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Due to the errors of the servo system and the errors of the ball screw drive system, the positioning errors inevitably occur in the process of CNC machine tools. The measurement of traditional equipment is limited by a fixed measurement radius and a single degree of freedom, which can only be measured within a fixed plane. In this paper, four different positioning errors of CNC machine tools are first measured at full scale by using J-DBB (a modified double ball bar with one spherical joint connecting two bars) method. The J-DBB device uses a three-degree-of-freedom spherical joint as a connecting part, which realizes that the measurement radius can be continuously changed, and the measurement space is a spatial sphere. First, the principle of the J-DBB method is briefly introduced. Next, four typical positioning errors of CNC machine tools are analyzed and examined, which contain the uniform contraction error of ball screw and linear grating, periodic error of the ball screw and linear grating, interference of measurement devices error, and opposite clearance error. In the end, the trajectories of the CNC machine tool spindle with a single positioning error are simulated by using the J-DBB method. The results reveal that this method can be used for the positioning error of machine tools, which helps to better understand the spatial distribution of CNC machine tool errors and provides guidance for the reasonable selection of working areas to improve the machining accuracy of parts.

Cylindrical shells are principal structural elements that are used for many purposes, such as offshore, sub-marine, and airborne structures. The nonlinear mechanics model of internal blast loading was established to predict the dynamic blast pressure of cylindrical shells. However, due to the complexity of the nonlinear mechanical model, the solution process is time-consuming. In this study, the nonlinear mechanics model of internal blast loading is linearized, and the dynamic blast pressure of cylindrical shells is solved. First, a mechanical model of cylindrical shells subjected to internal blast loading is proposed. To simplify the calculation, the internal blast loading is reduced to linearly uniform variations. Second, according to the stress function method, the dynamic blast pressure equation of cylindrical shells subjected to blast loading is derived. Third, the calculated results are compared with those of the finite element method (FEM) under different durations of dynamic pressure pulse. Finally, to reduce the errors, the dynamic blast pressure equation is further optimized. The results demonstrate that the optimized equation is in good agreement with the FEM, and is feasible to linearize the internal blast loading of cylindrical shells.

Background/Aims: Recent studies provided compelling evidence that stimulation of the calcium sensing receptor (CaSR) exerts direct renoprotective action at the glomerular podocyte level. This protective action may be attributed to the RhoA-dependent stabilization of the actin cytoskeleton. However, the underlying mechanisms remain unclear. Methods: In the present study, an immortalized human podocyte cell line was used. Fluo-3 fluorescence was utilized to determine intracellular Ca2+ concentration ([Ca2+]i), and western blotting was used to measure canonical transient receptor potential 6 (TRPC6) protein expression and RhoA activity. Stress fibers were detected by FITC-phalloidin. Results: Activating CaSR with a high extracellular Ca2+ concentration ([Ca2+]o) or R-568 (a type II CaSR agonist) induces an increase in the [Ca2+]i in a dose-dependent manner. This increase in [Ca2+]i is phospholipase C (PLC)-dependent and is smaller in the absence of extracellular Ca2+ than in the presence of 0.5 mM [Ca2+]o. The CaSR activation-induced [Ca2+]i increase is attenuated by the pharmacological blockage of TRPC6 channels or siRNA targeting TRPC6. These data suggest that TRPC6 is involved in CaSR activation-induced Ca2+ influx. Consistent with a previous study, CaSR stimulation results in an increase in RhoA activity. However, the knockdown of TRPC6 significantly abolished the RhoA activity increase induced by CaSR stimulation, suggesting that TRPC6-dependent Ca2+ entry is required for RhoA activation. The activated RhoA is involved in the formation of stress fibers and focal adhesions in response to CaSR stimulation because siRNA targeting RhoA attenuated the increase in the stress fiber mediated by CaSR stimulation. Moreover, this effect of CaSR activation on the formation of stress fibers is also abolished by the knockdown of TRPC6. Conclusion: TRPC6 is involved in the regulation of stress fiber formation and focal adhesions via the RhoA pathway in response to CaSR activation. This may explain the direct protective action of CaSR agonists.

Abstract This paper deals with the existence and approximate controllability for a class of fractional nonlocal control systems governed by abstract fractional evolution equations with multiple delays. Under some weaker assumptions, the existence as well as the approximate controllability is established by using fixed point theory. An example is given to illustrate the applicability of the abstract results.

Abstract In this paper, we first prove some coupled fixed point theorems in partially ordered Φ-orbitally complete normed linear spaces. And then apply the obtained fixed point theorems to a class of semilinear evolution systems of fractional order for proving the existence of coupled mild solutions under some weaker monotone conditions. An example is given to illustrate the application of the abstract results.

In order to enhance the capability of resistant to protein adsorption of the copolymer membrane of polyacryloylmorpholine-graft-acryloylmorpholine(PVDF-g-PACMO), a crosslinking agent of ethylene glycoldimethacrylate(EGDMA) was added to prepare the copolymer.The copolymers with different proportions of the crosslinking agent were cast into flat membranes via a liquid-liquid phase separation technique, and then the influence of EGDMA contained in the membranes on its capability of resistant to protein adsorption was investigated. FT-IR results indicate the PVDF-g-PACMO copolymers with EGDMA are synthesized successfully. A series of tests, including XPS, SEM, the contact angle, the static protein adsorption and permeation experiments, show that PACMO is much easier to segregate to the surface with the increase of the crosslinking agent content in the system, the number of membrane pores increases, meantime, the hydrophilicity and water flux of the copolymer membranes are improved. Moreover, the adsorption amount of protein, total pollution index and irreversible pollution index decrease, and reversible pollution index increases. All results indicate the crosslinking agent of EGDMA can enhance the capability of resistant to protein adsorption of PVDF-g-PACMO membranes.

The antidepressant-like effect of the sesquiterpenoids in Ginseng root was evaluated by using animal models of depression, forced swim test in mice. Our results showed that treatment with the sesquiterpenoids at dose of 1.0 mg/kg significantly decreased immobility time in the forced swimming test. Pretreatment with haloperidol (a non-selective D2 receptor antagonist), bicuculline (a competitive GABA antagonist) and N-methyl-D-aspartic acid (an agonist at the glutamate site) effectively reversed the antidepressant-like effect of the sesquiterpenoids (1.0 mg/kg). Moreover, the sesquiterpenoids treatment did not change the locomotor activity. And the sesquiterpenoids (1.0 mg/kg) also effectively increased the DA and GABA levels in mice brains exposed to the FST on the co-treatment groups. In conclusion, the sesquiterpenoids in Ginseng root (1.0 mg/kg) produced significant antidepressant-like effect, which was probably related to the dopaminergic, GABAergic and glutamatergic systems.

The phase transformations and precipitation behavior were investigated by using Thermo-Calc software in the Fe-(18-21)Cr-(3-5)Al-(0-0.03)C-(0-0.2)Si-(0-0.2)Mn multicomponent system relevant to FeCrAl stainless steel during solidification. The vertical sections of this system were calculated by using the TCFE7 database. Based on these vertical sections, the influence of different elements was analyzed in the phase transformations during solidification and a diagram of the phase-transformation path of FeCrAl stainless steel was obtained during equilibrium solidification. The results indicate that the full-phase transformation path of FeCrAl stainless steel during the cooling process from 1600℃ to 300℃ is as follows:L→AlN+αδFe→AlN+αδFe+Cr7C3→AlN+αδFe+Cr7C3+Cr23C6→AlN+αδFe+Cr23C6→AlN+αδFe+Cr23C6+σ→AlN+αδFe+Cr23C6+σ+α'→AlN+αδFe+Cr23C6+α'. The precipitation of Cr7C3 and σ, during the solidification process mainly depends on the carbon and silicon contents in the system, respectively. Increasing the aluminum content can enlarge the stable region of αδFe+Cr7C3, lower the precipitation temperature of α', and restrain σ precipitation. Increasing the chromium content can reduce the stable region of αδFe+Cr7C3 and enlarge the stable region of σ and α'.

In this study, the hydromechanical behavior of a concrete fracture under coupled compressive and shear stresses was investigated. A special experimental device was designed to create a planar fracture in a cylindrical sample and to carry out different kinds of hydromechanical tests on the fracture. Four series of laboratory tests were performed on an ordinary concrete sample. Hydrostatic compression tests were first conducted to characterize the normal compressibility of the fracture. In the second series, direct shear tests were conducted on the fracture under different normal stresses. The maximal shear stress of the fracture was determined as a function of the normal stress. In the third series, fluid flow tests were carried out in view of characterizing the overall hydraulic conductivity of the fracture as a function of its opening and closure. Shear tests with a constant fluid pressure were finally performed to investigate the influence of fluid pressure on the deformation behavior of concrete fractures. Based on the experimental investigation, an elastoplastic model is proposed. This model takes into account the nonlinear elastic behavior of a fracture under normal compression and the plastic deformation and failure due to shear stress. The model was coupled with the classical Darcy's law to describe the fluid flow along the fracture by considering the variation of permeability with fracture aperture. Numerical results agree with experimental data from various laboratory tests.

HIV-1 inserts its proviral DNA into the infected host cells, by which HIV proviral DNA can then be duplicated along with each cell division. Thus, provirus cannot be eradicated completely by current antiretroviral therapy. We have developed an innovative strategy to silence the HIV provirus by targeted DNA methylation on the HIV promoter region. We genetically engineered a chimeric DNA methyltransferase 1 composed of designed zinc-finger proteins to become ZF2 DNMT1. After transient transfection of the molecular clone encoding this chimeric protein into HIV-1 infected or latently infected cells, efficient suppression of HIV-1 expression by the methylation of CpG islands in 5′-LTR was observed and quantified. The effective suppression of HIV in latently infected cells by ZF2-DNMT1 is stable and can last through about 40 cell passages. Cytotoxic caused by ZF2-DNMT1 was only observed during cellular proliferation. Taken together, our results demonstrate the potential of this novel approach for anti-HIV-1 therapy. Keywords: HIV-1, methylation, zinc finger, chimeric protein, DNMT1, gene therapy

A truly random number generator based on thermal noise was proposed, and the resistance thermal noise was used as the noise source of the low frequency clock. It uses two independent working relative relationship between high and low frequency oscillator to sample noise source, and uses low frequency oscillator with jitter to sample the high frequency osciuator which has a fixed cycle oscillator by D flip-flop, to generate a random number sequence. The circuit was based on SMIC 28 nm CMOS process. The simulation results show that based on the thermal noise of truly random number generator the output throughput rate range is 1.8~12 Mb/s, random number output satisfies the requirement of entropy source AIS31 truly random number test, and the overall power consumption is 0.388 mW.

Electro-hydraulic servo-valves play key roles in modern electro-hydraulic power systems in many industrial applications. As one of the widely used hydraulic servo-valves, cavitation in the flapper-nozzle servo-valve could produce deleterious effects of noise, flapper vibration and cavitation erosion. In this work, a numerical investigation has been conducted on the manipulation of the flapper-nozzle pilot valve using continuous minijets. Two minijets are symmetrically deployed around the main jet of each nozzle. Vapor fraction and flow forces under the effect of the continuous minijets are compared in detail between the traditional nozzle and the nozzle with the minijets. It is found that cavitation in the flapper-nozzle stage is greatly weakened under the effect of the continuous minijets. The remarkable drop in the radial jet velocity results in the suppression of the swirling structures in the annulus, which may be responsible for the cavitation reduction. The variation trend of the flow forces with the inlet pressure remains unchanged while the magnitude of the flow forces is varied slightly, depending on the various surfaces of the flapper.

Nonlinear factors such as the contact stiffness and friction damping at the threaded interface of a projectile–fuse system significantly affect the dynamic response characteristics. To obtain the dynamic response of the fuse body accurately during penetration, it is necessary to characterize these nonlinear factors reasonably. Because the existing structural dynamics software cannot effectively deal with nonlinear factors, the thin-layer element method was used to represent the nonlinear factors in this study. By combining the thread elastic model with thin-layer element principles, an effective method for determining the material parameters of the thin-layer element was established theoretically, which provided a different method of determining material parameters, not just relying on experiments. The accuracy of the material parameters was verified based on modal experiments with threaded tubes having different specifications. The errors were within 5%, indicating the reliability of the theoretical determination method for the material parameters. In addition, projectile penetration into a semi-infinite concrete target was tested to verify the accuracy of the thin-layer element modeling. Compared with the ‘TIED’ constraint method, the resonant frequency obtained with the thin-layer element method was in better agreement with that of the experimental data. The maximum error decreased from 15.7 to 7.8%, indicating that the thin-layer element method could accurately represent the nonlinear factors. Thus, this study serves as a reference for accurately evaluating the dynamic response of the fuse body of a penetrator.

Due to the advantage in deactivating fluorine and enhancing the decomposition of rare earth (RE) minerals, MgO was applied to the magnetizing roasting of Bayan Obo tailings in this work. The effects of MgO dosages, roasting temperature, and holding time on the decomposition rate of RE minerals were experimentally studied. With a MgO dosage of 10 wt.%, the decomposition rate of RE minerals reached 98.09% at 750 °C. The phase composition of roasted samples was characterized by XRD and SEM-EDS. The incomplete decomposition rate was investigated with the observation of leaching residual by SEM-EDS. The decomposition kinetics of the RE minerals with the addition of MgO was analyzed with the Ginstling-Brundshtein model, where the reaction rate was controlled by chemical reaction.

This paper deals with the existence of ω-periodic solutions for nth-order ordinary differential equation involving fixed delay in Banach space E. Lnu(t)=f(t,u(t),u(t−τ)),t∈R, where Lnu(t):=u(n)(t)+∑i=0n−1aiu(i)(t), ai∈R, i=0,1,⋯,n−1, are constants, f(t,x,y):R×E×E⟶E is continuous and ω-periodic with respect to t, τ>0. By applying the approach of upper and lower solutions and the monotone iterative technique, some existence and uniqueness theorems are proved under essential conditions.

In the iron and steel industry, improving the usage amount of New Zealand sea sand ore as a raw material for ironmaking can reduce the production costs of iron and steel enterprises to a certain extent. In this paper, New Zealand sea sand ore without any grinding pretreatment was used as a raw material, oxidized pellets were prepared by using a disc pelletizer, and the effect of sea sand ore on the performance of green pellets and the metallurgical properties of oxidized pellets was investigated. The effects of sea sand ore on the compressive strength, falling strength, compressive strength of oxidized pellets, and reduction performance were mainly investigated. X-Ray Diffraction (XRD) patterns and Scanning Electron Microscope (SEM) analysis methods were used to discuss the influence of sea sand ore on the microstructure of the pellets’ oxidation and reduction process. As the amount of sea sand ore used increased, the compressive strength of green pellets was gradually decreased, and the falling strength of green pellets and the compressive strength of oxidized pellets were gradually increased. When the amount of sea sand ore used was 40%, the reduction swelling index of pellets was 16.31%. The increase of sea sand ore used made the reduction of pellets suppressed and the reduction rate decreased. When the amount of sea sand ore used increased to 40%, the reduction degree of sea sand ore pellets was only 60.06%. The experimental results in this paper provide specific experimental data for the large-scale application of New Zealand sea sand ore in the blast furnace ironmaking process.