Back to Search
Start Over
Evolution of Antibiotic Resistance of Coagulase-Negative Staphylococci Isolated from Healthy Turkeys in Egypt: First Report of Linezolid Resistance
- Source :
- Microorganisms, Vol 7, Iss 10, p 476 (2019), Microorganisms, Volume 7, Issue 10
- Publication Year :
- 2019
- Publisher :
- MDPI AG, 2019.
-
Abstract
- Coagulase-negative staphylococci (CoNS) are gaining much attention as causative agents of serious nosocomial infections in humans. This study aimed to determine the prevalence and phenotypic antimicrobial resistance of CoNS as well as the presence of resistance-associated genes in CoNS isolated from turkey farms in Egypt. Two hundred and fifty cloacal swabs were collected from apparently healthy turkeys in Egypt. Suspected isolates were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The susceptibility testing of CoNS isolates against 20 antimicrobial agents was performed using the broth microdilution test. The presence of resistance-associated genes like mecA, vanA, blaZ, erm(A), erm(B), erm(C), aac-aphD, optrA, valS, and cfr was determined. Thirty-nine CoNS were identified. All isolates were phenotypically resistant to trimethoprim/sulfamethoxazole, penicillin, ampicillin, and tetracycline. The resistance rates to erythromycin, chloramphenicol, oxacillin, daptomycin, and tigecycline were 97.4%, 94.9%, 92.3%, 89.7%, and 87.2%, respectively. Thirty-one isolates were resistant to linezolid (79.5%). Low resistance rate was detected for both imipenem and vancomycin (12.8%). The erm(C) gene was identified in all erythromycin phenotypically resistant isolates, whereas two resistant isolates possessed three resistance-conferring genes erm(A), erm(B), and erm(C). The cfr and optrA genes were detected in 11 (35.5%) and 12 (38.7%) of the 31 linezolid-resistant isolates. The mecA, aac-aphD, and blaZ genes were identified in 22.2%, 41.9%, and 2.6% of phenotypically resistant isolates to oxacillin, gentamicin, and penicillin, respectively. This is the first study revealing the correlation between linezolid resistance and presence of cfr and optrA genes in CoNS isolates from Egypt, and it can help to improve knowledge about the linezolid resistance mechanism.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Imipenem
030106 microbiology
Erythromycin
Tigecycline
linezolid
Biology
Microbiology
Article
03 medical and health sciences
chemistry.chemical_compound
Antibiotic resistance
Virology
Ampicillin
medicine
turkey
egypt
lcsh:QH301-705.5
staphylococci
biochemical phenomena, metabolism, and nutrition
bacterial infections and mycoses
Penicillin
030104 developmental biology
chemistry
lcsh:Biology (General)
Linezolid
cons
Coagulase
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 20762607
- Volume :
- 7
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Microorganisms
- Accession number :
- edsair.doi.dedup.....4eafc3b38a50bce4ee7c390c138f10ec