151. Seroprevalence and Molecular Detection of Bovine Anaplasmosis in Egypt
- Author
-
Heinrich Neubauer, Katja Mertens-Scholz, Falk Melzer, Uwe Roesler, Omid Parvizi, and Hosny El-Adawy
- Subjects
Microbiology (medical) ,Veterinary medicine ,medicine.medical_specialty ,040301 veterinary sciences ,animal diseases ,030231 tropical medicine ,prevalence ,lcsh:Medicine ,600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::616 Krankheiten ,coxiella burnetii ,Article ,0403 veterinary science ,03 medical and health sciences ,0302 clinical medicine ,Epidemiology ,parasitic diseases ,medicine ,TaqMan ,Immunology and Allergy ,Seroprevalence ,Anaplasma ,anaplasma marginale ,Molecular Biology ,General Immunology and Microbiology ,biology ,Ehrlichia ,Bovine anaplasmosis ,lcsh:R ,04 agricultural and veterinary sciences ,biology.organism_classification ,Coxiella burnetii ,medicine.disease ,bacterial infections and mycoses ,Anaplasma marginale ,real time PCR ,Infectious Diseases ,biology.protein ,bacteria ,Egypt ,ELISA ,Antibody ,Anaplasmosis ,600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::636 Viehwirtschaft - Abstract
Bovine anaplasmosis is a tick-borne disease with zoonotic potential, caused by the obligate intracellular bacterium Anaplasma marginale. The disease is distributed worldwide in tropical and subtropical regions. The economic losses from anaplasmosis in animals is of significant importance because it causes severe morbidity and mortality in cattle. Recovered animals may become persistent carriers. Epidemiological information on the actual status of bovine anaplasmosis in Egypt is scarce. Thus, this study aimed to determine anti-Anaplasma antibody and DNA in serum samples using ELISA and PCR, respectively. In total, 758 bovine sera were collected from cattle farms located in 24 Egyptian governorates in 2015 to 2016. Sera were analyzed with the commercially available &lsquo, Anaplasma antibody competitive ELISA v2&rsquo, kit and &lsquo, AmpliTest Anaplasma/Ehrlichia spp. real time TaqMan TM PCR. Anaplasma spp. antibodies were detected in 140 (18.5%) (CI: 15.8&ndash, 21.4%) of the investigated sera by ELISA, and Anaplasma/Ehrlichia-DNA was detected in 40 (5.3%) (CI: 3.8&ndash, 7.1%) of the positive sera by real time PCR. Co-detection of both Anaplasma spp. and Coxiella burnetii-specific antibodies was proven in 30 (4%) of the investigated sera. The results of this work confirm the significant prevalence of bovine anaplasmosis in Egypt. Raising awareness in decision makers of the public health, veterinarians and animal owners is required to reduce the spread of infection.
- Published
- 2019