitrate reduction to ammonia.

Urine with its abundant macronutrients (N-P-K) is an ideal resource for the production of fertiliser. However, the odor and pathogens in the raw urine must be removed to meet the public acceptance of urine collection systems and to enable its safe reuse as a fertiliser. In this work, real urine was collected and treated through a pilot-scale gravity-driven membrane bioreactor (GDMBR) to remove the malodorous organics and to nitrify almost 50% of the ammonia into nitrate. The stablised urine was subsequently distilled via low-cost heat localized solar evaporation (HLSE) to produce a non-odorous solid fertiliser. The developed HLSE with a small footprint can attract bulk solution into a vertical insulated space and quickly heat it up to 68 °C within 1 h. The HLSE process had vapour flux at 1.3 kg m-2 h-1 as well as high solar to vapour conversion efficiency at 87%. Based on the EDX mapping and XRD analysis, the generated crystals are mainly NaNO3, NH4Cl, NaCl, NH4H2PO4 and K2HPO4, which are ideal nutrients for vegetation. In this study, the produced urine-derived fertilisers have a better performance on the growth of the leafy basil than the all-purpose commercial fertilisers. Generally, the GDMBR-HLSE is a promising cost-effective and green technology for nutrients recovery from urine.

Sodium dodecylbenzene sulfonate (SDBS), a typical surfactant being widely used in various applications, was highly accumulated in waste activated sludge. To date, however, its effect on hydrogen production from dark fermentation of sludge has not been documented. The work therefore aimed to explore whether and how SDBS affects hydrogen production. Experimental results showed that with an increase of SDBS from 0 to 30 mg/g TSS, the maximal hydrogen yield increased from 2.47 to 10.73 mL/g VSS (without any treatment) and from 13.05 to 23.51 mL/g VSS (under free ammonia pretreatment). Mechanism exploration showed that SDBS lowered surface tension, facilitated organics transfer from solid to liquid. SDBS also destroyed hydrogen bonding networks of protein, promoted macromolecular organics degradation. Besides, SDBS improved the electric charge in organics, then weakened the mutual repulsion, improved adsorb, interact and promoted the availability of reaction sites between anaerobes and organic substances. Enzyme activity analysis showed that SDBS not only improved the activities of enzymes related to hydrolysis and acidification processes, but also inhibited the activities of homoacetogens and methanogens. SDBS presence lowered sludge ORP and created an environment which was helpful to the growth of butyric-type bacteria, thus enhanced butyric-type fermentation, which contributed hydrogen production largely. Microbial community analysis revealed that SDBS existence affected distributions of microbial populations, and increased the abundances of hydrogen producing microorganisms (e.g., unclassified_f_Synergistaceae). PICRUSt2 analysis showed that SDBS reduced hydrogenotrophic methanogens activity for its inhibitory effect on the biotransformation of 5,10-Methenyl-THMPT to 5-methyl-THMPT.

Clarithromycin retained in waste activated sludge (WAS) inevitably enters the anaerobic digestion system. So far, the complex impacts and fate of clarithromycin in continuous operated WAS anaerobic digestion system are still unclear. In this study, two semi-continuous long-term reactors were set up to investigate the effect of clarithromycin on biogas production and antibiotic resistance genes (ARGs) during WAS anaerobic digestion, and a batch test was carried out to explore the potential metabolic mechanism. Experimental results showed that clarithromycin at lower concentrations (i.e., 0.1 and 1.0 mg/L) did not affect biogas production, whereas the decrease in biogas production was observed when the concentration of clarithromycin was further increased to 10 mg/L. Correspondingly, the relative abundance of functional bacteria in WAS anaerobic digestion (i.e., Anaerolineaceae and Microtrichales) was reduced with long-term clarithromycin exposure. The investigation of ARGs suggested that the effect of methylation belonging to the target site modification played a critical role for the anaerobic microorganisms in the expression of antibiotic resistance, and ermF, played dominated ARGs, presented the most remarkable proliferation. In comparison, the role of efflux pump was weakened with a significant decrease of two detected efflux genes. During WAS anaerobic digestion, clarithromycin could be partially degraded into metabolites with lower antimicrobial activity including oleandomycin and 5-O-desosaminyl-6-O-methylerythronolide and other metabolites without antimicrobial activity.

Zinc oxide nanoparticles (ZnO NPs) are widespread emerging pollutants raising global concerns about their influences on biological wastewater treatment processes. However, the impacts of ZnO NPs on aerobic sludge digestion that is a major sludge treatment process remain unknown. Herein, this study comprehensively investigated the key influences of ZnO NPs on aerobic digestion of waste activated sludge (WAS) and the potential mechanisms involved. Two different entering pathways, i.e., ZnO NPs directly entered into aerobic sludge digester and ZnO NPs initially entered into wastewater bio-treatment reactor, were tested to evaluate the different impacts. Compared to the control, ZnO NPs initially entering into wastewater bioreactor inhibited WAS degradation by 18.2 ± 0.1%, whereas ZnO NPs immediately entered into digester inhibited it by 29.7 ± 0.1%. This was accompanied by a similar decrease in inorganic nitrogen production and oxygen consumption. ZnO NPs exposure in wastewater bioreactor changed WAS characteristics in favor of solubilization in aerobic digestion. Modelling analysis indicated that ZnO NPs inhibited WAS hydrolysis, especially for their direct entering into aerobic digester. Correspondingly, microbial community was shifted in the direction against aerobic digestion by the ZnO NPs. Excessive oxidative stress and Zn2+ release represented the primary toxicity factors for the inhibition.

The entering of the widespread polyethylene terephthalate (PET) microplastics into biological wastewater treatment system results in their retention in sewage sludge, which inevitably enters the sludge treatment system. However, all previous studies regarding the impact of microplastics on sludge treatment system were conducted by directly adding microplastics to system and focusing on anaerobic sludge digestion, although PET microplastics commonly enter into the biological wastewater treatment system first before sludge being subsequently treated. The potential impact of the microplastics on waste activated sludge (WAS) aerobic digestion is also completely missing. Therefore, herein the influences of PET microplastics with different entry paths on WAS aerobic digestion as well as the key mechanisms involved was firstly explored. Experimental results demonstrated that compared to the control test, the entering of PET microplastics to biological wastewater treatment system inhibited WAS aerobic digestion by 10.9 ± 0.1% through the decreased hydrolysis, although WAS solubilization during aerobic digestion was improved due to the change of generated WAS characteristics. In contrast, when PET microplastics was directly added to the sludge aerobic digester, there was little impact on solubilization, while the hydrolysis were inhibited seriously, thereby suppressing WAS aerobic digestion more severely by 28.9 ± 0.1%. Further investigation revealed that PET microplastics reduced the populations of key bacteria (e.g., Saprospiraceae, Chitinophagaceae and Xanthomonadaceae) involved in aerobic digestion via induced oxidative stress or/and releasing toxic chemical. This study provided a more accurate approach to assessing the real situation regarding the influences of PET microplastics on aerobic sludge digestion.

This study proposed a novel intermittent-aeration constructed wetland (CW) to resolve the vertical loss of oxygen in tertiary treatment. Compared to the non-aeration CW, the intermittent-aeration CW presented a better removal performance (90.8% chemical oxygen demand, 94.3% ammonia nitrogen, 91.5% total nitrogen and 94.1% total phosphorus) at a dissolved oxygen of 3 mg L-1 and hydraulic retention time of 2 days. It was mainly attributed to the higher abundance and greater diversity of bacterial community due to the oxygen supply. High-throughput sequencing indicated that high abundance of phyla Proteobacteria (35.34%) and Bacteroidetes (18.20%) in intermittent-aeration CW were responsible for simultaneous nitrogen and phosphorus removal. Besides, the dominant families Burkholderiaceae (11.16%), Microtrichales (6.88%) and Saprospiraceae (6.50%) were also detected, which was vital to hydrolyze and utilize complex organic matters. In general, oxygen supply upregulated the metabolism pathways of amino acid and carbohydrate, bringing a greater biodegradation potential for removing contaminants.

Previous chapters introduce the solar energy conversion via inorganic media, e.g. H2O2, CO2, and N2, etc. (Chapters 9–13). Biomass is a renewable resource that can be used to produce fuels and value-added chemicals. Photocatalytic and photoelectrochemical (PEC) reforming of biomass are newly emerging approaches, which can be conducted in relatively mild conditions. This chapter firstly highlights the recent works related to the photocatalytic conversion of processed and native lignin, carbohydrates, native lignocellulose, glycerides and glycerol to hydrogen, and value-added chemicals, under visible light or UVA light irradiations. For these photocatalytic processes, the strategies to treat lignin, carbohydrates, native lignocellulose, and glycerol are then concluded, including the efficient photocatalysts used and selectivity for valuable products. Afterward, the state-of-the-art accomplishments are also reviewed on the PEC reforming of biomass to electricity, hydrogen, and biomass-derived molecules such as glycerol, alcohols, as well as converting 5-hydroxymethylfurfural to corresponding valuable chemicals. For these PEC reforming processes, the recent developments on photoelectrodes and different types of PEC cells are finally highlighted.

Hydrogen production from waste activated sludge (WAS) was widely considered and intensively investigated as a promising technology to recover energy from wastewater treatment plants. To date, no efforts have been made on either systematic summarization or critical thinking of the application niche of hydrogen production from WAS treatment. It is therefore time to evaluate whether and how to recover hydrogen in a future paradigm of WAS treatment. In this critical review, the principles and potentials, microorganisms, possible technologies, and process parameters of hydrogen generation were analyzed. Microbial electrolysis cell shows high theoretical hydrogen yield and could utilize a variety of organic compounds as substrates, which is regarded as a prospective technology for hydrogen production. However, the poor organics utilization and rapid consumptions of produced hydrogen hindered hydrogen recovery from WAS. Based on the analysis of the current state of the literatures, the opportunities and challenges of hydrogen production from WAS are rethought, the detailed knowledge gaps and perspective of hydrogen production from WAS were discussed, and the probable solutions of hydrogen recovery from WAS treatment are figured out. To guide the application and development of hydrogen recovery, a more promising avenue through rational integration of the available technologies to form a hybrid process is finally proposed. The integrated operational paradigm of WWTPs could achieve substantial technical, environmental and economic benefits. In addition, how this hybrid process works is illustrated, the challenges of this hybrid process and future efforts to be made in the future are put forward.

Anaerobic co-digestion is an attractive option to treat food waste and waste activated sludge, which is increasingly applied in real-world situations. As an active component in Capsicum species being substantially present in food waste in many areas, capsaicin has been recently demonstrated to inhibit the anaerobic co-digestion. However, the interaction between capsaicin and anaerobic co-digestion are still poorly understood. This work therefore aims to deeply understand the fate and impact of capsaicin in the anaerobic co-digestion. Experiment results showed that capsaicin was completely degraded in anaerobic co-digestion by hydroxylation, O-demethylation, dehydrogenation and doubly oxidization, respectively. Although methane was proven to be produced from capsaicin degradation, the increase in capsaicin concentration resulted in decrease in methane yield from the anaerobic co-digestion. With an increase of capsaicin from 2 ± 0.7 to 68 ± 4 mg/g volatile solids (VS), the maximal methane yield decreased from 274.6 ± 9.7 to 188.9 ± 8.4 mL/g VS. The mechanic investigations demonstrated that the presence of capsaicin induced apoptosis, probably by either altering key kinases or decreasing the intracellular NAD+/NADH ratio, which led to significant inhibitions to hydrolysis, acidogenesis, and methanogenesis, especially acetotrophic methanogenesis. Illumina Miseq sequencing analysis exhibited that capsaicin promoted the populations of complex organic degradation microbes such as Escherichia-Shigella and Fonticella but decreased the numbers of anaerobes relevant to hydrolysis, acidogenesis, and methanogenesis such as Bacteroide and Methanobacterium.

The susceptibility of the completely autotrophic nitrogen removal over nitrite (CANON) process to high salinity limits its widespread application. The addition of glycine betaine (GB), a type of compatible solutes that could resist osmotic stress, could be an effective strategy to enhance the salt tolerance ability of aerobic and anaerobic ammonium oxidizing bacteria (AOB and anammox bacteria) involved in the CANON process. This study aims to make use of mathematical modeling to systematically investigate the effects of salt and GB addition on the activities of AOB and anammox bacteria and the treatment performance of the CANON process. To this end, a series of dedicated batch tests and long-term experiments for the CANON process with salt and GB additions were conducted and the data was used to calibrate and validate the model established to consider the relationships between salt and GB concentrations and bacterial growth in the CANON process. The calibrated/validated CANON process model was then applied to simulate the long-term impacts of GB addition concentration and sludge retention time (SRT) on the CANON process. The results showed that 1 mM GB addition and a SRT of 50 days would be sufficient to protect AOB and anammox bacteria under the high salinity (30 g/L NaCl) conditions studied and therefore reduce the time needed to recover the treatment performance of the CANON process from exposure to salt inhibition by 35%-40%.

Anaerobic ammonium oxidation (anammox) represents a promising technology for wastewater nitrogen removal. Organics management is critical to achieving efficient and stable performance of anammox or integrated processes, e.g., denitratation-anammox. The aim of this systematic review is to synthesize the state-of-the-art knowledge on the multifaceted impacts of organics on wastewater anammox community structure and function. Both exogenous and endogenous organics are discussed with respect to their effects on the biofilm/granule structure and function, as well as the interactions between anammox bacteria (AnAOB) and a broad range of coexisting functional groups. A global core community consisting of 19 taxa is identified and a co-occurrence network is constructed by meta-analysis on the 16S rDNA sequences of 149 wastewater anammox samples. Correlations between core taxa, keystone taxa, and environmental factors, including COD, nitrogen loading rate (NLR) and C/N ratio are obtained. This review provides a holistic understanding of the microbial responses to different origins and types of organics in wastewater anammox reactors, which will facilitate the design and operation of more efficient anammox-based wastewater nitrogen removal process.

Due to the environmental risks caused by microplastics, understanding the sources and characteristics of microplastics and cutting off their routes into the environment are crucial. However, so far, studies on microplastics in the landfill leachate system (a major pathway of microplastics into the environment) are still limited, especially for tiny particles <50 µm that might have higher risks to the environment. This study investigated the microplastics in landfill leachate and in leachate treatment works, with a size detection limit down to 10 µm. The results showed that the microplastics particle and mass concentrations in the untreated leachate were 235.4 ± 17.1 item/L and 11.4 ± 0.8 µg/L, respectively, with tiny particles (<50 µm) accounting for over 50%. Overall, 27 polymeric materials were detected in leachate samples, with polyethylene and polypropylene being the most abundant in the untreated leachate. The neutral buoyancy of microplastics (average density: 0.94 g/cm3), together with irregular shapes, suggested they may be difficult to be removed by sedimentation. Further exploring the fate of microplastics in leachate treatment works showed that the membrane treatment effectively reduced microplastics loading to 0.14% for particle and 0.01% for mass, but the average particle density rose. The differences in polymeric materials distribution at different sampling locations and the presence of membrane-related polymer in membrane treatment effluent suggested tiny microplastics could be generated and released from membrane systems. Moreover, this study discovered that the sludge dewatering liquor could contain a high amount of microplastics, and the estimated particle loading was about 3.6 times higher than that in dewatered sludge. This suggested a new approach to microplastics mitigation through separating microplastics from the sludge dewatering liquor before its recirculation.

Chemical absorption-biological reduction based on Fe(II)EDTA is a promising technology to remove nitric oxide (NO) from flue gases. However, limited effort has been made to enable direct energy recovery from NO through production of nitrous oxide (N2O) as a potential renewable energy rather than greenhouse gas. In this work, the enhanced energy recovery in the form of N2O via biological NO reduction was investigated by conducting short-term and long-term experiments at different Fe(II)EDTA-NO and organic carbon levels. The results showed both NO reductase and N2O reductase were inhibited at Fe(II)EDTA-NO concentration up to 20 mM, with the latter being inhibited more significantly, thus facilitating N2O accumulation. Furthermore, N2O accumulation was enhanced under carbon-limiting conditions because of electron competition during short-term experiments. Up to 47.5% of NO-N could be converted to gaseous N2O-N, representing efficient N2O recovery. Fe(II)EDTA-NO reduced microbial diversity and altered the community structure toward Fe(II)EDTA-NO-reducing bacteria-dominated culture during long-term experiments. The most abundant bacterial genus Pseudomonas, which was able to resist the toxicity of Fe(II)EDTA-NO, was significantly enriched, with its relative abundance increased from 1.0 to 70.3%, suggesting Pseudomonas could be the typical microbe for the energy recovery technology in NO-based denitrification.

Microplastics in wastewater inevitably accumulate in waste activated sludge (WAS) via wastewater biological treatment, potentially affecting the subsequent sludge treatment unit. Nevertheless, all previous research studies focused on the impacts of the direct addition of one type of model microplastics on the sludge anaerobic treatment process. This approach actually cannot reflect the real situation where multiple different microplastics simultaneously get into the wastewater treatment unit prior to the sludge treatment unit. Herein, this work innovatively proposed a more realistic method to assess the real toxic influences of microplastics on anaerobic WAS fermentation for short-chain fatty acid (SCFA) production by initially adding four typical microplastics (i.e., polyethylene terephthalate, polystyrene, and polypropylene) to the biological wastewater treatment system. Results showed that four microplastics initially entering the biological wastewater treatment reactor had little influence on the subsequent anaerobic SCFA production since WAS solubilization increased but hydrolysis and acidification decreased. In contrast, when the four microplastics were directly dosed in a WAS anaerobic fermenter, although there was no effect on WAS solubilization, the bioprocess of hydrolysis-acidification was clearly suppressed, ultimately significantly (P = 1.86 × 10-7) inhibiting the maximal SCFA production from WAS by 21.5 ± 0.1% compared to the control without microplastic addition. The excessive oxidative stress and toxic leachates from these typical microplastics reduced the relative abundances of key anaerobes (e.g., Longilinea sp.) involved in the anaerobic fermentation. This work revealed that the different pathways of microplastics entering the sludge treatment system had different impacts on anaerobic sludge fermentation processes and selecting a more realistic and accurate approach was important to evaluate the true toxicity of microplastics on the sludge anaerob

Hydrothermal carbonization (HTC) is an eco-friendly, flexible and efficient way to valorise wet solid wastes, producing a carbon-rich material named as hydrochar. Considerable efforts have been devoted to studying the feasibility of using hydrochar in waste management to achieve the goal of circular economy. However, a comprehensive evaluation of the impacts of hydrochar on energy recovery from anaerobic digestion (AD), nutrient reclamation, and wastewater treatment is currently lacking. To understand the influence of hydrochar type on its application, this review will firstly introduce the mechanisms and biomass treatment for hydrochar preparation. Most recent studies regarding the improvement of methane (CH4) and volatile fatty acids (VFAs) production after dosing hydrochar in anaerobic digesters are quantitatively summarized and deeply discussed. The potential of using various hydrochar as slow-fertilizer to support the growth of plants are analysed by providing quantitative data. The usage of hydrochar in remediating pollutants from wastewater as effective adsorbent is also evaluated. Based on the review, we also address the challenges and demonstrate the opportunities for the future application of hydrochar in waste management. Conclusively, this review will not only provide a systematic understanding of the up-to-date developments of improving the nutrients removal and energy recovery from wastes by using hydrochar but also several new directions for the application of hydrochar in the future.

Hydrogen, with zero-carbon footprint, high energy density, and earth abundance, is proved as a great energy carrier for a sustainable energy scheme, which is recognized as one key solution to mitigate climate change and reduce air pollution. To achieve this goal, reducing the cost of renewable hydrogen production via electrochemical water splitting is a requisite for supporting a reliable and affordable hydrogen economy. Thus, the development of cost-effective catalysts for water electrolysis is of great significance. In this review, the recent advances in low-cost electrocatalysts for water splitting are summarized, including transition metal–based catalysts and metal-free catalysts. The emphasis is put on the catalyst design strategies and the underlying structure–performance mechanisms. The challenges and perspectives in this booming field are also presented.

This study proposed a novel method to enhance short-chain fatty acids (SCFAs) production from anaerobic algae fermentation by using coconut shell ash. The maximum SCFAs production was 683.0 mg COD/g VS at the ash dosage of 1.2 g/g TS, which was about 1.4-folds that of the control, and the enhancement of acetate production was the main path for the promotion of SCFAs. Coconut shell ash increased the pH and alkalinity of digestate, thereby reducing the use of alkaline reagents and being more resistant to acidic environments. Coconut shell ash promoted the processes of solubilization, hydrolysis and acetogenesis, and enriched hydrolytic microorganisms (e.g., Candidatus Microthrix) and acidifying microorganisms with acetate as substrate (e.g., Caldilinea and Proteiniphilum). Anaerobic fermentation residue with ash containing inorganic elements has the potential to be used as fertilizer, making this waste-control-waste strategy with more economic and environmental benefits for potential practical applications.

Poly ferric sulfate (PFS), one of the typical inorganic flocculants widely used in wastewater management and waste activated sludge (WAS) dewatering, could be accumulated in WAS and inevitably entered in anaerobic digestion system at high levels. However, knowledge about its impact on methane production is virtually absent. This study therefore aims to fill this gap and provide insights into the mechanisms involved through both batch and long-term tests using either real WAS or synthetic wastewaters as the digestion substrates. Experimental results showed that the maximum methane potential and production rate of WAS was respectively retarded by 39.0% and 66.4%, whereas the lag phase was extended by 237.0% at PFS of 40 g per kg of total solids. Mechanism explorations exhibited that PFS induced the physical enmeshment and disrupted the enzyme activity involved in anaerobic digestion, resulting in an inhibitory state of the bioprocess of hydrolysis, acidogenesis, and methanogenesis. Furthermore, PFS's inhibition to hydrogenotrophic methanogenesis was much severer than that to acetotrophic methanogenesis, which could be supported by the elevated abundances of Methanosaeta sp and the dropped abundances of Methanobacterium sp in PFS-present digester, and probably due to the severe mass transfer resistance of hydrogen between the syntrophic bacteria and methanogens, as well as the higher hydrogen appetency of PFS-induced sulfate reducing bacteria. Among the derivatives of PFS, "multinucleate and multichain-hydroxyl polymers" and sulfate were unveiled to be the major contributors to the decreased methane potential, while the "multinucleate and multichain-hydroxyl polymers" were identified to be the chief buster to the slowed methane-producing rate and the extended lag time.

Integrated Membrane Bioreactor (MBR) models, combination of biological and physical models, have been representing powerful tools for the accomplishment of high environmental sustainability. This paper, produced by the International Water Association (IWA) Task Group on Membrane Modelling and Control, reviews the state-of-the-art, identifying gaps for future researches, and proposes a new integrated MBR modelling framework. In particular, the framework aims to guide researchers and managers in pursuing good performances of MBRs in terms of effluent quality, operating costs (such as membrane fouling, energy consumption due to aeration) and mitigation of greenhouse gas emissions.